Capacitation of ram spermatozoa promotes changes in energy metabolism and aquaporin 3 and is affected by individual testosterone variations

Andrology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Background Recently, the metabolic pathways involved in energy production and role of aquaglyceroporins capacitation‐associated events have been studied humans mice. However, little is known about these ram spermatozoa. Objective The present study investigated bioenergetic aquaglyceroporin 3 variations during vitro capacitation In addition, differences testosterone levels between males were examined to determine their influence on capacitation‐like changes. Materials methods Spermatozoa obtained from nine rams (ejaculates = 36) incubated for 180 min three different media (control, capacitating, aquaglyceroporin‐inhibitor media) at 38.5°C. At 0 incubation each medium, sperm viability, kinetics, chlortetracycline patterns, adenosine triphosphate concentration, lactate excretion (final subproduct glycolysis), immunolocalization aquaporin evaluated. Results increment capacitated spermatozoa‐chlortetracycline pattern hyperactivated‐like movement characterized by highest curvilinear velocity amplitude lateral head displacement lowest linearity was only recorded after capacitating medium. this time conditions, content decreased, whereas location midpiece principal piece increased compared min. Such changes not observed control medium over time. Incubation reduced drastically motility other media. Testosterone analysis revealed a significant individual variability, which also all parameters Furthermore, negatively correlated with but positively levels, motility, sperm‐chlortetracycline pattern, immunolabeling piece. Conclusion Despite differences, spermatozoa increases consumption, metabolism, piece, seems be related acquisition motility. may serve as valuable tool select those greater metabolism rate fertilizing capacity.

Language: Английский

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Manipulation of metabolism to improve liquid preservation of mammalian spermatozoa

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 271, P. 107631 - 107631

Published: Nov. 2, 2024

Reproductive success in mammals hinges on the ability of sperm to generate sufficient energy through cellular metabolism perform energy-intensive processes required for fertilisation, including motility, maturation, and oocyte interactions. It is now widely accepted that exhibit metabolic flexibility, utilising a combination glycolysis oxidative phosphorylation (supported by Krebs cycle other complementary pathways) meet their demands. However, preferred pathway production varies significantly among species, making it challenging map species-specific strategies, particularly species with high like ram. Additionally, differences methodologies used measure have led biased interpretations species' complicating development liquid storage methods aimed at preserving spermatozoa manipulating generation based requirements. This review examines requirements, current assessing capacity, research metabolism. Future should focus establishing standardised approach determining preferences accurately critical step before developing effective preservation methods. By identifying regulatory points, strategies can be designed temporarily inhibit pathways, conserving resources reducing accumulation by-products. Alternatively, supplementation depleted metabolites guided understanding areas excessive consumption during prolonged Applying this knowledge develop tailored techniques will help minimise damage improve survival vitro processing storage, ultimately enhancing artificial breeding programs.

Language: Английский

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Bioenergetic changes in response to sperm capacitation and two-way metabolic compensation in a new murine model

Cellular and Molecular Life Sciences, Journal Year: 2022, Volume and Issue: 80(1)

Published: Dec. 19, 2022

The acquisition of fertilizing ability by mammalian spermatozoa, known as "capacitation," includes processes that depend on particular metabolic pathways. This has led to the hypothesis ATP demands might differ between capacitated and non-capacitated cells. Mouse sperm can produce via OXPHOS aerobic glycolysis, an advantageous characteristic considering these cells have function in complex variable environment female reproductive tract. Nonetheless, despite evidence showing both pathways play a role events associated with mouse capacitation, there is contradictory regarding changes promoted capacitation this species. In addition, vast majority studies murine metabolism use Mus musculus laboratory strains model, thus neglecting wide diversity traits other species Mus. Focus closely related distinct evolutionary histories, which may be result different selective pressures, could shed light processes. Here, we analyzed variations bioenergetics spermatozoa steppe mouse, spicilegus, high performance. Furthermore, compared similar previously characterized M. musculus. We found spicilegus responded manner sperm. However, showed features, including perform cross-pathway compensation response either respiratory or glycolytic inhibition, revealing delicate fine-tuning its capacities.

Language: Английский

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Effect of Motility Factors D-Penicillamine, Hypotaurine and Epinephrine on the Performance of Spermatozoa from Five Hamster Species

Biology, Journal Year: 2022, Volume and Issue: 11(4), P. 526 - 526

Published: March 30, 2022

Assessments of sperm performance are valuable tools for the analysis fertilizing potential and to understand determinants male fertility. Hamster species constitute important animal models because they produce cells in high quantities quality. Sexual selection over evolutionary time these seems have resulted largest mammalian spermatozoa, swimming bioenergetic performances. Earlier studies showed that golden hamster requires motility factors such as D-penicillamine, hypotaurine epinephrine (PHE) sustain survival time, but it is unknown how affect kinetics or ATP levels if other also require them. The objective present study was examine effect PHE on spermatozoa five (Mesocricetus auratus, Cricetulus griseus, Phodopus campbelli, P. sungorus, roborovskii). In incubated up 4 h without with PHE, we assessed motility, viability, acrosome integrity, velocity trajectory, content. results differences among species. They had a significant positive maintenance quality M. auratus C. whereas there no consistent Differences between may be result varying underlying regulatory mechanisms relate successful fertilization.

Language: Английский

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Genetic Ablation of Na,K-ATPase α4 Results in Sperm Energetic Defects

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: May 26, 2022

The Na,K-ATPase alpha 4 isoform (NKAα4) is expressed specifically in the male germ cells of testes and particularly abundant mature spermatozoa. Genetic deletion NKAα4 mice (NKAα4 KO mice) results complete infertility male, but not female mice. reduced fecundity due to a series defects, including severe impairment total hyperactive sperm motility. In this work, we show that also leads major defects metabolism energetics. Thus, compared wild-type sperm, from display significant reduction extracellular acidification rate (ECAR), indicative impaired glycolytic flux. addition, mitochondrial function disrupted lacking NKAα4, as indicated by membrane potential lower oxygen consumption (OCR). Moreover, ratio between oxidized forms nicotinamide adenine dinucleotide (NAD/NADH) increased indicating shift cellular redox state. These metabolic changes are associated with augmented reactive species (ROS) production lipid peroxidation sperm. Altogether, these findings reveal novel link activity energetics, highlighting essential role ion transporter physiology.

Language: Английский

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Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(23), P. 15247 - 15247

Published: Dec. 3, 2022

In order to sustain motility and prepare for fertilization, sperm require energy. The characterization of ATP production usage in mouse species revealed substantial differences metabolic pathways that can be differentially affected by capacitation. Moreover, spermatozoa encounter different environments with varying viscoelastic properties the female reproductive tract. Here, we examine whether viscosity affects levels kinematics during capacitation vitro. Sperm from three (Mus musculus, M. spretus, spicilegus) were incubated under capacitating conditions a modified Tyrode's medium containing bicarbonate, glucose, pyruvate, lactate, bovine serum albumin (mT-BH) or bicarbonate-free as non-capacitating control. Viscosity was increased inclusion polyvinylpyrrolidone. measured bioluminescence kit, examined computer-aided analysis system. musculus sperm, declined capacitation, but no found between sperm. contrast, spretus spicilegus, decreased Increasing did not modify timing proportion cells undergoing result additional time- concentration-dependent decreases spicilegus conditions. Additionally, altered both velocity trajectory descriptors. limited impact higher on could related low intensity postcopulatory sexual selection this species. Responses seen other two linked ability their perform better enhanced selective pressures.

Language: Английский

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On the Origin and Evolution of Sperm Cells

Cells, Journal Year: 2022, Volume and Issue: 12(1), P. 159 - 159

Published: Dec. 30, 2022

Sperm cells have intrigued biologists since they were first observed nearly 350 years ago by Antonie van Leeuwenhoek and Johan Ham [...]

Language: Английский

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The effect of cryopreservation media on the quality of β-thalassemia mouse spermatozoa

Open Veterinary Journal, Journal Year: 2022, Volume and Issue: 12(5), P. 602 - 602

Published: Jan. 1, 2022

Background: The mouse model of human diseases is commonly used for biomedical study, including β-thalassemia (β-thal), an inherited hemoglobin disorder. Maintaining the mice strain by natural mating systems costly and seems impractical, especially during COVID-19 pandemic. Sperm freezing a cost-effective solution β-thal colony management. Aim: present study was conducted to determine appropriate cryopreservation media spermatozoa establish sperm bank. Methods: epididymal C57BL/6 wild-type (WT) -globin gene knockout thalassemia (BKO) were frozen in four media: I) raffinose-skim milk-monothioglycerol (MTG), II) milk-glutamine, III) raffinose-egg yolk-glycerol, IV) egg yolk-TES-Tris. quality assessed prior following freeze-thawing. Results: Compared with WT counterparts, viable before exhibiting elevated levels oxidative stress significantly greater BKO (p = 0.01). After thawing, membrane integrity preserved I lower 0.001). viability males also inferior when III IV 0.008 p 0.027). amount spermatozoon I, III, 0.002 0.044). Comparing media, motility acrosome higher than those other < 0.001 Spermatozoa highest mitochondrial potential (MMP) observed both genotypes 0.012 > 0.05). viability, integrity, post-thaw did not differ among solutions. Conclusion: Irrespective are rather more sensitive WT. Raffinose-skim milk-MTG/glutamine, yolk-TES-Tris can all be preserve spermatozoa. However, slightly better characteristics, may diluent choice cryopreservation. addition reducing agent thawing strongly recommended efficiently prevent therefore improve frozen-thawed survival.

Language: Английский

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Unveiling the Secrets of Oocyte Fertilisation Failure, Is Calcium the Only Solution?!: A mini-review

Journal of Reproductive Medicine and Embryology, Journal Year: 2024, Volume and Issue: 0(0), P. 0 - 0

Published: Jan. 22, 2024

Total fertilization failure (TFF) refers to the complete inability of all oocytes in a human IVF cycle undergo fertilization, even after administration intracytoplasmic sperm injection therapy (ICSI). Currently, oocyte activation insufficiency could explain occurrence TFF; however, its mechanism remains unclear. Calcium signaling plays crucial role events. Following from humans and animals exhibited notable calcium oscillations. Abnormal oscillations following be primary cause TFF. Examining literature revealed numerous attempts investigate discover an effective solution for that alarming occurrence. The most commonly used regimen overcome deficiency (OAD) is addition Calcium, which cannot induce proper oscillation needed fertilization. This review seeks address issue (FF) by examining various factors associated with this investigating available techniques artificial ICSI.

Language: Английский

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Evaluación de los efectos de la endogamia en la calidad seminal bovina mediante el análisis de bloques de homocigosidad y estudios de asociación de genoma completo

Published: March 6, 2024

La endogamia puede definirse como el apareamiento entre individuos emparentados. Su aumento provocar depresión endogámica, que es la disminución de media fenotípica poblacional varios caracteres, principalmente los reproductivos. En últimos años, utilización información genómica ha permitido estimar forma más precisa nivel endogamia, mediante identificación segmentos homocigotos (runs of homozygosity, ROH) y, en base a ellos poder calcular un coeficiente molecular (FROH). esta tesis se caracterizó abundancia y distribución ROH tres poblaciones bovinas, encontrándose variabilidad, acumulación ciertas regiones dos razas, las cuales asociaron con procesos selección. Particularmente, observó variabilidad similares coeficientes basados pedigrí. Además, evaluó efecto global, cromosómica por longitud, sobre morfometría, motilidad longevidad espermática, así genómicas enriquecidas asociadas dichos caracteres. Se encontró una mayor 0,125 provocaba incremento espermatozoides pequeños alargados, hiperactivación temprana, cual podría limitar espermática. Las significativamente incluyeron genes asociados espermatogénesis, motilidad, fisiología espermática desarrollo embrionario. evidenciaron diferentes niveles endogámica distintos cromosomas, debido largos, originados recientemente. Finalmente, combinación análisis asociación permitió identificar potencialmente involucrados conclusión, uso FROH fiable e informativo F basado pedigrí, complementado funcional metodología innovadora para caracteres productivos. Esta contribuye aumentar conocimiento efectos calidad del bovino.

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