Free Radical Biology and Medicine,
Journal Year:
2024,
Volume and Issue:
222, P. 259 - 274
Published: June 20, 2024
The
skin
is
made
up
of
different
layers
with
various
gradients,
which
maintain
a
complex
microenvironment,
particularly
in
terms
oxygen
levels.
However,
all
types
cells
are
cultured
conventional
incubators
that
do
not
reproduce
physiological
Instead,
they
at
atmospheric
levels,
condition
far
removed
from
physiology
and
may
lead
to
the
generation
free
radicals
known
induce
ageing.
This
review
aims
summarize
current
literature
on
effect
levels
cells,
highlight
shortcomings
vitro
models,
demonstrate
importance
respecting
We
begin
by
clarifying
terminology
used
about
describe
specific
distribution
skin.
discuss
how
adapt
their
consumption
metabolism
environment,
as
well
changes
induced,
particularly,
redox
state,
life
cycle
functions.
examine
effects
both
simple
culture
models
more
reconstructed
models.
Finally,
we
present
implications
modulation
for
therapeutic
approach.
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: July 26, 2024
Mesenchymal
stromal
stem
cells
(MSCs)
possess
a
remarkable
potential
for
numerous
clinical
applications
due
to
their
unique
properties
including
self-renewal,
immunomodulation,
paracrine
actions
and
multilineage
differentiation.
However,
the
translation
of
MSC-based
Advanced
Therapy
Medicinal
Products
(ATMPs)
into
clinic
has
frequently
met
with
inconsistent
outcomes.
One
suspected
reasons
this
issue
is
inherent
extensive
variability
that
exists
among
such
ATMPs,
which
makes
interpretation
efficacy
difficult
assess,
as
well
compare
results
various
studies.
This
stems
from
differences
in
tissue
sources,
donor
attributes,
variances
manufacturing
protocols,
modes
administration.
MSCs
can
be
isolated
tissues
bone
marrow,
umbilical
cord,
adipose
others,
each
its
phenotypic
functional
characteristics.
While
different
sources
do
share
common
features,
they
also
exhibit
distinct
gene
expression
profiles
properites.
Donor-specific
factors
age,
sex,
body
mass
index,
underlying
health
conditions
influence
MSC
phenotype,
morphology,
differentiation
function.
Moreover,
variations
preparation
products
introduces
additional
heterogeneity
result
cell
culture
media
composition,
presence
or
absence
added
growth
factors,
use
serum
supplements
culturing
techniques.
Once
are
formulated,
storage
protocols
play
pivotal
role
efficacy.
Factors
affect
viability
include
concentration,
delivery
solution
importantly,
post-thawing
where
applicable.
Ensuing,
administration
critically
distribution
functionallity
administered
cells.
As
therapies
continue
advance
through
trials,
implication
strategies
reduce
product
imperative.
Central
addressing
these
challenges
need
precise
prediction
responses,
require
well-defined
populations
harmonized
assessment
specific
functions.
By
issues
by
meaningful
approaches,
as,
e.g.,
pooling,
field
overcome
barriers
towards
more
consistent
effective
therapies.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(13), P. 10620 - 10620
Published: June 25, 2023
Cancer
is
intrinsically
complex,
comprising
both
heterogeneous
cellular
composition
and
extracellular
matrix.
In
vitro
cancer
research
models
have
been
widely
used
in
the
past
to
model
study
cancer.
Although
two-dimensional
(2D)
cell
culture
traditionally
for
research,
they
many
limitations,
such
as
disturbance
of
interactions
between
environments
changes
morphology,
polarity,
division
mechanism,
differentiation
motion.
Moreover,
2D
are
usually
monotypic.
This
implies
that
tumor
ineffective
at
accurately
recapitulating
complex
aspects
growth,
well
their
radiation
responses.
Over
decade
there
has
significant
uptake
three-dimensional
(3D)
by
researchers,
highlighting
a
complementary
studies
effects
on
tumors,
especially
conjunction
with
chemotherapy.
The
introduction
3D
approaches
aims
vivo
tissue
positioning
itself
halfway
animal
models,
thus
opening
up
new
possibilities
response
mechanisms
healthy
tissues.
International Journal of Nanomedicine,
Journal Year:
2024,
Volume and Issue:
Volume 19, P. 4357 - 4375
Published: May 1, 2024
Abstract:
Wound
healing
is
a
sophisticated
and
orderly
process
of
cellular
interactions
in
which
the
body
restores
tissue
architecture
functionality
following
injury.
Healing
chronic
diabetic
wounds
difficult
due
to
impaired
blood
circulation,
reduced
immune
response,
disrupted
repair
mechanisms,
are
often
associated
with
diabetes.
Stem
cell-derived
extracellular
vesicles
(SC-EVs)
hold
regenerative
potential,
encapsulating
diverse
cargo
proteins,
RNAs,
cytokines,
presenting
safe,
bioactivity,
less
ethical
issues
than
other
treatments.
SC-EVs
orchestrate
multiple
processes
by
modulating
communication,
increasing
angiogenesis,
promoting
recruitment
differentiation
progenitor
cells,
thereby
potentiating
reparative
milieu
for
wound
healing.
Therefore,
this
review
investigated
effects
mechanisms
EVs
from
various
stem
cells
healing,
as
well
their
limitations
challenges.
Continued
exploration
has
potential
revolutionize
care.
Keywords:
diabetes,
EVs,
ncRNAs,
regeneration
Journal of Experimental Zoology Part B Molecular and Developmental Evolution,
Journal Year:
2024,
Volume and Issue:
342(3), P. 301 - 312
Published: Jan. 8, 2024
Abstract
In
vitro
assays
are
crucial
tools
for
gaining
detailed
insights
into
various
biological
processes,
including
metabolism.
Cave
morphs
of
the
river‐dwelling
fish
species,
Astyanax
mexicanus
,
have
adapted
their
metabolism
allowing
them
to
thrive
in
biodiversity‐deprived
and
nutrient‐limited
environment
caves.
Liver‐derived
cells
from
cave
river
A.
proven
be
excellent
resources
better
understand
unique
these
fish.
However,
current
2D
cultures
not
fully
captured
complex
metabolic
profile
liver.
It
is
known
that
3D
culturing
can
modulate
transcriptomic
state
when
compared
its
monolayer
culture.
Therefore,
broaden
possibilities
system
by
modeling
a
wider
gamut
pathways,
we
cultured
liver‐derived
both
surface
cavefish
spheroids.
We
successfully
established
at
cell
seeding
densities
several
weeks
characterized
resultant
variations.
found
exhibit
an
altered
consequently
represent
range
cycle
changes
antioxidant
activities,
associated
with
liver
functioning
as
Enzymatic
assay
measuring
antioxidants
culture
spheroids
also
revealed
enhanced
antioxidative
capacity
spheroids,
line
differential
gene
expression
data.
Additionally,
exhibited
cave‐specific
signatures,
making
it
suitable
evolutionary
studies
adaptation.
Notably,
derived
enriched
genes
responding
xenobiotic
stimulus,
while
ones
immune
response,
which
resonated
physiologically
adaptations
each
morph.
Taken
together,
prove
promising
model
widening
our
understanding
vertebrates
general.
Stem Cells International,
Journal Year:
2024,
Volume and Issue:
2024(1)
Published: Jan. 1, 2024
Chronic
nonhealing
wounds
significantly
reduce
patients'
quality
of
life
and
are
a
major
burden
on
healthcare
systems.
Over
the
past
few
decades,
tissue
engineering
materials
have
emerged
as
viable
option
for
wound
healing,
with
cell-derived
extracellular
matrix
(CDM)
showing
remarkable
results.
The
CDM's
compatibility
resemblance
to
natural
microenvironment
confer
distinct
advantages
tissue-engineered
scaffolds
in
repair.
This
review
summarizes
current
processes
CDM
preparation,
various
cell
decellularization
protocols,
common
characterization
methods.
Furthermore,
it
discusses
applications
including
skin
defect
repair,
angiogenesis,
engineered
vessels,
offers
perspectives
future
developments.
Materials Today Bio,
Journal Year:
2023,
Volume and Issue:
20, P. 100686 - 100686
Published: June 1, 2023
Umbilical
cord-derived
mesenchymal
stem
cells
(UC-MSC)
are
promising
candidates
for
wound
healing.
However,
the
low
amplification
efficiency
of
MSC
in
vitro
and
their
survival
rates
after
transplantation
have
limited
medical
application.
In
this
study,
we
fabricated
a
micronized
amniotic
membrane
(mAM)
as
microcarrier
to
amplify
used
mAM
(mAM-MSC)
complexes
repair
burn
wounds.
Results
showed
that
could
live
proliferate
on
3D
culture
system,
exhibiting
higher
cell
activity
than
2D
culture.
Transcriptome
sequencing
expression
growth
factor-related,
angiogenesis-related,
healing-related
genes
was
significantly
upregulated
mAM-MSC
compared
traditional
2D-cultured
MSC,
which
verified
via
RT-qPCR.
Gene
ontology
(GO)
analysis
differentially
expressed
(DEGs)
significant
enrichment
terms
related
proliferation,
angiogenesis,
cytokine
activity,
healing
mAM-MSC.
model
C57BL/6J
mice,
topical
application
accelerated
injection
alone
accompanied
by
longer
greater
neovascularization
wound.
Bioengineering,
Journal Year:
2025,
Volume and Issue:
12(3), P. 245 - 245
Published: Feb. 28, 2025
Here,
we
present
a
novel
3D
bioprinted
model
of
the
forebrain
cortex
designed
to
quantify
neurite
outgrowth
across
hydrogel
bridge.
To
validate
this
model,
cultured
Alzheimer’s
disease
(AD)
cortical
populations
derived
from
human
iPSCs
carrying
APP
(amyloid
precursor
protein)
mutations
(K670M/N671L
+
V717F).
Neurite
and
synapse
formation
were
significantly
impaired
in
AD
mutant
cultures
compared
controls,
but
was
not
replicated
2D,
highlighting
deficits
these
traditional
2D
cell
culture
models.
investigate
mechanisms
underlying
models
AD,
assessed
amyloid-β
dysfunction,
mitochondrial
health,
oxidative
stress
both
conditions.
In
exhibited
reduced
membrane
potential
fragmented
networks,
indicating
dysfunction
cellular
energy
deficits.
Additionally,
elevated
proteostasis
disruption
identified
as
indicators
damage,
which
may
be
limiting
extension.
Furthermore,
transcriptomic
(bulk
RNA-Seq)
analysis
revealed
distinct
differences
gene
expression
pathways
between
suggesting
alternate
pathology
This
study
demonstrates
functionality
for
quantifying
connectivity
identifying
mechanisms.
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
13
Published: March 13, 2025
Human
neural
stem/progenitor
cells
(hNSCs)
can
potentially
treat
neurological
diseases,
but
their
low
survival
and
proliferation
rates
after
transplantation
remain
challenging.
In
our
study,
we
preincubated
hNSCs
with
the
human
cerebrospinal
fluid
(CSF)
to
obtain
closer
physiological
brain
environment
assess
NSC
fate
therapeutic
abilities
in
vitro,
ex
vivo,
vivo.
We
observed
significant
changes
differentiation,
migratory,
secretory
potential
of
CSF-treated
hNSCs,
as
well
elevated
neuroprotective
co-culture
ischemically
damaged
by
oxygen-glucose
deprivation
(OGD)
organotypic
rat
hippocampal
slices
culture
(OHC)
comparison
cultured
standard
conditions.
Next,
investigated
anti-inflammatory
an
vivo
ouabain-induced
stroke
model.
This
study
highlighted
confirmed
critical
importance
nutritional
supplementation
maintaining
enhancing
its
properties.