Effect of cellular senescence on the response of human peritoneal mesothelial cells to TGF-β DOI Creative Commons
Edyta Kawka, Rebecca Herzog, Marcin Ruciński

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 26, 2024

Abstract Transforming growth factor β (TGF-β) is implicated in both mesothelial-to-mesenchymal transition (MMT) and cellular senescence of human peritoneal mesothelial cells (HPMCs). We previously showed that senescent HPMCs could spontaneously acquire some phenotypic features MMT, which young were induced by TGF-β. Here, we used electron microscopy, as well global gene protein profiling to assess detail how exposure TGF-β impacts on vitro. found structural changes consistent with MMT young, but not HPMCs. Of all genes proteins identified reliably across treatments states, 4,656 targets represented overlapping proteins. Following TGF-β, 137 46 transcripts significantly changed cells, compared 225 only 2 cells. Identified differences between related predominantly wound healing, integrin-mediated signalling, production proteases extracellular matrix components, cytoskeleton structure. Thus, the response differs or less pronounced As a result, character magnitude postulated contribution TGF-β-induced remodelling may change cell senescence.

Language: Английский

Senescence-Induced Atherosclerosis: The Potency of Senolytic Therapy DOI Open Access

Udani Sari Ratih,

Febriana Catur Iswanti

Bioscientia Medicina Journal of Biomedicine and Translational Research, Journal Year: 2024, Volume and Issue: 8(8), P. 4682 - 4696

Published: May 20, 2024

The aging process is an inevitable occurrence that involves physiological changes at the cellular level. presence of intrinsic and extrinsic stressors can cause damage, leading to senescence premature aging. Senescent cells undergo activation p53/p21 p16INK4a pathways, induce cell cycle arrest, increased expression senescence-associated beta-galactosidase (SA-β-Gal), secretion SASP (senescence-associated secretory phenotype), "inflamm-aging" or chronic inflammation associated with senescence. These “inflamm-aging” accelerates age-related diseases, one which atherosclerosis. relationship between aging, senescence, atherosclerosis has been a focus research on pathogenesis, prevention, therapy. Recent emphasized crucial role senolytics, compounds agents capable eliminating senescent cells, in inhibiting progression slowing down Obtaining more comprehensive understanding processes effectiveness senolytics should facilitate development potent medicines mitigate side effects management cardiovascular disease extend longevity.

Language: Английский

Citations

1
Treatment of Rheumatoid Arthritis Based on the Inherent Bioactivity of Black Phosphorus Nanosheets DOI Creative Commons

Cheng Zhuang,

Ruiqi Sun,

Yuchen Zhang

et al.

Aging and Disease, Journal Year: 2024, Volume and Issue: unknown, P. 0 - 0

Published: Jan. 1, 2024

Rheumatoid arthritis (RA) is an autoimmune disease that affects the living quality of patients, especially elderly population. RA-related morbidity and mortality increase significantly with age, while current clinical drugs for RA are far from satisfactory may have serious side effects. Therefore, development new higher biosafety efficacy demanding. Black phosphorus nanosheets (BPNSs) been widely studied because their excellent biocompatibility. Here, we focus on inherent bioactivity BPNSs, report potential BPNSs as a therapeutic drug elucidate underlying mechanism. We find inhibit autophagy at early stage via AMPK-mTOR pathway, switch energy metabolic pathway to oxidative phosphorylation, intracellular ATP levels, suppress apoptosis, reduce inflammation stress, down-regulate senescence-associated secretory phenotype (SASP)-related genes in rheumatoid synovial fibroblasts (RA-SFs). Further, induce apoptosis macrophages promote transition M1 M2 by regulating related cytokines. Significantly, administration can alleviate key pathological features mice, revealing great potential. This study provides novel option treating RA, emerging promising candidate.

Language: Английский

Citations

1
The role of CYP-sEH derived lipid mediators in regulating mitochondrial biology and cellular senescence: implications for the aging heart DOI Creative Commons
Ala Yousef, Liye Fang, Mohammad Heidari

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 5, 2024

Cellular senescence is a condition characterized by stable, irreversible cell cycle arrest linked to the aging process. The accumulation of senescent cells in cardiac muscle can contribute various cardiovascular diseases (CVD). Telomere shortening, epigenetic modifications, DNA damage, mitochondrial dysfunction, and oxidative stress are known contributors onset cellular heart. link between processes contributed age-related decline function. These include changes functions behaviours that arise from factors, including impaired dynamics, dysregulated biogenesis, mitophagy, (mtDNA), reduced respiratory capacity, structural changes. Thus, regulation biology has role function hearts. Targeting may provide novel therapeutic approach for treating preventing CVD associated with aging. CYP epoxygenases metabolize N-3 N-6 polyunsaturated fatty acids (PUFA) into epoxylipids readily hydrolyzed diol products soluble epoxide hydrolase (sEH). Increasing levels or inhibition sEH demonstrated protective effects Evidence suggests they play regulating mitochondria, thus reducing adverse In this review, we discuss how mitochondria induce affect process aged

Language: Английский

Citations

1
Unlocking the dark matter: noncoding RNAs and RNA modifications in cardiac aging DOI
Jiayi Kang, James Rhee,

Chunyan Wang

et al.

AJP Heart and Circulatory Physiology, Journal Year: 2024, Volume and Issue: 326(3), P. H832 - H844

Published: Feb. 2, 2024

Cardiac aging is a multifaceted process that encompasses structural and functional alterations culminating in heart failure. As the elderly population continues to expand, there growing urgent need for interventions combat age-related cardiac decline. Noncoding RNAs have emerged as critical regulators of cellular biochemical processes underlying disease. This review summarizes our current understanding how noncoding function during aging, with particular emphasis on mechanisms RNA modification control their activity. Targeting potential novel therapeutics also discussed.

Language: Английский

Citations

0
Effect of cellular senescence on the response of human peritoneal mesothelial cells to TGF-β DOI Creative Commons
Edyta Kawka, Rebecca Herzog, Marcin Ruciński

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 26, 2024

Abstract Transforming growth factor β (TGF-β) is implicated in both mesothelial-to-mesenchymal transition (MMT) and cellular senescence of human peritoneal mesothelial cells (HPMCs). We previously showed that senescent HPMCs could spontaneously acquire some phenotypic features MMT, which young were induced by TGF-β. Here, we used electron microscopy, as well global gene protein profiling to assess detail how exposure TGF-β impacts on vitro. found structural changes consistent with MMT young, but not HPMCs. Of all genes proteins identified reliably across treatments states, 4,656 targets represented overlapping proteins. Following TGF-β, 137 46 transcripts significantly changed cells, compared 225 only 2 cells. Identified differences between related predominantly wound healing, integrin-mediated signalling, production proteases extracellular matrix components, cytoskeleton structure. Thus, the response differs or less pronounced As a result, character magnitude postulated contribution TGF-β-induced remodelling may change cell senescence.

Language: Английский

Citations

0