A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise DOI Creative Commons
Seong Eun Kwak, Amy Zheng, Edward B. Arias

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(1), P. e0295964 - e0295964

Published: Jan. 30, 2024

Some acute exercise effects are influenced by postexercise (PEX) diet, and these diet-effects attributed to differential glycogen resynthesis. However, this idea is challenging test rigorously. Therefore, we devised a novel genetic model modify muscle synthase 1 (GS1) expression in rat skeletal with an adeno-associated virus (AAV) short hairpin RNA knockdown vector targeting GS1 (shRNA-GS1). Contralateral muscles were injected scrambled shRNA (shRNA-Scr). Muscles from exercised (2-hour-swim) time-matched sedentary (Sed) rats collected immediately (IPEX), 5-hours-PEX (5hPEX), or 9-hours-PEX (9hPEX). Rats 5hPEX 9hPEX experiments refed (RF) not-refed (NRF) chow. analyzed for glycogen, abundance of metabolic proteins (pyruvate dehydrogenase kinase 4, PDK4; peroxisome proliferator-activated receptor γ coactivator-1α, PGC1α; hexokinase II, HKII; glucose transporter GLUT4), AMP-activated protein phosphorylation (pAMPK), metabolism-related enzymes (glycogen phosphorylase, PYGM; debranching enzyme, AGL; branching GBE1). shRNA-GS1 versus paired shRNA-Scr had markedly lower abundance. IPEX Sed greater pAMPK, neither IPEX-values differed muscles. groups did not differ proteins, regardless knockdown. Glycogen RF-rats was at both 9hPEX. HKII groups, despite differing levels. At 9hPEX, PDK4 PGC1α within each diet group. the magnitude changes similar group although between only RF-rats. In summary, established approach investigate relationship other effects. Our results suggest that exercise-effects on several uniformly correspond differences glycogen.

Language: Английский

Relationship of Low Vitamin B6 Status with Sarcopenia, Frailty, and Mortality: A Narrative Review DOI Open Access
Norihisa Katô, Akiko Kimoto,

Peipei Zhang

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(1), P. 177 - 177

Published: Jan. 4, 2024

Marginal vitamin B6 (B6) deficiency is a widespread global concern. Inadequate levels have been linked to an increased risk of age-related chronic diseases such as cardiovascular and cancers. In recent years, the growing concern over sarcopenia (the loss muscle mass strength) frailty (a decline in physiological resilience vulnerability associated with aging) particularly relevant due emergence super-aged societies developed countries. Notably, among thirty-one studies included this review, twenty-five showed significant association status sarcopenia, frailty, all-cause mortality adults (p < 0.05), while six no association. Emerging suggested novel mechanisms underlying These involve P2X7 receptor-mediated NLRP3 inflammasome signaling, AMPK PD-L1 satellite cell-mediated myogenesis. Furthermore, modulation PLP-dependent enzymes impaired metabolic processes, affecting energy utilization, imidazole peptide production, hydrogen sulfide well kynurenine pathway, all which play vital roles skeletal health pathophysiology. This narrative review provides up-to-date assessment our current understanding potential role nutritional combating mortality.

Language: Английский

Citations

16
Emerging biomarkers for the detection of cardiovascular diseases DOI Creative Commons
Sreenu Thupakula, Shiva Shankar Reddy Nimmala,

Haritha Ravula

et al.

The Egyptian Heart Journal, Journal Year: 2022, Volume and Issue: 74(1)

Published: Oct. 20, 2022

The prevalence of cardiovascular disease (CVD) has been continuously increasing, and this trend is projected to continue. CVD rapidly becoming a significant public health issue. Every year there spike in hospital cases CVD, critical concern lower- middle-income countries. Based on identification novel biomarkers, it would be necessary study evaluate the diagnostic requirements or expedite early detection.The literature review was written using wide range sources, such as well-known medical journals, electronic databases, manuscripts, texts, other writings from university library. After that, we analysed specific markers compiled systematic review. A growing body clinical research aims identify people who are at risk for by looking biomolecules. small number biomarkers have shown useful reliable medicine. Biomarkers can used variety applications, predicting heart risk, diagnosing disease, outcomes. As result ability single molecule act biomarker, its usefulness medicine expected increase significantly.Based assessing current trends application markers, discussed described coronary cerebrovascular rheumatic illnesses. Furthermore, focuses procedures that should considered establish comprehensive nature expression illness.

Language: Английский

Citations

42
Electron transfer mechanism of intracellular carbon-dependent DNRA inside anammox bacteria DOI
Chao Wang, Sen Qiao

Water Research, Journal Year: 2023, Volume and Issue: 244, P. 120443 - 120443

Published: Aug. 3, 2023

Language: Английский

Citations

32
Dissimilatory Nitrate Reduction to Ammonia in the natural environment and wastewater treatment facilities: A comprehensive review DOI Creative Commons
Long Zhao,

Jinyu Chen,

Gongqi Shen

et al.

Environmental Technology & Innovation, Journal Year: 2025, Volume and Issue: unknown, P. 104011 - 104011

Published: Jan. 1, 2025

Language: Английский

Citations

1
Circular RNA circZFPM2 regulates cardiomyocyte hypertrophy and survival DOI Creative Commons

Dimyana Neufeldt,

Arne Schmidt,

Elisa Mohr

et al.

Basic Research in Cardiology, Journal Year: 2024, Volume and Issue: 119(4), P. 613 - 632

Published: April 19, 2024

Hypertrophic cardiomyopathy (HCM) constitutes the most common genetic cardiac disorder. However, current pharmacotherapeutics are mainly symptomatic and only partially address underlying molecular mechanisms. Circular RNAs (circRNAs) a recently discovered class of non-coding emerged as specific powerful regulators cellular functions. By performing global circRNA-specific next generation sequencing in tissue patients with hypertrophic compared to healthy donors, we identified circZFPM2 (hsa_circ_0003380). CircZFPM2, which derives from ZFPM2 gene locus, is highly conserved regulatory circRNA that strongly induced HCM tissue. In vitro loss-of-function experiments were performed neonatal rat cardiomyocytes, human pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), HCM-patient-derived hiPSC-CMs. A knockdown was found induce cardiomyocyte hypertrophy compromise mitochondrial respiration, leading an increased production reactive oxygen species apoptosis. contrast, delivery recombinant circZFPM2, packaged lipid-nanoparticles or using AAV-based overexpression, rescued expression promoted cell survival. Additionally, HCM-derived organoids exhibited improved contractility upon CM-specific overexpression circZFPM2. Multi-Omics analysis further our hypothesis, showing beneficial effects on function. Collectively, data highlight serves promising target for treatment including HCM.

Language: Английский

Citations

6
Genome-wide association study identified candidate SNPs and genes associated with hypoxia tolerance in large yellow croaker (Larimichthys crocea) DOI
Jie Ding, Yibo Zhang, Jiaying Wang

et al.

Aquaculture, Journal Year: 2022, Volume and Issue: 560, P. 738472 - 738472

Published: June 8, 2022

Language: Английский

Citations

22
Mechanisms of nutrient absorption and transport of digestion and fermentation metabolites in fish balls with red algae polysaccharides DOI
Liyuan Wang, Yujia Ou,

Menghan Ma

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105833 - 105833

Published: Jan. 1, 2025

Language: Английский

Citations

0
D-glyceric acid enhances the maintenance of glycerophospholipid membranes in 50-60-year-old healthy humans DOI Creative Commons
Olli Petteri Hirvonen, Heikki Kainulainen

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Abstract Background Already 4-day D-glyceric acid (DGA) regimen causes strong positive metabolic effects in older humans that persist even after 2-week follow-up period. Moreover, the resemble exercise and especially membrane integrity seems to be enhanced. Purpose We aim further elucidate both enhancement of exogenous DGA. Methods investigate responses underlying flows indirectly. use nonacute measurements blood metabolites intracellular mRNA expressions, which cover all essential steps studied pathway. Strictly irreversible enzyme reactions, their changes associations are gears. There were altogether 5 different observation points with control study. During first week, was division participants into high lower aerobic capacity subgroups perceived placebo group. Thereafter, genuine Results De novo synthesis DGA mostly from glycerol. GLYCTK activation coincides increased lipids. Lipids obtained TGs lipase reactions simultaneously liberate Endogenous glycerol prolongs effect Phosphorylated (2PG) is used as a carbon backbone glycerophospholipid (GPL) via glyceroneogenesis. we showed de GPL during regimen, furthermore led significant reduction systemic inflammation. Additionally, our results suggest lactate ATP acutely generated each dose glycogen. Conclusions generation physical likely explains exercise-like Now elucidated DGA-GPL-pathway underlines potential an effective medicine for age-associated degeneration diseases unmet medical need such neurodegeneration. Trial registration number (14th Jan 2021), ClinicalTrials.gov Identifier: NCT04713319

Language: Английский

Citations

0
The Swimming Performance and Transcriptomic Insights Into Diverse Gene Regulation in Grass Carp Brain Under Water Velocity Stress DOI Creative Commons
Mian Adnan Kakakhel, Nishita Narwal, Alam Khan

et al.

Water Resources Research, Journal Year: 2025, Volume and Issue: 61(3)

Published: March 1, 2025

Abstract By linking gene regulation to swimming performance under different water flow conditions, the study could reveal how fish adapt their environments, providing insights into evolutionary biology and ecology. The current observed significant variations in various velocities examined associated regulation. Grass carp were subjected controlled measure critical speed ( U crit ), which showed that was increased based on body length; however, a reduction as p < 0.05). Additionally, brain samples collected for transcriptomic analysis, revealed differentially expressed genes (DEGs) functionally annotated revealing key pathways with changed behavior patterns. Enrichment analysis variation all groups including 0.05***), skeletal system development hormone activity muscle contraction 0.05**), locomotion 0.05*), swim bladder 0.05*) found major regulators of grass velocities. Moreover, some identified significantly enzymes hormones, play potential role during such gene‐ca7 0.005***). provides evidence neurogenetic mechanism underlying velocity, have important implications understanding impact hydrodynamics fish.

Language: Английский

Citations

0
Phyto-synthesized silver nanoparticles from Sargassum subrepandum: anticancer, antimicrobial, and molluscicidal activities DOI Creative Commons
Heba El-Sayed, Asmaa Abdelsalam, Mostafa Y. Morad

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 8, 2024

In the realm of nanotechnology, use algae to produce nanoparticles is an environmentally friendly, sustainable, and economically viable strategy. present study, brown macroalgae Sargassum subrepandum was utilized effectively silver (AgNPs). Through various characterization techniques, AgNPs’ structural integrity confirmed. AgNPs exhibited significant antimicrobial activity against Pseudomonas aeruginosa Fusarium equiseti . showed cytotoxic effects on MCF-7 breast adenocarcinoma cell line with IC 50 12.5 µg/ml. Treatment resulted in a marked reduction viability, alongside evident apoptotic necrotic morphological changes cancer cells. molecular docking studies, deeper understanding interaction between crucial proteins related has been achieved, promising molluscicidal action Biomphalaria alexandrina snails, Schistosoma mansoni intermediate host. The half-lethal dose (LC ) determined be 0.84 mg/L. potential consequences its administration include disruptions glycolysis profile, as well impacts steroidal hormone’s estrogen testosterone certain kidney function tests. This study highlights diverse uses algae-synthesized AgNPs, ranging from healthcare environmental management, demonstrating their importance advancing nano-biotechnological solutions.

Language: Английский

Citations

3