Thiamine-modified metabolic reprogramming of human pluripotent stem cell-derived cardiomyocyte under space microgravity

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: April 7, 2024

During spaceflight, the cardiovascular system undergoes remarkable adaptation to microgravity and faces risk of cardiac remodeling. Therefore, effects mechanisms on morphology, physiology, metabolism, cellular biology need be further investigated. Since China started constructing Space Station (CSS) in 2021, we have taken advantage Shenzhou-13 capsule send human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) Tianhe core module CSS. In this study, hPSC-CMs subjected space showed decreased beating rate abnormal intracellular calcium cycling. Metabolomic transcriptomic analyses revealed a battery metabolic remodeling especially thiamine metabolism. The condition blocked intake hPSC-CMs. decline utilization under or by its antagonistic analog amprolium affected process tricarboxylic acid cycle. It ATP production, which led cytoskeletal homeostasis imbalance More importantly, vitro vivo studies suggest that supplementation could reverse adaptive changes induced simulated microgravity. This study represents first astrobiological lays solid foundation for aerospace biomedical research. These data indicate intervention thiamine-modified reprogramming during spaceflight might feasible countermeasure against

Language: Английский

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The Tricarboxylic Acid Cycle as a Central Regulator of the Rate of Aging: Implications for Metabolic Interventions

Advanced Biology, Journal Year: 2023, Volume and Issue: 7(7)

Published: May 2, 2023

Abstract Certain metabolic interventions such as caloric restriction, fasting, exercise, and a ketogenic diet extend lifespan and/or health span. However, their benefits are limited connections to the underlying mechanisms of aging not fully clear. Here, these explored in terms tricarboxylic acid (TCA) cycle (Krebs cycle, citric cycle) suggest reasons for loss effectiveness ways overcoming it. Specifically, deplete acetate likely reduce conversion oxaloacetate aspartate, thereby inhibiting mammalian target rapamycin (mTOR) upregulating autophagy. Synthesis glutathione may provide high‐capacity sink amine groups, facilitating autophagy, prevent buildup alpha‐ketoglutarate, supporting stem cell maintenance. Metabolic also accumulation succinate, slowing DNA hypermethylation, repair double‐strand breaks, reducing inflammatory hypoxic signaling, lowering reliance on glycolysis. In part through mechanisms, decelerate aging, extending lifespan. Conversely, with overnutrition or oxidative stress, processes function reverse, accelerating impairing longevity. Progressive damage aconitase, inhibition succinate dehydrogenase, downregulation hypoxia‐inducible factor‐1 α , phosphoenolpyruvate carboxykinase (PEPCK) emerge potentially modifiable interventions.

Language: Английский

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The Role of Dietary Antioxidants, Food Supplements and Functional Foods for Energy Enhancement in Healthcare Professionals

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1508 - 1508

Published: Dec. 10, 2024

Healthcare professionals frequently experience significant work overload, which often leads to substantial physical and psychological stress. This stress is closely linked increased oxidative a corresponding decline in energy levels. scoping review investigates the potential impact of dietary antioxidants food supplements conjunction with diet controlling these negative effects. Through an analysis biochemical pathways involved metabolism, paper emphasizes effectiveness targeted interventions. Key antioxidants, such as vitamins C E, polyphenols, carotenoids, are evaluated for their ability counteract enhance Additionally, assesses various supplements, including omega-3 fatty acids, coenzyme Q10, ginseng, mechanisms action enhancement. Practical guidelines incorporating energy-boost strategies into routine healthcare provided, emphasizing importance modifications reducing improving overall well-being performance high-stress environments. The concludes by suggesting directions future research validate findings explore new interventions that may further support under overload.

Language: Английский

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Association between diabetes and thiamine status - A systematic review and meta-analysis

Metabolism, Journal Year: 2023, Volume and Issue: 144, P. 155565 - 155565

Published: April 22, 2023

Language: Английский

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Citrate capped silver nanoparticles as an instantaneous colorimetric selective sensor for neomycin and thiamine in wastewater

New Journal of Chemistry, Journal Year: 2022, Volume and Issue: 46(29), P. 14081 - 14090

Published: Jan. 1, 2022

A simple colorimetric selective detection of antibacterial and vitamin drugs has been performed using citrate capped silver nanoparticles.

Language: Английский

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Thiamine deficiency in US veterans with obesity

Obesity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Introduction Thiamine deficiency is common after bariatric surgery, but patients with obesity may be deficient in thiamine even before surgery. The purpose of this research was to determine the prevalence at a medical weight‐management clinic and assess relationship between recent weight loss deficiency. Methods For observational study, records were reviewed for ( n = 146) nonsurgical medicine preoperative surgery Veterans Affairs Medical Center January 1, 2012, 31, 2019. defined as value less than test reference range. χ 2 tests used differences by race, gender, type diabetes status. Logistic regression evaluate Results found 32.2% patients. There no or Weight associated increased risk deficiency, although not statistically significant (odds ratio 2.04, 95% CI: 0.79–5.27). Conclusions Approximately one‐third evaluated had result indicating All people benefit from additional nutritional screening.

Language: Английский

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Post-traumatic stress disorder, metabolic syndrome, diabetic distress, and vitamin B1/benfotiamine

INTERNATIONAL NEUROLOGICAL JOURNAL, Journal Year: 2025, Volume and Issue: 21(1), P. 96 - 107

Published: March 20, 2025

A person with post-traumatic stress disorder (PTSD) is more likely to develop metabolic syndrome (MetS), depression, and diabetic distress. It also raises the risk of cardiometabolic neurodegenerative diseases (NDD). At same time, type 2 diabetes mellitus MetS can cause development core neurosis-like psychiatric symptoms characteristic PTSD. Chronic associated cardiovascular diseases, diabetes, NDD. More people think that mitochondrial dysfunction, peripheral/central chronic low-grade inflammation (CLGI), oxidative are main causes these diseases. Pathophysiological processes link them together. To make things better for patients, interventions aim control CLGI may be better, following ideas precision medicine. Deficiency certain biofactors, in particular, vitamin B1, an increased MetS, Targeted effects on CLGI, stress, metabolism disorders suggest use antioxidants, particularly B1/benfotiamine (BFT), have a positive effect not only course comorbid but manifestations Exogenous or BFT, corrects thiamine status disorders. BFT exhibits potent animal models NDD, stress-induced anxiety, aggression, depression. Thus, considered potentially safe cost-effective drug treatment many central nervous system Despite its aspects, therapeutic potential remains limited, as beneficial require high doses over long period time. Dibenzoylthiamine, lipophilic form has been shown anti-inflammatory antioxidant at much lower than both vitro vivo. However, preclinical clinical studies needed sure viability dibenzoylthiamine. This review pays extra attention analysis features biological role mechanism action, especially impact glucose function, state neuroinflammation, how dibenzoylthiamine protect neurons. We conducted search Scopus, Science Direct (from Elsevier), PubMed, MEDLINE databases. The keywords used were “thiamine”, “benfotiamine”, “dibenzoylthiamine”, “post-traumatic disorder”, “metabolic syndrome”, “diabetic distress”, “diabetes mellitus”. manual bibliography publications pinpoint research results eluded online search.

Language: Английский

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Unraveling the Molecular Mechanisms of Vitamin Deficiency in Alzheimer's Disease Pathophysiology

Aging and Health Research, Journal Year: 2025, Volume and Issue: unknown, P. 100226 - 100226

Published: March 1, 2025

Language: Английский

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The Pivotal Role of Thiamine Supplementation in Counteracting Cardiometabolic Dysfunctions Associated with Thiamine Deficiency

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3090 - 3090

Published: March 27, 2025

The isolation, structural elucidation, and synthesis of pure thiamin were achieved in 1936, marking a milestone vitamin research. As an organic compound soluble water, is essential for carbohydrate metabolism plants animals, its active form-as part the pyrophosphate coenzyme-performs these functions. Thereby, represents to human health involved several pathways that regulate pathophysiological mechanisms. Cardiovascular disease significantly impacted by thiamine imbalance supplementation offers substantial improvements associated conditions. In this comprehensive review, we aimed examine dual role deficiency accumulation, focusing on analysis causes deficiency. We detailed effects cardiovascular risk heart failure, explaining molecular mechanisms metabolic dysfunction, highlighting B1 diabetes mellitus management atherosclerosis development progression. Indeed, counteracts oxidative stress inflammation, ameliorating glycemic lipemic profiles. Additionally, reported beneficial counteracting cardiotoxicity induced cancer therapy. Although preclinical data strongly support benefits thiamine, clinical trial findings are contrast contradictory, hampered limitations such as small sample sizes inadequate follow-up. Further research needed investigate thiamine's potential benefits, overcoming current study evaluating use supplemental therapy alongside standard treatments different high-cardiovascular-risk

Language: Английский

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Instrument Multiplexing: Amplified Throughput for Measurement of Vitamin B1 in Whole Blood Using Parallel Liquid Chromatography Systems Interfaced to a Single Mass Spectrometer

Clinical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

Abstract Background Thiamine diphosphate (TDP), the active form of vitamin B1, plays an essential role in energy metabolism. TDP is analyzed clinical laboratory to assess nutritional status individuals at risk deficiency. In recent years, demand for B1 testing has increased dramatically, prompting implementation a high-throughput assay. We developed method using rapid sample preparation and multiplex electrospray LC-MS/MS analysis. Methods Whole blood samples were deproteinized trichloroacetic acid after addition isotope-labeled analyte (TDP-d3). was separated by reversed-phase chromatography on extended pH, trifunctional silane-bonded C18 columns positive ionization multiple reaction monitoring mass spectrometry. The system consisted 4 LC instruments plumbed single spectrometer. eluted 3.15 ± 0.08 min with run time 9.0 stream; results streams produced every 2.25 min. Passivation required optimize sensitivity peak quality. Results linear from 20 1000 nmol/L. Spike-recovery experiments showed accuracy ±15%. intra- inter-day assay imprecision ≤3%. Repeated injections calibrators QC materials across four excellent parity (<2% imprecision). No carryover detected. Each plate 81 4.5 h. Conclusions An accurate, specific, validated measure whole blood. Simple, fast employed adaptation staggered injection, LC-stream platform, which minimized spectrometer idle significantly improved efficiency.

Language: Английский

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