Heterologous Expression of CFL1 Confers Flocculating Ability to Cutaneotrichosporon oleaginosus Lipid-Rich Cells DOI Creative Commons
Silvia Donzella, Concetta Compagno

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(12), P. 1293 - 1293

Published: Dec. 11, 2022

Lipid extraction from microbial and microalgae biomass requires the separation of oil-rich cells production media. This downstream procedure represents a major bottleneck in biodiesel production, increasing cost final product. Flocculation is rapid cheap system for removing solid particles suspension. natural characteristic displayed by some microorganisms due to presence lectin-like proteins (called flocculins/adhesins) cell wall. In this work, we showed, first time, that heterologous expression adhesin Cfl1p endows oleaginous species Cutaneotrichosporon oleaginosus with capacity flocculation. We used Helm’s test demonstrate acquisition trait allows reducing time required lipid-rich liquid culture centrifugation without altering productivity. improves lipid process remarkably providing more efficient downstream.

Language: Английский

Oleaginous microorganisms as a sustainable oil source with a focus on downstream processing and cost-lowering production strategies: A review DOI Creative Commons
Denner Nunes, Venitha Pillay, Eugéne van Rensburg

et al.

Bioresource Technology Reports, Journal Year: 2024, Volume and Issue: 26, P. 101871 - 101871

Published: May 31, 2024

The increased demand for specialty oils, driven by a growing population and thus consumption as well seasonal fluctuations, poses challenges the environment edible oil industries. Oleaginous microorganisms offer promising solution they are capable of yielding oils with high concentrations polyunsaturated fatty acids or profiles similar to that traditional fats. These have applications in diverse industries, including food, biofuels, pharmaceuticals, healthcare. While multiple studies explore strains suitable industrial-scale production, data comparison remains limited. versatility utilising various carbon sources, independence, minimal ecological footprint. However, downstream processing steps still hinder overall profitability commercialisation. This review takes bioprocess engineering approach, focusing on upstream processing, up-scale, practical economic considerations, innovative strategies developed lower production costs.

Language: Английский

Citations

9
CFSA: Comparative flux sampling analysis as a guide for strain design DOI Creative Commons
Rik P. van Rosmalen, Sara Moreno‐Paz, Zeynep Efsun Duman‐Özdamar

et al.

Metabolic Engineering Communications, Journal Year: 2024, Volume and Issue: 19, P. e00244 - e00244

Published: June 25, 2024

Genome-scale metabolic models of microbial metabolism have extensively been used to guide the design cell factories, still, many available strain algorithms often fail produce a reduced list targets for improved performance that can be implemented and validated in step-wise manner. We present Comparative Flux Sampling Analysis (CFSA), method based on extensive comparison complete spaces corresponding maximal or near-maximal growth production phenotypes. The is complemented by statistical analysis identify reactions with altered flux are suggested as genetic interventions including up-regulations, down-regulations gene deletions. applied CFSA lipids

Language: Английский

Citations

6
Recycling Food Waste and Saving Water: Optimization of the Fermentation Processes from Cheese Whey Permeate to Yeast Oil DOI Creative Commons
Silvia Donzella, Andrea Fumagalli, Stefania Arioli

et al.

Fermentation, Journal Year: 2022, Volume and Issue: 8(7), P. 341 - 341

Published: July 19, 2022

With the aim of developing bioprocesses for waste valorization and a reduced water footprint, we optimized two-step fermentation process that employs oleaginous yeast Cutaneotrichosporon oleaginosus production oil from liquid cheese whey permeate. For first step, addition urea as cost-effective nitrogen source allowed an increase in biomass production. In second syrup candied fruit processing, another food supplied carbon feeding, triggered lipid accumulation. Consequently, lipids were produced at final concentration productivity 38 g/L 0.57 g/L/h respectively, which are among highest reported values. Through this strategy, based on wastes (WP mango syrup) by recovering not only nutritional compounds but also necessary growth production, addressed one main goals circular economy. addition, set up accurate fast-flow cytometer method to quantify content, avoiding extraction step use solvents. This can represent analytical improvement screening different strains monitoring single-cell level.

Language: Английский

Citations

22
Screening of Non-Conventional Yeasts on Low-Cost Carbon Sources and Valorization of Mizithra Secondary Cheese Whey for Metabolite Production DOI Creative Commons
Gabriel Vasilakis,

Rezart Tefa,

Antonios Georgoulakis

et al.

BioTech, Journal Year: 2025, Volume and Issue: 14(2), P. 24 - 24

Published: April 1, 2025

The production of microbial metabolites such as (exo)polysaccharides, lipids, or mannitol through the cultivation microorganisms on sustainable, low-cost carbon sources is high interest within framework a circular economy. In current study, two non-extensively studied, non-conventional yeast strains, namely, Cutaneotrichosporon curvatus NRRL YB-775 and Papiliotrema laurentii Y-3594, were evaluated for their capability to grow semi-defined lactose-, glycerol-, glucose-based substrates produce value-added metabolites. Three different nitrogen-to-carbon ratios (i.e., 20, 80, 160 mol/mol) tested in shake-flask batch experiments. Pretreated secondary cheese whey (SCW) was used fed-batch bioreactor P. under nitrogen limitation. Based screening results, both strains can substrates, yielding concentrations biomass (>20 g/L) nitrogen-excess conditions, with polysaccharides comprising predominant component (>40%, w/w, dry biomass). Glucose- glycerol-based cultures C. promote secretion (13.0 g/L case glucose, nitrogen-limited conditions). lipids (maximum 2.2 produced by rich oleic acid (≥40%, w/w) could potentially be utilized second-generation biodiesel. SCW nutritionally sufficient strain, resulting exopolysaccharides (25.6 g/L), along (37.9 lipid (4.6 production.

Language: Английский

Citations

0
Closed Fixed-Bed Bacteria-Algae Biofilm Reactor: A Promising Solution for phenol containing wastewater Treatment and Resource Transformation DOI

Yuanyuan Yang,

Siqi Li, Zhou Xin

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 138176 - 138176

Published: April 1, 2025

Language: Английский

Citations

0
Valorization of Algal Biomass to Biofuel: A Review DOI Creative Commons

Vijitha Amalapridman,

Peter Amoako Ofori,

Lord Abbey

et al.

Biomass, Journal Year: 2025, Volume and Issue: 5(2), P. 26 - 26

Published: May 5, 2025

Concerns about sustainable energy sources arise due to the non-renewable nature of petroleum. Escalating demand for fossil fuels and price inflation negatively impact security economy a country. The generation usage biofuel could be suggested as alternative fuels. Several studies have investigated potential using edible crops production. However, algae suitable feedstock is currently being promoted its ability withstand adverse environmental conditions, capacity generate more oil per area, mitigate crises climate change with no detrimental on environment food supply. Furthermore, biorefinery approach in algae-based production controls algal cultivation. Hence, this article critically reviews different cultivation systems critical parameters including harvesting methods, intended biofuels relevant processing techniques, other applications valorized biomass, merits demerits, limitations challenges

Language: Английский

Citations

0
Optimization of Wheat Straw Conversion into Microbial Lipids by Lipomyces tetrasporus DSM 70314 from Bench to Pilot Scale DOI Creative Commons
Antonio Caporusso, Isabella De Bari, Aristide Giuliano

et al.

Fermentation, Journal Year: 2023, Volume and Issue: 9(2), P. 180 - 180

Published: Feb. 16, 2023

Microbial lipids are renewable platforms for several applications including biofuels, green chemicals, and nutraceuticals that can be produced from residual carbon sources. Lignocellulosic biomasses abundant raw materials the production of second-generation sugars with conversion yields depending on quality hydrolysates metabolic efficiency microorganisms. In present work, wheat straw pre-treated by steam explosion enzymatically hydrolysed was converted into microbial Lipomyces tetrasporus DSM 70314. The preliminary optimization enzymatic hydrolysis performed at bench scale through response surface methodology (RSM). fermentation medium set-up were optimized in terms nitrogen (N) source carbon-to-nitrogen (C/N) ratio yielding to selection soy flour as a N C/N 160. settings scaled-up further 10 L-scale finally 50 L pilot bioreactor. Process also included oxygen supply strategies. Under conditions, lipid concentration 14.8 gL−1 achieved corresponding 23.1% w/w yield 67.4% cell content. Oleic acid most fatty percentage 57%. overall process mass balance assessed biodiesel straw.

Language: Английский

Citations

7
Biorefinery of vineyard winter prunings for production of microbial lipids by the oleaginous yeast Cryptococcus curvatus DOI

Xueyuan Zhang,

Zhumei Fang,

Di Zhao

et al.

Waste Management, Journal Year: 2023, Volume and Issue: 168, P. 221 - 229

Published: June 11, 2023

Language: Английский

Citations

6
Microbial composition of spoiled irradiated ready-to-eat chicken feet and their spoilage characteristics DOI
Xin Jia, Dan Wang,

Ailian Meng

et al.

Food Microbiology, Journal Year: 2024, Volume and Issue: 124, P. 104620 - 104620

Published: Aug. 14, 2024

Language: Английский

Citations

2
Diversity analysis and gene function prediction of bacteria and fungi of Bactrian camel milk and naturally fermented camel milk from Alxa in Inner Mongolia DOI Creative Commons
Wuyundalai Bao,

Yuxing He,

Jinghe Yu

et al.

LWT, Journal Year: 2022, Volume and Issue: 169, P. 114001 - 114001

Published: Sept. 20, 2022

The microbial diversity in Bactrian camel milk and naturally fermented with unique nutritional properties medicinal value Alxa, Inner Mongolia, has been little reported. results of this study showed that the dominant bacterial phyla were Firmicutes Proteobacteria, fungal Ascomycota Basidiomycota. During natural fermentation process milk, genus changed from Lactococcus to Lactobacillus, while genera Apiotrichum, Cutaneotrichosporon Candida Kazachstania Kluyveromyces. Therefore, flora structure bacteria fungi significantly different (P < 0.05). predicted gene functions by PICRUSt2 software main metabolic genes global overview maps, carbohydrate metabolism amino acid metabolism, etc.; glyoxylate cycle, aerobic respiration I II, etc. And relative abundance related food-borne pathogenicity environmental pollution such as Enterobacter, Enterococcus Raoultella, suddenly decreased, which provides a reference for safety evaluation products basis probiotics.

Language: Английский

Citations

9