The role of erythropoietin-loaded hydrogel versus adipose derived stem cell secretome in the regeneration of tongue defects DOI Creative Commons
Rana El-Qashty, Jilan M. Youssef, Eman Hany

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 18, 2024

Abstract Background Tongue defects have several etiologies and significantly affect the quality of life. This study was conducted to compare regenerative potential erythropoietin (EPO)-loaded hydrogel adipose derived stem cell (ADSC) secretome on tongue dorsum focusing role anti-inflammatory M2 macrophage phenotype. Methods Rats were subjected induction mechanical circular dorsal surface tongue, then divided into three groups; Group I (control): received 0.1 ml phosphate buffered saline, II (EPO): 5000 U/kg EPO-hydrogel, III (ADSC-Secretome): ADSC-Secretome. Treatments injected circumferentially around wound margins after induction. Seven fourteen days treatment, specimens obtained processed for histological immunohistochemical staining followed by relevant histomorphometric statistical analyses. Results groups presented with some epithelial regeneration at lateral margins, while center defect showed granulation tissue much inflammatory cells. The base muscle fibers new blood vessels, however group more enhanced neovascularization. Fourteen therapeutic intervention, completely covered epithelium showing a thin keratin layer regular rete pegs interdigitating underlying connective papillae, but papillae not restored. expressed better healing developing filiform papillae. vascularity well-arranged bundles. Both treated significant decrease in depth increase macrophages compared control both time intervals, there no difference between two groups. Conclusion treatments promising comparable results treatment reducing inflammation restoring architecture upregulation macrophage.

Language: Английский

Immunophenotyping of hemocytes from infected Galleria mellonella larvae as an innovative tool for immune profiling, infection studies and drug screening DOI Creative Commons
M. Gallorini, Beatrice Marinacci, Benedetta Pellegrini

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 8, 2024

Abstract In recent years, there has been a considerable increasing interest in the use of greater wax moth Galleria mellonella as an animal model. vivo pharmacological tests, concerning efficacy and toxicity novel compounds are typically performed mammalian models. However, latter is costly, laborious requires ethical approval. this context, G. larvae can be considered valid option due to their ease absence rules. Furthermore, it demonstrated that immune system these invertebrates similarity with one mammals, thus guaranteeing reliability model, mainly microbiological field. To better develop full potential we present approach characterize hemocyte population from highlight immuno modulation upon infection treatments. Our based on detection isolated hemocytes hemolymph cell membrane markers expressed by human cells inflammation infection, for instance CD14, CD44, CD80, CD163 CD200. This method highlights analogies between humans. provide innovative tool perform pre-clinical evaluations antimicrobial further proceed clinical trials support drug discovery campaigns.

Language: Английский

Citations

14
Macrophage polarization in bone implant repair: A review DOI Creative Commons

Zhengzheng Song,

Yuxi Cheng, Min-Min Chen

et al.

Tissue and Cell, Journal Year: 2023, Volume and Issue: 82, P. 102112 - 102112

Published: May 20, 2023

Macrophages (MΦ) are highly adaptable and functionally polarized cells that play a crucial role in various physiological pathological processes. Typically, MΦ differentiate into two distinct subsets: the proinflammatory (M1) anti-inflammatory (M2) phenotypes. Due to their potent immunomodulatory properties, have garnered significant attention recent decades. In context of bone implant repair, function is paramount importance. Depending on polarization phenotype, can exert varying effects osteogenesis, angiogenesis, inflammatory response around implant. This paper provides an overview repair implants.

Language: Английский

Citations

13
2-Substituted-4,7-dihydro-4-ethylpyrazolo[1,5-a]pyrimidin-7-ones alleviate LPS-induced inflammation by modulating cell metabolism via CD73 upon macrophage polarization DOI Creative Commons
Alessia Ricci, Susi Zara, Fabrizio Carta

et al.

Molecular Immunology, Journal Year: 2024, Volume and Issue: 170, P. 99 - 109

Published: April 20, 2024

Language: Английский

Citations

3
Sex differences in alcohol inhibits bone formation and promotes bone resorption in young male and female rats by altering intestinal flora, metabolites, and bone microenvironment DOI Creative Commons

Ming Cheng,

Hua Lu, Yiping Wu

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(5), P. e0323222 - e0323222

Published: May 8, 2025

Background Long-term alcohol intake has toxic effects on osteoblasts and osteoclasts, resulting in decreased bone density, which directly disrupts the composition of gut microbiota affects metabolism immune activity. The bones may be closely related to sex. This study investigated long-term consumption status different sexes by examining microbiota, metabolism, Methods Young male female rats were administered a Bio-Serv liquid diet containing 5% alcohol. capacity, morphology, formation, resorption, marrow activity, metabolite differences analyzed using hematoxylin eosin staining, micro-computed tomography, enzyme-linked immunosorbent assay, western blotting, 16S rRNA sequencing, untargeted metabolomics. Results Chronic resulted excessive osteoclast activation mineral density. Furthermore, reduced formation while increasing resorption. Bone loss was significantly more severe than rats, indicating that rat are also led polarization immunoreactivity toward M1 phenotype. In addition, chronic affected richness diversity intestinal ratio Firmicutes / Bacteroidetes . fecal metabolites, 754 differentially expressed metabolites identified. Conclusions increased inhibited young rats. Alcohol can affect metabolism. Female susceptible alcohol, possibly because have lower immunomodulatory

Language: Английский

Citations

0
Impact of Smoking on Macrophage‐Related Chemokines During Initial Peri‐Implantitis: A Prospective Cohort Study DOI Creative Commons
Buse Naz Buyukakcali Altay, Zeynep Turgut Cankaya, Mustafa Yılmaz

et al.

Clinical Implant Dentistry and Related Research, Journal Year: 2025, Volume and Issue: 27(3)

Published: May 21, 2025

ABSTRACT Objectives Smoking disrupts macrophage chemokine response and delays healing. This study aims to investigate the effect of smoking on peri‐implant crevicular fluid (PICF) levels macrophage‐related chemokines, C‐C motif ligand 2 (CCL‐2), 8 (CCL‐8), C‐X‐C 9 (CXCL‐9), 3 (CCL‐3), before after non‐surgical treatment initial peri‐implantitis. Methods Fifty‐five implants (27 non‐smoking [NSPI] 28 [SPI]) with peri‐implantitis (bleeding probing [BOP+], pocket depth [PPD] 6–7 mm) were included in study. Clinical parameters recorded, PICF samples collected 4 months treatment. concentrations CCL‐2, CCL‐8, CCL‐3, CXCL‐9 measured Luminex assay. The Mann–Whitney U ‐test, Wilcoxon signed‐rank test, repeated measures analysis variance test used analyze differences between within groups. Results Baseline CCL‐2 ( p < 0.001) = 0.026) (pg/30 s) significantly lower smokers compared non‐smokers, while no difference was observed for CCL‐3 two groups 0.320). Only decreased NSPI group 0.037). Conclusion disturbs expressions chemokines early phase These findings may indicate impaired control infection during explain accelerated progression disease smokers. not registered prior participant recruitment. Trial Registration https://clinicaltrials.gov/study/NCT06810401

Language: Английский

Citations

0
Modulating TRPV4 Channel Activity in Pro-Inflammatory Macrophages within the 3D Tissue Analog DOI Creative Commons
Parto Babaniamansour, Diego Jacho,

Skyler Niedzielski

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 230 - 230

Published: Jan. 19, 2024

Investigating macrophage plasticity emerges as a promising strategy for promoting tissue regeneration and can be exploited by regulating the transient receptor potential vanilloid 4 (TRPV4) channel. The TRPV4 channel responds to various stimuli including mechanical, chemical, selective pharmacological compounds. It is well documented that treating cells such epithelial fibroblasts with agonist enhances Ca2+ influx cells, which leads secretion of pro-inflammatory cytokines, while antagonist reduces both cytokine secretion. In this work, we investigated effect modulator compounds on U937-differentiated macrophages encapsulated within three-dimensional (3D) matrices. Despite offering more physiologically relevant model than 2D cultures, treatment 3D collagen matrices largely overlooked in literature. study, were treated an agonist, 500 nM GSK1016790A (TRPV4(+)), antagonist, 10 mM RN-1734 (TRPV4(−)), elucidate modulation at cellular extracellular levels. To evaluate phenotypic alterations following treatment, employed structural techniques (SEM, Masson’s trichrome, hybridizing peptide (CHP) staining), quantitative morphological measures assessment, genotypic methods real-time PCR (qRT-PCR) immunohistochemistry (IHC). Our data reveal alters cytoskeletal structure influences encapsulating them. Moreover, proved expression pro- anti-inflammatory genes, respectively, leading upregulation surface markers CD80 CD206. TRPV4(−) group, CD206 gene marker significantly upregulated 9- 2.5-fold, compared control group. These findings demonstrate utilized shift phenotype matrix toward desired state. This innovative approach addressing inflammation musculoskeletal tissues.

Language: Английский

Citations

2
Deciphering the Cell-Specific Effect of Osteoblast-Macrophage Crosstalk in Periodontitis DOI
Diego Jacho, Parto Babaniamansour, Raquel Osorio

et al.

Tissue Engineering Part A, Journal Year: 2023, Volume and Issue: 29(21-22), P. 579 - 593

Published: Aug. 28, 2023

In periodontitis, the bone remodeling process is disrupted by prevalent involvement of bacteria-induced proinflammatory macrophage cells and their interaction with osteoblast residing within infected tissue. The complex between needs to be deciphered understand dominant player in tipping balance from osteogenesis osteoclastogenesis. Yet, only a few studies have examined crosstalk osteoblasts macrophages using biomimetic three-dimensional (3D) tissue-like matrices. this study, we created cell-laden 3D tissue analog study indirect these two cell types direct synergistic effect when cultured on scaffold. cell-specific role osteoclast differentiation was investigated through osteoblast- macrophage-specific feedback studies. results suggested that were exposed osteoblasts-derived conditioned media mineralized matrix, M1 tended maintain phenotype. Further, secretions macrophages, they demonstrated elevated receptor activator nuclear factor-κB ligand (RANKL) expression decreased alkaline phosphate (ALP) activities compared osteogenic media. addition, upregulation tumor necrosis factor-alpha (TNF-α) c-Fos matrix indirectly increased RANKL reduced ALP activity osteoblasts, promoting contact coculturing markers (TNF-α interleukin-1β) expressions upregulated. contrast, anti-inflammatory (c-c motif chemokine 18 [CCL18]) downregulated, osteoclastogenic (TNF associated factor 6 [TRAF6] acid phosphatase 5, tartrate resistant [ACP5]) unchanged. data curbed while still preserved lineages. coculture did not express significantly different than collagen without macrophages. Contact has an anabolic bacteria-derived inflammatory environment.

Language: Английский

Citations

3
The role of erythropoietin-loaded hydrogel versus adipose derived stem cell secretome in the regeneration of tongue defects DOI Creative Commons
Rana El-Qashty, Jilan M. Youssef, Eman Hany

et al.

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 18, 2024

Abstract Background Tongue defects have several etiologies and significantly affect the quality of life. This study was conducted to compare regenerative potential erythropoietin (EPO)-loaded hydrogel adipose derived stem cell (ADSC) secretome on tongue dorsum focusing role anti-inflammatory M2 macrophage phenotype. Methods Rats were subjected induction mechanical circular dorsal surface tongue, then divided into three groups; Group I (control): received 0.1 ml phosphate buffered saline, II (EPO): 5000 U/kg EPO-hydrogel, III (ADSC-Secretome): ADSC-Secretome. Treatments injected circumferentially around wound margins after induction. Seven fourteen days treatment, specimens obtained processed for histological immunohistochemical staining followed by relevant histomorphometric statistical analyses. Results groups presented with some epithelial regeneration at lateral margins, while center defect showed granulation tissue much inflammatory cells. The base muscle fibers new blood vessels, however group more enhanced neovascularization. Fourteen therapeutic intervention, completely covered epithelium showing a thin keratin layer regular rete pegs interdigitating underlying connective papillae, but papillae not restored. expressed better healing developing filiform papillae. vascularity well-arranged bundles. Both treated significant decrease in depth increase macrophages compared control both time intervals, there no difference between two groups. Conclusion treatments promising comparable results treatment reducing inflammation restoring architecture upregulation macrophage.

Language: Английский

Citations

0