New Therapeutic Perspectives in Prostate Cancer: Patient-Derived Organoids and Patient-Derived Xenograft Models in Precision Medicine DOI Creative Commons
Vittoria Rago, Anna Perri, Silvia Di Agostino

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(10), P. 2743 - 2743

Published: Oct. 10, 2023

One of the major goals in advancement basic cancer research focuses on development new anticancer therapies. To understand molecular mechanisms progression, acquired drug resistance, and metastatic process, use preclinical vitro models that faithfully summarize properties tumor patients is still a necessity. The represented by diverse group cell clones, recent years, to reproduce models, monolayer cultures have been supplanted patient-derived xenograft (PDX) cultured organoids derived from patient (PDO). These proved indispensable for study microenvironment (TME) its interaction with cells. Prostate (PCa) most common neoplasia men world. It characterized genomic instability resistance conventional Despite advances diagnosis treatment, PCa remains leading cause death. Here, we review studies last 10 years as number papers growing very fast field. We also discuss discovered limitations challenges using organoid culture system PDXs studying prostate phenotype, performing testing, developing

Language: Английский

Current Advances in the Therapeutic Potential of Scutellarin: Novel Applications, Mechanisms, and Future Challenges. DOI Creative Commons
Great Iruoghene Edo, Alice Njolke Mafe, Patrick Othuke Akpoghelie

et al.

Phytomedicine Plus, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: Jan. 1, 2025

Language: Английский

Citations

2
Advanced Cellular Models for Rare Disease Study: Exploring Neural, Muscle and Skeletal Organoids DOI Open Access
Cristina Bombieri, Andrea Corsi, Elisabetta Trabetti

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1014 - 1014

Published: Jan. 13, 2024

Organoids are self-organized, three-dimensional structures derived from stem cells that can mimic the structure and physiology of human organs. Patient-specific induced pluripotent (iPSCs) 3D organoid model systems allow to be analyzed in a controlled environment simulate characteristics given disease by modeling underlying pathophysiology. The recent development cell models has offered scientific community an exceptionally valuable tool study rare diseases, overcoming limited availability biological samples limitations animal models. This review provides overview iPSC genetic engineering techniques used develop organoids. In particular, some applied neuronal, muscular skeletal diseases described. Furthermore, potential developing new therapeutic approaches discussed.

Language: Английский

Citations

9
3D Models Currently Proposed to Investigate Human Skin Aging and Explore Preventive and Reparative Approaches: A Descriptive Review DOI Creative Commons
Francesca Lombardi, Francesca Rosaria Augello, Alessia Ciafarone

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1066 - 1066

Published: Aug. 26, 2024

Skin aging is influenced by intrinsic and extrinsic factors that progressively impair skin functionality over time. Investigating the process requires thorough research using innovative technologies. This review explores use of in vitro human 3D culture models, serving as valuable alternatives to animal ones, research. The aim highlight benefits necessity improving methodology analyzing molecular mechanisms underlying aging. Traditional 2D including monolayers keratinocytes, fibroblasts, or melanocytes, even if providing cost-effective straightforward methods study critical processes such extracellular matrix degradation, pigmentation, effects secretome on cells, fail replicate complex tissue architecture with its intricated interactions. Advanced models (organoid cultures, “skin-on-chip” technologies, reconstructed skin, bioprinting) considerably enhance physiological relevance, enabling a more accurate representation peculiar features. By reporting advantages limitations this highlights importance advanced systems develop practical anti-aging preventive reparative approaches improve translational field. Further exploration these technologies will provide new opportunities for previously unexplored knowledge

Language: Английский

Citations

8
In vitro modelling of bacterial pneumonia: a comparative analysis of widely applied complex cell culture models DOI Creative Commons

Laure Mahieu,

Laurence Van Moll, Linda De Vooght

et al.

FEMS Microbiology Reviews, Journal Year: 2024, Volume and Issue: 48(2)

Published: Feb. 26, 2024

Abstract Bacterial pneumonia greatly contributes to the disease burden and mortality of lower respiratory tract infections among all age groups risk profiles. Therefore, laboratory modelling bacterial remains important for elucidating complex host–pathogen interactions determine drug efficacy toxicity. In vitro cell culture enables creation high-throughput, specific models in a tightly controlled environment. Advanced human specifically, can bridge research gap between classical two-dimensional animal models. This review provides an overview current status development cellular study infections, with focus on air–liquid interface models, spheroid, organoid, lung-on-a-chip For wide scale, comparative literature search, we selected six clinically highly relevant bacteria (Pseudomonas aeruginosa, Mycoplasma pneumoniae, Haemophilus influenzae, Mycobacterium tuberculosis, Streptococcus Staphylococcus aureus). We reviewed lines that are commonly used, as well trends discrepancies methodology, ranging from infection parameters assay read-outs. also highlighted importance model validation data transparency guiding field towards more

Language: Английский

Citations

7
Transforming Toxicity Assessment through Microphysiology, Bioprinting, and Computational Modeling DOI Open Access

Tamer A. Addissouky

Advances in Clinical Toxicology, Journal Year: 2024, Volume and Issue: 9(1), P. 1 - 14

Published: Jan. 1, 2024

Background: Traditional toxicity testing emphasizes animal models with growing concerns regarding predictive capacity, throughput and ethics. Rapid innovation surrounding human cell platforms, bioengineered tissues, omics techniques computational tools offers more modern alternatives aligned expanding knowledge of chemical biological pathways. These disruptive approaches promise immense potential to transform next-generation safety assessment drug development pipelines. Purpose: This review provides clinical researchers an updated, comprehensive perspective across evolving areas focus in new methods analysis latest advances translational context. Main Body: We survey progress two- three-dimensional cultures recapitulating tissue/organ complexity impossible conventional assays. Complementing this, modeling integrates structure-activity relationships, physicochemical properties physiological interactions predict pharmacokinetics silico. Expanding model organisms add further dimensionality demographic relevance. High-throughput imaging technologies unravel mechanisms illuminate biomarkers undetectable by standard measures. Specialized show high addressing toxicodynamic intricacies within disease contexts like diabetes NAFLD. Evaluating traditional medicines phytochemicals likewise represents area growth well-suited for contemporary platforms. Future outlook weighs remarkable advantages reducing demands, enabling precision toxicology links medicine overhauling core risk frameworks. Conclusion: intends catalyze discourse on strategic optimization priorities roadmaps towards fully unlocking the yet still emerging public health these poising transformation sciences centered human-focused models.

Language: Английский

Citations

6
Development of brain organoid technology derived from iPSC for the neurodegenerative disease modelling: a glance through DOI Creative Commons

Amirah Syamimi Jusop,

Kalaiselvaan Thanaskody,

Gee Jun Tye

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Aug. 3, 2023

Neurodegenerative diseases are adult-onset neurological conditions that notoriously difficult to model for drug discovery and development because most models unable accurately recapitulate pathology in disease-relevant cells, making it extremely explore the potential mechanisms underlying neurodegenerative diseases. Therefore, alternative of human or animal cells have been developed bridge gap allow impact new therapeutic strategies be anticipated more by trying mimic neuronal glial cell interactions many mechanisms. In tandem with emergence human-induced pluripotent stem which were first generated 2007, accessibility (hiPSC) derived from patients can differentiated into neurons, providing an unrivaled platform

Language: Английский

Citations

15
Standardization and consensus in the development and application of bone organoids DOI Creative Commons
Wang Jian, Xiao Chen, Ruiyang Li

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 15(2), P. 682 - 706

Published: Dec. 2, 2024

Organoids, self-organized structures derived from stem cells cultured in a specific three-dimensional (3D) vitro microenvironment, have emerged as innovative platforms that closely mimic vivo cellular behavior, tissue architecture, and organ function. Bone organoids, frontier organoid research, can replicate the complex functional characteristics of bone tissue. Recent advancements led to successful development including models callus, woven bone, cartilage, trabecular marrow. These organoids are widely utilized establishing bone-related disease models, injury repair, drug screening. However, significant discrepancies remain between current human skeletal tissues terms morphology functionality, limiting their ability accurately model physiology pathology. To address these challenges promote standardization construction, evaluation, application we convened experts research teams with substantial expertise field. By integrating existing findings, this consortium aims establish consensus guide future organoids.

Language: Английский

Citations

5
A Platform for Testing the Biocompatibility of Implants: Silicone Induces a Proinflammatory Response in a 3D Skin Equivalent DOI Creative Commons
Rima Nuwayhid, Torsten Schulz,

Frank Siemers

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 224 - 224

Published: Jan. 19, 2024

Biocompatibility testing of materials is carried out in 2D cell cultures or animal models despite serious limitations. 3D skin equivalents are advanced vitro for human skin. Silicone has been shown to be noncytotoxic but capable eliciting an immune response. Our aim was (1) establish a equivalent (2) assess the proinflammatory properties silicone. We developed coculture keratinocytes and fibroblasts resulting with implant using samples from breast implant. Samples without silicone were studied histologically immunohistochemically comparison native samples. Cytotoxicity assessed via LDH-assay, cytokine response ELISA. Histologically, our had four-layered epidermal dermal component. The presence tight junctions demonstrated immunofluorescence. only difference implants thinning. Implanting did not cause more death, however, inflammatory triggered. able organotypical implant, which can utilised studies on biocompatibility materials. This first integration into confirmed previous findings being non-cell-toxic exerting effect.

Language: Английский

Citations

4
Osteochondral organoids: current advances, applications, and upcoming challenges DOI Creative Commons

Maryam Faeed,

Mahsa Ghiasvand,

Bahar Fareghzadeh

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 21, 2024

Abstract In the realm of studying joint-related diseases, there is a continuous quest for more accurate and representative models. Recently, regenerative medicine tissue engineering have seen growing interest in utilizing organoids as powerful tools complex biological systems vitro. Organoids, three-dimensional structures replicating architecture function organs, provide unique platform investigating disease mechanisms, drug responses, regeneration. The surge organoid research fueled by need physiologically relevant models to bridge gap between traditional cell cultures vivo studies. Osteochondral emerged promising avenue this pursuit, offering better mimic intricate interactions within bone cartilage. This review explores significance osteochondral their development advancing our understanding treatment cartilage-related diseases. It summarizes organoids’ insights progress, focusing on composition, materials, sources, cultivation methods, well concept chips application scenarios. Additionally, we address limitations challenges these face, emphasizing necessity further overcome obstacles facilitate orthopedic

Language: Английский

Citations

4
Two- and Three-Dimensional In Vitro Models of Parkinson’s and Alzheimer’s Diseases: State-of-the-Art and Applications DOI Open Access
Cristina Solana‐Manrique, Ana María Sánchez‐Pérez, Nuria Paricio

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 620 - 620

Published: Jan. 13, 2025

In vitro models play a pivotal role in advancing our understanding of neurodegenerative diseases (NDs) such as Parkinson’s and Alzheimer’s disease (PD AD). Traditionally, 2D cell cultures have been instrumental elucidating the cellular mechanisms underlying these diseases. Cultured cells derived from patients or animal provide valuable insights into pathological processes at level. However, they often lack native tissue environment complexity, limiting their ability to fully recapitulate features. contrast, 3D offer more physiologically relevant platform by mimicking brain architecture. These can incorporate multiple types, including neurons, astrocytes, microglia, creating microenvironment that closely resembles brain’s complexity. Bioengineering approaches allow researchers better replicate cell–cell interactions, neuronal connectivity, disease-related phenotypes. Both advantages limitations. While simplicity scalability for high-throughput screening basic processes, enhanced physiological relevance Integrating findings both model systems NDs, ultimately aiding development novel therapeutic strategies. Here, we review existing study PD AD.

Language: Английский

Citations

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