Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 171, P. 68 - 84
Published: Sept. 19, 2023
Language: Английский
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 2241 - 2264
Published: March 1, 2024
Abstract: Recently, gene therapy has become a subject of considerable research and been widely evaluated in various disease models. Though it is considered as stand-alone agent for COVID-19 vaccination, still suffering from the following drawbacks during its translation bench to bedside: high sensitivity exogenous nucleic acids enzymatic degradation; severe side effects induced either by or components formulation; difficulty cross barriers before reaching therapeutic target. Therefore, successful application therapy, safe reliable transport vector urgently needed. Extracellular vesicles (EVs) are ideal candidate delivery drugs owing their low immunogenicity, good biocompatibility toxicity. To better understand properties EVs advantages drug vehicles, this review covers origin methods generation, well common isolation purification research, with pros cons discussed. Meanwhile, engineering also highlighted. In addition, paper presents progress EVs-mediated microRNAs, small interfering RNAs, messenger plasmids, antisense oligonucleotides. We believe will provide theoretical basis development drugs. Keywords: extracellular vesicles, system,
Language: Английский
Citations
14
ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(4), P. 2128 - 2139
Published: April 3, 2024
Cancer research has made significant progress in recent years, and extracellular vesicles (EVs) based cancer investigation reveals several facts about cancer. Exosomes are a subpopulation of EVs. In the present decade, exosomes is mostly highlighted for theranostic research. Tumor cell derived (TEXs) promote but there multiple sources that can be used as therapeutic agents (plant exosomes, stem cell-derived modified or synthetic exosomes). Stem cells regenerative medicine faces numerous challenges, such tumor development, cellular reprogramming etc., therefore addressing these complications becomes essential. serves an answer to problems offers better solution. Global indicates also play dual role system by either inhibiting promoting Modified which genetically engineered surface increase efficacy properties considered target above concerns. However, difficulties associated with include variations heterogenity, isolation protocols, large scale production, have managed effectively. this review, we explore biogenesis, exosome sources, drug delivery, clinical trial related challenges domain future orientation. This article may encourage researchers develop effective affordable
Language: Английский
Citations
11
Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 226, P. 116399 - 116399
Published: June 27, 2024
Language: Английский
Citations
9
Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 27 - 27
Published: Jan. 1, 2025
Exosomes are small extracellular vesicles and crucial in intercellular communication. Interestingly, tumor-derived exosomes carry oncogenic molecules, such as proteins microRNAs, which can reprogram recipient cells, promote angiogenesis, stimulate cancer pre-metastatic niche, supporting growth metastasis. On the other hand, their biocompatibility, stability, ability to cross biological barriers make them attractive candidates for drug delivery. Recent advances have shown potential be used early disease detection targeted therapy by delivering therapeutic agents specifically tumor sites. Despite promising applications, a number of challenges remain, including exosome isolation characterization, well inherent heterogeneity. Thus, current review aims describe roles health disease, discuss that hinder development into becoming useful medical tools.
Language: Английский
Citations
1
Neuroglia, Journal Year: 2025, Volume and Issue: 6(1), P. 3 - 3
Published: Jan. 4, 2025
Background/Objectives: Glioblastoma (GBM), a highly aggressive grade IV astrocytoma, poses major therapeutic challenge due to the resistance of cancer stem cells (CSCs) existing within its cell population conventional therapies. Recently, we reported that RNA interference targeting CSC protection mechanism significantly improved efficacy. However, challenges remain, including limited transfection efficiency in neural and difficulty crossing blood–brain barrier (BBB). Methods: In this study, investigated potential exosome-mediated delivery cargo GBM by engineering exosomes carry green fluorescent protein (GFP) expressing brain-homing peptide (BHP) on their surface, which has high affinity cells. Results: We found BHP-modified doubled GFP efficacy from 20% 40%, outperforming traditional methods like lipofection vitro. vivo, demonstrated an ability cross BBB targeted brain regions following intranasal subcutaneous administration. Conclusions: These results underscore engineered for efficient enhance against tumors suggest novel avenues delivering biomolecules treatment neurological disorders.
Language: Английский
Citations
1
Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14
Published: Jan. 16, 2025
Oral squamous cell carcinoma (OSCC) accounts for approximately 90% of all oral cancers, significantly impacting the survival and quality life patients. Exosomes, small extracellular vesicles released by cells, play a crucial role in intercellular communication cancer. Nevertheless, their function mechanism OSCC remain elusive. Search Pubmed, Web Science, Cochrane Library using keywords OSCC, exome, diagnosis, treatment to review research progress exome OSCC. Based on these results, this starting from biosynthesis, structure, contents exosomes, elaborates exosomes diagnosis It explores potential briefly describes challenges researchers currently face.
Language: Английский
Citations
1
Small, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 3, 2025
Abstract The advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐based genome editing technologies has opened up groundbreaking possibilities for treating a wide spectrum genetic disorders and diseases. However, the success these relies heavily on development efficient safe delivery systems. Among most promising approaches are natural synthetic nanocarrier‐mediated systems, including viral vectors, extracellular vesicles (EVs), engineered cellular membrane particles, liposomes, various nanoparticles. These carriers enhance efficacy CRISPR system by providing unique combination efficiency, specificity, reduced immunogenicity. Synthetic such as liposomes nanoparticles facilitate with high reproducibility customizable functions. Viral renowned their transduction efficiency broad tropism, serve powerful vehicles delivering components to cell types. EVs, RNA proteins, offer stealth mechanism evade immune detection, allowing targeted editors minimal off‐target effects. Engineered particles further improve simulating environment, enhancing uptake, minimizing response. This review explores innovative integration nanocarrier focusing recent advancements, applications, future directions in therapeutic editing.
Language: Английский
Citations
1
Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: 106, P. 106696 - 106696
Published: Feb. 6, 2025
Language: Английский
Citations
1
World Journal of Gastrointestinal Oncology, Journal Year: 2025, Volume and Issue: 17(3)
Published: Feb. 13, 2025
In this article, we revisit an which specifically focuses on the utilization of exosomes derived from human bone marrow mesenchymal stem cells (MSCs) for targeted delivery gemcitabine in pancreatic cancer treatment. The experimental results demonstrated that exosome-based drug system MSCs significantly augmented apoptosis cells. biocompatibility, targeting specificity, and low immunogenicity render them as optimal carriers delivery, enabling precise administration therapeutics to diseased tissues while mitigating adverse effects, thereby achieving treatment enhancing anti-tumor efficacy. However, clinical application exosome platforms oncology still presents challenges, necessitating further optimization ensure their stability This study elucidating advantages a platform, exploring MSC-derived therapy, discussing potential future directions
Language: Английский
Citations
1
Burns & Trauma, Journal Year: 2025, Volume and Issue: 13
Published: Jan. 1, 2025
Poor wound healing is a refractory process that places an enormous medical and financial burden on diabetic patients. Exosomes have recently been recognized as crucial players in the of lesions. They excellent stability, homing effects, biocompatibility, reduced immunogenicity novel cell-free therapies. In addition to transporting cargos target cells enhance intercellular communication, exosomes are beneficial nearly every phase healing. participate modulating inflammatory response, accelerating proliferation reepithelization, increasing angiogenesis, regulating extracellular matrix remodeling. Accumulating evidence indicates hydrogels or dressings conjunction with can prolong duration exosome residency wounds. This review provides overview mechanisms, delivery, clinical application, engineering, existing challenges use repair. We also propose future directions for biomaterials incorporating exosomes: 2D 3D scaffolds, loaded healing-promoting gases, intelligent biomaterials, prospect systematic application exosomes. These findings may might shed light treatments enlighten some studies improve quality life among diabetes
Language: Английский
Citations
1