Microalgae as an Efficient Vehicle for the Production and Targeted Delivery of Therapeutic Glycoproteins against SARS-CoV-2 Variants DOI Creative Commons
Jaber Dehghani, Ali Movafeghi, Elodie Mathieu‐Rivet

et al.

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(11), P. 657 - 657

Published: Oct. 23, 2022

Severe acute respiratory syndrome–Coronavirus 2 (SARS-CoV-2) can infect various human organs, including the respiratory, circulatory, nervous, and gastrointestinal ones. The virus is internalized into cells by binding to angiotensin-converting enzyme (ACE2) receptor through its spike protein (S-glycoprotein). As S-glycoprotein required for attachment entry target cells, it primary mediator of SARS-CoV-2 infectivity. Currently, this glycoprotein has received considerable attention as a key component development antiviral vaccines or biologics against SARS-CoV-2. Moreover, since ACE2 constitutes main route virus, soluble form could be considered promising approach treatment coronavirus disease 2019 infection (COVID-19). Both are highly glycosylated molecules containing 22 7 consensus N-glycosylation sites, respectively. N-glycan structures attached these specific sites folding, conformation, recycling, biological activity both glycoproteins. Thus far, recombinant have been produced primarily in mammalian which an expensive process. Therefore, benefiting from cheaper cell-based biofactory would good value added cost-effective biopharmaceuticals directed COVID-19. To end, efficient synthesis machinery ability properly impose post-translational modifications make microalgae eco-friendly platform production pharmaceutical Notably, several (e.g., Chlamydomonas reinhardtii, Dunaliella bardawil, Chlorella species) already approved U.S. Food Drug Administration (FDA) safe food. Because microalgal contain rigid cell wall that act natural encapsulation protect proteins aggressive environment stomach, feature used rapid edible targeted delivery treatment/inhibition Herein, we reviewed pathogenesis mechanism then highlighted potential COVID-19 infection.

Language: Английский

Production and secretion of functional SARS-CoV-2 spike protein in Chlamydomonas reinhardtii DOI Creative Commons
Anna Maria Kiefer, Justus Niemeyer,

Anna Probst

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Sept. 20, 2022

The spike protein is the major on surface of coronaviruses. It therefore prominent target neutralizing antibodies and consequently antigen all currently admitted vaccines against SARS-CoV-2. Since it a 1,273-amino acids glycoprotein with 22 N-linked glycans, production functional, full-length was limited to higher eukaryotes. Here we report SARS-CoV-2 - lacking C-terminal membrane anchor as secreted in prefusion-stabilized conformation unicellular green alga Chlamydomonas reinhardtii. We show that efficiently cleaved at furin cleavage site during synthesis abolished upon mutation multi-basic site. could enrich from culture medium by ammonium sulfate precipitation demonstrate its functionality based interaction recombinant ACE2 expressed human 293T cells. reinhardtii GRAS organism can be cultivated low cost simple media large scale, making an attractive platform for other biopharmaceuticals low-income countries.

Language: Английский

Citations

26
Harnessing the Algal Chloroplast for Heterologous Protein Production DOI Creative Commons
Edoardo Andrea Cutolo, Giulia Mandalà, Luca Dall’Osto

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(4), P. 743 - 743

Published: March 30, 2022

Photosynthetic microbes are gaining increasing attention as heterologous hosts for the light-driven, low-cost production of high-value recombinant proteins. Recent advances in manipulation unicellular algal genomes offer opportunity to establish engineered strains safe and viable alternatives conventional heterotrophic expression systems, including their use feed, food, biopharmaceutical industries. Due relatively small size genomes, chloroplasts excellent targets synthetic biology approaches, convenient subcellular sites compartmentalized accumulation storage products. Different classes proteins, enzymes peptides with therapeutical applications, have been successfully expressed plastid model organism Chlamydomonas reinhardtii, a few other species, highlighting emerging potential transplastomic biotechnology. In this review, we provide unified view on state-of-the-art tools that available introduce protein-encoding transgenes microalgal plastids, discuss main (bio)technological bottlenecks still need be addressed develop robust sustainable green cell biofactories.

Language: Английский

Citations

25
Molecular design of microalgae as sustainable cell factories DOI Creative Commons
Alexander Einhaus, Thomas Baier, Olaf Kruse

et al.

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 42(6), P. 728 - 738

Published: Dec. 12, 2023

Microalgae are regarded as sustainable and potent chassis for biotechnology. Their capacity efficient photosynthesis fuels dynamic growth independent from organic carbon sources converts atmospheric CO

Language: Английский

Citations

14
Generation of microalga Chlamydomonas reinhardtii expressing VP28 protein as oral vaccine candidate for shrimps against White Spot Syndrome Virus (WSSV) infection DOI
Pham Thi Lanh, Huong Minh Nguyen,

Bùi Thị Thùy Dương

et al.

Aquaculture, Journal Year: 2021, Volume and Issue: 540, P. 736737 - 736737

Published: April 5, 2021

Language: Английский

Citations

29
A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga DOI Creative Commons
Vivien Hotter, David Zopf, Hak Joong Kim

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(33)

Published: Aug. 13, 2021

Significance Algae live in association with microbes that interact by a variety of chemical mediators, resulting mutualistic or antagonistic relationships. Although algae are key contributors to carbon fixation and fundamental for food webs, we still know little about the underlying molecular mechanisms affecting their fitness. This study investigates interaction between an bacterium unicellular alga. It demonstrates multiple roles polyyne, protegencin, is used bacteria attack green algal cells. highly effective toxin alters subcellular compartment vision, bleaches, lyses These results expand our knowledge arsenal mediators modes action communities.

Language: Английский

Citations

27
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae DOI
Eva YuHua Kuo,

Ru‐Yin Yang,

Yuan Yu Chin

et al.

Biotechnology Journal, Journal Year: 2022, Volume and Issue: 17(8)

Published: April 25, 2022

Abstract Microalgae, a group of photosynthetic microorganisms rich in diverse and novel bioactive metabolites, have been explored for the production biofuels, high value‐added compounds as food feeds, pharmaceutical chemicals agents with therapeutic benefits. This article reviews development omics resources genetic engineering techniques including gene transformation methodologies, mutagenesis, genome‐editing tools microalgae biorefinery wastewater treatment (WWT). The introduction these enlisted has simplified understanding complex metabolic pathways undergoing microalgal cells. multiomics approach integrated datasets, big data analysis, machine learning discovery objective traits genes responsible was reviewed. Recent advances limitations analysis bioengineering technology to facilitate improvement dual role WWT feedstock are discussed.

Language: Английский

Citations

21
Molecular Advancements Establishing Chlamydomonas as a Host for Biotechnological Exploitation DOI Creative Commons
Michael Schroda, Claire Remacle

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 29, 2022

Chlamydomonas reinhardtii is emerging as a production platform for biotechnological purposes thanks to recent achievements, which we briefly summarize in this review. Firstly, robust nuclear transgene expression now possible because several impressive improvements have been made years. Strains allowing efficient and stable are available were recently more amenable rational approaches by enabling genetic crosses identifying their causative mutation. The MoClo synthetic biology strategy, based on Golden Gate cloning, was developed includes growing toolkit of than 100 parts that can be robustly rapidly assembled predefined order. This allows rapid iterative cycles design, building, testing, learning. Another major advancement came from various findings improving design such the systematic addition introns into codon-optimized coding sequences. Lastly, CRISPR/Cas9 technology genome editing has undergone since its first successful report 2016, opens possibility optimizing biosynthetic pathways switching off competing ones. We provide few examples demonstrating all these developments firmly establish chassis allow rewiring metabolism new capabilities.

Language: Английский

Citations

21
Optimized production of a bioactive human recombinant protein from the microalgae Chlamydomonas reinhardtii grown at high density in a fed-batch bioreactor DOI Creative Commons
Yasin Torres-Tiji, Francis J. Fields, Yanping Yang

et al.

Algal Research, Journal Year: 2022, Volume and Issue: 66, P. 102786 - 102786

Published: July 1, 2022

Microalgae have been identified as an alternative platform to produce high-quality biomass and subsequent bioproducts, such foods, feeds, nutritional supplements, recombinant proteins, biofuels. Traditional biotechnological hosts for therapeutic the bacteria Escherichia coli mammalian CHO cells, long established dominate platforms, but recent advances shown that microalgae can potentially serve platform. In present study, we examine potential of microalga Chlamydomonas reinhardtii a complex human protein in high-density heterotrophic culture. The protein, ICAM-1, was targeted secretion extracellular media culture from cells grown bioreactor using fed-batch strategy achieve high cell density. Ultimately, this resulted maximum titer 40 g/L 50 mg/L. algal-produced ICAM-1 showed comparable bioactivity produced measured binding assays its native ligand LFA-1. This work shows C. is viable option with biological activity, at concentrations fed batch growth strategy.

Language: Английский

Citations

19
A Rapid and Reversible Molecular “Switch” Regulating Protein Expression in Chlamydomonas reinhardtii DOI Open Access

Xinyi Li,

Song Wang,

Qianyi Li

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Chlamydomonas reinhardtii, a prominent chassis in synthetic biology, faces limitations regulating the expression of exogenous genes. A destabilization domain (DD)/Shield-1 system, originally derived from mammals, offers ligand-dependent control stability, making it valuable tool. This system utilises to induce rapid degradation target protein unless stabilised by Shield-1, ligand. Upon addition Shield-1,the is halted, leading accumulation and stabilisation protein. has demonstrated successful regulation foreign parasites, plants. In this study, DD/Shield-1 was harnessed regulate paromomycin resistance gene luciferase encoding Chlamydomonas, revealing its capability for rapid, stable, reversible microalgae, serving as molecular switch. Furthermore, exhibits reagent dependency, enhancing applicability practical production. strain with induced gene-editing protein, LbCas12a, successfully constructed then tested editing. The findings not only enrich toolkit studies but offer promising technique validating functionality proteins microalgae.

Language: Английский

Citations

0
Oral immunization of grouper with recombinant Chlamydomonas to prevent viral nervous necrosis and the effect of which on intestinal microbiota DOI
Xiaodong Deng, Donghua Guo, Xiaoxing Cheng

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 41, P. 102685 - 102685

Published: Feb. 15, 2025

Language: Английский

Citations

0