Thalamic Neuron Resilience during Osmotic Demyelination Syndrome (ODS) Is Revealed by Primary Cilium Outgrowth and ADP-ribosylation factor-like protein 13B Labeling in Axon Initial Segment DOI Open Access
Jacques Gilloteaux,

Kathleen De Swert,

Valérie Suain

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16448 - 16448

Published: Nov. 17, 2023

A murine osmotic demyelinating syndrome (ODS) model was developed through chronic hyponatremia, induced by desmopressin subcutaneous implants, followed precipitous sodium restoration. The thalamic ventral posterolateral (VPL) and posteromedial (VPM) relay nuclei were the most demyelinated regions where neuroglial damage could be evidenced without immune response. This report showed that following 12 h 48 time lapses after rebalancing osmolarity, amid ODS-degraded outskirts, some resilient neuronal cell bodies built up primary cilium axon hillock extended into initial segments (AIS) ADP-ribosylation factor-like protein 13B (ARL13B)-immunolabeled rod-like shape content revealed. These AIS-labeled shaft lengths appeared proportional with distance of away from ODS damaged epicenter correction hyponatremia. Fine structure examination verified these neuron abundant transcriptions translation marked ARL13B labeling associated neurotubules their complex cytoskeletal macromolecular architecture. necessitated energetic transport to organize restore those AIS core zone in model. labeled structures substantiate how resilience occurred as possible steps a healing course out ODS.

Language: Английский

The dynamic axon initial segment: From neuronal polarity to network homeostasis DOI Creative Commons
Amélie Fréal, Casper C. Hoogenraad

Neuron, Journal Year: 2025, Volume and Issue: 113(5), P. 649 - 669

Published: Feb. 12, 2025

The axon initial segment (AIS) is a highly specialized compartment in neurons that resides between axonal and somatodendritic domains. localization of the AIS proximal part essential for its two major functions: generating modulating action potentials maintaining neuron polarity. Recent findings revealed incredibly stable generated from dynamic components can undergo extensive structural functional changes response to alterations activity levels. These activity-dependent structure function have profound consequences neuronal functioning, plasticity has emerged as key regulator network homeostasis. This review highlights functions AIS, architecture, how organization remodeling are influenced by developmental both acute chronic adaptations. It also discusses mechanisms underlying these processes explores dysregulated may contribute brain disorders.

Language: Английский

Citations

0
Analgesic and Anti-inflammatory Properties of Dendrobium nobile DOI

Surendra Sarsaiya,

Archana Jain, Qihai Gong

et al.

CABI eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 118 - 142

Published: March 24, 2025

Language: Английский

Citations

0
Identification of candidate genes involved in Zika virus-induced reversible paralysis of mice DOI Creative Commons
John D. Morrey,

Venkatraman Siddharthan,

Wang Hong

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 23, 2025

Language: Английский

Citations

0
The Inflammation/NF-κB and BDNF/TrkB/CREB Pathways in the Cerebellum Are Implicated in the Changes in Spatial Working Memory After Both Morphine Dependence and Withdrawal in Rat DOI
Shamseddin Ahmadi, Mohammad Majidi,

Maryam Koraei

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(9), P. 6721 - 6733

Published: Feb. 12, 2024

Language: Английский

Citations

3
Actin polymerization and longitudinal actin fibers in axon initial segment plasticity DOI Creative Commons
David Micinski, Pirta Hotulainen

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: May 10, 2024

The location of the axon initial segment (AIS) at junction between soma and neurons makes it instrumental in maintaining neural polarity as site for action potential generation. AIS is also capable large-scale relocation an activity-dependent manner. This represents a form homeostatic plasticity which regulate their own excitability by changing size and/or position AIS. While important proper functionality AIS-containing neurons, cellular molecular mechanisms are poorly understood. Here, we analyzed changes actin cytoskeleton during using 3D structured illumination microscopy (3D-SIM). We showed that number longitudinal fibers increased transiently 3 h after induction. further polymerization, especially formin mediated required formation fibers. From family proteins, Daam1 localized to ends These results indicate active re-organization plasticity.

Language: Английский

Citations

2
Multiomics Reveals Biological Mechanisms Linking Macroscale Structural Covariance Network Dysfunction With Neuropsychiatric Symptoms Across the Alzheimer’s Disease Continuum DOI Creative Commons
Jiwei Jiang, Kun Zhao, Wenyi Li

et al.

Biological Psychiatry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

1
Identification of a novel KCNT2 variant in a family with developmental and epileptic encephalopathies: a case report and literature review DOI Creative Commons

F Cui,

Tuoya Wulan,

Qian Zhang

et al.

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: March 6, 2024

Background: Developmental and epileptic encephalopathies (DEEs) are a group of heterogeneous neurodevelopmental diseases characterized mainly by developmental delay/intellectual disability early-onset epilepsy. Researchers have identified variations in the KCNT2 gene (OMIM* 610044) as cause DEE type 57 (MIM# 617771). Case presentation: We report this study 46-year-old woman who presented with epilepsy, intellectual disability, hypertrichosis, coarse facial features, short stature. Besides, there were four other affected individuals her family history, including two elder brothers, younger brother, their mother. collected blood samples from proband, clinically normal daughter for genetic analysis. Clinical exome sequencing revealed novel heterozygous variant (NM_198503: c.188G>A, p.Arg63His) proband while did not carry variant. Furthermore, we reviewed all 25 patients literature variants compared phenotypes. Conclusion: Epilepsy disability/developmental delay occur almost variants. -relevant DEEs partially overlap clinical phenotypes K ATP channel diseases, particularly hypertrichosis distinctive features.

Language: Английский

Citations

0
Variability of axon initial segment geometry and its impact on hippocampal pyramidal cell function DOI Creative Commons
Nikolas A Stevens,

Maximilian Achilles,

Juri Monath

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

ABSTRACT Action potentials, the primary information units of nervous system, are usually generated at axon initial segment (AIS). Changes in length and position AIS associated with alterations neuronal excitability but there is only limited about baseline structural variability AIS. This work provides a comprehensive atlas diversity proximal cell geometries across all anatomical axes murine hippocampus, encompassing dorsal-ventral, superficial-deep, proximal-distal regions. We analyzed morphology 3,936 hippocampal pyramidal neurons 12 animals both sexes, focusing on length, position, their association cellular features such as soma dendritic geometries. Notably, axon-carrying dendrites were significantly more common ventral compared to dorsal areas, suggesting functional adaptation regional demands. Validation this finding human samples confirms translational relevance our model. employed NEURON simulations assess implications variability. Here, variation geometry minimally contributed homeostasis, instead increased heterogeneity response patterns neurons.

Language: Английский

Citations

0
Thalamic Neuron Resilience during Osmotic Demyelination Syndrome (ODS) Is Revealed by Primary Cilium Outgrowth and ADP-ribosylation factor-like protein 13B Labeling in Axon Initial Segment DOI Open Access
Jacques Gilloteaux,

Kathleen De Swert,

Valérie Suain

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(22), P. 16448 - 16448

Published: Nov. 17, 2023

A murine osmotic demyelinating syndrome (ODS) model was developed through chronic hyponatremia, induced by desmopressin subcutaneous implants, followed precipitous sodium restoration. The thalamic ventral posterolateral (VPL) and posteromedial (VPM) relay nuclei were the most demyelinated regions where neuroglial damage could be evidenced without immune response. This report showed that following 12 h 48 time lapses after rebalancing osmolarity, amid ODS-degraded outskirts, some resilient neuronal cell bodies built up primary cilium axon hillock extended into initial segments (AIS) ADP-ribosylation factor-like protein 13B (ARL13B)-immunolabeled rod-like shape content revealed. These AIS-labeled shaft lengths appeared proportional with distance of away from ODS damaged epicenter correction hyponatremia. Fine structure examination verified these neuron abundant transcriptions translation marked ARL13B labeling associated neurotubules their complex cytoskeletal macromolecular architecture. necessitated energetic transport to organize restore those AIS core zone in model. labeled structures substantiate how resilience occurred as possible steps a healing course out ODS.

Language: Английский

Citations

1