TET1-Lipid Nanoparticle Encapsulating Morphine for Specific Targeting of Peripheral Nerve for Pain Alleviation

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 4759 - 4777

Published: May 1, 2024

Background: Opioids are irreplaceable analgesics owing to the lack of alternative that offer opioid-like pain relief. However, opioids have many undesirable central side effects. Restricting peripheral opioid receptors could reduce those effects while maintaining analgesia. Methods: To achieve this goal, we developed Tet1-LNP (morphine), a neural-targeting lipid nanoparticle encapsulating morphine specifically activate receptor in dorsal root ganglion (DRG) and significantly caused by activation brain. (morphine) were successfully prepared using thin-film hydration method. In vitro, uptake was assessed differentiated neuron-like PC-12 cells primary cells. The DRGs brain vivo. Von Frey filament Hargreaves tests used assess antinociception chronic constriction injury (CCI) neuropathic model. Morphine concentration blood evaluated ELISA. Results: had an average size 131 nm. showed high cellular targeted DRG vitro. CCI mice treated with experienced prolonged analgesia for nearly 32 h compared 3 free ( p < 0.0001). Notably, group eight-fold lower than Conclusion: Our study presents system neural delivery morphine. We anticipate will safe formulation treatment, promise further development clinical applications. Keywords: nanoparticle, Tet1, nerve-targeted, management,

Language: Английский

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scSemiProfiler: Advancing large-scale single-cell studies through semi-profiling with deep generative models and active learning

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 16, 2024

Abstract Single-cell sequencing is a crucial tool for dissecting the cellular intricacies of complex diseases. Its prohibitive cost, however, hampers its application in expansive biomedical studies. Traditional deconvolution approaches can infer cell type proportions from more affordable bulk data, yet they fall short providing detailed resolution required single-cell-level analyses. To overcome this challenge, we introduce “scSemiProfiler”, an innovative computational framework that marries deep generative models with active learning strategies. This method adeptly infers single-cell profiles across large cohorts by fusing data targeted few rigorously chosen representatives. Extensive validation heterogeneous datasets verifies precision our semi-profiling approach, aligning closely true profiling and empowering refined Originally developed extensive disease cohorts, “scSemiProfiler” adaptable broad applications. It provides scalable, cost-effective solution profiling, facilitating in-depth investigation various biological domains.

Language: Английский

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Sweet science: Exploring the impact of fructose and glucose on brown adipocyte differentiation using optical diffraction tomography

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 4, 2024

ABSTRACT The thermogenic capacity of brown adipose tissue (BAT) has garnered much attention for its potential to regulate systemic energy balance. BAT depot size and function need be tightly prevent loss metabolic homeostasis due dissipation via non-shivering thermogenesis. While adipocyte-intrinsic mechanisms controlling thermogenesis are critical, an increasing appreciation the role microenvironment is emerging. For example, changes in circulating hexoses dietary intake have shown impact function. Here, we show that murine preadipocytes metabolism impacted when fructose used as sole carbon source. Similarly differentiation medium containing only yield mature adipocytes with fewer lipid droplets, a concomitant decrease adipogenic genes. These deficiencies also observed human preadipocytes, where cutting-edge optical imaging modalities total cell mass fructose-only medium. Taken together, significantly impacts growth function, implications diets potentially mitigating efficacy BAT-targeted therapies.

Language: Английский

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Biomaterials Science 3D and 4D Bioprinting submission

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(23), P. 6047 - 6062

Published: Jan. 1, 2024

The myotendinous junction (MTJ) facilitates force transmission between muscle and tendon to produce joint movement. complex microarchitecture regional mechanical heterogeneity of the pose major challenges in creating this interface

Language: Английский

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Bioconversion of snakeskin shed into bioactive hydrolysate possessing antioxidant and cell proliferative potential

Process Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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