Mesenchymal Stem Cell-Derived Exosomes as Drug Delivery Vehicles in Disease Therapy

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7715 - 7715

Published: July 14, 2024

Exosomes are small vesicles containing proteins, nucleic acids, and biological lipids, which responsible for intercellular communication. Studies have shown that exosomes can be utilized as effective drug delivery vehicles to accurately deliver therapeutic substances target tissues, enhancing effects reducing side effects. Mesenchymal stem cells (MSCs) a class of widely used tissue engineering, regenerative medicine, immunotherapy. derived from MSCs special immunomodulatory functions, low immunogenicity, the ability penetrate tumor high yield, expected engineered into efficient systems. Despite promising promise MSC-derived exosomes, exploring their optimal preparation methods, drug-loading modalities, potential remains challenging. Therefore, this article reviews related characteristics, application, risks systems in order find breakthroughs.

Language: Английский

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Methods, Mechanisms, and Application Prospects for Enhancing Extracellular Vesicle Uptake

Current Medical Science, Journal Year: 2024, Volume and Issue: 44(2), P. 247 - 260

Published: April 1, 2024

Language: Английский

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The potential of exosomes in regenerative medicine and in the diagnosis and therapies of neurodegenerative diseases and cancer

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: March 13, 2025

Exosomes, nanosized extracellular vesicles released by various cell types, are intensively studied for the diagnosis and treatment of cancer neurodegenerative diseases, they also display high usability in regenerative medicine. Emphasizing their diagnostic potential, exosomes serve as carriers disease-specific biomarkers, enabling non-invasive early detection personalized The cargo loading with therapeutic agents presents an innovative strategy targeted drug delivery, minimizing off-target effects optimizing interventions. In medicine, play a crucial role intercellular communication, facilitating tissue regeneration through transmission bioactive molecules. While acknowledging existing challenges standardization scalability, ongoing research efforts aim to refine methodologies address regulatory considerations. summary, this review underscores transformative potential reshaping landscape medical interventions, particular emphasis on cancer,

Language: Английский

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Engineered ATP-Loaded Extracellular Vesicles Derived from Mesenchymal Stromal Cells: A Novel Strategy to Counteract Cell ATP Depletion in an In Vitro Model

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3424 - 3424

Published: April 5, 2025

The use of adenosine triphosphate (ATP) has shown promising effects in alleviating ischemic damage across various tissues. However, the penetration ATP into kidney tubular cells presents a challenge due to their unique anatomical and physiological properties. In this study, we introduce novel bioinspired drug delivery system utilizing extracellular vesicles (EVs) derived from mesenchymal stromal (MSCs) engineered carry ATP. ATP-loaded liposomes (ATP-LPs) EVs (ATP-EVs) were prepared using microfluidic technology, followed by characterization morphology (DLS, NTA, SEM, TEM), content, release rate at 37 °C (pH 7.4). Additionally, efficacy ATP-LPs ATP-EVs was evaluated vitro on renal (HK2 cells) under chemically induced ischemia. results indicated successful enrichment EVs, with showing no significant changes or size compared naïve EVs. Notably, demonstrated superior retention ATP-LPs, protecting degradation environment. an ATP-depleted HK2 cell model, only effectively restored levels, preserving viability reducing apoptotic gene expression (BCL2-BAX). This study is first successfully demonstrate direct as carriers.

Language: Английский

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Exosomes derived from human amniotic mesenchymal stem cells promotes angiogenesis in hUVECs by delivering novel miRNA N-194

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: May 6, 2025

Abstract Background To investigate the effect and mechanism of exosomes derived from human amniotic mesenchymal stem cells (hAMSC-Exos) promoting angiogenesis. Methods HAMSC-Exos were isolated using ultracentrifugation characterized by transmission electron microscopy, NTA, Western blot. The uptake hAMSC-Exos hUVECs was analyzed PKH-26 labeling, on angiogenesis in umbilical vein endothelial cell viability assay, Transwell migration Matrigel tube formation plug assays nude mice. Bioinformatics methods used to analyze miRNA high-throughput sequencing data hAMSC-Exos, RT-qPCR validate novel miRNAs. with high low N-194 expression obtained transfection, respectively. Target genes predicted TargetScan, mRNA protein levels potential target blot after mimics transfection. Interaction between miRNAs detected dual-luciferase reporter assay. overexpressed roles influence function determined analyzing Results extracted showed saucer-shaped under NTA results particle size 115.6 ± 38.6 nm. positive CD9, CD63, CD81 verified treatment significantly increased proliferation, migration, contained N-194, N-314, N-19, N-393, N-481, higher. Exos hAMSCs which transfected FAM-N-194 able deliver hUVECs. promoted transfection reduced gene ING5, luciferase activities expressed wild-type vectors for ING5. ING5 overexpression angiogenic capacity suppressed HSP27 PLCG2. Conclusions promotes delivering targets Graphical

Language: Английский

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Exploring the Therapeutic Potential of Extracellular Vesicles Derived from Human Immature Dental Pulp Cells on Papillary Thyroid Cancer

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8178 - 8178

Published: July 26, 2024

Mesenchymal stem-cell-derived extracellular vesicles (MSC-EVs) have been increasingly investigated for cancer therapy and drug delivery, they offer an advanced cell-free therapeutic option. However, their overall effects efficacy depend on various factors, including the MSC source cargo content. In this study, we isolated EVs from conditioned medium of human immature dental pulp stem cells (hIDPSC-EVs) two papillary thyroid (PTC) cell lines (BCPAP TPC1). We observed efficient uptake hIDPSC-EVs by both PTC lines, with a notable impact gene regulation, particularly in Wnt signaling pathway BCPAP cells. no significant proliferation were observed. Conversely, significantly reduced invasive capacity after 120 h treatment. These vitro findings suggest potential management emphasize need further research to develop novel effective treatment strategies. Furthermore, successful internalization underscores use as nanocarriers anti-cancer agents.

Language: Английский

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Application of extracellular vesicles in diabetic osteoporosis

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 10, 2024

As the population ages, occurrence of osteoporosis is becoming more common. Diabetes mellitus one factors in development osteoporosis. Compared with general population, incidence significantly higher diabetic patients. Diabetic (DOP) a metabolic bone disease characterized by abnormal tissue structure due to hyperglycemia and insulin resistance, reduced strength increased risk fractures. This complex mechanism that occurs at cellular level such as blood vessels, inflammation, resistance. Although application some drugs clinical practice can reduce DOP, fractures caused DOP still very high. Extracellular vesicles (EVs) are new communication mode between cells, which transfer miRNAs proteins from mother cells target through membrane fusion, thereby regulating function cells. In recent years, role EVs pathogenesis has been widely demonstrated. this article, we first describe changes microenvironment Second, DOP. Finally, summarize research progress challenges

Language: Английский

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