Genome-Wide Bioinformatics Analysis of SWEET Gene Family and Expression Verification of Candidate PaSWEET Genes in Potentilla anserina DOI Creative Commons
Javed Iqbal,

Wuhua Zhang,

Yingdong Fan

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(3), P. 406 - 406

Published: Jan. 30, 2024

Sugars act as the main energy sources in many fruit and vegetable crops. The biosynthesis transportation of sugars are crucial especially contribute to growth development. SWEET is an important gene family that plays a vital role plants’ growth, development, adaptation various types stresses (biotic abiotic). Although genes have been identified numerous plant species, there no information on SWEETs Potentilla anserina. In present study, we performed comprehensive genome-wide bioinformatics analysis total 23 candidate PaSWEETs anserina genome, which were randomly distributed ten different chromosomes. phylogenetic analysis, chromosomal location, structure, specific cis-elements, protein interaction network, physiological characteristics these systematically examined. results relationship with Arabidopsis thaliana revealed PaSWEET divided into four clades (I, II, III, IV). Moreover, tissue-specific expression through quantitative real-time polymerase chain reaction (qRT-PCR) validation exposed differentially expressed tissues (roots, stems, leaves, flowers). Mainly, relative fold swollen unswollen tubers effectively (7, 9, 12) highly (300-, 120-, 100-fold) tubers. To further elucidate function 12), their subcellular location was confirmed by inserting them tobacco it noted cell membrane. On basis overall results, suggested involved tuber formation P. crux, speculated our study provides valuable theoretical base for in-depth development enhancing molecular breeding

Language: Английский

Potato E3 ubiquitin ligase StRFP1 positively regulates late blight resistance by degrading sugar transporters StSWEET10c and StSWEET11 DOI
Xintong Wu,

Tianyu Lin,

Xiaoshuang Zhou

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(2), P. 688 - 704

Published: May 20, 2024

Summary Potato ( Solanum tuberosum ) is the fourth largest food crop in world. Late blight, caused by oomycete Phytophthora infestans , most devastating disease threatening potato production. Previous research has shown that StRFP1, a Arabidopsis Tóxicos en Levadura (ATL) family protein, positively regulates late blight resistance via its E3 ligase activity. However, underlying mechanism unknown. Here, we reveal StRFP1 associated with plasma membrane (PM) and undergoes constitutive endocytic trafficking. Its PM localization essential for inhibiting P. colonization. Through vivo vitro assays, investigated interacts two sugar transporters StSWEET10c StSWEET11 at PM. Overexpression (OE) of or enhances Both exhibit sucrose transport ability yeast, OE leads to an increased content apoplastic fluid leaves. ubiquitinates promote their degradation. We illustrate novel which ATL protein degrading susceptibility (S) factors, such as Sugars Will Eventually be Exported Transporters (SWEETs). This offers potential strategy improving utilizing host positive immune regulators neutralize S factors.

Language: Английский

Citations

4
Role of GmFRI-1 in Regulating Soybean Nodule Formation Under Cold Stress DOI Open Access
Hongcai Zhang, He Lin, Huiyun Li

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 879 - 879

Published: Jan. 21, 2025

Symbiotic nitrogen fixation, recognized as the most efficient assimilation system in ecosystems, is essential for soybean growth, nodulation provides critical to host cells. Soybeans thrive warm and moist environments. However, they are highly susceptible low temperatures, which impede formation development of root nodules. The genetic basis molecular mechanism underlying inhibition induced by temperatures remain unclear. In this study, we conducted a comparative transcriptomic analysis roots inoculated with rhizobium at 1 DPI (Day Post Inoculation) under normal or cold treatments. We identified 39 up-regulated 35 down-regulated genes associated fixation. Notably, cold-responsive including three FRI (Frigida) family were among differentially expressed (DEGs). Further expression pattern GmFRI-1 demonstrated it being significantly responsive inoculation its highest investigation revealed that overexpression led an increase nodule number, while RNA interference (RNAi)-mediated gene editing suppressed formation. Additionally, may regulate modulating GmNIN (NODULE INCEPTION), GmNSP1 (nodulation signaling pathway 1), GmHAP2-2 (histone- haem-associated protein domain) nod factor pathway. This study offers new insights into regulation stress legumes indicates serve key regulator stress.

Language: Английский

Citations

0
Transcription factor AtWRKY43 enhances drought tolerance by positively regulating the expression of AtSWEET5 DOI
Qijun Ma, Shuo Xu, Yu Sun

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109576 - 109576

Published: Jan. 1, 2025

Language: Английский

Citations

0
Transcriptome alterations related to heat stress responses of wild and cultivated barley DOI Creative Commons
Forouzan Bahrami, Ahmad Arzani,

Mehdi Rahimmalek

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 222, P. 109699 - 109699

Published: Feb. 26, 2025

Heat stress, exacerbated by global warming, threatens food security disrupting plant growth and productivity across many regions. The present study compared the transcriptome changes of heat-tolerant wild (Hordeum vulgare ssp. spontaneum L.) genotype heat-sensitive cultivated Hordeum ('Mona' cultivar) barley subjected to control (24 ± 2 °C) heat stress (40 °C, 3 h) conditions via RNA sequencing with Illumina Hiseq2500 platform. exhibited less impact from on physiology than 'Mona' cultivar. led 2141 differentially expressed genes (DEGs) in 1456 Gene ontology enrichment analysis DEGs revealed that biological processes such as defense response proline polyamine biosynthesis, oxidative scavenging were predominantly involved thermo-tolerance barley. Moreover, shock proteins, osmoprotectants, catalytic activity identified most critical molecular functions high temperatures significant alterations expression levels candidate highlight these genes' pivotal role Comparing evolved mechanisms between helps identify effective tolerance thermo-tolerant genotype.

Language: Английский

Citations

0
Novel genetic control of alpha-amylase activity during pre-harvest sprouting indicated by RNA sequencing of soft winter wheat varieties Cardinal and Logan DOI
Bryan W. Penning

Plant Gene, Journal Year: 2025, Volume and Issue: 42, P. 100495 - 100495

Published: Feb. 28, 2025

Language: Английский

Citations

0
Genome-wide exploration: evolution, structural characterization, molecular docking, molecular dynamics simulations and expression analysis of sugar transporter (ST) gene family in potato (Solanum tuberosum) DOI
Md. Sohel Mia,

Sourav Biswas Nayan,

Md. Numan Islam

et al.

Computational Biology and Chemistry, Journal Year: 2025, Volume and Issue: 117, P. 108402 - 108402

Published: March 1, 2025

Language: Английский

Citations

0
Beyond Energy: How Small‐Molecule Sugars Fuel Seed Life and Shape Next‐Generation Crop Technologies DOI

Bilquees Bozdar,

Nazir Ahmed,

Panfeng Tu

et al.

Journal of Agronomy and Crop Science, Journal Year: 2025, Volume and Issue: 211(2)

Published: March 1, 2025

ABSTRACT Small‐molecule sugars, such as glucose, sucrose, trehalose and raffinose, are essential for seed germination, seedling establishment stress resilience. These sugars act both energy sources signalling molecules, regulating osmotic balance, gene expression critical metabolic pathways involved in early growth. Key enzymes, including α‐amylase ( AMY1 AMY2 ), hexokinase HXK1 ) sucrose‐phosphate synthase (SPS), facilitate carbohydrate mobilisation during fueling glycolysis, the tricarboxylic acid (TCA) cycle pentose phosphate pathway (PPP). Hormonal interactions, particularly with gibberellins (GA) abscisic (ABA), integrate sugar metabolism developmental environmental cues. Sugar transport proteins, Sugars Will Eventually be Exported Transporters (SWEET), Sucrose (SUT) Tonoplast (TST), ensure efficient distribution to growing tissues, whereas SNF1‐related kinase 1 (SnRK1) modulates growth responses, maintaining cellular homeostasis. Innovative treatments, priming, biopriming encapsulation, have proven effective improving germination rates, tolerance vigour by optimising regulation, activation microbial interactions. Sugar‐based treatments offer substantial potential enhancing crop productivity Future research should focus on refining these strategies, exploring sugar–hormone interactions using genomic tools advance improvement. Thus, small‐molecule hold transformative sustainable agriculture, providing a route enhance technology adaptability global challenges.

Language: Английский

Citations

0
Calmodulin and calcium signaling in potato tuberization: The role of membrane transporters in stress adaptation DOI
Muthu Thiruvengadam,

Yong-Ik Jin,

Hae Won Jang

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109829 - 109829

Published: March 1, 2025

Language: Английский

Citations

0
Enhancing Abiotic Stress Resilience in Mediterranean Woody Perennial Fruit Crops: Genetic, Epigenetic, and Microbial Molecular Perspectives in the Face of Climate Change DOI Open Access
Aliki Kapazoglou, Eleni Tani, Vasileios Papasotiropoulos

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3160 - 3160

Published: March 29, 2025

Enhanced abiotic stresses such as increased drought, elevated temperatures, salinity, and extreme weather phenomena severely affect major crops in the Mediterranean area, a ‘hot spot’ of climate change. Plants have evolved mechanisms to face stressful conditions adapt environmental pressures. Intricate molecular processes involving genetic epigenetic factors plant–microbe interactions been implicated response tolerance stress. Deciphering whereby plants perceive respond stress is crucial for developing strategies counteract challenges. Progress determining genes, complex gene networks, biochemical pathways, well plant–microbiota crosstalk, involved has achieved through application tools diverse resources. This knowledge could be particularly useful accelerating plant improvement generating resilient varieties, especially concerning woody perennial crops, where classical breeding lengthy labor-intensive process. Similarly, understanding provide insights into innovative approaches facing conditions. In this review, we comprehensive overview discuss recent findings genetic, epigenetic, microbial aspects shaping responses, context enhancing resilience important fruit crops.

Language: Английский

Citations

0
The SWEET14 sugar transporter mediates mycorrhizal symbiosis and carbon allocation in Dendrobium officinale DOI Creative Commons

Liumin Li,

Xueying Wang,

Li Hua

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 2, 2025

Abstract Orchid mycorrhizal (OM) fungi form mutualistic relationships with orchids, facilitating the uptake of minerals from soil in exchange for sugars and lipids provided by plant. In this study, we explored role DoSWEET14 sugar transporter Dendrobium officinale during OM symbiosis. Transcriptome sequencing revealed significant changes gene expression roots, particularly upregulation . Subcellular localization studies confirmed that is localized to plasma membrane, suggesting its involvement nutrient between orchid fungi. Complementation assays using a yeast strain deficient hexose transport demonstrated has broad substrate specificity, efficiently transporting multiple monosaccharides. Additionally, overexpression Arabidopsis increased content without affecting biomass, supporting carbohydrate storage These findings suggest plays key regulating carbon allocation stabilizing relationship

Language: Английский

Citations

0