Foods,
Journal Year:
2023,
Volume and Issue:
12(16), P. 3058 - 3058
Published: Aug. 15, 2023
Scientific
mapping
using
bibliometric
data
network
analysis
was
applied
to
analyze
research
works
related
lipases
and
their
industrial
applications,
evaluating
the
current
state
of
research,
challenges,
opportunities
in
use
these
biocatalysts,
based
on
evaluation
a
large
number
publications
topic,
allowing
comprehensive
systematic
analysis,
which
had
not
yet
been
conducted
relation
studies
specifically
covering
applications.
Thus,
involving
lipase
enzymes
published
from
2018
2022
were
accessed
Web
Science
database.
The
extracted
records
result
terms
bibliographic
compatibility
among
articles,
co-occurrence
keywords,
co-citation
journals
VOSviewer
algorithm
construction
maps.
This
review
357
documents,
including
original
revealed
inspired
by
period,
showing
that
development
together
with
different
areas
knowledge,
presents
good
results
applications
lipases,
due
information
synchronization.
Furthermore,
this
showed
main
challenges
regarding
increased
production
operational
stability;
establishing
well-defined
criteria,
such
as
cultivation
conditions,
activity,
biocatalyst
stability,
type
support
reactor;
thermodynamic
studies;
reuse
cycles;
it
can
assist
defining
goals
for
successful
large-scale
several
points
improvement
future
enzymes.
Process Biochemistry,
Journal Year:
2024,
Volume and Issue:
142, P. 104 - 112
Published: April 14, 2024
The
enzymatic
synthesis
of
xylose
fatty
acid
esters
was
carried
out
in
two
steps:
hydrolysis
degummed
soybean
oil
(DSO)
and
esterification
the
produced
purified
free
acids
(FFA)
with
xylose.
Different
lipases
immobilized
on
a
hydrophobic
support
were
evaluated
DSO
an
experimental
design
used
to
optimize
reaction.
Eversa®
Transform
2.0
(EV-Purolite)
yielded
93%
conversion
after
6
h
using
biocatalyst
load
6.53
wt.%,
water/oil
mass
ratio
5.5
temperature
48.4
°C.
High
operational
stability
for
EV-Purolite
found
sequential
batch
strategy
possibility
concentrating
glycerol.
In
stage,
lipase
from
Thermomyces
lanuginosus
(TLL-Purolite)
B
Candida
antarctica
showed
better
performance
than
porcine
pancreas
EV-Purolite.
About
17%
FFAs
(0.85
molecules
FAA
per
molecule)
consumed
enzyme
5
wt.%
TLL-Purolite
FFA/xylose
molar
5.
Sequential
batches
low
TTL-Purolite
due
desorption
TLL
support.
final
mixture
reaction
medium
containing
emulsifying
properties
similar
those
commercial
surfactant.
International Journal of Biological Macromolecules,
Journal Year:
2024,
Volume and Issue:
275, P. 133555 - 133555
Published: July 1, 2024
Here,
we
report
a
study
of
the
effect
blocking
agent
on
properties
lipase
from
Thermomyces
lanuginosus
(TLL)
immobilized
heterofunctional
support
(Purolite
C18-ethylnediamina
(EDA)-
vinyl
sulfone
(VS)-TLL-blocking
agent)
in
different
reactions.
The
performance
biocatalysts
was
compared
to
those
standard
hydrophobic
C18-TLL)
and
commercial
one
(TLL-IM).
nature
(Cys,
Gly
Asp)
altered
enzyme
features.
TLL-IM
always
gave
comparatively
worse
performance,
with
its
specificity
for
oil
being
very
Purolite
biocatalysts.
Under
optimized
conditions,
C18-TLL
yielded
97
%
hydrolysis
conversion
after
4
h
using
water/waste
cooking
soybean
(WCSO)
mass
ratio
4.3,
biocatalyst
load
6.5
wt%
temperature
44.2
°C
(without
buffer
or
emulsification
agent).
In
esterification
reactions
purified
free
fatty
acids
(FFAs)
obtained
WCSO,
best
TLL
depended
utilized
alcohol:
linear
amyl
alcohol
preferred
by
C18-EDA-VS-TLL-Gly,
while
higher
activity
achieved
utilizing
isoamyl
as
nucleophile
C18-EDA-VS-TLL-Cys,
C18-EDA-VS-TLL-Asp
IM-TLL
catalysts.
All
results
indicate
influence
step
final
Catalysts,
Journal Year:
2025,
Volume and Issue:
15(3), P. 209 - 209
Published: Feb. 22, 2025
The
concept
of
combi-lipases
is
herein
explored
in
the
full
hydrolysis
babassu
oil.
commercially
immobilized
lipases
from
Candida
antarctica
(form
B)
(Novozym®
435),
Rhizomucor
miehei
(Lipozyme®
RM-IM),
and
Thermomyces
lanuginosus
TL-IM)
were
evaluated
as
single
combined
biocatalysts
by
a
mixture
design
with
triangular
surface.
As
result,
after
evaluating
response
desirability
profiling
for
all
biocatalysts,
best
biocatalyst
reaction
was
composed
75%
Lipozyme®
RM-IM,
17%
Novozym®
435,
8%
TL-IM,
reaching
(>99%)
4
h
reaction.
Subsequently,
such
employed
optimization
shorter
time
(3
h).
After
Taguchi
method,
reached
under
optimized
conditions
(9
wt.%
content,
1:2
(oil/water),
40
°C,
180
rpm).
Under
conditions,
maintained
70%
their
initial
activity
10
cycles.
antimicrobial
against
some
most
common
environmental
bacteria
obtained
free
fatty
acids
(FFAs)
also
evaluated.
FFAs
inhibited
more
than
90%
growth
S.
aureus,
E.
coli,
P.
aeruginosus
when
using
mg
FFAs/mL.
Catalysts,
Journal Year:
2025,
Volume and Issue:
15(4), P. 375 - 375
Published: April 11, 2025
Biocatalysis—specifically
the
use
of
immobilized
lipases—has
been
proposed
as
a
greener
alternative
for
ester
production.
Several
critical
challenges,
such
high
cost
biocatalysts,
are
delaying
industrial
implementation
biocatalysis.
Moreover,
short-chain
synthesis,
strong
inhibition/inactivation
potential
acids
and
alcohols
on
lipases
leads
to
long
reaction
cycles
and/or
need
organic
solvents
overcome
limitations
solvent-free
systems
and,
consequently,
decrease
in
product
concentrations.
This
review
presents
an
overview
scientific
developments
enzymatic
compiling
constraints
their
syntheses
from
process
perspective,
including
insights
about
key
performance
indicators
(KPI)
economic
parameters.
