Functional Classification and Interaction Selectivity Landscape of the Human SH3 Domain Superfamily DOI Open Access

Neda S. Jasemi Kazemein,

Mehrnaz Mehrabipour,

Eva Magdalena Estirado

et al.

Published: Dec. 14, 2023

SRC homology 3 (SH3) domains are critical interaction modules that orchestrate the assembly of protein complexes involved in diverse biological processes. They facilitate transient protein-protein interactions by selectively interacting with proline-rich motifs (PRMs). A database search revealed 298 SH3 221 human proteins. Multiple sequence alignment is useful for phylogenetic analysis and determination their selectivity towards PRM-containing peptides (PRPs). However, a more precise functional classification achieved constructing tree only from PRM-binding residues using existing SH3-PRP structures biochemical data to determine specificity within each 10 families particular PRPs. In addition, C-terminal domain RAS activator SOS1 covers 13 14 recognized consensus motifs, encompassing differential PRP pattern among all families. To evaluate binding capabilities affinities, we conducted fluorescence dot blot polarization experiments 25 representative various PRPs derived SOS1. Our has identified 45 pairs affinities ranging 0.2 125 micromolar out 300 tested potential new interactors Furthermore, it establishes framework bridge gap between provides predictive insights into PRMs based on specifications. This novel enhance understanding networks mediated SH3-PRM be utilized as general approach other domain-peptide interactions.

Language: Английский

CD133-Dependent Activation of Phosphoinositide 3-Kinase /AKT/Mammalian Target of Rapamycin Signaling in Melanoma Progression and Drug Resistance DOI Creative Commons
Naji Kharouf,

Thomas W. Flanagan,

Abdulhadi A. AlAmodi

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(3), P. 240 - 240

Published: Jan. 26, 2024

Melanoma frequently harbors genetic alterations in key molecules leading to the aberrant activation of PI3K and its downstream pathways. Although role PI3K/AKT/mTOR melanoma progression drug resistance is well documented, targeting pathway showed less efficiency clinical trials than might have been expected, since suppression PI3K/mTOR signaling pathway-induced feedback loops mostly associated with compensatory pathways such as MAPK/MEK/ERK. Consequently, development intrinsic acquired can occur. As a solid tumor, notorious for heterogeneity. This be expressed form genetically divergent subpopulations including small fraction cancer stem-like cells (CSCs) non-cancer stem (non-CSCs) that make most tumor mass. Like other CSCs, (MSCs) are characterized by their unique cell surface proteins/stemness markers In addition function robust marker stemness properties, CD133 crucial maintenance properties resistance. Herein, CD133-dependent regulation progression, resistance, recurrence reviewed.

Language: Английский

Citations

4
Functional heterogeneity of mesenchymal stem cells and their therapeutic potential in the K18-hACE2 mouse model of SARS-CoV-2 infection DOI Creative Commons

Kátia Nunes da Silva,

Fernanda Martins Marim,

Gisele Vieira Rocha

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 23, 2025

Language: Английский

Citations

0
Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish DOI

Guangjing Zhang,

Jing Huang, Liangyue Peng

et al.

Reproduction and Breeding, Journal Year: 2025, Volume and Issue: 5(2), P. 21 - 26

Published: March 12, 2025

Language: Английский

Citations

0
VHI-Pred: A Multi-Feature-Based Tool for Predicting Human–Virus Protein–Protein Interactions DOI

Rasool Sahragard,

Masoud Arabfard, Ali Ahmadi

et al.

Molecular Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 5, 2025

Language: Английский

Citations

0
Unveiling an unexpected redox regulation of the folding, function and inhibition in the phosphotyrosine binding domain of FRS2 DOI Creative Commons
Valeria Pennacchietti, Livia Pagano,

Mariana Di Felice

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142478 - 142478

Published: April 1, 2025

Language: Английский

Citations

0
The Ubiquitin-Associated and SH3 Domain-Containing Proteins (UBASH3) Family in Mammalian Development and Immune Response DOI Open Access
Katarina Vukojević,

Violeta Šoljić,

Vlatka Martinović

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1932 - 1932

Published: Feb. 5, 2024

UBASH3A and UBASH3B are protein families of atypical tyrosine phosphatases that function as regulators various cellular processes during mammalian development. As has only mild phosphatase activity, its regulatory effects based on the phosphatase-independent mechanisms. On contrary, strong suppression receptor signalling is mediated by Syk Zap-70 kinases. The functions particularly evident in lymphoid tissues kidney These also known to play key roles autoimmunity neoplasms. However, their involvement development largely unknown discussed this review.

Language: Английский

Citations

3
Functional Classification and Interaction Selectivity Landscape of the Human SH3 Domain Superfamily DOI Creative Commons
Neda S. Kazemein Jasemi, Mehrnaz Mehrabipour,

Eva Magdalena Estirado

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(2), P. 195 - 195

Published: Jan. 20, 2024

SRC homology 3 (SH3) domains are critical interaction modules that orchestrate the assembly of protein complexes involved in diverse biological processes. They facilitate transient protein–protein interactions by selectively interacting with proline-rich motifs (PRMs). A database search revealed 298 SH3 221 human proteins. Multiple sequence alignment is useful for phylogenetic analysis and determination their selectivity towards PRM-containing peptides (PRPs). However, a more precise functional classification achieved constructing tree only from PRM-binding residues using existing domain–PRP structures biochemical data to determine specificity within each 10 families particular PRPs. In addition, C-terminal domain RAS activator SOS1 covers 13 14 recognized consensus motifs, encompassing differential PRP pattern among all families. To evaluate binding capabilities affinities, we conducted fluorescence dot blot polarization experiments 25 representative various PRPs derived SOS1. Our has identified 45 pairs, affinities ranging 0.2 125 micromolar, out 300 tested potential new domain-SOS1 interactions. Furthermore, it establishes framework bridge gap between provides predictive insights into PRMs based on specifications. This novel enhance understanding networks mediated domain–PRM be utilized as general approach other domain–peptide

Language: Английский

Citations

2
Motor proteins, spermatogenesis and testis function DOI
Lingling Wang,

Tiao Bu,

Xiaolong Wu

et al.

Advances in protein chemistry and structural biology, Journal Year: 2024, Volume and Issue: unknown, P. 381 - 445

Published: Jan. 1, 2024

Language: Английский

Citations

1
The Sdp-SH3b2 domain contained in Lactobacillus johnsonii N6.2-derived extracellular vesicles inhibit murine norovirus replication DOI Creative Commons
Danilo R. Silva,

Asra B. Sharjeel,

Reagan Beliakoff

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 5, 2024

The internalization of Lactobacillus johnsonii N6.2 extracellular vesicles (EVs) by cells results in a significant induction the 2’,5’-oligoadenylate synthetase (OAS) pathway. It also induces expression IFI44L, MX1, MX2 and DDX60 . In this work, we evaluated whether antiviral response induced L. N6.2-derived EVs, has an inhibitory effect on RNA viral insult using murine norovirus (MNV-1) as infection model. We found that RAW 264.7 Macrophages treated with EVs significantly decreased levels MNV-1 genome. These were consistent increase Oas1b, Oas2, Oasl, Mx1, Mx2 Ifi44l (6 hours post infection). Out six proteins enriched SH3b2 domain Sdp was only protein effector molecule able to recapitulate activation OAS C57BL6 mice, administration live N6.2, Sdp-SH3b2/liposomes titers distal ileum, contrast controls PBS liposomes alone did not affect MNV-1. establish Sdp, which is derived can orchestrate control infections vivo

Language: Английский

Citations

1
Progesterone receptor-Grb2 interaction is associated with better outcomes in breast cancer DOI

Nattamolphan Wittayavimol,

Erina Iwabuchi, Prangwan Pateetin

et al.

The Journal of Steroid Biochemistry and Molecular Biology, Journal Year: 2023, Volume and Issue: 237, P. 106441 - 106441

Published: Dec. 8, 2023

Language: Английский

Citations

2