A review on the recent progress, opportunities, and challenges of 4D printing and bioprinting in regenerative medicine

Journal of Biomaterials Science Polymer Edition, Journal Year: 2022, Volume and Issue: 34(1), P. 108 - 146

Published: Aug. 4, 2022

Four-dimensional (4 D) printing is a novel emerging technology, which can be defined as the ability of 3 D printed materials to change their form and functions. The term 'time' added fourth dimension, in respond stimulus after finishing manufacturing process. 4 provides more versatility terms size, shape, structure construct. Complex material programmability, multi-material printing, precise design are essential requirements systems. utilization stimuli-responsive polymers has increasingly taken place cell traction force-dependent methods manual folding, offering advanced technique affect construct's adjusted shape transformation. present review highlights concept responsive bioinks used such water-responsive, pH-responsive, thermo-responsive, light-responsive tissue regeneration. Cell force described well. Finally, this paper aims introduce limitations future trends biomedical applications based on selected key references from last decade.

Language: Английский

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Biomaterial-based physical regulation of macrophage behaviour

Journal of Materials Chemistry B, Journal Year: 2021, Volume and Issue: 9(17), P. 3608 - 3621

Published: Jan. 1, 2021

Macrophages play a critical role in regulating immune reactions induced by implanted biomaterials. They are highly plastic and response to diverse stimuli the microenvironment can exhibit spectrum of phenotypes functions. In addition biochemical signals, physical properties biomaterials becoming increasingly appreciated for their significant impact on macrophage behaviour, underlying mechanisms deserve more in-depth investigations. This review first summarises effects key cues - including stiffness, topography, confinement applied force behaviour. Then, it reviews current knowledge cellular sensing transduction into intracellular signals. Finally, discusses major challenges understanding mechanical regulation that could provide insights biomaterial design.

Language: Английский

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Biophysical Approaches for Applying and Measuring Biological Forces

Advanced Science, Journal Year: 2021, Volume and Issue: 9(5)

Published: Dec. 19, 2021

Over the past decades, increasing evidence has indicated that mechanical loads can regulate morphogenesis, proliferation, migration, and apoptosis of living cells. Investigations how cells sense stimuli or mechanotransduction mechanism is an active field biomaterials biophysics. Gaining a further understanding regulation depicting network inside require advanced experimental techniques new theories. In this review, fundamental principles various approaches have been developed to characterize types magnitudes forces experienced at cellular subcellular levels are summarized. The broad applications these introduced with emphasis on difficulties in implementing special biological systems. advantages disadvantages each technique discussed, which guide readers choose most suitable for their questions. A perspective future directions also provided. It anticipated technical advancement be driving force development mechanobiology.

Language: Английский

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Finite Element Modeling of Cells Adhering to a Substrate: An Overview

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(6), P. 2596 - 2596

Published: March 20, 2024

In tissue formation and regeneration processes, cells often move collectively, maintaining connections through intercellular adhesions. However, the specific roles of cell–substrate cell-to-cell mechanical interactions in regulation collective cell migration are not yet fully understood. Finite element modeling (FEM) may be a way to assess more deeply biological, mechanical, chemical phenomena behind adhesion. FEM is powerful tool widely used simulate described by systems partial differential equations. For example, provides information on stress/strain state adhering substrate, as well its mechanobiological behavior. This review paper, after briefly describing basic principles adhesion, surveys most important studies that have utilized investigate structural response substrate how forces acting adhesive structures affect global

Language: Английский

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Microfluidic platforms for single neuron analysis

Materials Today Bio, Journal Year: 2022, Volume and Issue: 13, P. 100222 - 100222

Published: Jan. 1, 2022

Single-neuron actions are the basis of brain function, as clinical sequelae, neuronal dysfunction or failure for most central nervous system (CNS) diseases and injuries can be identified via tracing single-neurons. The bulk analysis methods tend to miscue critical information by assessing population-averaged outcomes. However, its primary requisite in neuroscience analyze single-neurons understand dynamic interplay neurons their environment. Microfluidic systems enable precise control over nano-to femto-liter volumes adjusting device geometry, surface characteristics, flow-dynamics, thus facilitating a well-defined micro-environment with spatio-temporal single-neuron analysis. microfluidic platform not only offers comprehensive landscape study cell diversity at level transcriptome, genome, and/or epigenome individual cells but also has substantial role deciphering complex dynamics development brain-related disorders. In this review, we highlight recent advances devices analysis, i.e., trapping, dynamics, proteomics, transcriptomics, drug delivery level, single axon guidance, differentiation. Moreover, emphasize limitations future challenges focusing on key performances throughput multiparametric activity platforms.

Language: Английский

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Population distributions of single-cell adhesion parameters during the cell cycle from high-throughput robotic fluidic force microscopy

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: May 11, 2022

Abstract Single-cell adhesion plays an essential role in biological and biomedical sciences, but its precise measurement for a large number of cells is still challenging task. At present, typical force measuring techniques usually offer low throughput, few per day, therefore are unable to uncover phenomena emerging at the population level. In this work, robotic fluidic microscopy (FluidFM) was utilized measure parameters high-throughput manner study their distributions in-depth. The investigated cell type genetically engineered HeLa Fucci construct with cycle-dependent expression fluorescent proteins. This feature, combined made it possible first time characterize single-cell various stages cycle. It found that such as energy follow lognormal distribution. Therefore, conclusions based on data or treating normally distributed can be misleading. Moreover, we area significantly smallest, normalized maximal largest colorless (the mitotic (M) early G1 phases). Notably, parameter characterizing elongation until maximum level between substratum also dependent cycle, which quantity smallest cells. A novel parameter, named spring coefficient cell, introduced fraction during mechanical detachment, Cells M phase adhere atypical way, so-called reticular adhesions, different from canonical focal adhesions. We revealed exert higher unit than stiffer. consequences these findings were discussed, together practical relevance observed population-level phenomena.

Language: Английский

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Nanomechanical Characterization of Soft Nanomaterial Using Atomic Force Microscopy

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101506 - 101506

Published: Jan. 1, 2025

Language: Английский

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Role of Cell Adhesion in Cancer Metastasis Formation: A Review

ACS Omega, Journal Year: 2025, Volume and Issue: 10(6), P. 5193 - 5213

Published: Feb. 9, 2025

Intercellular adhesion is accompanied by several physical quantities and actions. In this review, we tried to collect information about the influence of surface energy its impact on cell-cell adhesion. It still undergoes development for cancer treatment. Data receptor-ligand interactions that occur circulating tumor cells (CTCs) are described, receptors as therapeutic targets collected. Additionally, roughness between CTC was monitored. The effects different cell molecules (CAMs) adhesion, growth, proliferation were investigated. This review offers general principles through blockade with blocking drugs inhibitors like computational models describe process Some theoretical based minimum total free interaction CAMs selected organic have been presented. final aim find how modulation CTCs (by medicals or physically) inhibits metastases formation.

Language: Английский

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High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell Level

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Cell-cell binding, mediated by the physical interactions of receptors and their ligands, plays a fundamental role in immune processes such as surveillance T-cell activation. However, current approaches for measuring cell avidity often lack either throughput or quantitative precision. Here, we introduce high-throughput approach quantifying binding lifetimes strength using centrifuge force microscope (CFM)-a compact operating within standard benchtop centrifuge. The CFM enables live monitoring single-cell under force, conducting thousands experiments parallel. To facilitate real-time study interactions, developed next-generation with multichannel fluorescence imaging capabilities. This system accommodates measurements two modes: cell-protein cell-cell assays. Using this system, investigated immune-cell Bispecific Engager (BiTE) molecules, novel immunotherapy designed to enhance targeting cancer cells. In assays, quantified T- B-cell unbinding from BiTE-functionalized surfaces, revealing receptor-specific relationships between ligand concentration strength. examined BiTE-mediated T-cells Nalm6 B-cells, precursor leukemia line, uncovering strong, time-dependent increase avidity. By integrating analysis, provides new insights into dynamic nature immunological broad implications cellular mechanics.

Language: Английский

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Targeted Drug Delivery System for Pulmonary Fibrosis: Design and Development of Biomaterials

BIO Integration, Journal Year: 2025, Volume and Issue: 6(1)

Published: Jan. 1, 2025

Pulmonary fibrosis (PF) is a progressive interstitial lung disease characterized by excessive extracellular matrix deposition and tissue scarring, leading to impaired function respiratory failure. Although current treatments, such as pirfenidone nintedanib, slow progression, they fail completely halt or reverse fibrosis. Therefore, innovative therapeutic strategies are needed. Targeted drug delivery systems (TDDSs) emerging promising solutions. Biomaterials play critical roles in these enhancing specificity, availability, efficacy, while minimizing systemic toxicity. The most notable biomaterials include nanotechnology-based systems, including liposomes polymeric nanoparticles, which facilitate penetration release fibrotic tissues. Hydrogels have three-dimensional structures providing controlled sustained at inflammation sites, therefore particularly valuable PF treatment. Furthermore, biological carriers stem cells vesicles biocompatibility anti-inflammatory effects that improve outcomes. Despite the potential of clinical translation hindered several challenges, immune clearance, stability platforms, optimization retention within diseased Interdisciplinary approaches integrating precision medicine with advancements may provide solutions opening new avenues for This review discusses developments targeted PF, emphasizing importance biomaterials, mechanisms barriers involved pulmonary delivery, future perspectives overcoming limitations. ultimate goal patient outcomes revolutionizing approach treatment through advanced technologies.

Language: Английский

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