Microalgae as expression systems for recombinant protein production DOI Creative Commons
Luis Muñoz-Solórzano, Kate Willis-Ureña, Sebastián Valverde-Rojas

et al.

Revista Tecnología en Marcha, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 29, 2024

In the field of biotechnology, recombinant proteins have revolutionized many industries, including pharmaceuticals, agriculture, and bioenergy. By producing high-value in heterologous hosts, cell factories may offer a more efficient, cost-effective, scalable, environmentally friendly solution to traditional protein production extraction methods, which can be highly laborious resource intensive. Microalgae emerged as attractive hosts due their Generally Recognized Safe (GRAS) status, versatile metabolism, genetic diversity between species, ease cultivation scale-up, general cost-effectiveness. For engineering, capability for complex synthesis post-translational modifications transformation comparison with chasses outside category make microalgae an advantageous on fronts. transformed enable efficient expression, most commonly nucleus chloroplast, each specific advantages limitations. The present literature review compiles some techniques, features, latest advances related microalgae, exploring different techniques Recombinant is only one processes that become sustainable by using platform.

Language: Английский

Chlamydomonas reinhardtii: A Factory of Nutraceutical and Food Supplements for Human Health DOI Creative Commons
Annalisa Masi, Francesca Leonelli, Viviana Scognamiglio

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(3), P. 1185 - 1185

Published: Jan. 25, 2023

Chlamydomonas reinhardtii (C. reinhardtii) is one of the most well-studied microalgae organisms that revealed important information for photosynthetic and metabolic processes plants eukaryotes. Numerous extensive studies have also underpinned its great potential as a biochemical factory, capable producing various highly desired molecules with direct impact on human health longevity. Polysaccharides, lipids, functional proteins, pigments, hormones, vaccines, antibodies are among valuable biomolecules produced spontaneously or under well-defined conditions by C. can be directly linked to nutrition diet. The aim this review highlight recent advances in field focusing relevant applications related production limitations challenges critically discussed along future biomass processed products nutraceuticals food supplements. increasing need high-value low-cost an environmentally economy sustainable manner underline role reinhardtii.

Language: Английский

Citations

39
Genetically engineered microalgae for enhanced bioactive compounds DOI Creative Commons
Ahmad Hanafi Ahmad Kamal,

Nor Farahdevah Mohd Hamidi,

Muhammad Faiz Zakaria

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(9)

Published: Sept. 9, 2024

Language: Английский

Citations

8
The role of proline in the adaptation of eukaryotic microalgae to environmental stress: An underestimated tool for the optimization of algal growth DOI Creative Commons
Simone Barera, Giuseppe Forlani

Journal of Applied Phycology, Journal Year: 2023, Volume and Issue: 35(4), P. 1635 - 1648

Published: June 24, 2023

Abstract Microalgae are considered the most promising source of renewable fuels, high-value bio-products and nutraceuticals. Potentially, microalgae can satisfy many global demands, but in large-scale cultivation average productivity industrial strains is lower than maximal theoretical estimations, mainly due to sub-optimal growth conditions. Although have developed complex strategies cope with environmental stresses, outdoor photobioreactors limited few species it not yet sufficiently remunerative. Indeed, microalgal very sensitive conditions, changes solar irradiation, temperature, medium composition drastically decrease biomass yield. Developing new for improving algal tolerance stress conditions thus greatly desirable. One first responses that occur both higher plants microorganisms following exposure abiotic an increased synthesis accumulation amino acid proline. While role proline adaptation well-recognized plants, implication still awaits full elucidation. In this review we summarize available data on metabolism under eukaryotic microalgae. Possible implications toward optimization biotechnological purposes also discussed.

Language: Английский

Citations

14
Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae DOI Creative Commons
Thamali Kariyawasam,

Christian Helvig,

Martin Petkovich

et al.

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(6)

Published: June 1, 2024

Pharmaceuticals are of increasing environmental concern as they emerge and accumulate in surface- groundwater systems around the world, endangering overall health aquatic ecosystems. Municipal wastewater discharge is a significant vector for pharmaceuticals their metabolites to enter surface waters humans incompletely absorb prescription drugs excrete up 50% into wastewater, which subsequently removed during treatment. Microalgae present promising target improving treatment due ability remove some pollutants efficiently. However, inherent metabolic pathways limit capacity degrade more recalcitrant organic compounds such pharmaceuticals. The human liver employs enzymes break down drugs, these extensively researched drug development, meaning cytochrome P450 responsible metabolizing each approved well studied. Thus, unlocking or expression endogenous microalgae could be cost-effective strategy reduce pharmaceutical loads effluents. Here, we discuss challenges opportunities associated with introducing microalgae. We anticipate that P450-engineered can serve new removal method sustainable solution upgrade facilities function "mega livers".

Language: Английский

Citations

4
Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia DOI Creative Commons
Edoardo Andrea Cutolo, Rosanna Campitiello, Roberto Caferri

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(7), P. 304 - 304

Published: June 28, 2024

From sea shores to the abysses of deep ocean, marine ecosystems have provided humanity with valuable medicinal resources. The use organisms is discussed in ancient pharmacopoeias different times and geographic regions still deeply rooted traditional medicine. Thanks present-day, large-scale bioprospecting rigorous screening for bioactive metabolites, ocean coming back as an untapped resource natural compounds therapeutic potential. This renewed interest drugs propelled by a burgeoning research field investigating molecular mechanisms which newly identified intervene pathophysiology human diseases. Of great clinical relevance are molecules endowed anti-inflammatory immunomodulatory properties emerging applications management chronic inflammatory disorders, autoimmune diseases, cancer. Here, we review historical development pharmacology Eastern Western worlds describe status drug discovery. Finally, discuss importance conducting sustainable exploitation resources through biotechnology.

Language: Английский

Citations

4
Enhancing Microalgal Proteins for Nutraceutical and Functional Food Applications DOI Creative Commons
Manpreet Kaur, Surekha Bhatia, Debasis Bagchi

et al.

Future Foods, Journal Year: 2025, Volume and Issue: 11, P. 100564 - 100564

Published: Feb. 6, 2025

Language: Английский

Citations

0
Marine Phytoplankton Bioactive Lipids and Their Perspectives in Clinical Inflammation DOI Creative Commons
Edoardo Andrea Cutolo, Rosanna Campitiello, Valeria Di Dato

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 86 - 86

Published: Feb. 17, 2025

Marine phytoplankton is an emerging source of immunomodulatory bioactive lipids (BLs). Under physiological growth conditions and upon stress challenges, several eukaryotic microalgal species accumulate lipid metabolites that resemble the precursors animal mediators inflammation: eicosanoids prostaglandins. Therefore, marine could serve as a biotechnological platform to produce functional BLs with therapeutic applications in management chronic inflammatory diseases other clinical conditions. However, be commercially competitive, lipidic precursor yields should enhanced. Beside tailoring cultivation native producers, genetic engineering feasible strategy accrue production introduce heterologous biosynthetic pathways hosts. Here, we present state-of-the-art research on from discuss synthetic biology approaches boost their light-driven biosynthesis.

Language: Английский

Citations

0
In Vitro Culture, Genetic Transformation and the Production of Biopharmaceuticals in Microalgae DOI Open Access
Aneta Gerszberg, Ludmiła Kolek, Katarzyna Hnatuszko-Konka

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3890 - 3890

Published: April 20, 2025

Microalgae represent a promising platform for the synthesis of recombinant proteins, particularly in context biopharmaceutical applications. Their unique combination eukaryotic cellular machinery and prokaryotic-like simplicity offers several advantages, including ability to perform complex post-translational modifications, rapid growth rates, cost-effective culture conditions. Advances genome sequencing, genetic engineering tools, omics technologies have significantly enhanced feasibility efficiency using microalgae therapeutic protein production. These advancements, coupled with development well-established transformation methods optimized vectors, enabled successful expression various biopharmaceuticals, ranging from vaccines enzymes. Here, main stages current status production exogenic proteins dedicated human therapy are presented.

Language: Английский

Citations

0
Effects of oral delivery of two antimicrobial peptides CATHPb1 and As-CATH4 by bait microalga [Tetraselmis subcordiformis (Wille) Butcher] on resistance to bacterial infection in turbot (Scophthalmus maximus L.) DOI

Subing Han,

Yaping Shao,

Ping Liu

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102803 - 102803

Published: May 1, 2025

Language: Английский

Citations

0
Chlamydomonas: Fast tracking from genomics DOI Creative Commons
Justin Findinier, Arthur Grossman

Journal of Phycology, Journal Year: 2023, Volume and Issue: 59(4), P. 644 - 652

Published: July 7, 2023

Abstract Elucidating biological processes has relied on the establishment of model organisms, many which offer advantageous features such as rapid axenic growth, extensive knowledge their physiological and gene content, ease with they can be genetically manipulated. The unicellular green alga Chlamydomonas reinhardtii been an exemplary that enabled scientific breakthroughs over decades, especially in fields photosynthesis, cilia function biogenesis, acclimation photosynthetic organisms to environment. Here, we discuss recent molecular/technological advances have applied C. how further fostered its development a “flagship” algal system. We also explore future promise this leveraging genomics, proteomics, imaging, synthetic biology for addressing critical issues.

Language: Английский

Citations

8