Effect of Adding Gold Nanoparticles on the Anti-Candidal Activity and Release Profile of Itraconazole from Hydrogels DOI Creative Commons
Radosław Balwierz, Paweł Biernat, Dawid Bursy

et al.

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(23), P. 11125 - 11125

Published: Nov. 29, 2024

Gold nanoparticles have been identified as a promising avenue for the development of drug carriers, particularly in context antimicrobial delivery, where limited solubility represents significant challenge. The ability gold to penetrate biofilms and disrupt fungal cell membranes makes them an effective tool support antifungal therapy, especially against resistant strains. also demonstrate synergistic effects with chemotherapeutics can influence release profile active substances. This study aimed develop topical hydrogel formulation containing itraconazole (ITZ), addition nanoparticles, enhance its therapeutic properties. Due ITZ’s poor water solubility, three types (AuNPs) different sizes were synthesized subsequently coated itraconazole. resulting formulations incorporated into carbopol gels their diffuse through semipermeable was assessed. findings demonstrated that combination elevated diffusion coefficient twice level observed without nanoparticles. Furthermore, combined effect reference Candida albicans strain investigated. growth-inhibitory on this strain, indicating could potentially be employed treatment infections. confirms hydrogels obtained, offering enhanced diffusion.

Language: Английский

Diagnosis of invasive fungal infections: challenges and recent developments DOI Creative Commons
Wenjie Fang, Junqi Wu,

Mingrong Cheng

et al.

Journal of Biomedical Science, Journal Year: 2023, Volume and Issue: 30(1)

Published: June 19, 2023

The global burden of invasive fungal infections (IFIs) has shown an upsurge in recent years due to the higher load immunocompromised patients suffering from various diseases. role early and accurate diagnosis aggressive containment infection at initial stages becomes crucial thus, preventing development a life-threatening situation. With changing demands clinical mycology, field diagnostics evolved come long way traditional methods microscopy culturing more advanced non-culture-based tools. advent powerful approaches such as novel PCR assays, T2 Candida, microfluidic chip technology, next generation sequencing, new biosensors, nanotechnology-based tools, artificial intelligence-based models, face is constantly for better. All these advances have been reviewed here giving latest update our readers most orderly flow.A detailed literature survey was conducted by team followed data collection, pertinent extraction, in-depth analysis, composing sub-sections final review. review unique its kind it discusses molecular methods; serology-based biosensor technology; machine learning-based all under one roof. To best knowledge, there no covering fields (especially technology learning using intelligence) with relevance infections.The will undoubtedly assist updating scientific community's understanding advancements that are on horizon may be implemented adjuncts diagnostic algorithms.

Language: Английский

Citations

155
Synergistic Antibacterial Activity of Green Synthesized Silver Nanomaterials with Colistin Antibiotic against Multidrug-Resistant Bacterial Pathogens DOI Creative Commons
Mohamed Taha Yassin, Ashraf Abdel‐Fattah Mostafa, Abdulaziz A. Al‐Askar

et al.

Crystals, Journal Year: 2022, Volume and Issue: 12(8), P. 1057 - 1057

Published: July 29, 2022

The high frequency of nosocomial bacterial infections caused by multidrug-resistant pathogens contributes to significant morbidity and mortality worldwide. As a result, finding effective antibacterial agents is critical importance. Hence, the aim present study was greenly synthesize silver nanoparticles (AgNPs) utilizing Salvia officinalis aqueous leaf extract. biogenic AgNPs were characterized different physicochemical techniques such as energy-dispersive X-ray spectroscopy (EDX), ultraviolet-visible spectrophotometry (UV-Vis), diffraction analysis (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) analysis. Additionally, synergistic antimicrobial effectiveness biosynthesized with colistin antibiotic against strains evaluated standard disk diffusion assay. bioformulated revealed features, small particle size 17.615 ± 1.24 nm net zeta potential value −16.2 mV. elemental mapping that main element, recording relative mass percent 83.16%, followed carbon (9.51%), oxygen (5.80%), silicon (0.87%), chloride (0.67%). disc assay showed potency tested pathogens, highest efficiency Escherichia coli strain an inhibitory zone diameter 37.86 0.21 mm at concentration 100 µg/disk. In addition, activity significantly higher than (p ≤ 0.05) multidrug resistant namely, Acinetobacter baumannii. antibiotic, demonstrating A. baumannii (85.57%) Enterobacter cloacae (53.63%), E. (35.76%), Klebsiella pneumoniae (35.19%), Salmonella typhimurium (33.06%), Pseudomonas aeruginosa (13.75%). conclusion, unique characteristics activities pathogens. Consequently, potent effect AgNPs–colistin combination highlights this for fabrication highly coatings in intensive care units successful control spread infections.

Language: Английский

Citations

39
Green Biofabrication of Silver Nanoparticles of Potential Synergistic Activity with Antibacterial and Antifungal Agents against Some Nosocomial Pathogens DOI Creative Commons
Fatimah Al-Otibi, Mohamed Taha Yassin, Abdulaziz A. Al‐Askar

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(4), P. 945 - 945

Published: April 4, 2023

Nosocomial bacterial and fungal infections are one of the main causes high morbidity mortality worldwide, owing to prevalence multidrug-resistant microbial strains. Hence, study aims synthesize, characterize, investigate antifungal antibacterial activity silver nanoparticles (AgNPs) fabricated using Camellia sinensis leaves against nosocomial pathogens. The biogenic AgNPs revealed a small particle diameter 35.761 ± 3.18 nm based on transmission electron microscope (TEM) graphs negative surface charge -14.1 mV, revealing repulsive forces between nanoparticles, which in turn indicated their colloidal stability. disk diffusion assay confirmed that Escherichia coli was most susceptible strain (200 g/disk), while lowest sensitive found be Acinetobacter baumannii with relative inhibition zones 36.14 0.67 21.04 0.19 mm, respectively. On other hand, µg/disk) exposed efficacy Candida albicans zone 18.16 0.14 mm diameter. synergistic both tigecycline clotrimazole A. C. albicans, In conclusion, demonstrated distinct physicochemical properties potential bioactivity tigecycline, linezolid, gram-negative, gram-positive, strains, This is paving way for development effective antimicrobial combinations management pathogens intensive care units (ICUs) health settings.

Language: Английский

Citations

29
Biogenic silver nanoparticles as antifungal agents DOI Creative Commons
Javier Mussin, Gustavo Giusiano

Frontiers in Chemistry, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 6, 2022

In recent years, an increase in multidrug-resistant fungal strains has been observed, which, together with the limited number of clinically available antifungal agents, highlights need for development new agents. Due to proven activity silver nanoparticles (AgNPs), there is a growing interest their use treatment infections. Nanoparticles are usually synthesised through variety physical and chemical processes that costly pollute environment. For this reason, biogenic synthesis emerging as environmentally friendly technology strategies increasingly based on AgNPs agents clinical use. The aim review compare different summarise current knowledge mechanisms action resistance fungi AgNPs. Finally, general analysis toxicity human veterinary medicine performed.

Language: Английский

Citations

38
Green Synthesis of Zinc Oxide Nanocrystals Utilizing Origanum majorana Leaf Extract and Their Synergistic Patterns with Colistin against Multidrug-Resistant Bacterial Strains DOI Creative Commons
Mohamed Taha Yassin, Abdulaziz A. Al‐Askar,

Khalid Maniah

et al.

Crystals, Journal Year: 2022, Volume and Issue: 12(11), P. 1513 - 1513

Published: Oct. 25, 2022

There is a crucial necessity for the formulation of efficient antimicrobial agents owing to increasing prevalence hospital-acquired bacterial infections triggered by multidrug-resistant microbes that result in significant deaths and illnesses around world. Hence, current investigation examined antibacterial proficiency zinc oxide nanoparticles formulated utilizing green route against strains were resistant multiple drugs. In addition, synergistic action ZnO (ZnO NPs) combined with colistin was investigated tested microbial determine efficiency bioinspired boosting antibiotic. Incidentally, synthesized using water extract Origanum majorana leaves these nanomaterials physicochemically characterized different analytical techniques. The bioactivity appraised agar diffusion method. biogenic NPs at concentration 100 μg/disk revealed compelling efficacy strains, expressing maximum Escherichia coli strain clear zone diameter 38.16 ± 0.18 mm. remarkable might be accredited tiny particle size bioformulated 12.467 1.36 nm. net charge −14.8 mV while XRD analysis confirmed their hexagonal wurtzite structure. Furthermore, showed promising potency demonstrating respective synergism proportions 91.05, 79.07, 75.04, 75.25, 56.28 10.60% E. coli, Klebsiella pneumoniae, Acinetobacter baumannii, Salmonella typhimurium, Enterobacter cloacae, Pseudomonas aeruginosa, respectively. conclusion, O. mediated unique physicochemical characteristics effective drug-resistant strains. These could used synthesis coatings control hospital acquired caused pathogens.

Language: Английский

Citations

29
Synergistic antibacterial activity of biogenic AgNPs with antibiotics against multidrug resistant bacterial strains DOI Creative Commons

Khalid Maniah,

Fatimah Olyan Al-Otibi,

Sara Mohamed

et al.

Journal of King Saud University - Science, Journal Year: 2024, Volume and Issue: 36(10), P. 103461 - 103461

Published: Sept. 21, 2024

Language: Английский

Citations

7
Nanotechnology-Based Strategies to Combat Multidrug-Resistant Candida auris Infections DOI Creative Commons
‏Helal F. Hetta, Yasmin N. Ramadan, Israa M.S. Al-Kadmy

et al.

Pathogens, Journal Year: 2023, Volume and Issue: 12(8), P. 1033 - 1033

Published: Aug. 13, 2023

An emerging multidrug-resistant pathogenic yeast called Candida auris has a high potential to spread quickly among hospitalized patients and immunodeficient causing nosocomial outbreaks. It the cause pandemic outbreaks in about 45 nations with mortality rates. Additionally, fungus become resistant decontamination techniques can survive for weeks hospital environment. Nanoparticles might be good substitute treat illnesses brought on by this newly discovered pathogen. have trend hot topic recent years combat fatal fungus. This review gives general insight into epidemiology of C. infection. discusses current conventional therapy mechanism resistance development. Furthermore, it focuses nanoparticles, their different types, up-to-date trials evaluate promising efficacy nanoparticles respect auris.

Language: Английский

Citations

11
Antibacterial efficacy of silver nanoparticles (AgNPs) against metallo-β-lactamase and extended spectrum β-lactamase producing clinically procured isolates of Pseudomonas aeruginosa DOI Creative Commons

Maria Muddassir,

Almas Raza,

Sadaf Munir

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Nov. 30, 2022

Resistance to carbapenems is a global threat, especially in developing countries with limited health resources. Prevalence, antibiogram, PCR detection of antibiotic resistance genes, and potency Silver Nanoparticles (AgNPs) against multidrug-resistant (MDR) Pseudomonas aeruginosa were studied. Kirby-Bauer disc method used study antibiogram drug genes respectively 255 isolates obtained from tertiary care hospital. nitrate (AgNO3) precursor salts reacted Aspergillus flavus culture filtrate trigger the extracellular mycosynthesis AgNPs. Mycosynthesis was first monitored regularly by visible ultraviolet spectroscopy that recorded AgNP peaks approximately 400-470 nm. Confirmation Transmission electron micrographs provided confirmation AgNPs formed within range 5-30 Individual combined antibacterial activity ten antibiotics analyzed. Pearson correlation coefficients (r) calculated for phenotypic genotypic multidrug resistance. Data evaluated using SPSS version 20. p-value < 0.05 considered statistically significant. 61.5% carbapenemase producers (p 0.01). The frequency blaIMP-1, blaSHV, blaVIM, blaOXA, blaTEM 13%, 32%, 15%, 21%, 43%, respectively. reducing order antimicrobial piperacillin/tazobactam + (31 mm), cefoxitin (30 mm) > amikacin (25 aztreonam (23 meropenem (22 imipenem (20 gentamycin (17 ciprofloxacin (16 cefoperazone/sulbactam (14 ≥ ceftazidime mm). conjugated effect plus showed 0.15-3.51 (average 2.09) fold-area augmentation activity. effectively inhibited MDR aeruginosa. To best our understanding, this an inaugural report Punjab Pakistan enlisting co-expression Metallo-β-lactamases, extended-spectrum β-lactamases, AmpC-β-lactamase antibiotic-AgNPs.

Language: Английский

Citations

18
Antifungal effects of thiosemicarbazone-chalcones on Aspergillus, Candida and Sporothrix strains DOI
William Queiroz Felippe, Igor Resendes Barbosa,

A. A. P. Oliveira

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(1)

Published: Jan. 1, 2025

Language: Английский

Citations

0
Silver Nanoparticles: A Versatile Tool Against Infectious and Non-Infectious Diseases DOI Creative Commons
Sara González-Fernández, Noelia Blanco-Agudín, David Rodrı́guez

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 289 - 289

Published: March 11, 2025

Silver nanoparticles possess remarkable properties that render them highly beneficial for medical applications in both infectious and non-infectious diseases. Among their most renowned attributes is antimicrobial activity. They have demonstrated efficacy against a wide range of bacteria, fungi, protozoa, viruses. Additionally, the antitumor anti-diabetic silver nanoparticles, along with ability to promote wound healing application as biosensors, underscore therapeutic potential various conditions. As are employed purposes, toxicity must be considered. While present promising alternative domain, further research needed elucidate precise mechanisms action, optimize efficacy, mitigate any health risks associated use.

Language: Английский

Citations

0