Altered metabolic function induced by Aβ‐oligomers and PSEN1 mutations in iPSC‐derived astrocytes DOI Creative Commons
Richard J. Elsworthy, Mattéa J. Finelli,

Sarah Aqattan

et al.

Journal of Neurochemistry, Journal Year: 2024, Volume and Issue: 169(1)

Published: Dec. 18, 2024

Abstract Altered energy metabolism in Alzheimer's disease (AD) is a major pathological hallmark implicated the early stages of process. Astrocytes play central role brain homeostasis and are multiple neurodegenerative diseases. Although numerous studies have investigated global changes metabolism, redox status, gene expression epigenetic markers AD, intricate interplay between different metabolic processes, particularly astrocytes, remains poorly understood. Numerous amyloid‐β precursor development progression AD. To determine effects peptides or impact protein processing on astrocyte we differentiated astrocytes from induced pluripotent stem cells derived people with onset familial AD controls. This study demonstrates that AD‐derived exhibit significantly more their including glucose uptake, glutamate uptake lactate release, increases oxidative glycolytic compared to acute exposure. In addition pathways glutamate, purine arginine citric acid cycle, demonstrate evidence gliosis highlighting potential hallmark. suggests chronic alterations may occur very process present significant risk factors for patients These findings also reveal important drivers late dementia highlights key targets diagnostic features therapeutic agents future. image

Language: Английский

Experimental Cell Models for Investigating Neurodegenerative Diseases DOI Open Access
Cecilia Evangelisti, Sherin Ramadan, Antonio Orlacchio

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9747 - 9747

Published: Sept. 9, 2024

Experimental models play a pivotal role in biomedical research, facilitating the understanding of disease mechanisms and development novel therapeutics. This is particularly true for neurodegenerative diseases, such as Alzheimer’s disease, Parkinson’s Huntington’s amyotrophic lateral sclerosis, motor neuron which present complex challenges research therapy development. In this work, we review recent literature about experimental disease. We identified three main categories that are highly studied by scientists. fact, investigating these diseases encompass variety approaches, including modeling patient’s cell culture, patient-derived induced pluripotent stem cells, organoids. Each model offers unique advantages limitations, providing researchers with range tools to address biological questions. Here, discuss characteristics, applications, advancements terms each system, highlighting their contributions advancing knowledge translational research.

Language: Английский

Citations

3
Altered metabolic function induced by Aβ‐oligomers and PSEN1 mutations in iPSC‐derived astrocytes DOI Creative Commons
Richard J. Elsworthy, Mattéa J. Finelli,

Sarah Aqattan

et al.

Journal of Neurochemistry, Journal Year: 2024, Volume and Issue: 169(1)

Published: Dec. 18, 2024

Abstract Altered energy metabolism in Alzheimer's disease (AD) is a major pathological hallmark implicated the early stages of process. Astrocytes play central role brain homeostasis and are multiple neurodegenerative diseases. Although numerous studies have investigated global changes metabolism, redox status, gene expression epigenetic markers AD, intricate interplay between different metabolic processes, particularly astrocytes, remains poorly understood. Numerous amyloid‐β precursor development progression AD. To determine effects peptides or impact protein processing on astrocyte we differentiated astrocytes from induced pluripotent stem cells derived people with onset familial AD controls. This study demonstrates that AD‐derived exhibit significantly more their including glucose uptake, glutamate uptake lactate release, increases oxidative glycolytic compared to acute exposure. In addition pathways glutamate, purine arginine citric acid cycle, demonstrate evidence gliosis highlighting potential hallmark. suggests chronic alterations may occur very process present significant risk factors for patients These findings also reveal important drivers late dementia highlights key targets diagnostic features therapeutic agents future. image

Language: Английский

Citations

1