Up-regulated mRNA expression of VEGFA receptors (FLT1 and KDR) in placentas after assisted reproductive technology fertilization DOI Creative Commons

A Mrozikiewicz,

Grażyna Kurzawińska, Michał Walczak

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 31, 2023

Abstract Objective Placental angiogenesis is a pivotal process for feto-maternal circulation and ensures efficient development of the placenta throughout pregnancy. Many factors during in vitro fertilization embryo transfer procedures may affect placental gene expression fetus development. The present study aimed to identify differences angiogenesis-related ( VEGFA , FGF2 FLT1 KDR ) profiles placentas after assisted reproductive technology natural conception healthy women. Methods In case-control study, term were collected from Caucasian women (N = 20) with uncomplicated pregnancy 9). mRNA was examined genes by real-time quantitative polymerase chain reaction (RT-qPCR). Group stratification performed comparison investigated between type transferred (fresh/frozen), place tissue donation (center/margin) newborns’ gender (male/female). Results ART significant down-regulation p 0.016) up-regulation 0.026) < 0.001) receptors observed. Genes encoding up-regulated both fresh (ET) frozen (FET) groups compared controls. For statistically difference observed group controls 0.032). Relative significantly higher ET FET 0.002). No different places gender. Conclusion We its pregnancies naturally conceived pregnancies. More research needed clarify these alterations that fetal health.

Language: Английский

Placental adaptations supporting fetal growth during normal and adverse gestational environments DOI Creative Commons
Amanda N. Sferruzzi‐Perri, Jorge López‐Tello, Esteban Salazar‐Petres

et al.

Experimental Physiology, Journal Year: 2022, Volume and Issue: 108(3), P. 371 - 397

Published: Dec. 9, 2022

Abstract The placenta is vital for mammalian development and a key determinant of life‐long health. It the interface between mother fetus responsible transporting nutrients oxygen needs to develop grow. Alterations in placental formation function, therefore, have consequences fetal growth birthweight, which turn determine perinatal survival risk non‐communicable diseases offspring later postnatal life. However, not static organ. As this review summarizes, research from multiple species has demonstrated that function alter developmentally substrates during normal gestation, as well when there greater competition polytocous monotocous with gestations. also adapts response gestational environment, integrating information about ability provide prevailing environment. In particular, structure (e.g. vascularity, surface area, blood flow, diffusion distance) transport capacity nutrient transporter levels activity) respond suboptimal environments, namely malnutrition, obesity, hypoxia maternal ageing. Mechanisms mediating environmentally induced homeostatic responses help support include imprinted genes, signalling pathways, subcellular constituents sexomes. Identification these strategies may inform therapies complicated human pregnancies advance understanding pathways underlying poor outcomes their health disease risk.

Language: Английский

Citations

38
Study protocol for an observational cohort study of heat stress impacts in pregnancy in The Gambia, West Africa DOI Creative Commons
Ana Bonell, Leonidas G. Ioannou, Abdul Karim Sesay

et al.

Wellcome Open Research, Journal Year: 2025, Volume and Issue: 9, P. 624 - 624

Published: Feb. 7, 2025

Climate change has resulted in an increase heat exposure globally. There is strong evidence that this increased stress associated with poor maternal and fetal outcomes, especially vulnerable populations. However, there remains understanding of the biological pathways mechanisms involved impact pregnancy. This observational cohort study 764 pregnant participants based sub-Saharan Africa, a geographical region at risk extreme events, aims to evaluate physiological biochemical changes occur pregnancy due stress. The key objectives are 1) map understand what environmental, social community factors exposure; 2) assess on health, e.g. strain, subjective psychological well-being, sleep activity level; 3) how impacts placenta structure function; 4) determine chronic birth outcomes; 5) explore epigenetic infant by per trimester. Pregnant women will be recruited from two distinct regions Gambia exploit naturally occurring gradient across country. Microclimate mapping area recruitment give detailed measurements. Participants asked wear watch-style device 28- 35-weeks gestational age heart rate, sleep. At end week, ultrasound scan performed size placental blood flow. delivery, outcomes recorded maternal, cord samples taken for epigenetic, histological evaluation. Evaluation neuro-behaviour final 1 month following birth.

Language: Английский

Citations

0
Up-regulated mRNA expression of VEGFA receptors (FLT1 and KDR) in placentas after assisted reproductive technology fertilization DOI Creative Commons

A Mrozikiewicz,

Grażyna Kurzawińska, Michał Walczak

et al.

Journal of Applied Genetics, Journal Year: 2024, Volume and Issue: 65(3), P. 531 - 540

Published: Jan. 18, 2024

Placental angiogenesis is a pivotal process for feto-maternal circulation and ensures efficient development of the placenta throughout pregnancy. Many factors during in vitro fertilization embryo transfer procedures may affect placental gene expression fetus development. The present study aimed to identify differences angiogenesis-related (VEGFA, FGF2, FLT1, KDR) profiles placentas after assisted reproductive technology natural conception healthy women. In case-control study, term were collected from Caucasian women (N = 20) with uncomplicated pregnancy 9). mRNA was examined VEGFA, KDR genes by real-time quantitative polymerase chain reaction (RT-qPCR). Group stratification performed comparison investigated between type transferred (fresh/frozen), place tissue donation (center/margin), newborns' gender (male/female). ART placentas, significant down-regulation VEGFA (p 0.016) up-regulation FLT1 0.026) < 0.001) receptors observed. Genes encoding up-regulated both fresh (ET) frozen (FET) groups compared controls. For gene, statistically difference observed group controls 0.032). Relative significantly higher ET FET 0.002). No different places gender. We its pregnancies naturally conceived pregnancies. More research needed clarify these alterations that fetal health.

Language: Английский

Citations

2
Infectious and environmental placental insults: from underlying biological pathways to diagnostics and treatments DOI
Samuel Chenge,

Harrison Ngure,

Bernard N. Kanoi

et al.

Pathogens and Disease, Journal Year: 2023, Volume and Issue: 81

Published: Jan. 1, 2023

Abstract Because the placenta is bathed in maternal blood, it exposed to infectious agents and chemicals that may be present mother’s circulation. Such exposures, which do not necessarily equate with transmission fetus, primarily cause placental injury, thereby impairing function. Recent research has improved our understanding of mechanisms by some are transmitted as well underlying their impact on fetal outcomes. However, less known about infection structure function, or infection-driven pathogenesis. Moreover, recent studies indicate noninfectious environmental accumulate placenta, but impacts function outcomes unknown. Critically, diagnosing insults during pregnancy very difficult currently, this possible only through postpartum examination. Here, emphasis humans, we discuss what chemical physiology particularly absence maternal–fetal transmission, highlight knowledge gaps potential implications for diagnosis intervention against pathologies.

Language: Английский

Citations

2
Placental Disorders as a Risk Factor for the Development of Postpartum Haemorrhage DOI Open Access
T. Е. Belokrinitskaya, A.G. Sidorkina, В.А. Мудров

et al.

Fundamental and Clinical Medicine, Journal Year: 2024, Volume and Issue: 9(2), P. 86 - 93

Published: June 21, 2024

The placenta is a functional link between mother and fetus during pregnancy the most important factor determining newborn infant health. Postpartum hemorrhage leading cause of maternal morbidity mortality worldwide affected by numerous factors including placental size. Here we analysed role disorders as risk for development postpartum hemorrhage. We screened available literature via PubMed, PubMed Central, Scopus, MEDLINE, ScienceDirect, Cochrane Library, eLibrary from 2001 to October 2023. Placental insufficiency may either hyperplasia (i.e., expansion meet nutritional needs growing fetus) or hypoplasia, which deficiency in nutrients oxygen slows down fetal growth development. Both these conditions significantly affect probability volume haemorrhage.

Language: Английский

Citations

0
Study protocol for an observational cohort study of heat stress impacts in pregnancy in The Gambia, West Africa DOI Creative Commons
Ana Bonell, Leonidas G. Ioannou, Abdul Karim Sesay

et al.

Wellcome Open Research, Journal Year: 2024, Volume and Issue: 9, P. 624 - 624

Published: Oct. 28, 2024

Climate change has resulted in an increase heat exposure globally. There is strong evidence that this increased stress associated with poor maternal and fetal outcomes, especially vulnerable populations. However, there remains understanding of the biological pathways mechanisms involved impact pregnancy. This observational cohort study 764 pregnant participants based sub-Saharan Africa, a geographical region at risk extreme events, aims to evaluate physiological biochemical changes occur pregnancy due stress. The key objectives are 1) map understand what environmental, social community factors exposure; 2) assess on health, e.g. strain, subjective psychological well-being, sleep activity level; 3) how impacts placenta structure function; 4) determine chronic birth outcomes; 5) explore epigenetic infant by per trimester. Pregnant women will be recruited from two distinct regions Gambia exploit naturally occurring gradient across country. Microclimate mapping area recruitment give detailed measurements. Participants asked wear watch-style device 28- 35-weeks gestational age heart rate, sleep. At end week, ultrasound scan performed size placental blood flow. delivery, outcomes recorded maternal, cord samples taken for epigenetic, histological evaluation. Evaluation neuro-behaviour final 1 month following birth.

Language: Английский

Citations

0
Up-regulated mRNA expression of VEGFA receptors (FLT1 and KDR) in placentas after assisted reproductive technology fertilization DOI Creative Commons

A Mrozikiewicz,

Grażyna Kurzawińska, Michał Walczak

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 31, 2023

Abstract Objective Placental angiogenesis is a pivotal process for feto-maternal circulation and ensures efficient development of the placenta throughout pregnancy. Many factors during in vitro fertilization embryo transfer procedures may affect placental gene expression fetus development. The present study aimed to identify differences angiogenesis-related ( VEGFA , FGF2 FLT1 KDR ) profiles placentas after assisted reproductive technology natural conception healthy women. Methods In case-control study, term were collected from Caucasian women (N = 20) with uncomplicated pregnancy 9). mRNA was examined genes by real-time quantitative polymerase chain reaction (RT-qPCR). Group stratification performed comparison investigated between type transferred (fresh/frozen), place tissue donation (center/margin) newborns’ gender (male/female). Results ART significant down-regulation p 0.016) up-regulation 0.026) < 0.001) receptors observed. Genes encoding up-regulated both fresh (ET) frozen (FET) groups compared controls. For statistically difference observed group controls 0.032). Relative significantly higher ET FET 0.002). No different places gender. Conclusion We its pregnancies naturally conceived pregnancies. More research needed clarify these alterations that fetal health.

Language: Английский

Citations

0