Deciphering the Genetic Mechanisms of Salt Tolerance in Sorghum bicolor L.: Key Genes and SNP Associations from Comparative Transcriptomic Analyses DOI Creative Commons
Donghyun Jeon, Jin‐Baek Kim,

Beum‐Chang Kang

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(14), P. 2639 - 2639

Published: July 13, 2023

Sorghum bicolor L. is a vital cereal crop for global food security. Its adaptability to diverse climates make it economically, socially, and environmentally valuable. However, soil salinization caused by climate extremes poses threat sorghum. This study aimed identify candidate salt-tolerant genes single nucleotide polymorphisms (SNPs) performing comparative transcriptome analysis on mutant sorghum line its wild type. The was generated through gamma ray exposure selection salt tolerance. Phenotypic measurements were taken, followed mRNA sequencing variant calling. In this study, potential non-synonymous SNPs associated with tolerance inferred, including LOC8071970, LOC8067721, LOC110430887, LOC8070256, LOC8056880. These demonstrated notable differences in nsSNPs comparison the type, suggesting their roles Additionally, LOC8060874 (cyanohydrin beta-glucosyltransferase) suggested as key gene involved due possible role dhurrin biosynthesis under stress. upcoming research, additional reverse genetics studies will be necessary order verify function of those relation conclusion, underscores significance investigating mechanisms Our findings may provide insights future breeding strategies at enhancing salinity productivity.

Language: Английский

SWEET11b transports both sugar and cytokinin in developing barley grains DOI Creative Commons
Volodymyr Radchuk, Zeinu Mussa Belew, André Gündel

et al.

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(6), P. 2186 - 2207

Published: March 1, 2023

Abstract Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b African clawed frog (Xenopus laevis) oocytes facilitated bidirectional transfer not only just sucrose and glucose, but also cytokinin. Barley plants harboring loss-of-function mutation could set viable grains, while distribution cytokinin was altered developing grains which gene knocked down. Sucrose allocation within transgenic disrupted, consistent with changes to gradient across as visualized by magnetic resonance imaging Fourier transform infrared spectroscopy microimaging. Decreasing expression reduced overall grain size, sink strength, number endopolyploid endosperm cells, contents starch protein. The control exerted over sugars cytokinins likely predetermines synergy, resulting adjustments grain's biochemistry transcriptome.

Language: Английский

Citations

37
Structure, evolution, and roles of SWEET proteins in growth and stress responses in plants DOI
Yongxing Zhu, Ye Tian, Shuo Han

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130441 - 130441

Published: Feb. 28, 2024

Language: Английский

Citations

11
Plasma membrane-localized hexose transporter OsSWEET1b, affects sugar metabolism and leaf senescence DOI
Qiang Zhang, Changzhao Chen, Rui Guo

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(1)

Published: Jan. 1, 2024

Language: Английский

Citations

10
Pivotal role of heterotrimeric G protein in the crosstalk between sugar signaling and abiotic stress response in plants DOI
Wenjiao Zou, Qian Yu,

Yu Ma

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 210, P. 108567 - 108567

Published: March 26, 2024

Language: Английский

Citations

10
Multi-Omics Approaches Against Abiotic and Biotic Stress—A Review DOI Creative Commons

Venkatramanan Varadharajan,

R. Radhika,

Pandiyan Muthuramalingam

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 865 - 865

Published: March 10, 2025

Plants face an array of environmental stresses, including both abiotic and biotic stresses. These stresses significantly impact plant lifespan reduce agricultural crop productivity. Abiotic such as ultraviolet (UV) radiation, high low temperatures, salinity, drought, floods, heavy metal toxicity, etc., contribute to widespread losses globally. On the other hand, those caused by insects, fungi, weeds, further exacerbate these challenges. stressors can hinder systems at various levels, molecular, cellular, development processes. To overcome challenges, multi-omics computational approaches offer a significant tool for characterizing plant’s biomolecular pool, which is crucial maintaining homeostasis signaling response changes. Integrating multiple layers omics data, proteomics, metabolomics, ionomics, interactomics, phenomics, simplifies study resistance mechanisms. This comprehensive approach enables regulatory networks pathway maps, identifying potential targets improving through genetic engineering or breeding strategies. review highlights valuable insights from integrating unravel stress responses factors. By decoding gene regulation transcriptional networks, techniques reveal critical mechanisms underlying tolerance. Furthermore, role secondary metabolites in bio-based products enhancing mitigation discussed. Genome editing tools promising strategies resilience, evidenced successful case studies combating stressors. whole, this extensively discusses advanced that aids understanding molecular basis developing novel improve crops’ organisms’ resilience

Language: Английский

Citations

1
Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants DOI Creative Commons
Ding Yuan, Xiaolei Wu,

Xiangqun Jiang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 221 - 221

Published: Feb. 9, 2024

Membrane transporters are proteins that mediate the entry and exit of substances through plasma membrane organellar membranes capable recognizing binding to specific substances, thereby facilitating substance transport. divided into different types, e.g., ion transporters, sugar amino acid aquaporins, based on they These inhibit reactive oxygen species (ROS) generation regulation, transport, hormone induction, other mechanisms. They can also promote enzymatic nonenzymatic reactions in plants, activate antioxidant enzyme activity, ROS scavenging. Moreover, transport plant growth regulators, solute proteins, redox potential involved metabolism corresponding metabolic pathways, ultimately achieving homeostasis plants. In turn, ROS, as signaling molecules, affect activity under abiotic stress collaboration with ions involvement pathways. The research described this review provides a theoretical basis for improving resistance, promoting development, breeding high-quality varieties.

Language: Английский

Citations

8
Transcriptional responses to salinity‐induced changes in cell wall morphology of the euryhaline diatom Pleurosira laevis DOI Creative Commons
Shiho Kamakura, Gust Bilcke, Shinya Sato

et al.

Journal of Phycology, Journal Year: 2024, Volume and Issue: 60(2), P. 308 - 326

Published: March 6, 2024

Abstract Diatoms are unicellular algae with morphologically diverse silica cell walls, which called frustules. The mechanism of frustule morphogenesis has attracted attention in biology and nanomaterials engineering. However, the genetic regulation morphology remains unclear. We therefore used transcriptome sequencing to search for genes involved centric diatom Pleurosira laevis , exhibits morphological plasticity between flat domed valve faces salinity 2 7, respectively. observed differential expression transposable elements (TEs) transporters, likely due osmotic response. Up‐regulation mechanosensitive ion channels down‐regulation Ca 2+ ‐ATPases cells valves suggested that cytosolic levels were changed morphologies. Calcium signaling could be a detecting pressure changes triggering shifts. also an up‐regulation ARPC1 annexin, actin filament dynamics known affect morphology, as well encoding frustule‐related proteins such BacSETs frustulin. Taken together, we propose model salinity‐induced morphogenetic driven by upstream responses, levels, downstream ‐dependent proteins. This study highlights sensitivity euryhaline diatoms environmental role active cellular processes controlling gross under different pressures.

Language: Английский

Citations

6
Advantageous spike-to-stem competition for assimilates contributes to the reduction in grain number loss in wheat spikes under water deficit stress DOI Creative Commons
Juan Li,

Zimeng Liang,

Yakun Li

et al.

Agricultural Water Management, Journal Year: 2024, Volume and Issue: 292, P. 108675 - 108675

Published: Jan. 18, 2024

This study was conducted to investigate the mechanisms of assimilate distribution and sugar metabolism in spike-stem that inhibit formation fertile florets grains per spike winter wheat under pre-reproductive drought stress. Two cultivars, CH58 (relatively strongly tolerant) LH6 weakly tolerant), were subjected successive soil treatments from jointing heading during 2020–2022 growing seasons. The results showed stress intensified degradation abortion florets. Compared CH58, decrease number increased by an average 5.3%−8.0% 8.3%−9.0%, respectively. Drought significantly inhibited 13C-photosynthates spikes (15.7%−24.7%) stems (8.5%−11.7%) booting stage. differentially expressed genes enriched starch sucrose metabolic pathways much higher stem than those CH58. reduced hexose young but fructan concentrations stems. LH6, invertase activity accompanying high expression transporter protein (STP) contributed utilization spikes. Additionally, severe drought, concentration transport proteins (SWEET SUT) improved ability assimilates thus alleviated loss grain spike. Exogenous spermidine optimized allocation after stress, thereby increasing Overall, dominant utilize compete for between contribute resistance stress-induced reduction chemicals can regulate through this pathway, promoting number.

Language: Английский

Citations

5
Cold Stress Response Mechanisms in Anther Development DOI Open Access

Borong Huang,

Yubo Fan,

Lijiao Cui

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 30 - 30

Published: Dec. 20, 2022

Unlike animals that can escape threats, plants must endure and adapt to biotic abiotic stresses in their surroundings. One such condition, cold stress, impairs the normal growth development of plants, which most phases reproductive are particularly susceptible external low temperature. Exposed uncomfortably temperature at stage, meiosis, tapetal programmed cell death (PCD), pollen viability, fertilization disrupted, resulting plant sterility. Of them, cold-induced dysfunction is main cause sterility by blocking nutrition supplements for microspore altering timely PCD. Further evidence has indicated homeostatic imbalances hormones, including abscisic acid (ABA) gibberellic (GA), sugars have occurred cold-treated anthers. Among stress gives rise accumulation ABA decrease active GA anthers affect represses transport sugar microspores. Therefore, evolved lots mechanisms alleviate damage mainly regulating phytohormone levels metabolism. Herein, we discuss physiological metabolic effects on male underlying from perspective molecular biology. A deep understanding response anther will provide noteworthy references cold-tolerant crop breeding production under stress.

Language: Английский

Citations

19
Physiological and Transcriptomic Evaluation of Drought Effect on Own-Rooted and Grafted Grapevine Rootstock (1103P and 101-14MGt) DOI Creative Commons
Davide Bianchi, Valentina Ricciardi, Carola Pozzoli

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(5), P. 1080 - 1080

Published: Feb. 28, 2023

Grapevines worldwide are grafted onto Vitis spp. rootstocks in order to improve their tolerance biotic and abiotic stresses. Thus, the response of vines drought is result interaction between scion variety rootstock genotype. In this work, responses genotypes were evaluated on 1103P 101-14MGt plants, own-rooted with Cabernet Sauvignon, three different water deficit conditions (80, 50, 20% soil content, SWC). Gas exchange parameters, stem potential, root leaf ABA transcriptomic investigated. Under well-watered conditions, gas potential mainly affected by grafting condition, whereas under sever they severe stress (20% SWC), showed an “avoidance” behavior. It reduced stomatal conductance, inhibited photosynthesis, increased content roots, closed stomata. The maintained a high photosynthetic rate, limiting reduction potential. This behavior results “tolerance” strategy. An analysis transcriptome that most differentially expressed genes detected at SWC, more significantly roots than leaves. A core set has been highlighted as being related not genotype nor grafting. Genes specifically regulated have identified well. 1103P, 101-14MGt, number both conditions. regulation revealed readily perceived scarcity rapidly faced stress, agreement its avoidance

Language: Английский

Citations

13