Polystyrene Nanoplastics Elicit Multiple Responses in Immune Cells of the Eisenia fetida (Savigny, 1826) DOI Creative Commons

Huijian Shi,

Yaoyue Wang,

Xiangxiang Li

et al.

Toxics, Journal Year: 2024, Volume and Issue: 13(1), P. 18 - 18

Published: Dec. 26, 2024

The improper disposal of plastic products/wastes can lead to the release nanoplastics (NPs) into environmental media, especially soil. Nevertheless, their toxicity mechanisms in soil invertebrates remain unclear. This study investigated impact polystyrene NPs on Eisenia fetida (Savigny, 1826) immune cells, focusing oxidative stress, responses, apoptosis, and necrosis. Results showed that 100 nm were internalized causing cytotoxicity. observed inhibit cell viability by increasing reactive oxygen species, decreasing levels antioxidants (e.g., superoxide dismutase, catalase, glutathione), inducing lipid peroxidation DNA oxidation. Additionally, assays neutral red retention time, lysozyme activity, Ca2⁺ demonstrated resulted a loss lysosomal membrane stability reduction resistance. depolarization mitochondrial potential results apoptosis confirmed induced onset early apoptosis. difficulty NP death disrupting plasma was lactate dehydrogenase relation research provides cellular-level insights ecological risks exposure fauna.

Language: Английский

New insights into the reproductive hazards posed by polystyrene nanoplastics DOI
Mohamed Alaraby, Doaa Abass,

Antonia Velázquez

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: unknown, P. 138210 - 138210

Published: April 1, 2025

Language: Английский

Citations

0
Prenatal melatonin reprograms liver injury in male pups caused by maternal exposure to a high-fat diet and microplastics DOI Creative Commons
Yu-Jen Chen, Hong‐Ren Yu, Ching‐Chou Tsai

et al.

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Prenatal exposure to a high-fat diet (HFD) or microplastics can impact liver fat accumulation in offspring. This study investigates the protective effects of prenatal melatonin on injury male pups resulting from maternal HFD and microplastics. Pregnant Sprague-Dawley rats were fed either an normal chow diet, with some groups exposed alone combination melatonin. Male evaluated postnatal day 7. Results indicated that HFD-microplastics group (HFD-Mi) exhibited increased lipid (observed histological staining), apoptosis (elevated cleaved caspase 3, phospho-AKT, TUNEL inflammation (higher IL- 6 TNF-α), oxidative stress malondialdehyde). Conversely, treatment (HFD-Mi + M) significantly reduced these effects, including accumulation, apoptosis, inflammation, while enhancing antioxidant enzyme glutathione peroxidase activity improving metabolism (reduced SREBP- 1 expression). These findings suggest mitigates caused by through its anti-inflammatory, antioxidative, lipid-regulating properties, underscoring potential hepatoprotective role.

Language: Английский

Citations

0
Exploring Oxidative Stress and Metabolic Dysregulation in Lung Tissues of Offspring Rats Exposed to Prenatal Polystyrene Microplastics: Effects of Melatonin Treatment DOI Creative Commons
Hong‐Ren Yu, Ching‐Yi Tsai, Wei-Ling Chen

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1459 - 1459

Published: Nov. 28, 2024

Metabolomics research provides a clearer understanding of an organism’s metabolic state and enables more accurate representation its functional performance. This study aimed to investigate changes in the metabolome lung tissues resulting from prenatal exposure polystyrene microplastics (PS-MPs) understand underlying mechanisms damage rat offspring. We conducted metabolomic analyses tissue seven-day-old pups exposed PS-MPs. Our findings revealed that PS-MPs led significantly increased oxidative stress tissues, characterized by notable imbalances nucleic acid metabolism altered profiles specific amino acids. Furthermore, we evaluated therapeutic effects melatonin treatment on function 120-day-old offspring found improved histologic change affected valuable biological insights into caused exposure. Future studies should focus validating results animal experiments humans, exploring additional melatonin, developing suitable protocols for clinical use.

Language: Английский

Citations

1
Polystyrene Nanoplastics Elicit Multiple Responses in Immune Cells of the Eisenia fetida (Savigny, 1826) DOI Creative Commons

Huijian Shi,

Yaoyue Wang,

Xiangxiang Li

et al.

Toxics, Journal Year: 2024, Volume and Issue: 13(1), P. 18 - 18

Published: Dec. 26, 2024

The improper disposal of plastic products/wastes can lead to the release nanoplastics (NPs) into environmental media, especially soil. Nevertheless, their toxicity mechanisms in soil invertebrates remain unclear. This study investigated impact polystyrene NPs on Eisenia fetida (Savigny, 1826) immune cells, focusing oxidative stress, responses, apoptosis, and necrosis. Results showed that 100 nm were internalized causing cytotoxicity. observed inhibit cell viability by increasing reactive oxygen species, decreasing levels antioxidants (e.g., superoxide dismutase, catalase, glutathione), inducing lipid peroxidation DNA oxidation. Additionally, assays neutral red retention time, lysozyme activity, Ca2⁺ demonstrated resulted a loss lysosomal membrane stability reduction resistance. depolarization mitochondrial potential results apoptosis confirmed induced onset early apoptosis. difficulty NP death disrupting plasma was lactate dehydrogenase relation research provides cellular-level insights ecological risks exposure fauna.

Language: Английский

Citations

0