The Drosophila drop-dead gene is required for eggshell integrity DOI Creative Commons
Tayler D. Sheahan,

Amanpreet Grewal,

Laura E. Korthauer

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(12), P. e0295412 - e0295412

Published: Dec. 5, 2023

The eggshell of the fruit fly Drosophila melanogaster is a useful model for understanding synthesis complex extracellular matrix. synthesized during mid-to-late oogenesis by somatic follicle cells that surround developing oocyte. We previously reported female flies mutant gene drop-dead (drd) are sterile, but underlying cause sterility remained unknown. In this study, we examined role drd in synthesis. show eggs laid females fertilized arrest early embryogenesis, and innermost layer eggshell, vitelline membrane, abnormally permeable to dye these eggs. addition, major membrane proteins fail become crosslinked nonreducible bonds, process normally occurs egg activation following ovulation, as evidenced their solubility detection Western blot contrast, Cp36 protein, which found outer chorion layers becomes normally. To link expression pattern with phenotypes, expressed ovarian beginning mid-oogenesis, and, importantly, all phenotypes could be recapitulated selective knockdown cells. determine whether was required crosslinking itself, performed vitro experiments. membranes control chambers either incubation hyperosmotic medium, activates chambers, or exogenous peroxidase hydrogen peroxide. neither treatment resulted chambers. These results indicate necessary serve substrates peroxidase-mediated cross-linking at end oogenesis.

Language: Английский

Mouse oocytes develop in cysts with the help of nurse cells DOI Creative Commons
Wanbao Niu, Allan C. Spradling

Cell, Journal Year: 2022, Volume and Issue: 185(14), P. 2576 - 2590.e12

Published: May 26, 2022

Mouse germline cysts, on average, develop into six oocytes supported by 24 nurse cells that transfer cytoplasm and organelles to generate a Balbiani body. We showed between E14.5 P5, cysts periodically activate some begin cytoplasmic transfer, which causes them shrink turnover within 2 days. Nurse die programmed cell death (PCD) pathway involving acidification, similar Drosophila cells, only infrequently apoptosis. Prior initiating co-cluster scRNA-seq with their pro-oocyte sisters, but during final days, they cluster separately. The genes promoting oocyte development PCD are upregulated, whereas the repress such as Tex14, factors, Nobox Lhx8, under-expressed. transferred centrosomes build cytocentrum establishes large microtubule aster in primordial organizes body, defining earliest polarity.

Language: Английский

Citations

69
Conservation of oocyte development in germline cysts from Drosophila to mouse DOI Creative Commons
Allan C. Spradling, Wanbao Niu, Qi Yin

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Nov. 29, 2022

Recent studies show that pre-follicular mouse oogenesis takes place in germline cysts, highly conserved groups of oogonial cells connected by intercellular bridges develop as nurse well an oocyte. Long studied Drosophila and insect gametogenesis, female cysts acquire cytoskeletal polarity traffic centrosomes organelles between the oocyte to form Balbiani body, a marker polarity. Mouse development cell dumping are supported dynamic, cell-specific programs gene expression. High levels perinatal germ death this species primarily result from programmed turnover after transfer rather than defective production. The striking evolutionary conservation early mechanisms distant animal strongly suggests gametogenesis embryonic vertebrates invertebrates share even more common currently believed.

Language: Английский

Citations

44
An evolutionary mechanism to assimilate new nutrient sensors into the mTORC1 pathway DOI Creative Commons
Grace Y. Liu, Patrick Jouandin,

Raymond E. Bahng

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 21, 2024

Abstract Animals sense and respond to nutrient availability in their environments, a task coordinated part by the mTOR complex 1 (mTORC1) pathway. mTORC1 regulates growth response nutrients and, mammals, senses specific amino acids through specialized sensors that bind GATOR1/2 signaling hub. Given animals can occupy diverse niches, we hypothesized pathway might evolve distinct different metazoan phyla. Whether such customization occurs, how capture new inputs, is unknown. Here, identify Drosophila melanogaster protein Unmet expectations (CG11596) as species-restricted methionine sensor directly binds fly GATOR2 fashion antagonized S-adenosylmethionine (SAM). We find Dipterans rapidly evolved capacity thereby repurpose previously independent methyltransferase SAM sensor. Thus, modular architecture of allows it co-opt preexisting enzymes expand its sensing capabilities, revealing mechanism for conferring evolvability on an otherwise conserved system.

Language: Английский

Citations

10
Hyperactive Rac stimulates cannibalism of living target cells and enhances CAR-M-mediated cancer cell killing DOI Creative Commons
Abhinava K. Mishra, Melanie M. Domenech Rodríguez, Alba Yurani Torres

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(52)

Published: Dec. 18, 2023

The 21kD GTPase Rac is an evolutionarily ancient regulator of cell shape and behavior. Rac2 predominantly expressed in hematopoietic cells where it essential for survival motility. hyperactivating mutation E62K also causes human immunodeficiency, although the mechanism remains unexplained. Here, we report that Drosophila, stimulates ovarian to cannibalize neighboring cells, destroying tissue. We then show hyperactive HL60-derived macrophage-like engulf kill living T leukemia cells. Primary mouse +/E62K bone-marrow-derived macrophages primary due a combination macrophage hyperactivity hypersensitivity engulfment. Additionally, non-autonomously stimulate wild-type enhances engulfment target cancer by chimeric antigen receptor-expressing (CAR-M) CAR-dependent manner. propose Rac-mediated cannibalism may contribute immunodeficiency enhance CAR-M immunotherapy.

Language: Английский

Citations

15
Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells DOI Creative Commons
Liang-Yu Pang, Steven DeLuca, Haolong Zhu

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Aug. 31, 2023

Highly potent animal stem cells either self renew or launch complex differentiation programs, using mechanisms that are only partly understood. Drosophila female germline (GSCs) perpetuate without change over evolutionary time and generate cystoblast daughters develop into nurse oocytes. Cystoblasts initiate by generating a transient syncytial state, the cyst, increasing pericentromeric H3K9me3 modification, actions likely to suppress transposable element activity. Relatively open GSC chromatin is further restricted Polycomb repression of testis somatic cell-expressed genes briefly active in early germ cells. Subsequently, Neijre/CBP Myc help upregulate growth reprogram metabolism altering mitochondrial transmembrane transport, gluconeogenesis, other processes. In all these respects resembles development totipotent zygote. We propose cell state was shaped need resist transposon activity timescales.

Language: Английский

Citations

12
Finishing the egg DOI
Celeste A. Berg, Matthew Sieber, Jianjun Sun

et al.

Genetics, Journal Year: 2023, Volume and Issue: 226(1)

Published: Nov. 22, 2023

Gamete development is a fundamental process that highly conserved from early eukaryotes to mammals. As germ cells develop, they must coordinate dynamic series of cellular processes support growth, cell specification, patterning, the loading maternal factors (RNAs, proteins, and nutrients), differentiation structures enable fertilization ensure embryonic survival, other make functional oocyte. To achieve these goals, integrate complex milieu environmental developmental signals produce fertilizable eggs. Over past 50 years, Drosophila oogenesis has risen forefront as system interrogate sophisticated mechanisms drive oocyte development. Studies in have defined control meiosis, protect genome integrity, facilitate mRNA trafficking, nutrients. Work this provided key insights into establish egg chamber polarity patterning well ovulation activation. Using power genetics, field begun define molecular stresses nutrient availability with Importantly, majority reproductive are throughout evolution, many play critical roles somatic tissues well. In chapter, we summarize recent progress several areas impact ovulation. First, discuss storage trafficking during maturation vitellogenesis. Second, examine regulate follicle how impacts construction shell establishment polarity. Finally, regulatory ovulation, activation, successful fertilization.

Language: Английский

Citations

10
Multi-modal comparison of molecular programs driving nurse cell death and clearance in Drosophila melanogaster oogenesis DOI Creative Commons
Shruthi Bandyadka,

Diane Patricia Vig Lebo,

Albert A. Mondragon

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(1), P. e1011220 - e1011220

Published: Jan. 3, 2025

The death and clearance of nurse cells is a consequential milestone in Drosophila melanogaster oogenesis. In preparation for oviposition, the germline-derived bequeath to developing oocyte all their cytoplasmic contents undergo programmed cell death. controlled non-autonomously precipitated by epithelial follicle somatic origin acquiring squamous morphology acidifying externally. Alternatively, stressors such as starvation can induce earlier mid-oogenesis, manifesting apoptosis signatures, followed engulfment cells. To identify contrast molecular pathways underlying these morphologically genetically distinct paradigms, both mediated cells, we compared genome-wide transcriptional, translational, secretion profiles before after differentiating acquire phagocytic capability, well during well-fed nutrient-deprived conditions. By coupling GAL4-UAS system Translating Ribosome Affinity Purification (TRAP-seq) proximity labeling (HRP-KDEL) Liquid Chromatography tandem mass-spectrometry, performed high-throughput screens selectively activated or repressed employ cell-clearance routines. We also integrated two publicly available single-cell RNAseq atlases ovary define transcriptomic this report, describe genes major identified striking consequences oogenesis caused RNAi perturbation prioritized candidates. our knowledge, study first its kind comprehensively characterize apoptotic non-apoptotic paradigms same multi-cellular system. Beyond differences death, investigation may provide insights into how key systemic trade-offs are made between survival reproduction when faced with physiological stress.

Language: Английский

Citations

0
Ovaries of Lumbricidae earthworms (Annelida, Crassiclitellata), from morphology to ultrastructure DOI Creative Commons
Dominika Raś, Csaba Csuzdi, Anna Z. Urbisz

et al.

The European Zoological Journal, Journal Year: 2025, Volume and Issue: 92(1), P. 97 - 122

Published: Jan. 13, 2025

Earthworm (Megadrili) ovaries are paired organs located usually in the XIII segment. So far, only their localization and general morphology well known. Light, fluorescence, electron microscopy analyses were carried out to describe ovary histology ultrastructure selected representatives of family Lumbricidae. We studied 12 species from six genera: Aporrectodea, Dendrobaena, Eisenia, Lumbricus, Octolasion, Proctodrilus. It was revealed that Lumbricidae tiny, leaf-like structures with one string consisting growing oocytes. Morphologically, can be divided into a proximal part (much wider attached septum) distal (more narrow containing oocytes form an egg at end ovary). Ovaries built germ cells united numerous syncytial cysts accompanied by somatic cells. Clustering interconnected via intercellular bridges poorly developed central cytoplasmic mass (reticular cytophore). According histological ultrastructural results, three zones. Zone I contains oogonia early-meiotic already cysts, which all develop synchronously. In zone II, enter diplotene lose synchrony; most probably, cell detaches given cyst, continues meiosis, gathers nutrients, grows, becomes oocyte, whereas rest do not grow become nurse III consists (vitellogenic) interconnecting enveloped follicular Such organization is known as "Dendrobaena" type. Although there minor differences between species, no found internal organization. Thus, it postulated characteristic lumbricids. Among earthworms, occur hormogastrids probably other families closely related

Language: Английский

Citations

0
A hormone-to-neuropeptide pathway inhibits sexual receptivity in immature Drosophila females DOI Creative Commons
Jie Chen, Pei‐Wen Zhu,

Sihui Jin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(8)

Published: Feb. 21, 2025

Newborns, typically asexual, undergo a process of sexual transition to reach maturity, but the regulatory mechanism underlying this is not clear. Here, we studied how female behavior modulated during by hormones and neuromodulators in Drosophila . We found that neuropeptide Leucokinin (LK) inhibits receptivity specifically period immature females, younger or mature females. Moreover, steroid hormone ecdysone, which mainly synthesized ovary maturation, acts on LK neurons via ecdysone receptor suppress receptivity. further suppresses through its LKR central pC1 neurons, decision center for behavior. These findings reveal hormone-to-neuropeptide pathway maturation , shedding light coordinate development behaviors.

Language: Английский

Citations

0
The Vast Utility of Drosophila Oogenesis DOI
Michelle S. Giedt, Tina L. Tootle

Methods in molecular biology, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 36

Published: Jan. 1, 2023

Language: Английский

Citations

9