NeuM: A Neuon‐Selective Probe Incorporates into Live Neuronal Membranes via Enhanced Cathrin‐Mediated Endocytosis in Primary Neurons. DOI Creative Commons

Yoonsik Sung,

Lizaveta Gotina,

Kyu Hyeon Kim

et al.

Angewandte Chemie, Journal Year: 2023, Volume and Issue: 136(3)

Published: Dec. 8, 2023

Abstract The development of a small‐molecule probe designed to selectively target neurons would enhance the exploration intricate neuronal structures and functions. Among such probes, NeuO stands out as pioneer has gained significant traction in field research. Nevertheless, neither mechanism behind neuron‐selectivity nor cellular localization been determined. Here, we introduce NeuM, derivative NeuO, cell membranes. Furthermore, elucidate selective membrane trafficking that distinguishes neurons. In an aqueous buffer, NeuM autonomously assembles into micellar structures, leading quenching its fluorescence (Φ=0.001). Upon exposure neurons, micelles were internalized endosomes via clathrin‐mediated endocytosis. Through endocytic recycling pathway, integrate membrane, dispersing fluorescent molecules (Φ=0.61). Molecular dynamics simulations demonstrated comparison possesses optimal lipophilicity molecular length, facilitating stable incorporation phospholipid layers. integration within allows prolonged monitoring well visualization structures.

Language: Английский

AI-driven precision subcellular navigation with fluorescent probes DOI
Yingli Zhu,

Yanpeng Fang,

Wenzhi Huang

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(43), P. 11054 - 11062

Published: Jan. 1, 2024

Precise navigation within intricate biological systems is pivotal for comprehending cellular functions and diagnosing diseases. Fluorescent molecular probes, designed to target specific molecules, are indispensable tools this endeavor. This paper delves into the revolutionary potential of artificial intelligence (AI) in crafting highly precise effective fluorescent probes. We will discuss how AI can be employed to: design new subcellular dyes by optimizing physicochemical properties; prospective targeting probes based on receptors; quantitatively explore chemical laws molecules optimize optical properties probes; comprehensive probe guide construction multifunctional Additionally, we showcase recent AI-driven advancements development their successful biomedical applications, while addressing challenges outlining future directions towards transforming research, diagnostics, drug discovery.

Language: Английский

Citations

4
NeuM: A Neuron‐Selective Probe Incorporates into Live Neuronal Membranes via Enhanced Clathrin‐Mediated Endocytosis in Primary Neurons DOI Creative Commons

Yoonsik Sung,

Lizaveta Gotina,

Kyu Hyeon Kim

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 63(3)

Published: Dec. 8, 2023

The development of a small-molecule probe designed to selectively target neurons would enhance the exploration intricate neuronal structures and functions. Among such probes, NeuO stands out as pioneer has gained significant traction in field research. Nevertheless, neither mechanism behind neuron-selectivity nor cellular localization been determined. Here, we introduce NeuM, derivative NeuO, cell membranes. Furthermore, elucidate selective membrane trafficking that distinguishes neurons. In an aqueous buffer, NeuM autonomously assembles into micellar structures, leading quenching its fluorescence (Φ=0.001). Upon exposure neurons, micelles were internalized endosomes via clathrin-mediated endocytosis. Through endocytic recycling pathway, integrate membrane, dispersing fluorescent molecules (Φ=0.61). Molecular dynamics simulations demonstrated comparison possesses optimal lipophilicity molecular length, facilitating stable incorporation phospholipid layers. integration within allows prolonged monitoring well visualization structures.

Language: Английский

Citations

3
Fluorescent styrenes for mitochondrial imaging and viscosity sensing DOI

Deepmala Singh,

Mohini Ghorpade,

Ramprasad Regar

et al.

Photochemistry and Photobiology, Journal Year: 2024, Volume and Issue: 100(4), P. 936 - 945

Published: Feb. 22, 2024

Abstract Fluorophores bearing cationic pendants, such as the pyridinium group, tend to preferentially accumulate in mitochondria, whereas those with pentafluorophenyl groups display a distinct affinity for endoplasmic reticulum. In this study, we designed fluorophores incorporating and pendants examined their impact on sub‐cellular localization. Remarkably, exhibited notable propensity mitochondrial membrane. Furthermore, these revealed dual functionality by facilitating detection of viscosity changes within environment serving heavy‐atom‐free photosensitizers. With easy chemical tunability, wash‐free imaging, favorable signal‐to‐noise ratio, are valuable tools imaging mitochondria investigating cellular processes.

Language: Английский

Citations

0
Organelle‐Selective Probes DOI
Young‐Tae Chang,

Nam‐Young Kang

Published: June 2, 2023

Cells are isolated from environment through a phospholipid bilayer. Inside cell, there many small compartments segregated by membrane structure, so called organelles. The organelles include nucleus, nucleolus, ER, Golgi, mitochondria, lysosome, peroxisome and lipid droplets. free space in the cell is as cytosol. Selective staining of each organelle essential for dissection structure monitoring dynamic changes under various biological condition. design property selective probes discussed full details organelle. Above intracellular organelles, scope was expanded to extracellular vesicles nonmembrane bound condensates. behavior compared between live fixed cells, emphasizing challenges imaging. Finally, modeling methods were at end chapter.

Language: Английский

Citations

0
NeuM: A Neuon‐Selective Probe Incorporates into Live Neuronal Membranes via Enhanced Cathrin‐Mediated Endocytosis in Primary Neurons. DOI Creative Commons

Yoonsik Sung,

Lizaveta Gotina,

Kyu Hyeon Kim

et al.

Angewandte Chemie, Journal Year: 2023, Volume and Issue: 136(3)

Published: Dec. 8, 2023

Abstract The development of a small‐molecule probe designed to selectively target neurons would enhance the exploration intricate neuronal structures and functions. Among such probes, NeuO stands out as pioneer has gained significant traction in field research. Nevertheless, neither mechanism behind neuron‐selectivity nor cellular localization been determined. Here, we introduce NeuM, derivative NeuO, cell membranes. Furthermore, elucidate selective membrane trafficking that distinguishes neurons. In an aqueous buffer, NeuM autonomously assembles into micellar structures, leading quenching its fluorescence (Φ=0.001). Upon exposure neurons, micelles were internalized endosomes via clathrin‐mediated endocytosis. Through endocytic recycling pathway, integrate membrane, dispersing fluorescent molecules (Φ=0.61). Molecular dynamics simulations demonstrated comparison possesses optimal lipophilicity molecular length, facilitating stable incorporation phospholipid layers. integration within allows prolonged monitoring well visualization structures.

Language: Английский

Citations

0