Targeting mitochondria in cancer therapy: Insight into photodynamic and photothermal therapies

Life Sciences, Journal Year: 2022, Volume and Issue: 307, P. 120898 - 120898

Published: Aug. 18, 2022

Language: Английский

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Unveiling the dark side of glucose-regulated protein 78 (GRP78) in cancers and other human pathology: a systematic review

Molecular Medicine, Journal Year: 2023, Volume and Issue: 29(1)

Published: Aug. 21, 2023

Abstract Glucose-Regulated Protein 78 (GRP78) is a chaperone protein that predominantly expressed in the lumen of endoplasmic reticulum. GRP78 plays crucial role folding by assisting assembly misfolded proteins. Under cellular stress conditions, can translocate to cell surface (csGRP78) were it interacts with different ligands initiate various intracellular pathways. The expression csGRP78 has been associated tumor initiation and progression multiple cancer types. This review provides comprehensive analysis existing evidence on roles types other human pathology. Additionally, discusses current understanding mechanisms underlying GRP78's involvement tumorigenesis advancement. Furthermore, we highlight recent innovative approaches employed downregulating cancers as potential therapeutic target.

Language: Английский

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HSPA1A inhibits pyroptosis and neuroinflammation after spinal cord injury via DUSP1 inhibition of the MAPK signaling pathway

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Feb. 9, 2025

Abstract Background Inflammation and proinflammatory programmed cell death, referred to as pyroptosis, are important causes of poor functional recovery after traumatic spinal cord injury (TSCI). Heat shock protein family A member 1A (HSPA1A) is a molecular chaperone that highly expressed TSCI thought be neuroprotective. However, the mechanisms underlying protective effects HSPA1A unclear. Methods The levels pyroptosis inflammation were determined by enzyme-linked immunosorbent assay (ELISA) western blotting analysis. role in regulating was verified vivo vitro experiments. mechanism elucidated bioinformatics coimmunoprecipitation analyses. Results Pyroptosis significantly increased TSCI. overexpression microglia attenuated nigericin- lipopolysaccharide (LPS)-induced vitro, whereas knockdown aggravated inflammation. In vivo, reduced tissue damage, nerve rats promoted recovery. Mechanistically, we identified interacts with dual specificity phosphatase 1 (DUSP1) inhibits activation mitogen-activated kinase (MAPK) pathway, thereby attenuating Conclusion reduces upregulating DUSP1 inhibiting MAPK pathway activation. has potential therapeutic approach promote

Language: Английский

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The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7792 - 7792

Published: July 14, 2022

Breast cancer (BC) is a major public health problem, with key pieces of information needed for developing preventive and curative measures still missing. For example, the participation chaperone system (CS) in carcinogenesis anti-cancer responses poorly understood, although it can be predicted to crucial factor these mechanisms. The chief components CS are molecular chaperones, here we discuss four them, Hsp27, Hsp60, Hsp70, Hsp90, focusing on their pro-carcinogenic roles BC potential anti-BC therapies. These chaperones targets negative chaperonotherapy, namely elimination/blocking/inhibition chaperone(s) functioning favor BC, using, instance, Hsp inhibitors. also employed immunotherapy against as adjuvants, together antigens. Extracellular vesicles (EVs) diagnosis management briefly discussed, considering easily accessible carriers biomarkers shippers agents amenable manipulation controlled delivery. data surveyed from many laboratories reveal that, enhance understanding role BS pathogenesis, one must consider physiological system, encompassing diverse members throughout body interacting ubiquitin–proteasome chaperone-mediated autophagy machinery, immune (IS). An integrated view CS, including its functional partners highly dynamic nature EVs transporting reach all cell compartments which they needed, opens yet unexplored pathways leading that interference by treatments centered components, such chaperones.

Language: Английский

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Factors and molecular mechanisms of radiation resistance in cancer cells

International Journal of Radiation Biology, Journal Year: 2022, Volume and Issue: 98(8), P. 1301 - 1315

Published: Feb. 28, 2022

The aim of this work is to review the published studies on radiation resistance mechanisms and molecular markers involved in different tumors. revision has been focused last 5 years (2016-2021).Radioresistance a cause concern as it causes failure therapy subsequent tumor relapse. Combination chemotherapy are clinically successful treating many types Despite continued improvements cancer treatment, locoregional recurrence or metastatic spread continues occur high proportion patients after being treated with combination treatments. There strong evidence that stem cells contribute resistance, contributing treatment failure. tumors not fully understood. A better understanding associated signaling pathways regulate will open up new strategies for by therapy. Radiation can damage malignant mainly induction DNA double-strand breaks. However, some appear resistant repopulate following leading over time treatment. Native induced resistance. It described numerous acting through action such apoptosis alterations cell growth, proliferation repair, hypoxia, increase invasiveness migration capacity, cycle alterations, expression heat shock proteins, among others. Therefore, multifactorial phenomenon that, types, occurs regulatory which molecules intervene. Resistance be acquired altering knowledge could help classification more aggressive

Language: Английский

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Anticarcinogenic Effects of Gold Nanoparticles and Metformin Against MCF-7 and A549 Cells

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: 202(10), P. 4494 - 4507

Published: Feb. 15, 2024

Abstract Metformin is commonly prescribed to people with diabetes. has been shown in previous studies be able prevent the growth of cancer cells. This study aims investigate effects metformin and gold nanoparticles MCF7 breast A549 lung cell lines. The on cells were determined grown 24 h culture. MCF-7 incubated for treatment escalating molar concentrations ifosfamide. MTT assay was used determine cytotoxicity toward expression Bax, BCL2, PI3K, Akt3, mTOR, Hsp60, Hsp70, TNF-α measured by RT-PCR. inhibited proliferation a dose time-dependent manner an IC50 value 5 µM 10 µg/mL. RT-PCR assays showed ifosfamide + significantly reduced Hsp60 Hsp70 increased Bax. findings obtained this suggest that further should conducted, can treatments.

Language: Английский

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Effect of tannic acid on doxorubicin‐induced cellular stress: Expression levels of heat shock genes in rat spleen

Biotechnology and Applied Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: June 30, 2024

Doxorubicin (DOX), an anthracycline group antibiotic, has been extensively employed as a potent chemotherapeutic agent for treating solid and hematopoietic tumors in humans. Amid exposure to diverse stress conditions, living organisms swiftly initiate the synthesis of heat shock proteins (HSPs), set highly conserved proteins. Tannic acid (TA) garnered increasing study attention due its special chemical properties, health benefits, wide availability. This study's primary aim is elucidate impact DOX TA on expression levels Hsp90aa1, Hspa1a, Hspa4, Hspa5 spleen tissues rats. Sprague Dawley rats (Rattus norvegicus, male, 9-10 weeks old, 180 ± 20 g) were randomly divided into 4 groups: control, (30 mg/kg cumulative), (50 mg/kg), + (5 50 mg/kg, respectively). Subsequently, collected from rats, complementary DNA libraries generated after application process. The quantitative real-time PCR method was used detect quantify mRNA changes genes our results showed that expressions targeted up-regulated rat exposed DOX. However, this increase remarkably suppressed by treatment. These findings suggest may serve protective agent, mitigating toxic effects spleen.

Language: Английский

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Nanomaterial-mediated low-temperature photothermal therapy via heat shock protein inhibition

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 11, 2022

With the continuous development of nanobiotechnology in recent years, combining photothermal materials with nanotechnology for tumor therapy (PTT) has drawn many attentions nanomedicine research. Although nanomaterial-mediated PTT is more specific and targeted than traditional treatment modalities, hyperthermia can also damage normal cells. Therefore, researchers have proposed concept low-temperature PTT, which expression heat shock proteins (HSPs) inhibited. In this article, research strategies years based on inhibition HSPs to achieve was reviewed. Folowing this, synthesis, properties, applications these nanomaterials were introduced. addition, we summarized problems at stage provided an outlook future directions.

Language: Английский

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Targeting HSP70 chaperones by rhein sensitizes liver cancer to artemisinin derivatives

Phytomedicine, Journal Year: 2023, Volume and Issue: 122, P. 155156 - 155156

Published: Oct. 20, 2023

Language: Английский

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The Confluence of Nanotechnology and Heat Shock Protein 70 in Pioneering Glioblastoma Multiforme Therapy: Forging Pathways Towards Precision Targeting and Transformation

Advances in Pharmacological and Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Heat-shock protein 70 (HSP70) and nanotechnology have emerged as promising avenues in glioblastoma multiforme (GBM) therapy, addressing the critical challenges posed by its aggressive nature therapeutic resistance. HSP70's dual role cellular stress response tumour survival emphasises potential both a biomarker target. This review explores innovative integration of HSP70 with nanotechnology, emphasising advancements imaging, drug delivery combination therapies. Nanoparticles, including SPIONs, liposomes, gold nanoparticles metal-organic frameworks, demonstrate enhanced targeting efficacy through modulation. Functionalized nanocarriers exploit tumour-specific overexpression to improve delivery, minimise off-target effects overcome blood-brain barrier. Emerging strategies such chemophototherapy, immunotherapy photothermal therapy leverage interactions within microenvironment, enabling synergistic treatment modalities. The also highlights translational challenges, heterogeneity GBM, regulatory hurdles variability permeability retention (EPR) effect. Integrating computational modelling, personalised approaches adaptive trial designs is crucial for clinical translation. By bridging molecular biology, HSP70-targeted hold transformative redefine GBM diagnosis treatment, offering hope improved quality life. Trial Registration: ClinicalTrials.gov identifier: NCT00054041 NCT04628806.

Language: Английский

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