Extracellular Vesicles from Ocular Melanoma Have Pro-Fibrotic and Pro-Angiogenic Properties on the Tumor Microenvironment DOI Creative Commons
Léo Piquet, Kelly Coutant, Andrew K. Mitchell

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(23), P. 3828 - 3828

Published: Nov. 29, 2022

Uveal melanoma (UM) is the most common primary intraocular tumor and often spreads to liver. Intercellular communication though extracellular vesicles (EVs) plays an important role in several oncogenic processes, including metastasis, therapeutic resistance, immune escape. This study examines how EVs released by UM cells modify stellate endothelial microenvironment. The surface markers, concentration size of derived from or choroidal melanocytes were characterized high-resolution flow cytometry, electron microscopy, Western blotting. selective biodistribution was studied mice fluorescence imaging. activation/contractility tubular organization after exposure melanomic determined traction force collagen gel contraction, tube formation assays. We showed that large healthy are heterogenous size, as well their expression phosphatidylserine, tetraspanins, Tsg101. Melanomic mainly accumulated liver lungs mice. Hepatic with internalized had increased contractility, whereas EV-treated developed more capillary-like networks. Our demonstrates transfer leads a pro-fibrotic pro-angiogenic phenotype hepatic cells.

Language: Английский

Plant-Derived Extracellular Vesicles and Their Exciting Potential as the Future of Next-Generation Drug Delivery DOI Creative Commons
Faisal A. Alzahrani, Mohammad Imran Khan, Nader Kameli

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 839 - 839

Published: May 15, 2023

Plant cells release tiny membranous vesicles called extracellular (EVs), which are rich in lipids, proteins, nucleic acids, and pharmacologically active compounds. These plant-derived EVs (PDEVs) safe easily extractable have been shown to therapeutic effects against inflammation, cancer, bacteria, aging. They promise preventing or treating colitis, alcoholic liver disease, even COVID-19. PDEVs can also be used as natural carriers for small-molecule drugs acids through various administration routes such oral, transdermal, injection. The unique advantages of make them highly competitive clinical applications preventive healthcare products the future. This review covers latest methods isolating characterizing PDEVs, their disease prevention treatment, potential a new drug carrier, with special attention commercial viability toxicological profile, future nanomedicine therapeutics. champions formation task force specializing address global need rigor standardization PDEV research.

Language: Английский

Citations

56
Engineering of MSC-Derived Exosomes: A Promising Cell-Free Therapy for Osteoarthritis DOI Creative Commons
Jin Cheng, Yixin Sun, Yong Ma

et al.

Membranes, Journal Year: 2022, Volume and Issue: 12(8), P. 739 - 739

Published: July 28, 2022

Osteoarthritis (OA) is characterized by progressive cartilage degeneration with increasing prevalence and unsatisfactory treatment efficacy. Exosomes derived from mesenchymal stem cells play an important role in alleviating OA promoting regeneration, inhibiting synovial inflammation mediating subchondral bone remodeling without the risk of immune rejection tumorigenesis. However, low yield, weak activity, inefficient targeting ability unpredictable side effects natural exosomes have limited their clinical application. At present, various approaches been applied exosome engineering to regulate production function, such as pretreatment parental cells, drug loading, genetic surface modification. Biomaterials also proved facilitate efficient delivery enhance effectiveness. Here, we summarize current understanding biogenesis, isolation characterization exosomes, focus on large-scale preparation engineered well therapeutic potential OA, thus providing novel insights into exploring advanced MSC-derived exosome-based cell-free therapy for OA.

Language: Английский

Citations

40
Advances in Platelet Rich Plasma-Derived Extracellular Vesicles for Regenerative Medicine: A Systematic-Narrative Review DOI Open Access
Eduardo Anitua, María Troya, Juan Manuel Falcón‐Pérez

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13043 - 13043

Published: Aug. 22, 2023

The use of platelet-rich plasma (PRP) has gained increasing interest in recent decades. platelet secretome contains a multitude growth factors, cytokines, chemokines, and other biological biomolecules. In years, developments the field platelets have led to new insights, attention been focused on platelets’ released extracellular vesicles (EVs) their role intercellular communication. this context, aim review was compile current evidence PRP-derived identify advantages limitations fortheir upcoming clinical applications. A total 172 articles were identified during systematic literature search through two databases (PubMed Web Science). Twenty publications met inclusion criteria included review. According results, PRP-EVs clinic is an emerging great that represents promising therapeutic option, as efficacy demonstrated majority fields applications However, lack standardization along procedures both PRP EVs makes it extremely challenging compare results among studies. Establishing standardized conditions ensure optimized detailed protocols define parameters such dose or EV origin therefore urgent. Further studies elucidate real contribution terms composition functionality should also be performed. Nevertheless, research provides novel basis deal with regenerative medicine drug delivery future.

Language: Английский

Citations

29
Diverse drug delivery systems for the enhancement of cancer immunotherapy: an overview DOI Creative Commons
Xu Liu, Cheng Yang,

Yao Mu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 17, 2024

Despite the clear benefits demonstrated by immunotherapy, there is still an inevitable off-target effect resulting in serious adverse immune reactions. In recent years, research and development of Drug Delivery System (DDS) has received increased prominence. decades development, DDS ability to deliver drugs a precisely targeted manner mitigate side effects advantages flexible control drug release, improved pharmacokinetics, distribution. Therefore, we consider that combining cancer immunotherapy with can enhance anti-tumor ability. this paper, provide overview latest delivery strategies briefly introduce characteristics based on nano-carriers (liposomes, polymer nano-micelles, mesoporous silica, extracellular vesicles, etc.) coupling technology (ADCs, PDCs protein degradation). Our aim show readers variety platforms under different mechanisms, analyze their limitations, more superior accurate targeting for immunotherapy.

Language: Английский

Citations

16
Extracellular vesicles as carriers of mRNA: Opportunities and challenges in diagnosis and treatment DOI Creative Commons

R.D. Kirian,

Darby Steinman,

Christopher M. Jewell

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(5), P. 2265 - 2289

Published: Jan. 1, 2024

Extracellular vesicles (EVs) are produced by all cells in the body. These biological nanoparticles facilitate cellular communication through transport of diverse cargoes, including small molecules, proteins, and nucleic acids. mRNA cargoes have gained particular interest given their role translation functional proteins. As a biomarker platform, EVs can be found nearly biofluids-blood, mucus, urine, cerebrospinal fluid, saliva-providing real-time insight into parent cell tissue function. mRNAs carried protected from degradation, resulting improved detection compared to free mRNA, recent work demonstrates promising results using these as biomarkers for cancer, neurological diseases, infectious gynecologic obstetric outcomes. Furthermore, innate cargo carrying, targeting, barrier crossing abilities EVs, structures been proposed therapeutic carriers mRNA. Recent advances demonstrate methods loading range disease indications. Here, we review studies diagnostics therapeutics. We discuss challenges associated with diagnostic applications highlight opportunities future development.

Language: Английский

Citations

9
Exosome Source Matters: A Comprehensive Review from the Perspective of Diverse Cellular Origins DOI Creative Commons
Y Chen, Weili Qi, Zhenghao Wang

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 147 - 147

Published: Jan. 22, 2025

Exosomes have emerged as promising therapeutic agents in regenerative medicine. This review introduces a novel cell type-oriented perspective to systematically analyze exosomal properties therapies. To our knowledge, this is the first comprehensively compare exosomes based on cellular source type, offering unprecedented insights into selecting optimal exosome producers for targeted applications. Factors beyond origin influencing efficacy, such donor sites and collection methods, are also explored here. By synthesizing key advances, we propose research directions end. We aim accelerate development of more effective exosome-based therapies highlight underexplored rapidly evolving field.

Language: Английский

Citations

1
Scalable production and purification of engineered ARRDC1-mediated microvesicles in a HEK293 suspension cell system DOI Creative Commons

Kristin Luther,

Ali Navaei,

Leah Gens

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 1, 2025

Abstract Engineering of human ARRDC1-mediated microvesicles (ARMMs) as non-viral vehicles for delivery gene therapies bears the potential to enable novel therapeutic paradigms. We evaluated two scalable strategies generate ARMMs loaded with protein cargo, by transient transfection or stable cell line-based production. The upstream production processes utilized a suspension-adapted HEK293-derived line, termed 5B8. 5B8 cells yielded robust after loading construct using line containing transgene that encodes cassette, in shake flasks stirred tank bioreactor, respectively. were purified ultracentrifugation (small scale) combination TFF and AEX (scalable production). Both purification methods produced comparable ARMMs, terms size payload incorporation. Single particle analysis showed approximately 50% payload-containing ARMMs. Additionally, an vivo study was conducted mice investigate half-life biodistribution administered intravenously. rapid predominantly spleen liver and, lesser extent, kidneys, lungs. plasma 6 ± 0.4 min. Altogether, this work advances knowledge on scale-up engineered cell-derived vesicles future molecules.

Language: Английский

Citations

1
Emerging extracellular vesicle-based carriers for glioblastoma diagnosis and therapy DOI
Jingjing Wang, Yue Liu, Fengbo Liu

et al.

Nanoscale, Journal Year: 2023, Volume and Issue: 15(26), P. 10904 - 10938

Published: Jan. 1, 2023

This review summarizes recent advances in extracellular vesicle-based carriers for glioblastoma diagnosis and therapy, points out the current challenges perspectives.

Language: Английский

Citations

18
Exosomes in transplantation: Role in allograft rejection, diagnostic biomarker, and therapeutic potential DOI
Prathab Balaji Saravanan, Jagan Kalivarathan,

Faizaan Khan

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 324, P. 121722 - 121722

Published: April 24, 2023

Language: Английский

Citations

17
Advances in 3D Culture Models to Study Exosomes in Triple-Negative Breast Cancer DOI Open Access

Neelum Aziz Yousafzai,

Lamyae El Khalki, Wei Wang

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(5), P. 883 - 883

Published: Feb. 22, 2024

Breast cancer, a leading cause of cancer-related deaths globally, exhibits distinct subtypes with varying pathological, genetic, and clinical characteristics. Despite advancements in breast cancer treatments, its histological molecular heterogeneity pose significant challenge. Triple-negative (TNBC), highly aggressive subtype lacking targeted therapeutics, adds to the complexity treatment. Recent years have witnessed development advanced 3D culture technologies, such as organoids spheroids, providing more representative models healthy human tissue various malignancies. These structures, resembling organs structure function, are generated from stem cells or organ-specific progenitor via self-organizing processes. Notably, systems bridge gap between 2D cultures vivo studies, offering accurate representation tumors’ Exosomes, small nano-sized molecules secreted by stromal/cancer-associated fibroblast cells, garnered attention. They play crucial role cell-to-cell communication, influencing tumor progression, invasion, metastasis. The environment enhances exosome efficiency compared traditional cultures, impacting transfer specific cargoes therapeutic effects. Furthermore, exosomes shown promise improving outcomes, acting potential vehicles for treatment administration. Studies demonstrated their pro-angiogenesis innate mimicking cellular therapies without side model holds addressing challenges associated drug resistance, insights into mechanisms underlying multidrug resistance serving platform screening. This review seeks emphasize studying especially understanding involvement pathology.

Language: Английский

Citations

7