A Review on Bioengineering the Bovine Mammary Gland: The Role of the Extracellular Matrix and Reconstruction Prospects

Bioengineering, Journal Year: 2025, Volume and Issue: 12(5), P. 501 - 501

Published: May 9, 2025

The mammary gland is a modified sweat responsible for milk production. It affected by diseases that reduce animals’ quality of life, consequently leading to economic losses in livestock. With advancements tissue bioengineering and regenerative medicine, studying the extracellular matrix (ECM) bovine can improve our understanding its physiology processes affect it. This knowledge could also enable development sustainable therapeutic alternatives both dairy production chain human oncology research. A common approach medicine decellularization, process removes all cells from while preserving architecture ECM components subsequent recellularization. success recellularization depends on obtaining immunologically compatible scaffolds using appropriate cell culture sources methods ensure functionality. However, technology still faces challenges due specific requirements high costs. Here, we review literature biomaterials engineering, providing an overview advances bioengineering, with focus species. methodology employed consists structured search scientific databases, including PubMed, Google Scholar, SciELO, keywords related engineering gland. selection criteria prioritized peer-reviewed articles published between 2002 2025 demonstrated relevance contributed bioengineering. Although research this topic has advanced, vascularization, maturation, scalability remain key barriers widespread application viability.

Language: Английский

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Cryomedical technologies as a key to effective decellularization in the creation of scaffolds for vascular transplantation

The Journal of V N Karazin Kharkiv National University series Medicine, Journal Year: 2024, Volume and Issue: 50, P. 366 - 386

Published: Nov. 13, 2024

Background. Cardiovascular diseases (CVDs) rank among the top three causes of death worldwide. In Europe, 3.9 million deaths annually are attributed to CVDs, with 1.8 occurring citizens European Union (EU) countries. The total cost treating patients CVDs in EU countries amounts €210 billion per year. Currently, primary treatment strategy for advanced stages CVD remains bypass surgery. A significant increase demand vascular grafts over past decade, particularly small-caliber vessels cardiovascular procedures, combined a shortage donor and limitations artificial prostheses, makes tissue engineering high-demand field. Purpose – characterize modern approaches creating decellularized scaffolds based on data from open sources. Materials Methods. selection publications was conducted using databases such as PubMed, Clinical Key Elsevier, Cochrane Library, eBook Business Collection, others, focusing contemporary methods scaffolds. first stage involved searching literature sources Keywords: decellularization, extracellular matrix, scaffold. second stage, article abstracts were reviewed, not meeting study criteria excluded. third examining full texts selected articles compliance inclusion research relevance. Inclusion subjected content analysis included: 1) coverage current information creation scaffolds; 2) alignment studies key principles evidence-based medicine; 3) access full-text article. Results. attempt create blood vessel substitute made by Weinberg C.B. & Bell E. 1986. Biological scaffolds, composed matrix (ECM), commonly used various reconstructive surgical procedures increasingly employed regenerative medicine strategies organ replacement. ECM is complex network macromolecules that provides an appropriate local microenvironment cell survival activity vivo, influencing shape, metabolism, function, migration, proliferation, differentiation. scaffold, turn, can be defined three-dimensional platform necessary actions ranging cell-biomaterial interaction adhesion controlled biodegradation rates correspond regeneration. Decellularization method removing cellular components organs or tissues acellular scaffold capable providing biomimetic microenvironment. physicochemical signals biological efficacy maintained after thus substrate mechanical support 3D carrier subsequent recellularization. complexity duration decellularization protocols generally proportional degree geometric preservation desired processing. Conclusions. rising incidence need intervention have underscored necessity, other things, substitutes vessels, especially those under 6 mm diameter. Various sources, including human animal cadavers, been identified native decellularization. pattern new trend actively pursued, directly offering spatial control angiogenesis, closely mimicking natural environment. Cryopreservation one most common graft storage. freezing-thawing process effectively lyses cells promising development

Language: Английский

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Standardizing decellularization protocols for optimized uterine tissue bioengineering

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 28, P. 183 - 190

Published: Dec. 21, 2024

Language: Английский

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