Journal of Biophotonics,
Journal Year:
2023,
Volume and Issue:
17(2)
Published: Nov. 17, 2023
We
investigated
the
effects
of
low-level
Er:YAG
laser
irradiation
on
proliferation
and
alternations
in
early
gene
expression
gingival
fibroblasts.
Mice
primary
fibroblasts
were
irradiated
with
an
(1.8,
3.9,
5.8
J/cm2).
Irradiation
at
3.9
J/cm2
promoted
cell
without
significant
changes
lactate
dehydrogenase
or
Hspa1a
expression.
Three
hours
after
J/cm2,
Fn1
level
was
significantly
increased.
RNA-seq
identified
15
differentially
expressed
genes
between
non-irradiated
cells,
some
which
belonged
to
immediate
(IEGs).
Kyoto
Encyclopedia
Genes
Genomes
pathway
analysis
indicated
MAPK
enhancement,
set
enrichment
showed
TGF-β
signaling
set.
Enhanced
via
disappeared
upon
inhibition
Dusp4,
Dusp5,
Tgfr1
Low-level
irradiation,
especially
a
major
temperature
elevation,
enhanced
fibroblast
proliferation,
following
IEG
Experimental and Therapeutic Medicine,
Journal Year:
2024,
Volume and Issue:
28(1)
Published: May 22, 2024
Phenytoin
(PHT)‑induced
gingival
overgrowth
is
caused
by
the
increased
proliferation
and
reduced
apoptosis
of
fibroblasts
in
inflammatory
gingiva.
Licorice
has
long
been
used
as
a
component
therapeutic
preparations.
It
inhibits
cell
proliferation,
induces
anti‑inflammatory
effects.
18‑α‑glycyrrhetinic
acid
(18α‑GA),
active
compound
licorice,
promotes
various
types
cells.
The
present
study
determined
whether
18α‑GA
affects
exposed
to
PHT.
aimed
establish
basis
for
application
treat
induced
Human
from
healthy
donors
were
cultured
semi‑confluence
then
stimulated
serum‑free
DMEM
containing
PHT
with
or
without
subsequent
experiments.
Apoptotic
cells
detected
ELISA.
Analysis
distribution
cycle
phases
apoptotic
population
was
performed
flow
cytometry.
expression
levels
mRNAs
proteins
regulators
measured
using
reverse
transcription‑quantitative
PCR
western
blotting,
respectively.
Caspase
(CASP)
activities
assessed
an
Treatment
markedly
number
cells,
BCL2
mRNA
expression,
CASP2
receptor
(TNFRSF)‑interacting
serine‑threonine
kinase
1
(RIPK1)
domain
adaptor
death
domain,
Fas
(TNFRSF6)‑associated
via
RIPK1,
tumor
necrosis
factor
superfamily;
member
1A,
TNF
receptor‑associated
2,
CASP2,
CASP3
CASP9
also
upregulated
protein
caspase‑2,
caspase‑3
caspase‑9.
These
results
demonstrated
that
through
activation
pathways
signaling
pathway
treated
exhibited
potential
treatment
PHT‑induced
overgrowth.
International Journal of Nanomedicine,
Journal Year:
2024,
Volume and Issue:
Volume 19, P. 11941 - 11956
Published: Nov. 1, 2024
Periodontitis
is
a
chronic
inflammatory
oral
disease
that
causes
defects
in
periodontal
tissue.
Conventional
therapies
are
limited,
and
often
lead
to
high
recurrence
rates.
The
emerging
concept
of
medicinal
food
homology
has
shed
light
on
the
potential
ginger
as
therapeutic
adjuvant
for
periodontitis,
given
its
antioxidant
anti-inflammatory
properties.
However,
fresh
exhibits
poor
stability
bioavailability.
Ginger
exosome-like
nanoparticles
(GELNs),
derivative
ginger,
have
not
been
reported
exert
effects
periodontitis.
This
study
aimed
explore
GELNs
tissue
damage
caused
by
periodontitis
their
underlying
mechanisms
action.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Dec. 5, 2024
Periodontal
disease
(PD)
is
a
chronic
inflammatory
condition
that
affects
the
teeth
and
their
supporting
tissues,
ultimately
culminating
in
tooth
loss.
Currently,
treatment
modalities,
such
as
systemic
local
administration
of
antibiotics,
serve
to
mitigate
progression
inflammation
yet
fall
short
restoring
original
anatomical
structure
physiological
function
periodontal
tissues.
Biocompatible
material-based
tissue
engineering
seems
be
promising
therapeutic
strategy
for
treating
PD.
Collagen,
component
extracellular
matrix
commonly
used
engineering,
has
been
regarded
biogenic
material
regeneration
owing
its
high
cell-activating
biocompatible
properties.
The
structural
chemical
similarities
between
collagen
components
oral
render
it
candidate
dental
regeneration.
This
review
explored
properties
current
applications
We
also
discussed
recent
therapies
preparation
techniques.
scrutinizes
pros
cons
associated
with
application
collagen-based
biomaterials
PD
treatment,
aiming
pave
way
future
management
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(13), P. 10811 - 10811
Published: June 28, 2023
It
has
been
shown
that
synovial
fibroblasts
(SF)
play
a
key
role
in
the
initiation
of
inflammation
and
joint
destruction,
leading
to
arthritis
progression.
Fibroblasts
may
express
major
histocompatibility
complex
class
II
region
(MHCII)
molecules,
thus,
they
could
be
able
process
present
antigens
immunocompetent
cells.
Here
we
examine
whether
different
types
(synovial,
dermal,
thymic
murine
fibroblasts,
destructive
LS48
noninvasive
NIH/3T3
fibroblasts)
involved
rheumatoid
(RA)
pathogenesis
can
type
collagen
(COL2)-an
autoantigen
associated
with
RA.
Using
panel
MHCII/Aq-restricted
T-cell
hybridoma
lines
specifically
recognize
an
immunodominant
COL2
epitope
(COL2259-273),
found
activate
several
clones
posttranslationally
glycosylated
or
hydroxylated
COL2259-273
epitope.
The
HCQ.3
hybridoma,
which
is
specific
for
259-273
(Gal264),
showed
strongest
response.
Interestingly,
cells,
but
not
synovial,
were
stimulate
other
COL2-specific
hybridomas.
Our
experiments
revealed
are
hybridomas
even
absence
modified
peptide.
mechanism
this
unusual
activation
contact-dependent
involves
receptor
(TCR)
complex.
Journal of Biophotonics,
Journal Year:
2023,
Volume and Issue:
17(2)
Published: Nov. 17, 2023
We
investigated
the
effects
of
low-level
Er:YAG
laser
irradiation
on
proliferation
and
alternations
in
early
gene
expression
gingival
fibroblasts.
Mice
primary
fibroblasts
were
irradiated
with
an
(1.8,
3.9,
5.8
J/cm2).
Irradiation
at
3.9
J/cm2
promoted
cell
without
significant
changes
lactate
dehydrogenase
or
Hspa1a
expression.
Three
hours
after
J/cm2,
Fn1
level
was
significantly
increased.
RNA-seq
identified
15
differentially
expressed
genes
between
non-irradiated
cells,
some
which
belonged
to
immediate
(IEGs).
Kyoto
Encyclopedia
Genes
Genomes
pathway
analysis
indicated
MAPK
enhancement,
set
enrichment
showed
TGF-β
signaling
set.
Enhanced
via
disappeared
upon
inhibition
Dusp4,
Dusp5,
Tgfr1
Low-level
irradiation,
especially
a
major
temperature
elevation,
enhanced
fibroblast
proliferation,
following
IEG
