Biotechnology for Biofuels and Bioproducts,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: April 1, 2023
Engineering
sustainable
bioprocesses
that
convert
abundant
waste
into
fuels
is
pivotal
for
efficient
production
of
renewable
energy.
We
previously
engineered
an
Escherichia
coli
strain
optimized
bioethanol
from
lactose-rich
wastewater
like
concentrated
whey
permeate
(CWP),
a
dairy
effluent
obtained
valorization
processes.
Although
attractive
fermentation
performances
were
reached,
significant
improvements
are
required
to
eliminate
recombinant
plasmids,
antibiotic
resistances
and
inducible
promoters,
increase
ethanol
tolerance.
Here,
we
report
new
with
chromosomally
integrated
ethanologenic
pathway
under
the
control
constitutive
promoter,
without
plasmids
resistance
genes.
The
showed
extreme
stability
in
1-month
subculturing,
CWP
similar
plasmid-bearing
strain.
then
investigated
conditions
enabling
sugar
consumption
by
changing
inoculum
size
concentration,
revealing
toxicity-
nutritional-related
bottlenecks.
joint
tolerance,
via
adaptive
evolution,
supplementation
small
ammonium
sulphate
amounts
(0.05%
w/v)
enabled
boost
6.6%
v/v
titer,
1.2
g/L/h
rate,
82.5%
yield,
cell
viability
increased
three
orders
magnitude.
Our
has
features
industrial
settings
represents
relevant
improvement
existing
biotechnologies.
ACS Sustainable Resource Management,
Journal Year:
2025,
Volume and Issue:
2(1), P. 157 - 165
Published: Jan. 3, 2025
Tremendous
quantities
of
textile
waste
generated
and
primarily
landfilled
annually
represent
a
huge
risk
contaminating
the
environment,
together
with
loss
valuable
resources.
Especially,
blended
fabrics
further
pose
challenge
for
recycling
valorization
strategies,
while
enzymatic
hydrolysis
offers
highly
specific
environmentally
friendly
solution.
In
this
study,
we
demonstrate
that
proteases
specifically
hydrolyze
wool
components
in
blends
polyester,
allowing
recovery
pure
polyester
fibers
as
well
amino
acids
peptides
platform
molecules
valorization.
Recovered
were
successfully
used
nitrogen
source
cultivation
Chlorella
vulgaris
Rhodotorula
mucilaginosa
production
biomolecules
including
pigments
lipids.
Here,
11.3
mg/gCDW
chlorophyll
47%
lipid
content
obtained
from
algal
biomass,
1.1
carotenoids
35%
lipids
reached
yeast
grown
on
hydrolysate
sole
source.
These
could
be
applied
natural
dyes
applications
or
biofuels
to
replace
toxic
synthetic
compounds
fossil
resources,
respectively.
The
presented
concept
demonstrates
feasibility
microbial
support
development
toward
circular
bioeconomy.
Energies,
Journal Year:
2022,
Volume and Issue:
15(18), P. 6828 - 6828
Published: Sept. 18, 2022
Agro-industrial
residues
such
as
bagasse,
pomace,
municipal
residues,
vinasse
and
cheese
whey
are
an
environmental
problem
around
the
world,
mainly
due
to
huge
volumes
that
generated
because
of
food
production
satisfy
nutritional
needs
growing
world
population.
Among
above
has
gained
special
attention
its
high
with
a
worldwide
160
million
tons
per
year.
Most
it
is
discarded
in
water
bodies
land
causing
damage
environment
biological
oxygen
demand
caused
by
organic
matter
load.
The
regulations
developing
countries
have
motivated
development
new
processes
treat
transform
into
added-value
products
supplements,
cattle
feed
additives.
In
addition,
during
last
decade,
several
technologies
been
developed
produce
bioenergy
through
biotechnological
process
using
potential
feedstock.
This
review
discusses
bioethanol,
biohydrogen,
biomethane
microbial
lipid-biodiesel
substrate.
Critical Reviews in Food Science and Nutrition,
Journal Year:
2023,
Volume and Issue:
65(5), P. 964 - 991
Published: Dec. 14, 2023
Fermentation
technology
is
a
biorefining
tool
that
has
been
used
in
various
industrial
processes
to
recover
valuable
nutrients
from
different
side
streams.
One
promising
application
of
this
technique
the
reclamation
nutritional
components
seafood
Seafood
processing
generates
significant
amounts
waste,
including
heads,
shells,
and
other
These
streams
contain
high
quantities
valued
can
be
extracted
using
fermentation
technology.
The
engages
microorganisms
convert
stream
into
products
like
biofuels,
enzymes,
animal
feed.
Natural
polymers
such
as
chitin
chitosan
have
purposes
food,
medicinal,
agricultural
industry.
Another
example
fish
protein
hydrolysates
(FPH)
FPHs
are
protein-rich
powders
which
could
nutrition
nutraceutical
resulting
hydrolysate
further
filtered
dried
FPH
powder.
holds
great
possibility
recovery
process
help
reduce
waste
generate
new
value-added
what
would
otherwise
considered
product.
With
research
development,
become
key
Discover Environment,
Journal Year:
2024,
Volume and Issue:
2(1)
Published: May 16, 2024
Abstract
The
surge
in
Nigeria's
population
has
led
to
heightened
demands
for
food,
clean
water,
and
agricultural
products,
resulting
a
significant
increase
waste
generation.
Nigeria
ranks
among
the
top
countries
globally
terms
of
production,
particularly
from
agriculture.
Unfortunately,
management
these
vast
quantities
is
often
inadequate,
leading
environmental
pollution,
disease,
CO
2
emissions.
While
reducing
such
important,
completely
stopping
generation
agriculture
impossible.
Therefore,
it
becomes
imperative
adopt
eco-friendly
cost-effective
approaches
handling
this
waste,
including
collection,
conversion,
treatment,
recycling,
reuse.
Effectively
managing
can
mitigate
pollution
its
adverse
impacts
on
public
health.
This
review
delves
into
environmental,
economic,
health
challenges
associated
with
Nigeria.
It
highlights
importance
implementing
sustainable
practices
reuse,
application.
By
harnessing
materials
effectively,
stands
reap
economic
benefits
while
simultaneously
safeguarding
environment
pollution.
Biotechnology for Biofuels and Bioproducts,
Journal Year:
2023,
Volume and Issue:
16(1)
Published: Feb. 23, 2023
Abstract
Background
Escherichia
coli
cells
are
the
most
frequently
used
hosts
in
recombinant
protein
production
processes
and
mainly
require
molecules
such
as
IPTG
or
pure
lactose
inducers
of
heterologous
expression.
A
possible
way
to
reduce
costs
is
replace
traditional
with
waste
materials
cheese
whey
permeate
(CWP).
CWP
a
secondary
by-product
generated
from
valuable
proteins,
which
obtained
ultrafiltration
whey,
main
dairy
industry,
rich
lactose.
Results
The
effects
collected
an
Italian
plant
were
compared
those
on
two
model
proteins
(i.e.,
green
fluorescent
toxic
Q55
variant
ataxin-3),
E.
BL21
(DE3)
cells.
It
was
found
that
high
content
(165
g/L)
antioxidant
properties
its
micronutrients
(vitamins,
cofactors
osmolytes)
sustain
yields
similar
inducers,
accompanied
by
improvement
cell
fitness.
Conclusions
has
proven
be
effective
low-cost
alternative
inducer
produce
proteins.
Its
use
thus
combines
advantage
exploiting
product
reducing
Journal of Food Processing and Preservation,
Journal Year:
2023,
Volume and Issue:
2023, P. 1 - 24
Published: April 20, 2023
Increased
milk
production
has
boosted
the
market
of
milk-driven
products,
and
as
a
result,
by-product
also
increased,
which
is
challenge
to
dispose
of.
Whey,
cheese
by-product,
increasing
yearly,
its
disposal
in
water
bodies
responsible
for
pollution
thus
an
issue
dairy
sector.
In
this
context,
extensive
research
been
going
on
valorize
create
alternative
ways
remove
organic
load
whey
rather
than
disposing
it.
Recently,
exciting
developments
have
made
convert
into
value-added
commodities
such
biofuels
(bioethanol,
biodiesel,
biohydrogen),
bioplastics,
bacterial
cellulose,
food
colors
flavors,
bioprotective
solutions,
bioactive
peptides,
single-cell
proteins.
review,
we
aim
comprehend
recent
challenges
producing
whole
range
ingredients
with
feedstock
through
microbial
fermentation.
Particular
focus
was
paid
potential
novel
genetically
engineered
or
adapted
strains
bovine
economically
sustainably.
LWT,
Journal Year:
2023,
Volume and Issue:
176, P. 114524 - 114524
Published: Jan. 29, 2023
In
this
work,
three
lactic
acid
bacteria
(LAB)
strains,
specifically,
Lactiplantibacillus
plantarum
UMCC
2996,
Furfurilactobacillus
rossiae
3002,
and
Pediococcus
pentosaceus
3010,
were
tested
in
new
bread-whey
media
composed
by
wheat
bread
sweet
cheese
whey,
designed
as
an
alternative
to
the
conventional
MRS
medium.
The
medium
resulting
from
hydrolysis
with
amylase
neutrase
(AN)
was
considered
best
for
growth
of
all
strains.
This
particularly
optimal
strain
F.
which
showed
increase
114%
compared
that
Additionally,
bio-preservative
ability
selected
LAB
assessed
pectin-based
coated
sliced
inoculated
Aspergillus
flavus
ITEM
7828,
Penicillium
paneum
1381,
niger
7090.
Different
behavior
observed
towards
specific
molds.
A
good
bio-preservation
activity
shown
3002
against
A.
7828
results
protection
ethanol
treatment.
obtained
study
suggest
a
novel
strategy
cultivation
starters
bio-protection
valorizing
waste
whey
by-products,
circular
economy
perspective.
Journal of Flow Chemistry,
Journal Year:
2023,
Volume and Issue:
14(1), P. 85 - 96
Published: Nov. 7, 2023
Abstract
The
number
of
biocatalyzed
reactions
at
industrial
level
is
growing
rapidly
together
with
our
understanding
on
how
we
can
maximize
the
enzyme
efficiency,
stability
and
productivity.
While
biocatalysis
nowadays
recognized
as
a
greener
way
to
operate
in
chemistry,
its
combination
continuous
processes
has
lately
come
up
powerful
tool
enhance
process
selectivity,
productivity
sustainability.
This
perspective
aims
describing
recent
advances
this
technology
future
developments
leading
smart,
efficient
strategies
for
optimization
large-scale
production.
Graphical
Fermentation,
Journal Year:
2024,
Volume and Issue:
10(6), P. 278 - 278
Published: May 24, 2024
The
shift
from
fossil
fuels
to
renewable
energy
sources
is
crucial
in
addressing
environmental
challenges.
Vegetable
oils
have
been
focused
on
as
the
main
potential
source
for
biodiesel
and
biolubricant
production.
However,
due
their
fatty
acid
(FA)
composition
they
are
characterized
by
low
stability
oxidation
variable
viscosity.
Single-cell
(SCOs)
oleaginous
microorganisms
a
possible
alternative
vegetable
oils:
more
suitable,
it
can
further
be
improved
controlling
fermentation’s
physiological
conditions.
In
present
study,
production
of
SCOs
with
targeted
technological
properties
Lipomyces
starkeyi
fermentation
under
controlled
temperatures
was
assessed.
A
dairy
effluent
(scotta)
used
substrate
improve
economic
sustainability
process.
Batch
aerobic
fermentations
were
carried
out
fermenter
at
two
different
(25
°C
30
°C).
yields
SCO
FA
profiles
analyzed.
highest
biomass
(9.76
g
L−1)
microbial
oil
(1.83
obtained
°C.
Furthermore,
significantly
lower
content
(46%
vs.
55%)
unsaturated
FAs
higher
(11%
1.5%)
shorter-chain
saturated
FAs,
myristic
almost
matching
stearic
acid,
detected
comparison
25
Very
peroxide
values
also
found
(0.14
meq
O2
kg−1
0
These
results
indicate
that
these
highly
oxidation-resistant,
temperature
improves
oxidative
tribophysical
features.
biodiesels’
properties,
calculated
composition,
within
limits
both
U.S.
standards
E.U.
regulations.
Then,
produced
L.
effluents
considered
suitable
produce
biolubricants.
