Biomedicine, Journal Year: 2024, Volume and Issue: 14(4)
Published: Dec. 1, 2024
Introduction: Although the HaCaT keratinocyte model has been used in previous research to study effects of anti-psoriatic agents, there is still a lack comprehensive understanding mechanism imiquimod (IMQ)-induced proliferation and signal transduction psoriasis-like keratinocytes.
Language: Английский
Nano Materials Science, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
1
Immunobiology, Journal Year: 2024, Volume and Issue: 229(5), P. 152840 - 152840
Published: Aug. 3, 2024
Due to the urgent need create appropriate treatment techniques, which are currently unavailable, LPS-induced sepsis has become a serious concern on global scale. The primary active component in pathophysiology of inflammatory diseases such as is Gram-negative bacterial lipopolysaccharide (LPS). LPS interacts with cell surface TLR4 macrophages, causing formation reactive oxygen species (ROS), TNF-α, IL-1β and oxidative stress. It also significantly activates MAPKs NF-κB pathway. Excessive production pro-inflammatory cytokines one characteristic features onset progression inflammation. Cytokines mainly signal through JAK/STAT We hypothesize that blocking along TNFR1 might be beneficial suppressing effects STAT1/STAT3 due stimulation SOCS3 proteins. Prior challenge, macrophages were treated antibodies against either individually or combination. On analysis macrophage populations by flowcytometry, it was seen receptor blockade facilitated phenotypic shift M1 towards M2 resulting lowered Blocking TLR4/TNFR1 upregulated mTOR expressions enabled transition anti-inflammatory phenotype, crucial curbing responses. Also reduction IL-6, activation STAT1 STAT3 molecules observed our combination group. All these results indicated neutralization both provide new insights establishing an alternative therapeutic strategy for LPS-sepsis.
Language: Английский
Citations
6
Biomolecules, Journal Year: 2024, Volume and Issue: 14(7), P. 763 - 763
Published: June 26, 2024
Atopic dermatitis, psoriasis and lichen sclerosus are among the most challenging conditions treated by dermatologists worldwide, with potentially significant physical, social psychological impacts. Emerging evidence suggests that autologous-platelet-rich plasma could be used to manage skin inflammation. However, presence of soluble autoimmune components hinder their therapeutic potential. The aim this study was analyze proteomic profile rich in growth factors (PRGFs) obtained from donors inflammatory evaluate impact health status on composition bioactivity PRGF-based treatments. Venous blood healthy volunteers patients psoriasis, atopic dermatitis processed produce PRGF supernatant. Half samples were subjected an additional thermal treatment (56 °C) inactivate immune molecules. Proteomic analysis performed assess protein PRGFs non-healthy effect Immunosafe treatment. Differential abundance patterns several proteins related key biological processes have been identified, including complement activation, coagulation, glycolysis- gluconeogenesis-related genes. These results also demonstrate (Immunosafe) contributes inactivation system and, as a consequence, reduction immunogenic potential products.
Language: Английский
Citations
4
Nutrients, Journal Year: 2024, Volume and Issue: 16(20), P. 3559 - 3559
Published: Oct. 20, 2024
Diet is one of the major determinants composition and function gut microbiome, diverse studies have established directional connections between microbiome dysbiosis skin dyshomeostasis. Furthermore, a significant link certain skin-related disorders has been reported. This work reviews mechanisms underlying relationship nutritional factors, diseases such as acne vulgaris, alopecia, atopic dermatitis. In addition, it explores how modulation human through diet various microbial strategies, including probiotics, synbiotics, postbiotics, fecal microbiota transplantation, may serve future treatments for diseases, possibly replacing traditional methods antibiotic, topical corticosteroid, laser therapies.
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 394 - 394
Published: Jan. 4, 2025
Inflammatory skin diseases comprise a group of conditions characterized by damage to function due overactive immune responses. These disorders not only impair the barrier but also deteriorate quality life and increase risk psychiatric issues. Here, low-modulus phosphatidylserine-exposing microvesicle (deformed PSV, D-PSV) was produced, characterized, evaluated for its potential therapeutic against diseases. Compared conventional PSVs (C-PSVs), D-PSVs exhibited more robust longer-lasting inhibitory effect on inflammatory response triggered lipopolysaccharides interferon-γ in primary bone marrow-derived macrophage model. Transcriptome analysis indicated that mainly achieved modulating inflammation-related signaling pathways, leading reduction expressions pro-inflammatory genes. In an imiquimod-induced psoriatic dermatitis mouse model, topical application effectively mitigated inflammation microenvironment reduced lesion severity. improvements were attributed superior permeability persistent adhesion macrophages compared with C-PSVs. summary, this macrophage-targeted offers promising non-invasive approach managing persistently inhibiting M1 polarization restoring balance.
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2544 - 2544
Published: March 12, 2025
Excessive amounts of free-oxygen radicals produced during inflammation induce oxidative stress and lead to cell damage, thus delaying the transition into proliferation in wound healing process. Oxidative on skin cells also plays an important role pathogenesis inflammatory diseases. The aim planned vitro studies was assess mechanisms regenerative action protection against three oil emulsions from transgenic (GMO) flax varieties M, B, MB a linseed emulsion traditional NIKE oil. Antioxidant gene-protective properties were identified for tested healthy model under stress. wound-healing potential these assessed proliferation, cycle, migration, apoptosis necrosis assays. conducted research presented that are safe human because they do not cancer and, at same time, migration processes normal cells. Additionally, their use increases ability eliminate damaged Transgenic oils provide effect increased antioxidant effect, resulting stress, which atopic dermatitis psoriasis. Linen B has best protective epidermis cancer, is probably due presence amount stigmasterol its composition along with appropriate content polyphenol compounds, as well oleic linoleic acids.
Language: Английский
Citations
0
Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)
Published: April 26, 2025
Abstract Chaperone proteins constitute a molecular machinery that controls proteostasis. HSPA2 is heat shock-non-inducible member of the human HSPA/HSP70 family, which includes several highly homologous chaperone proteins. exhibits cell type-specific expression pattern in testis, brain, and multilayered epithelia. It crucial male fertility-related factor, but its role somatic cells poorly understood. Previously, we found deficiency can impair epidermal keratinocyte differentiation. In this study, confirmed differentiation by investigating immortalized keratinocytes cultured reconstructed epidermis model. Moreover, uncovered influence on immunomodulation. Transcriptomic analysis revealed total loss affected genes related to interleukin- interferon-mediated signaling. The functional bidirectional changes associated with HSPA2. knockout HaCaT Ker-CT keratinocytes, not overproduction, impaired granular layer development as evidenced reduced levels late markers, filaggrin involucrin, along structural abnormalities upper layer. Differentiation defects were accompanied increased mRNA extracellular secretion keratinocyte-derived pro-inflammatory IL-6 cytokine CCL2, CCL8, CXCL1, CXCL6, CXCL10 chemokines. also led HSPA1 interferon-stimulated immune modulator SLAMF7. Knocking down decreased CCL5 release, suggesting HSPA1’s HSPA2-regulated network. To summarize, complex homeostatic keratinocytes. Our results suggest dysfunction activity could be an important pathogenicity factor potential therapeutic target for inflammatory cutaneous diseases.
Language: Английский
Citations
0
Current Obesity Reports, Journal Year: 2025, Volume and Issue: 14(1)
Published: May 13, 2025
Abstract Summary Dermatological diseases such as acne, hidradenitis suppurativa (HS), and psoriasis are driven by chronic inflammation oxidative stress. Emerging evidence highlights the role of nutrition in modulating these conditions, particularly through dietary patterns rich antioxidants, polyphenols, unsaturated fatty acids. Recent Findings The Mediterranean diet (MedDiet) has demonstrated potential benefits due to its anti-inflammatory immunomodulatory effects, while very low-energy ketogenic therapy (VLEKT) shown promise rapidly improving disease severity. Specific nutrients, including omega-3 acids, probiotics, micronutrients, may further contribute management. However, current literature is limited small-scale studies lack standardized guidelines. Purpose Review This Consensus Statement , developed collaboratively Italian Association Dietetics Clinical Nutrition (ADI), Society Dermatology Sexually Transmitted Diseases (SIDeMaST), Nutraceuticals (SINut), Club Ketodiets “KetoNut-SINut” Endocrinology (SIE), Nutrition, Hormones Metabolism aimed establish an evidence-based framework for medical (MNT) most common inflammatory skin diseases, HS psoriasis.
Language: Английский
Citations
0
Tissue and Cell, Journal Year: 2024, Volume and Issue: 91, P. 102560 - 102560
Published: Sept. 14, 2024
Language: Английский
Citations
1
Published: June 17, 2024
Inflammation is the body’s response to infection, injury, or other stimuli. Acute inflammation a crucial component of immune system’s defense, aimed at eliminating infected damaged cells and halting disease progression. A subtle interaction between pro- anti-inflammatory processes determines its progression resolution chronic inflammation. In this study, we propose generic model through system reaction-diffusion equations involving various inflammatory cytokines. We investigate formation patterns, determined by emergence Turing structures, linear stability analysis numerical simulations. These theoretical findings are further supported observations similar patterns in skin diseases.
Language: Английский
Citations
0