Radiation evaluation assay using a human three-dimensional oral cancer model for clinical radiation therapy.

Talanta Open, Journal Year: 2024, Volume and Issue: 9, P. 100297 - 100297

Published: Feb. 16, 2024

With the development of various radiation-based cancer therapies, radiobiological evaluation methods instead traditional clonogenic assays with monolayer single cell culture are required to bridge gaps in clinical data. Heterogeneity within tissues is reason for bridging gap between basic and research radiotherapy. To solve this problem, we investigated an assay using a three-dimensional (3D) model tissue. In study, 3D consisting tumor stromal layers was used compare verify effects conventional two-dimensional (2D) methods. A significant difference response radiation observed 2D models. The relative number cells decreased X-ray dose escalations contrast, normal quite different Considering ability recover from radiation-induced damage, histological results were reflected Histopathological analysis potential method evaluating on margins.

Language: Английский

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Advances in skin-mimetic platforms: A comprehensive review of drug permeation models

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 98, P. 105887 - 105887

Published: June 15, 2024

Language: Английский

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3D Bioprinted Head and Neck Squamous Cell Carcinoma (HNSCC) Model Using Tunicate Derived Nanocellulose (NC) Bioink

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Head and neck squamous cell carcinoma (HNSCC) are invasive solid tumors accounting for high mortality. To improve the clinical outcome, a better understanding of tumor its microenvironment (TME) is crucial. Three ‐dimensional (3D) bioprinting emerging as powerful tool recreating TME in vitro. establish long‐term HNSCC bioprinted constructs personalized drug‐testing, this proof‐of‐principle study aims to compare two different innovative tunicate‐derived nanocellulose (NC) hydrogels against widely used semi‐synthetic gelatin methacryloyl (GelMA). Cell lines origin sites printed TEMPO Carboxy‐NC, GelMA alginate (GelMAA). Both NC show higher bioprintability than GelMAA. Carboxy‐NC supported survival, proliferation, maintenance epithelial phenotype 3D similar The hydrogel microstructure revealed differences pore size. Importantly, established model allowed testing radiochemotherapy (RCT) both patient‐derived cultures. Compared spheroid model, cytotoxic effects less, reflecting response patients. findings indicate that viable alternative gelatin‐based bioink with improved allowing drug‐testing. By adding other cell‐types TME, can be advanced heterotypic one.

Language: Английский

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Engineered Cell Microenvironments: A Benchmark Tool for Radiobiology

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

The development of engineered cell microenvironments for fundamental mechanobiology, in vitro disease modeling, and tissue engineering applications increased exponentially during the last two decades. In such context, radiobiology is a field research aiming at understanding effects ionizing radiation (e.g., X-rays/photons, high-speed electrons, protons) on biological (cancerous) tissues cells, particular terms DNA damage leading to death. Herein, perspective provides comparative assessment overview scaffold-free, scaffold-based, organ-on-a-chip models radiobiology, highlighting opportunities, limitations, future pathways improve currently existing approaches toward personalized cancer medicine.

Language: Английский

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Spheroid‐on‐a‐Chip Platforms for Tumor Microenvironment and Drug Development

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Despite significant advancements in oncology research and therapeutic interventions, cancer continues to be the leading cause of mortality worldwide. The key challenge addressing this pressing issue lies lack precision diagnosis a limited understanding nature how tumor microenvironment responds interventions. Research focusing on impact (TME) heterogeneity response drugs is crucial ensure efficient therapy. Conventional models exhibit constraints, including inability accurately imitate tumors’ complex 3D architecture dynamic microenvironment. Recent developments Spheroid‐On‐a‐Chip (SoC) technology provide alternative, offering closer that human tissue. This review explores recent SoC modeling, highlighting spheroid formation mechanisms techniques. applications platform mimic essential features their potential role anticancer drug development are summarized. advantages challenges technologies behind devices compared traditional vitro further discussed. Lastly, future directions for transforming improving suggested.

Language: Английский

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Unlocking the Potential Role of Decellularized Biological Scaffolds as a 3D Radiobiological Model for Low- and High-LET Irradiation

Cancers, Journal Year: 2024, Volume and Issue: 16(14), P. 2582 - 2582

Published: July 18, 2024

Introduction: Decellularized extracellular matrix (ECM) bioscaffolds have emerged as a promising three-dimensional (3D) model, but so far there are no data concerning their use in radiobiological studies. Material and Methods: We seeded two well-known radioresistant cell lines (HMV-II PANC-1) decellularized porcine liver-derived scaffolds irradiated them with both high- (Carbon Ions) low- (Photons) Linear Energy Transfer (LET) radiation order to test whether natural 3D-bioscaffold might be useful tool for research achieve an evaluation that could near possible what happens vivo. Results: Biological provided favorable 3D environment proliferation expansion. Cells did not show signs of dedifferentiation retained distinct phenotype coherently anatomopathological clinical behaviors. The response high LET was higher HMV-II PANC-1 compared the low LET. In particular, Carbon Ions reduced melanogenesis induced more cytopathic effects substantial deterioration photons. Conclusions: addition offering suitable model appropriate setting preclinical oncological analysis, we can attest seemed cost-effective due ease use, maintenance requirements, lack complex technology

Language: Английский

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A 3D Co-Culture Scaffold Approach to Assess Spatially Fractionated Radiotherapy Bystander and Abscopal Immune Effects on Clonogenic Survival

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4436 - 4436

Published: May 7, 2025

Spatially fractionated radiotherapy (SFRT) offers a promising approach for debulking large tumors by delivering high-dose radiation to fraction of the tumor volume. However, complex microenvironment necessitates models beyond traditional 2D cultures and resource-intensive animal studies SFRT investigations. Three-dimensional (3D) scaffold-based with an adequate cross-sectional area have emerged as uniquely suited platforms bridge this gap, providing more realistic platform GRID-based research. In study, we employed 3D co-culture scaffold model dissect contributions radiation-induced bystander effect, abscopal immune system response on clonogenic survival following GRID irradiation. MDA-MB-231 breast cancer cells were seeded commercial scaffolds irradiated at 20 Gy peak dose using lead grids three- six-hole patterns, exposing ~12.8% 25.7% area, respectively. An assessment reproductive cell revealed significant was notably lower than predicted based solely directly fraction. Evidence effect observed culturing non-irradiated in media exposed Furthermore, allogeneic peripheral blood mononuclear (PBMCs) modulated survival, additive when combined SFRT. These findings underscore presence indicate component, particularly three-hole configuration. This study established utility vitro effective elucidating SFRT-mediated biological responses.

Language: Английский

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Tumor Organoids: The Era of Personalized Medicine

Biochemistry (Moscow), Journal Year: 2024, Volume and Issue: 89(S1), P. S127 - S147

Published: Jan. 1, 2024

Language: Английский

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Tailoring epilepsy treatment: personalized micro-physiological systems illuminate individual drug responses

Annals of Medicine and Surgery, Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Introduction: Approximately 50 million people worldwide have epilepsy, with many not achieving seizure freedom. Organ-on-chip technology, which mimics organ-level physiology, could revolutionize drug development for epilepsy by replacing animal models in preclinical studies. Our goal is to determine if customized micro-physiological systems can lead tailored treatments epileptic patients. Materials and methods: A comprehensive literature search was conducted utilizing various databases, including PubMed, Ebscohost, Medline, the National Library of Medicine, using a predetermined strategy. We focused on articles that addressed role personalized individual responses discussed different types diagnosis, current treatment options. Additionally, explored components design considerations were reviewed identify challenges opportunities challenging cases. Results: The system offers more accurate cost-effective alternative traditional assessing effects, toxicities, disease mechanisms. Nevertheless, designing patient-specific presents critical considerations, integration analytical biosensors patient-derived cells, while addressing regulatory, material, biological complexities. Material selection, standardization, vascular systems, cost efficiency, real-time monitoring, ethical are also crucial successful use this technology development. Conclusion: future organ-on-chip holds great promise, potential integrate artificial intelligence machine learning

Language: Английский

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Investigating the Radiobiological Response to Peptide Receptor Radionuclide Therapy Using Patient-Derived Meningioma Spheroids

Cancers, Journal Year: 2024, Volume and Issue: 16(14), P. 2515 - 2515

Published: July 11, 2024

Peptide receptor radionuclide therapy (PRRT) using 177Lu-DOTA-TATE has recently been evaluated for the treatment of meningioma patients. However, current knowledge underlying radiation biology is limited, in part due to lack appropriate vitro models. Here, we demonstrate proof-of-concept a patient-derived 3D culture model assess short-term response therapies such as PRRT and external beam radiotherapy (EBRT). We established cultures (1 week) 16 meningiomas with high efficiency yield. In general, spheroids retained characteristics parental tumor during initial days culturing. For subset tumors, clear changes towards more aggressive phenotype were visible over time, indicating that method induced dedifferentiation cells. To efficacy, demonstrated specific uptake via somatostatin subtype 2 (SSTR2), which was highly overexpressed majority samples. DNA damage detectable an extended timeframe compared EBRT. Interestingly, levels after correlated SSTR2-expression tumors. Our can be used EBRT radiobiological studies. Further improvement this should pave way development relevant assessment long-term and, potentially, individual patient responses

Language: Английский

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