In vitro investigation of epigenetic regulators related to chemo-resistance and stemness of CD133+VE cells sorted from U87MG cell line

Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149432 - 149432

Published: March 1, 2025

Language: Английский

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Biomolecules to Biomarkers? U87MG Marker Evaluation on the Path towards Glioblastoma Multiforme Pathogenesis

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(1), P. 123 - 123

Published: Jan. 18, 2024

The heterogeneity of the glioma subtype glioblastoma multiforme (GBM) challenges effective neuropathological treatment. reliance on in vitro studies and xenografted animal models to simulate human GBM has proven ineffective. Currently, a dearth knowledge exists regarding applicability cell line biomolecules realm pathogenesis. Our study’s objectives were address this preclinical issue assess prominin-1, ICAM-1, PARTICLE GAS5 as potential diagnostic targets. methodologies included haemoxylin eosin staining, immunofluorescence, situ hybridization quantitative PCR. findings identified that morphology correlates with malignancy patient pathology. Immunofluorescence confocal microscopy revealed prominin-1 pseudo-palisades adjacent necrotic foci both GBM. Evidence is presented for an ICAM-1 association degenerating vasculature. Significantly elevated nuclear expression from PCR reflected its role tumor activator. within validated prognostic biomolecule extended survival. Here we present evidence stem marker chemotherapeutic target model pathology sections patients elicited alternative responses adjuvant chemotherapy. This foremost study introduces long non-coding RNA into context pathogenesis while substantiating suppressor. validation biomarkers cellular contributes advancement towards superior detection, therapeutic responders ultimate attainment promising prognoses currently incurable brain cancer.

Language: Английский

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Targeting Non-coding RNA for Glioblastoma Therapy: The Challenge of Overcomes the Blood-Brain Barrier

Frontiers in Medical Technology, Journal Year: 2021, Volume and Issue: 3

Published: Aug. 10, 2021

Glioblastoma (GBM) is the most malignant form of all primary brain tumors, and it responsible for around 200,000 deaths each year worldwide. The standard therapy GBM treatment includes surgical resection followed by temozolomide-based chemotherapy and/or radiotherapy. With this treatment, median survival rate patients only 15 months after its initial diagnosis. Therefore, novel better modalities are urgently needed. Mounting evidence indicates that non-coding RNAs (ncRNAs) have critical roles as regulators gene expression. Long (lncRNAs) microRNAs (miRNAs) among studied ncRNAs in health disease. Dysregulation observed virtually tumor types, including GBMs. Several dysregulated miRNAs lncRNAs been identified cell lines samples. Some them proposed diagnostic prognostic markers, targets treatment. Most ncRNA-based therapies use oligonucleotide RNA molecules which normally short life circulation. Nanoparticles (NPs) designed to increase half-life RNAs. An additional challenge faced not oligonucleotides but brain-related conditions, presence blood-brain barrier (BBB). BBB anatomical protects from undesirable agents. Although some NPs derivatized at their surface cross BBB, optimal deliver into cells currently unavailable. In review, we describe first current treatments therapy. Next, discuss relevant suggested Then, compare drug delivery systems (nanocarriers/NPs) delivery, challenges send drugs through strategies overcome barrier. Finally, categorize points where research should be focus order design brain; thus move Oligonucleotide RNA-based bench clinical setting.

Language: Английский

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LncRNAs as Therapeutic Targets and Potential Biomarkers for Lipid-Related Diseases

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 4, 2021

Lipid metabolism is an essential biological process involved in nutrient adjustment, hormone regulation, and lipid homeostasis. An irregular lifestyle long-term overload can cause lipid-related diseases, including atherosclerosis, myocardial infarction (MI), obesity, fatty liver diseases. Thus, novel tools for efficient diagnosis treatment of dysfunctional are urgently required. Furthermore, it known that lncRNAs based regulation like sponging microRNAs (miRNAs) or serving as a reservoir play role the progression Accordingly, better understanding regulatory roles diseases would provide basis identifying potential biomarkers therapeutic targets This review highlighted latest advances on summarised current knowledge dysregulated their molecular mechanisms. We have also provided insights into underlying mechanisms which might serve The information presented here may be useful designing future studies advancing investigations diagnosis, prognosis, therapy

Language: Английский

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LncIMF2 promotes adipogenesis in porcine intramuscular preadipocyte through sponging MiR-217

Animal Biotechnology, Journal Year: 2021, Volume and Issue: 34(2), P. 268 - 279

Published: Aug. 4, 2021

Intramuscular fat is positively related to meat quality including tenderness, flavor, and juiciness. Long noncoding RNA (LncRNA) plays a vital role in regulating adipogenesis. However, it largely unknown about lncRNAs associated with porcine intramuscular adipocyte In the present study, we focus on novel LncRNA, which named lncIMF2, adipogenesis by our previous RNA-sequence analysis bioinformatics analysis. We demonstrated LncIMF2 knockdown inhibited proliferation of adipocytes while expression cell cycle-related genes was decreased. Besides, found adipogenic differentiation marker PPARγ (Peroxisome proliferator-activated reporter gamma) ATGL (Adipose triglyceride lipase). Similarly, overexpression promotes preadipocytes. Moreover, proved that IncIMF2 acts as molecular sponge for MicroRNA-217 (miR-217), has been adipogenesis, thereby affecting miR-217 target gene. Collectively, findings will contribute deeper understanding LncRNA pig IMF deposition improvement quality.

Language: Английский

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MIR4435-2HG: A newly proposed lncRNA in human cancer

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 150, P. 112971 - 112971

Published: April 18, 2022

Long non-coding RNAs (lncRNAs) play important roles in the occurrence and progression of tumors. Extensive research has contributed to current understanding critical played by lncRNAs various cancers. LncRNA MIR4435-2HG been found be crucial many cancers, such as breast, cervical, colorectal, gastric cancer. Expression is generally upregulated cancers participates biological functions through molecular mechanism competitive endogenous RNA networks. This review profiles recent findings on expression, functions, mechanism, clinical value cancer, serves a reference for further MIR4435-2HG-related trials.

Language: Английский

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Non-coding RNAs: The recently accentuated molecules in the regulation of cell autophagy for ovarian cancer pathogenesis and therapeutic response

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: March 31, 2023

Autophagy is a self-recycling and conserved process, in which the senescent cytoplasmic components are degraded cells then recycled to maintain homeostatic balance. Emerging evidence has suggested involvement of autophagy oncogenesis progression various cancers, such as ovarian cancer (OC). Meanwhile, non-coding RNAs (ncRNAs) frequently regulate mRNA transcription other functional signaling pathways cell autophagy, displaying promising roles human pathogenesis therapeutic response. This article mainly reviews cutting-edge research advances about interactions between ncRNAs OC. review not only summarizes underlying mechanisms dynamic ncRNA-autophagy association OC, but also discusses their prognostic implications biomarkers. The aim this was provide more in-depth knowledge framework exploring crosstalk highlight treatment strategies for OC patients.

Language: Английский

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Mechanisms of Long Non-Coding RNAs in Biological Characteristics and Aerobic Glycolysis of Glioma

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(20), P. 11197 - 11197

Published: Oct. 18, 2021

Glioma is the most common and aggressive tumor of central nervous system. The uncontrolled proliferation, cellular heterogeneity, diffusive capacity glioma cells contribute to a very poor prognosis patients with high grade glioma. Compared normal cells, cancer exhibit higher rate glucose uptake, which accompanied metabolic switch from oxidative phosphorylation aerobic glycolysis. reprogramming cell supports excessive are frequently mediated by activation oncogenes or perturbations suppressor genes. Recently, growing body evidence has started reveal that long noncoding RNAs (lncRNAs) implicated in wide spectrum biological processes glioma, including malignant phenotypes However, mechanisms diverse lncRNAs initiation progression gliomas remain be fully unveiled. In this review, we summarized roles shaping features glycolysis thorough understanding biology provides opportunities for developing diagnostic biomarkers novel therapeutic strategies targeting gliomas.

Language: Английский

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An in vivo model of glioblastoma radiation resistance identifies long noncoding RNAs and targetable kinases

JCI Insight, Journal Year: 2022, Volume and Issue: 7(16)

Published: July 19, 2022

Key molecular regulators of acquired radiation resistance in recurrent glioblastoma (GBM) are largely unknown, with a dearth accurate preclinical models. To address this, we generated 8 GBM patient-derived xenograft (PDX) models therapy-selected (RTS) compared same-patient, treatment-naive (radiation-sensitive, unselected; RTU) PDXs. These likely unique mimic the longitudinal evolution patient tumors following serial therapy. Indeed, while whole-exome sequencing showed retention major genomic alterations RTS lines, did detect chromosome 12q14 amplification that was associated clinical recurrence 2 A potentially novel bioinformatics pipeline applied to analyze phenotypic, transcriptomic, and kinomic alterations, which identified long noncoding RNAs (lncRNAs) targetable, PDX-specific kinases. We observed differential transcriptional enrichment DNA damage repair pathways our models, correlated several lncRNAs. Global profiling separated RTU but pairwise analyses indicated there multiple routes resistance. model-specific kinases were targeted clinically relevant small molecule inhibitors. This cohort vivo will enable future therapeutic testing help overcome treatment seen patients GBM.

Language: Английский

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Prognostic utility of lncRNAs (LINC00565 and LINC00641) as molecular markers in glioblastoma multiforme (GBM)

Journal of Neuro-Oncology, Journal Year: 2022, Volume and Issue: 158(3), P. 435 - 444

Published: June 6, 2022

Glioblastoma multiforme (GBM) is primary brain tumor grade IV characterized by fast cell proliferation, high mortality and morbidity most lethal gliomas. Molecular approaches underlying its pathogenesis progression with diagnostic prognostic value have been an area of interest. Long-non coding RNAs (lncRNAs) aberrantly expressed in GBM recently studied. The aim to investigate the clinical role lncRNA565 lncRNA641 patients.Blood samples were withdrawn from 35 newly diagnosed cases 15 healthy individuals, then expression evaluated using real time-PCR. Their efficacy was detected receiver operating characteristic curve. Progression free survival (PFS) overall (OS) studied Kaplan-Meier curves.lncRNAs expressions increased significantly among as compared control group. correlated clinico-pathological data pattern for patients. Higher levels both lncRNAs worse performance status. Expression large size (≥ 5 cm). Survival analysis showed that investigated lncRNA PFS OS.Expression a liquid biopsy sample can be used biomarker

Language: Английский

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