Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(6), P. 114617 - 114617
Published: Oct. 30, 2024
Language: Английский
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142878 - 142878
Published: April 1, 2025
Language: Английский
Citations
2
Reaction Chemistry & Engineering, Journal Year: 2023, Volume and Issue: 8(11), P. 2689 - 2702
Published: Jan. 1, 2023
A review of the literature covering research on immobilization lipases hydrophobic supports was performed using systematic mapping (SM) concepts.
Language: Английский
Citations
21
Industrial & Engineering Chemistry Research, Journal Year: 2023, Volume and Issue: 62(36), P. 14111 - 14129
Published: Aug. 29, 2023
Enzymes have been widely used as biocatalysts to meet the increasing demand for industrial approaches. Even if method of immobilization stabilize enzymes seems easy and effective scientists, maintenance activity remains currently an open challenge in various applications. Activity enhancement has recently gained a huge interest since this is significant point terms In context, external stimulants such ultrasound, magnetic field, electric light irradiation affect enzyme different aspects. Among these, ultrasound induction exhibits prominent role immobilized enzyme. On other hand, although large number articles reported improvements catalytic activities by methods, best our knowledge, review describing these not yet reported. Therefore, novel focused on effects it will provide perspectives recent information development application related research areas. addition, give insight into mechanisms at molecular level response question how methods enhance activity.
Language: Английский
Citations
11
Process Biochemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 1, 2025
Language: Английский
Citations
0
Química Nova, Journal Year: 2023, Volume and Issue: unknown
Published: Jan. 1, 2023
The current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size and spherical in shape. Excellent biocompatibility sodium resulted 90% immobilization yield for due covalent attachment. Soluble immobilized exhibited its pH temperature-optima at 7.0 40 oC, respectively. However, the enzyme attached ACMONPs enhanced activity higher lower temperature ranges, contrast solution. bound displayed greater under effect galactose mediated product inhibition as well reusability experiment. Our indicated 88% lactose hydrolyzed oC compared 71% soluble identical controlled batch reactors. Hence, this nanomatrix can find potential application continuous packed-bed reactors obtaining lactose-free dairy products.
Language: Английский
Citations
1
Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 153, P. 107855 - 107855
Published: Oct. 9, 2024
Rhamnolipids (RLs) are widely studied biosurfactants with significant industrial potential in cosmetics, pharmaceuticals, and bioremediation due to their excellent surface activity, emulsifying properties bioactive characteristics. However, high production costs impede mass production. This study investigates the immobilization of Pseudomonas stutzeri lipase (PSL) on various supports enhance RL synthesis efficiency, focusing yield regioselectivity enzymatic 4-O-lauroylrhamnose by transesterification rhamnose vinyl laurate. Three methods were compared: covalent binding, adsorption Celite, hydrophobic supports. The efficiency varied depending method used, lowest observed for Celite (56 %), followed Sepabeads (EC-EP/S 78 % EC-EP/L 70 highest (83-97 %, EC-OD being best at 97 %). For 4-O-lauroylrhamnose, Sepabeads™ EC-EP yielded low conversions restricted conformational freedom enzyme. Celite® 545 resulted moderate conversion rates, limited electrostatic interactions restricting enzyme activity. most promising results obtained supports, particularly Purolite® ECR8806F, achieving nearly complete maintaining 4-position both THF green solvent 2-methyltetrahydrofuran (2-MeTHF). highlights critical role support hydrophobicity active area immobilized performance. PSL ECR8806F demonstrated sustainable RLs production, showing reusability, stability productivity across multiple reaction cycles. presents a advancement optimizing conditions, facilitating way more cost-effective applications.
Language: Английский
Citations
0
Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(6), P. 114617 - 114617
Published: Oct. 30, 2024
Language: Английский
Citations
0