Cloning and characterisation of NMDA receptors in the Pacific oyster, Crassostrea gigas (Thunberg, 1793) in relation to metamorphosis and catecholamine synthesis DOI Creative Commons
Susanne Vogeler, Stefano Carboni, Xiaoxu Li

et al.

Developmental Biology, Journal Year: 2020, Volume and Issue: 469, P. 144 - 159

Published: Oct. 22, 2020

Bivalve metamorphosis is a developmental transition from free-living larva to benthic juvenile (spat), regulated by complex interaction of neurotransmitters and neurohormones such as L-DOPA epinephrine (catecholamine). We recently suggested an N-Methyl-D-aspartate (NMDA) receptor pathway additional previously unknown regulator bivalve metamorphosis. To explore this theory further, we successfully induced in the Pacific oyster, Crassostrea gigas, exposing competent larvae L-DOPA, epinephrine, MK-801 ifenprodil. Subsequently, cloned three NMDA subunits CgNR1, CgNR2A CgNR2B, with sequence analysis suggesting successful assembly functional complexes binding natural occurring agonists channel blocker MK-801. are expressed larvae, during spat, but expression neither self-regulated nor catecholamines. In-situ hybridisation CgNR1 identified presence apical organ/cerebral ganglia area potential sensory function, nervous network foot indicating putative muscle regulatory function. Furthermore, phylogenetic analyses molluscan-specific gene expansions key enzymes involved catecholamine biosynthesis. However, exposure did not alter selected enzymes, that receptors do regulate biosynthesis catecholamines via expression.

Language: Английский

Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants DOI Creative Commons
Ali Raza, Hajar Salehi, Md Atikur Rahman

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 9, 2022

Due to global climate change, abiotic stresses are affecting plant growth, productivity, and the quality of cultivated crops. Stressful conditions disrupt physiological activities suppress defensive mechanisms, resulting in stress-sensitive plants. Consequently, plants implement various endogenous strategies, including hormone biosynthesis (e.g., abscisic acid, jasmonic salicylic brassinosteroids, indole-3-acetic cytokinins, ethylene, gibberellic strigolactones) withstand stress conditions. Combined or single disrupts normal transportation solutes, causes electron leakage, triggers reactive oxygen species (ROS) production, creating oxidative Several enzymatic non-enzymatic defense systems marshal a plant’s antioxidant defenses. While responses protective role system have been well-documented recent investigations, interrelationships among hormones, neurotransmitters (NTs, such as serotonin, melatonin, dopamine, acetylcholine, γ-aminobutyric acid), defenses not well explained. Thus, this review discusses advances transgenic metabolic developments, potential interaction hormones with NTs response tolerance mechanisms. Furthermore, we discuss current challenges future directions (transgenic breeding genome editing) for improvement using modern molecular tools. The involved regulating systems, networks, abiotic-induced also discussed.

Language: Английский

Citations

208
Evolution of glutamatergic signaling and synapses DOI Creative Commons
Leonid L. Moroz, М. А. Никитин, Pavlin G. Poličar

et al.

Neuropharmacology, Journal Year: 2021, Volume and Issue: 199, P. 108740 - 108740

Published: July 31, 2021

Language: Английский

Citations

62
Whole Exome Sequencing Reveals Candidate Variants in Ion Channel Genes for Pelvic Muscle Dysfunction in Young Females with a Family History DOI
Anna Sadakierska−Chudy, Paweł Szymanowski,

Wioletta Katarzyna Szepieniec

et al.

International Urogynecology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Language: Английский

Citations

1
Glutaminase in microglia: A novel regulator of neuroinflammation DOI Creative Commons
Lu Ding,

Xiaonan Xu,

Congcong Li

et al.

Brain Behavior and Immunity, Journal Year: 2020, Volume and Issue: 92, P. 139 - 156

Published: Dec. 3, 2020

Neuroinflammation is the inflammatory responses that are involved in pathogenesis of most neurological disorders. Glutaminase (GLS) enzyme catalyzes hydrolysis glutamine to produce glutamate. Besides its well-known role cellular metabolism and excitatory neurotransmission, GLS has recently been increasingly noticed be up-regulated activated microglia under pathological conditions. Furthermore, overexpression induces microglial activation, extracellular vesicle secretion, neuroinflammatory microenvironment formation, which, compromised by inhibitors vitro vivo. These results indicate more complicated implications brain disease etiology than what previously known. In this review, we introduce isoforms, expression patterns body brain, expression/activities regulation. Next, discuss metabolic neurotransmission functions GLS. Afterwards, summarize recent findings GLS-mediated activation pro-inflammatory turns, neuroinflammation. Lastly, provide a comprehensive discussion for involvement various disorders, indicating as promising target treat these diseases.

Language: Английский

Citations

58
Relationships and Interactions between Ionotropic Glutamate Receptors and Nicotinic Receptors in the CNS DOI Creative Commons
Trevor W. Stone

Neuroscience, Journal Year: 2021, Volume and Issue: 468, P. 321 - 365

Published: June 8, 2021

Although ionotropic glutamate receptors and nicotinic for acetylcholine (ACh) have usually been studied separately, they are often co-localized functionally inter-dependent. The objective of this review is to survey the evidence interactions between two receptor families mechanisms underlying them. These include mutual regulation subunit expression, which change NMDA:AMPA response balance, existence multi-functional complexes make it difficult distinguish individual sites, especially in vivo. This followed by analysis functional relationships from work on transmitter release, cellular electrophysiology aspects behavior where these can contribute understanding interactions. It clear that (nAChRs) axonal terminals directly regulate release other neurotransmitters, α7-nAChRs generally promoting release. Hence, α7-nAChR responses will be prevented not only a antagonist, but also compounds blocking indirectly activated receptors. accounts apparent anticholinergic activity some antagonists, including endogenous antagonist kynurenic acid. activation presynaptic nAChRs ambient levels ACh released pre-terminal synapses, varicosities glial cells, acting as 'volume neurotransmitter' synaptic extrasynaptic sites. In addition, CNS co-transmitters, 'cholinergic' synapses onto spinal Renshaw cells. concluded should viewed primarily modulator glutamatergic neurotransmission regulating presynaptically, location, composition, subtype balance sensitivity receptors, classical fast neurotransmitter. conclusions caveats aid clarification sites action ligands search new centrally-acting drugs.

Language: Английский

Citations

38
Functional Organization of Extraocular Motoneurons and Eye Muscles DOI
Anja K. E. Horn, Hans Straka

Annual Review of Vision Science, Journal Year: 2021, Volume and Issue: 7(1), P. 793 - 825

Published: Sept. 15, 2021

Eye movements are indispensable for visual image stabilization during self-generated and passive head body motion orientation. muscles neuronal control elements evolutionarily conserved, with novel behavioral repertoires emerging the evolution of frontal eyes foveae. The precise execution eye different dynamics is ensured by morphologically diverse yet complementary sets extraocular muscle fibers associated motoneurons. Singly multiply innervated controlled motoneuronal subpopulations largely selective premotor inputs from task-specific ocular motor centers. morphological duality neuromuscular interface matched biochemical molecular features that collectively assign physiological properties to entities. In contrast, functionality represents a continuum where most contribute any type movement, although within preferential dynamic ranges, suggesting signal transmission contractions occur bands frequency-selective pathways.

Language: Английский

Citations

35
TDP-43 dysregulation and neuromuscular junction disruption in amyotrophic lateral sclerosis DOI Creative Commons
Sarah Lépine, María José Castellanos‐Montiel, Thomas M. Durcan

et al.

Translational Neurodegeneration, Journal Year: 2022, Volume and Issue: 11(1)

Published: Dec. 27, 2022

Amyotrophic lateral sclerosis (ALS) is a disease characterized by upper and lower motor neuron (MN) loss with signature feature of cytoplasmic aggregates containing TDP-43, which are detected in nearly all patients. Mutations the gene that encodes TDP-43 (TARBDP) known to result both familial sporadic ALS. In ALS, disruption neuromuscular junctions (NMJs) constitutes critical event pathogenesis, leading denervation atrophy, impairments disability. Morphological defects impaired synaptic transmission at NMJs have been reported several animal models vitro, linking dysregulation NMJ integrity Through lens dying-back dying-forward hypotheses this review discusses roles related function, focus on potential molecular mechanisms occurring within MNs, skeletal muscles glial cells may contribute

Language: Английский

Citations

25
The Neurotransmitters Involved in Drosophila Alcohol-Induced Behaviors DOI Creative Commons

Maggie M. Chvilicek,

Iris Titos, Adrian Rothenfluh

et al.

Frontiers in Behavioral Neuroscience, Journal Year: 2020, Volume and Issue: 14

Published: Dec. 15, 2020

Alcohol is a widely used and abused substance with numerous negative consequences for human health safety. Historically, alcohol's widespread, non-specific neurobiological effects have made it challenge to study in humans. Therefore, model organisms are critical tool unraveling the mechanisms of alcohol action subsequent on behavior.

Language: Английский

Citations

37
Sarcoglycans are enriched at the neuromuscular junction in a nerve-dependent manner DOI Creative Commons

Michela Gloriani,

Bianca Cheli,

Chiara D’Ercole

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 22, 2025

Language: Английский

Citations

0
Vesicle‐released glutamate is necessary to maintain muscle spindle afferent excitability but not dynamic sensitivity in adult mice DOI Open Access

Kimberly Than,

Enoch Kim,

Cebrina Navarro

et al.

The Journal of Physiology, Journal Year: 2021, Volume and Issue: 599(11), P. 2953 - 2967

Published: March 22, 2021

Muscle spindle afferents are slowly adapting low threshold mechanoreceptors that report muscle length and movement information critical for motor control proprioception. The rapidly cation channel PIEZO2 has been identified as necessary afferent stretch sensitivity, although the properties of this suggest additional molecular elements mediating complex response afferents. We glutamate increases static sensitivity in an ex vivo mouse nerve preparation, blocking packaging into vesicles by sole vesicular transporter, VGLUT1, either pharmacologically or transgenic knockout one allele VGLUT1 decreases but not dynamic sensitivity. Our results confirm vesicle-released is important contributor to maintained excitability may a therapeutic target normalizing function.Muscle have both stretch. exact mechanism which these neurons translate action potentials well understood, mechanically sensitive essential adapting, suggesting requirement maintain firing during Spindle sensory endings contain glutamate-filled synaptic-like released stretch- calcium-dependent manner. Previous work shown can increase phospholipase-D coupled metabotropic antagonist abolish Here, we test hypothesis maintaining To hypothesis, used muscle-nerve preparation measure responses vibration. In C57BL/6 adult mice, bath applied significantly increased rate plateau phase Blocking with xanthurenic acid using only copy gene (VGLUT1+/- ), decreased sustained model mechanotransduction where calcium entering cause release from vesicles, then helps depolarization firing.

Language: Английский

Citations

26