Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Micromachines, Journal Year: 2023, Volume and Issue: 14(12), P. 2217 - 2217

Published: Dec. 8, 2023

The present work is a review of the research on using hydrogels based natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This addresses main properties starch that make them promising materials in biotechnology producing preparations stable during use storage insensitive to chemical physical impacts. authors summarize their achievements developing enzymes immobilized gels assess activity, stability, sensitivity as biorecognition elements inhibition-based biosensors.

Language: Английский

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NIR-Sensitive Squaraine Dye—Peptide Conjugate for Trypsin Fluorogenic Detection

Biosensors, Journal Year: 2024, Volume and Issue: 14(10), P. 458 - 458

Published: Sept. 25, 2024

Trypsin enzyme has gained recognition as a potential biomarker in several tumors, such colorectal, gastric, and pancreatic cancer, highlighting its importance disease diagnosis. In response to the demand for rapid, cost-effective, real-time detection methods, we present an innovative strategy utilizing design synthesis of NIR-sensitive dye-peptide conjugate (

Language: Английский

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Alternative Enzyme Inhibition Assay for Safety Evaluation of Food Preservatives

Life, Journal Year: 2023, Volume and Issue: 13(6), P. 1243 - 1243

Published: May 24, 2023

While food additives are widely used in the modern industry and generally important maintaining ability to provide for increasing world population, progress occurring this field is much ahead of evaluation their possible consequences human health. The present study suggests a set single- multi-enzyme assay systems revealing toxic effects most spread preservatives, such as sorbic acid (E200), potassium sorbate (E202), sodium benzoate (E211) at primary molecular level interaction with enzymes. based on inhibition enzyme activity by substances proportional amount toxicants sample. single-enzyme system NAD(P)H:FMN oxidoreductase (Red) proved be sensitive impact additives, IC50 values being 29, 14, 0.02 mg/L benzoate, sorbate, acid, respectively, which considerably lower than acceptable daily intake (ADI). No reliable change degree preservatives was observed upon elongating series coupled redox reactions. However, 50% found preservative concentration below maximum permissible food. effect butyrylcholinesterase (BChE), lactate dehydrogenase (LDH), alcohol (ADH) either absent or presence concentrations significantly exceeding ADI. Among under study, considered safest terms inhibiting activity. results show that negative organization living things highly pronounced, while organismal it may not obvious.

Language: Английский

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Advanced Techniques for the Analysis of Proteins and RNAs

Chemosensors, Journal Year: 2024, Volume and Issue: 12(1), P. 12 - 12

Published: Jan. 10, 2024

Proteins and RNAs, as fundamental components of cellular machinery, play pivotal roles in the intricate landscape life [...]

Language: Английский

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Advanced Biosensors: Electrochemical Impedance Study of Hybrid Materials Based on Layered Double Hydroxide/Trypsin

Chemistry Africa, Journal Year: 2024, Volume and Issue: 7(10), P. 5659 - 5668

Published: Nov. 18, 2024

Language: Английский

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