Functional Properties and Antimicrobial Activity from Lactic Acid Bacteria as Resources to Improve the Health and Welfare of Honey Bees

Insects, Journal Year: 2022, Volume and Issue: 13(3), P. 308 - 308

Published: March 21, 2022

Honey bees (Apis mellifera) are agriculturally important pollinators. Over the past decades, significant losses of wild and domestic have been reported in many parts world. Several biotic abiotic factors, such as change land use over time, intensive management, pesticides, climate change, beekeeper’s management practices, lack forage (nectar pollen), infection by parasites pathogens, negatively affect honey bee’s well-being survival. The gut microbiota is for bee growth development, immune function, protection against pathogen invasion; moreover, a well-balanced fundamental to support health vigor. In fact, structure intestinal bacterial community can become an indicator status. Lactic acid bacteria normal inhabitants gastrointestinal tract insects, their presence has consistently literature. first section this review, recent scientific advances LABs probiotic supplements diet summarized discussed. second discusses some mechanisms which carry out antimicrobial activity pathogens. Afterward, individual paragraphs dedicated Chalkbrood, American foulbrood, European Nosemosis, Varroosis well potentiality biological control.

Language: Английский

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Prospects of probiotics in beekeeping: a review for sustainable approach to boost honeybee health

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 4, 2024

Language: Английский

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Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey – A review

Food Chemistry X, Journal Year: 2024, Volume and Issue: 23, P. 101524 - 101524

Published: June 5, 2024

Honey is a natural product used since ancient times due to its taste, aroma, and therapeutic properties (antibacterial, antiviral, anti-inflammatory, antioxidant activity). The purpose of this review present the species microorganisms that can survive in honey effect they have on bees consumers. techniques for identifying are also described study. contains bacteria, yeasts, molds, viruses, some them may beneficial humans. antimicrobial acidity high viscosity, sugar concentration, low water content, presence hydrogen peroxide non-peroxidase components, particularly methylglyoxal (MGO), phenolic acids, flavonoids, proteins, peptides, glycopeptides. has antibacterial action (it effectiveness against e.g. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter, etc.), antifungal (effectiveness Candida spp., Aspergillus Fusarium Rhizopus Penicillium spp.), antiviral SARS-CoV-2, Herpes simplex virus type 1, Influenza A B, Varicella zoster virus), antiparasitic Plasmodium berghei, Giardia Trichomonas, Toxoplasma gondii) demonstrated by numerous studies comprised discussed review.

Language: Английский

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Isolation and probiotic evaluation of Apilactobacillus kunkeei and Bombella sp. from Apis mellifera anatoliaca and Bombus terrestris

International Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Language: Английский

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Effects of Insecticides and Microbiological Contaminants on Apis mellifera Health

Molecules, Journal Year: 2021, Volume and Issue: 26(16), P. 5080 - 5080

Published: Aug. 22, 2021

Over the past two decades, there has been an alarming decline in number of honey bee colonies. This phenomenon is called Colony Collapse Disorder (CCD). Bee products play a significant role human life and have huge impact on agriculture, therefore bees are economically important species. Honey found its healing application various sectors life, as well other such royal jelly, propolis, pollen. There many putative factors CCD, air pollution, GMO, viruses, or predators (such wasps hornets). It is, however, believed that pesticides microorganisms mass extinction Insecticides chemicals dangerous to both humans environment. They can cause enormous damage bees’ nervous system permanently weaken their immune system, making them vulnerable factors. Some insecticides negatively affect are, for example, neonicotinoids, coumaphos, chlorpyrifos. Microorganisms diseases bees, weakening health colony often resulting extinction. Infection with may result need dispose entire hive prevent spread pathogens hives. Many aspects still unclear. The deepen knowledge this matter crucial, bearing mind how these animals life.

Language: Английский

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Chemical Profiling and Nutritional Evaluation of Bee Pollen, Bee Bread, and Royal Jelly and Their Role in Functional Fermented Dairy Products

Molecules, Journal Year: 2022, Volume and Issue: 28(1), P. 227 - 227

Published: Dec. 27, 2022

Honeybee products, as multicomponent substances, have been a focus of great interest. The present work aimed to perform the nutritional and chemical profiling biochemical characterization bee pollen (BP), bread (BB), royal jelly (RJ) study their applications in fortification functional fermented dairy products. Their effects on starter cultures physicochemical sensorial quality products were monitored. A molecular networking analysis identified total 46 compounds three that could be potential medicines, including flavonoids, fatty acids, peptides. BB showed highest protein sugar contents (22.57 26.78 g/100 g), which cover 45.14 53.56% daily values (DVs), with considerable amounts essential amino acids threonine lysine (59.50 42.03%). BP, BB, RJ can considered sources iron, 100 g 141, 198.5, 94.94% DV%, respectively. BP was revealed phenolic flavonoid (105.68 43.91 µg/g) synergetic effect when mixed RJ, resulting increased antioxidant activity, while terms both proteolytic powers (IC

Language: Английский

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Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research

Insect Science, Journal Year: 2022, Volume and Issue: 29(4), P. 958 - 976

Published: May 14, 2022

Abstract Eusocial bumble and honey bees are important pollinators for global ecology the agricultural economy. Although both possess similar host‐restricted gut microbiota, they differ in aspects of morphology, autonomy, physiology, behavior, life cycle. The social bee bacteria exhibit host specificity that is likely a result long‐term co‐evolution. unique cycle bumblebees key acquisition development their affects strain‐level diversity core bacterial species. Studies on bumblebee show retain less functional capacity carbohydrate metabolism compared with honeybee. We discuss potential roles microbiota against pathogenic threats application host‐specific probiotics bumblebees. Given advantages microbiome, including simple structure specificity, ease manipulating colonies, we propose may provide valuable system understanding general principles host–microbe interactions, gut–brain axis, vertical transmission.

Language: Английский

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Beneficial bacteria as biocontrol agents for American foulbrood disease in honey bees (Apis mellifera)

Journal of Insect Science, Journal Year: 2023, Volume and Issue: 23(2)

Published: March 1, 2023

Abstract American foulbrood (AFB) is a cosmopolitan bacterial disease that affects honey bee (Apis mellifera) larvae and causes great economic losses in apiculture. Currently, no satisfactory methods are available for AFB treatment mainly due to the difficulties eradicate tenacious spores produced by etiological agent of AFB, Paenibacillus (Bacillales, Paenibacillaceae). This present review focused on beneficial bacteria displayed antagonistic activities against P. demonstrated potential control. Emphases were placed commensal (genus Bacillus lactic acid particular) alimentary tract bees. The probiotic roles play combating pathogenic limitations referring application these addressed.

Language: Английский

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Lactobacillus huangpiensis sp. nov. and Lactobacillus laiwuensis sp. nov., isolated from the gut of honeybee (Apis mellifera)

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2022, Volume and Issue: 72(2)

Published: Feb. 10, 2022

Four Gram-stain-positive bacterial strains were isolated from the gut of honeybee (Apis mellifera) in China. These characterized using a polyphasic taxonomic approach. The data demonstrated that three four represented two novel species genus Lactobacillus, F306-1T and F551-2T designated as type strains. Results 16S rRNA gene sequence analysis indicated F306-1T, F447 phylogenetically related to Lactobacillus kimbladii kullabergensis, having 99.1-99.7 % (about 1400 bp) similarities. phylogenetic tree based on concatenated pheS, rpoA, gyrB, hsp60, recA, rpoB tuf sequences (4114 phylogenomic whole genome most closely L. kullabergensis Biut2NT, strain was Hma2NT. Strains shared 99.9 average nucleotide identity (ANI), 99.7 digital DNA-DNA hybridization (dDDH) amino acid (AAI) values, indicating they belong same species. Strain exhibited highest ANI (94.4 %), dDDH (56.7 %) AAI (94.7 values Biut2NT. had (94.0 (54.3 (95.8 with Acid production amygdalin, maltose, starch, gentiobiose turanose, activity esterase (C4) α-glucosidase, growth 3 NaCl at 37 °C under strict anaerobic condition (on mMRS agar plates), 1-6% aerobic plates supplemented 0.05 cysteine or 1 2 fructose) could differentiate DSM 26262T. d-glucose, arbutin gentiobiose, 45 plates) 26263T. Based upon obtained present study, species, huangpiensis sp. nov. laiwuensis nov., are proposed (=LMG 32144T=JCM 34361T=CCTCC AB 2020300T) (=JCM 34502T=CCTCC 2021027T), respectively.

Language: Английский

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Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Animals, Journal Year: 2023, Volume and Issue: 13(6), P. 1059 - 1059

Published: March 15, 2023

Lactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, probiotic potential 10 LAB strains was determined, which in our previous studies showed most potent protective abilities. In current study, we investigated various properties candidates. The tested varied susceptibility to antibiotics. Isolates high sugar syrups gastrointestinal conditions. None exhibited β-hemolytic activity, mutual antagonism, mucin degradation, hydrogen peroxide production capacity, or bile salt hydrolase (BSH) activity. Additionally, cytotoxicity cell-free supernatants (CFS) assessed, as well effect CFS from P. pentosaceus 14/1 on coumaphos chlorpyrifos Caco-2 cell line. cells reached up 89.81% presence highest concentration CFS. Furthermore, metabolites decreased insecticides (up 19.32%) thus demonstrating cytoprotective All produced lactic, acetic, malonic acids. This research allowed selection effective strains, terms probiosis, future vivo aimed at developing an ecologically biopreparation

Language: Английский

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