Enhancing the wound healing process through local injection of exosomes derived from blood serum: An in vitro and in vivo assessment DOI Creative Commons
Mehdi Rasti,

Amir Hossein Parniaei,

Leila Dehghani

et al.

Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 281 - 289

Published: June 1, 2024

The skin plays a crucial role as protective barrier against external factors, but disruptions to its integrity can lead wound formation and hinder the natural healing process. Scar delayed present significant challenges in injury treatment. While alternative approaches such substitutes tissue engineering exist, they are often limited accessibility cost. Exosomes have emerged potential solution for due their regenerative properties. In this study, exosomes were isolated from human blood serum using kit. characterized, effects on cell migration assessed vitro. Additionally, capacity of was evaluated vivo rat full-thickness model. Our vitro findings revealed that significantly promoted migration. experiments demonstrated injection at different areas accelerated process, resulting closure, collagen synthesis, vessel formation, angiogenesis area. These results suggest promising therapeutic expediting minimizing scar formation. study highlight novel approach enhancing healing. showed positive both closure studies, suggesting use therapy injuries. Further research is needed fully understand mechanisms underlying beneficial optimize application clinical settings.

Language: Английский

Exosomes in skin photoaging: biological functions and therapeutic opportunity DOI Creative Commons

Amirhossein Hajialiasgary Najafabadi,

Mohammad Hasan Soheilifar, Nastaran Masoudi-Khoram

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 12, 2024

Abstract Exosomes are tiny extracellular vesicles secreted by most cell types, which filled with proteins, lipids, and nucleic acids (non-coding RNAs, mRNA, DNA), can be released donor cells to subsequently modulate the function of recipient cells. Skin photoaging is premature aging skin structures over time due repeated exposure ultraviolet (UV) evidenced dyspigmentation, telangiectasias, roughness, rhytides, elastosis, precancerous changes. associated aging-related processes including, oxidative stress, inflammation, senescence. Anti-aging features exosomes have been implicated in various vitro pre-clinical studies. Stem cell-derived restore physiological regenerate or rejuvenate damaged tissue through mechanisms such as decreased expression matrix metalloproteinase (MMP), increased collagen elastin production, modulation intracellular signaling pathways well as, intercellular communication. All these evidences promising for therapeutic potential photoaging. This review aims investigate molecular effects

Language: Английский

Citations

25
Extracellular Vesicles and Hydrogels: An Innovative Approach to Tissue Regeneration DOI Creative Commons
Amir Hashemi,

Masoumeh Ezati,

Minoo Partovi Nasr

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(6), P. 6184 - 6218

Published: Jan. 31, 2024

Extracellular vesicles have emerged as promising tools in regenerative medicine due to their inherent ability facilitate intercellular communication and modulate cellular functions. These nanosized transport bioactive molecules, such proteins, lipids, nucleic acids, which can affect the behavior of recipient cells promote tissue regeneration. However, therapeutic application these is frequently constrained by rapid clearance from body inability maintain a sustained presence at injury site. In order overcome obstacles, hydrogels been used extracellular vesicle delivery vehicles, providing localized controlled release system that improves efficacy. This Review will examine role vesicle-loaded regeneration, discussing potential applications, current challenges, future directions. We investigate origins, composition, characterization techniques vesicles, focusing on recent advances exosome profiling machine learning this field. addition, we properties make them ideal carriers. Recent studies utilizing combination for regeneration be highlighted, comprehensive overview research landscape

Language: Английский

Citations

12
Intradermal Injection of Hybrid Complexes of High- and Low-Molecular-Weight Hyaluronan: Where Do We Stand and Where Are We Headed in Regenerative Medicine? DOI Open Access
Dalvi Humzah,

Beatriz Molina,

Giovanni Salti

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3216 - 3216

Published: March 12, 2024

Hyaluronic acid (HA) is a remarkably multifaceted biomacromolecule, playing role in regulating myriad biological processes such as wound healing, tissue regeneration, anti-inflammation, and immunomodulation. Crosslinked high- low-molecular-weight hyaluronic hydrogels achieve higher molar concentrations, display slower degradation, allow optimal product diffusion, while harnessing the synergistic contribution of different-molecular-weight hyaluronans. A recent innovation world synthesis represented by NAHYCO® Hybrid Technology, thermal process leading to hybrid cooperative complexes (HCC). This review summarizes current literature on vitro studies vivo applications HCC, from facial body rejuvenation future perspectives skin dermatology, genitourinary pathologies.

Language: Английский

Citations

8
EXOSOMES FOR SKIN TREATMENT: THERAPEUTIC AND COSMETIC APPLICATIONS DOI Creative Commons

Harsha Sreeraj,

R. AnuKiruthika,

K. S. Tamilselvi

et al.

Nano TransMed, Journal Year: 2024, Volume and Issue: unknown, P. 100048 - 100048

Published: Sept. 1, 2024

Language: Английский

Citations

8
Lysyl oxidase like-1 deficiency in optic nerve head astrocytes elicits reactive astrocytosis and alters functional effects of astrocyte derived exosomes DOI
Harsh N. Hariani, Anita K. Ghosh,

Sasha Rosen

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 240, P. 109813 - 109813

Published: Feb. 6, 2024

Language: Английский

Citations

5
The Innovative and Evolving Landscape of Topical Exosome and Peptide Therapies: A Systematic Review of the Available Literature DOI Creative Commons

Makenna Ash,

Meira Zibitt,

Orr Shauly

et al.

Aesthetic Surgery Journal Open Forum, Journal Year: 2024, Volume and Issue: 6

Published: Jan. 1, 2024

Topical antiaging therapies provide noninvasive delivery of active therapeutics. Exosomes, or extracellular nanovesicles, and peptides, small strings amino acids, have shown promise as topical in early trials, but neither is FDA approved. This review aims to elucidate the current future landscape exosomes peptides therapeutics for skin rejuvenation. A literature search was conducted using keywords "peptides" OR "exosomes" AND "skin" "rejuvenation." Primary endpoints included mechanisms action humans live animals well clinical data supporting use topically rejuvenation wound healing. Secondary were safety, side effects, efficacy. The articles collected, organized, sorted Covidence software (Melbourne, Australia) systematic review. Nine evaluating application 9 met inclusion criteria. found increase collagen deposition, accelerate healing, improve overall cosmesis. Several trials are currently underway. appearance fine lines wrinkles, elasticity viscoelasticity, texture, thickness, potential accelerated Peptides quite common "cosmeceutical" products, several patents been filed peptide products aimed at increasing could indicate a movement toward pursuing approval. exosome purpose appears promising. Preliminary from studies reviewed here indicates that these be safe effective.

Language: Английский

Citations

4
Use of Exosomes for Cosmetics Applications DOI Creative Commons
Luis Jesús Villarreal-Gómez,

Sergio Origel-Lucio,

Daniela Alejandra Hernández-Hernández

et al.

Cosmetics, Journal Year: 2025, Volume and Issue: 12(1), P. 9 - 9

Published: Jan. 13, 2025

This study addresses a significant gap in the clinical validation and standardization of exosome-based applications within domains cosmetics regenerative medicine. Despite offering thorough overview potential therapeutic benefits underlying mechanisms exosomes, manuscript underscores several unresolved challenges, including paucity evidence, regulatory barriers, inconsistencies standardization, incomplete mechanistic understanding. The primary aim this research is to conduct systematic comprehensive review existing studies on exosomes skincare. To achieve this, literature was performed, drawing major medical database PubMed. approach facilitated collection meta-analysis relevant data, ensuring rigorous assessment recent advancements exosome research. main outcomes highlight multifaceted roles promoting skin rejuvenation mitigating signs aging. Specific discussed include pigmentation correction, wound healing, tissue repair, innovative delivery mechanisms. Moreover, examines emerging plastic surgery Challenges limitations, such as constraints, hurdles, need for further validation, are critically analyzed, providing framework future directions.

Language: Английский

Citations

0
Needle Radiofrequency Combined with Topical Exosome Therapy for Moderate to Severe Acne DOI Creative Commons
Jovian Wan,

Song Eun Yoon,

Sky Wong

et al.

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 141 - 141

Published: Jan. 21, 2025

Objective: This case series aims to evaluate the efficacy, patient satisfaction, and safety of combined needle radiofrequency (RF) topical exosome therapy for moderate severe acne. Methods: study involved 22 patients (12 females 10 males, ages 18–35) with acne who underwent RF (Xomage, Zishel Bio Inc., Seoul, Republic Korea) treatments. Each completed between 6 sessions, conducted weekly over three-week intervals. Acne severity was assessed using Investigator’s Global Assessment (IGA) scale, while satisfaction measured on a 5-point Likert scale. Clinical photographs were taken at baseline after final treatment session. Results: All showed improvement in mean decrease IGA score 2.5 points from assessment. Patient high, majority expressing skin texture reduction. Conclusion: Needle appears be an effective reducing lesions improving quality, demonstrating strong profile high satisfaction.

Language: Английский

Citations

0
Exosomes in Dermatological Research: Unveiling Their Multifaceted Role in Cellular Communication, Healing, and Disease Modulation DOI Creative Commons
Daniela Pinto,

Giammaria Giuliani,

Giorgia Mondadori

et al.

Cosmetics, Journal Year: 2025, Volume and Issue: 12(1), P. 16 - 16

Published: Jan. 22, 2025

Exosomes, a subtype of extracellular vehicles (EVs), play pivotal role in cellular communication and have gained considerable attention dermatological research. Formed through the inward budding endosomal membrane, exosomes facilitate transfer proteins, lipids, nucleic acids, including microRNAs (miRNAs), thereby influencing behavior function recipient cells. These vesicles are secreted by various cell types, keratinocytes, crucial for maintaining skin homeostasis, regulating immune responses, promoting wound healing. Exosomes demonstrated therapeutic potential addressing conditions such as hair disorders, cancers photoaging enhanced regeneration reduced oxidative stress. However, they also implicated disease progression, with pathogens utilizing exosome release to evade host responses. Recent studies highlight diverse origins functions exosomes, suggesting their promise innovative agents dermatology. As research continues elucidate multifaceted roles, represent frontier understanding intercellular developing novel treatments skin-related diseases, underscoring impact on both health clinical applications. This review synthesizes existing literature biology isolation focus implications contexts.

Language: Английский

Citations

0
Stimulatory Effect of Mammalian Exosomes on Intradermal Fibroblast Proliferation DOI Creative Commons
Alina Kilasoniya, Lidia Zabegina, Andrey Shalaev

et al.

Journal of Cosmetic Dermatology, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 1, 2025

ABSTRACT Background This study investigates the potential of extracellular vesicles (EVs) in skin regeneration and rejuvenation. EVs, nanoscale released by various cell types, play a crucial role intercellular communication. Objective To reaffirm pivotal blood‐derived exosomes communication their for tissue regeneration, leveraging existing research, including human data, to advocate as viable cell‐free therapy health. Methods The employs novel isolation technique combining PEG Dextran with ultracentrifugation extract EVs from plasma. Characterization techniques, NTA, AFM, Cryo‐TEM, FC, confirm successful characterization EVs. Results demonstrates positive effects on fibroblast proliferation, collagen, elastin production murine models. Despite advancements, challenges persist obtaining consistent quality concentration. findings support clinical relevance health suggest applications Future research directions limitations are also discussed, contributing evolving understanding EVs‐based therapies.

Language: Английский

Citations

0