Targeted Elimination of Influenza Virus and Infected Cells with Near-Infrared Antiviral Photoimmunotherapy (NIR-AVPIT)

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(2), P. 173 - 173

Published: Jan. 28, 2025

Background: Seasonal influenza causes significant morbidity and mortality each year. Since viruses can easily acquire drug-resistant mutations, it is necessary to develop new antiviral strategies with different targets. Near-infrared photoimmunotherapy (NIR-PIT) a type of anti-cancer therapy that has recently attracted considerable attention, favorable outcomes reported for several cancers. In this study, we investigated whether approach could be used as novel anti-influenza destroy virus infected cells. Methods: To evaluate the efficacy near-infrared (NIR-AVPIT), prepared an anti-hemagglutinin (HA) monoclonal antibody without neutralizing activity against A (FluV) labeled IR-700 reacted FluV cells, well HA-expressing HEK293 Results: NIR-AVPIT strongly inactivated virions, suppressed cytopathic effects, achieved more than 4-log reduction in viral RNA amplification. Treatment FluV-infected cells antibody-IR700 complex NIR early stages infection significantly inhibited propagation, double treatment time apart exerted greater inhibitory effect. rapidly induced morphological changes proliferation these Conclusions: These results suggest targeting HA antigens inactivate eliminate vitro. This strategy promising treat various infections, including influenza.

Language: Английский

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Diagnostic utility of rapid antigen testing as point-of-care test for influenza and other respiratory viruses in patients with acute respiratory illness

Diagnostic Microbiology and Infectious Disease, Journal Year: 2024, Volume and Issue: 111(2), P. 116600 - 116600

Published: Nov. 5, 2024

Language: Английский

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Comparative Clinical Evaluation of a Novel FluA/FluB/SARS-CoV-2 Multiplex LAMP and Commercial FluA/FluB/SARS-CoV-2/RSV RT-qPCR Assays

Diagnostics, Journal Year: 2023, Volume and Issue: 13(8), P. 1432 - 1432

Published: April 16, 2023

Influenza and coronaviruses cause highly contagious respiratory diseases that millions of deaths worldwide. Public health measures implemented during the current coronavirus disease (COVID-19) pandemic have gradually reduced influenza circulation As COVID-19 relaxed, it is necessary to monitor control seasonal this pandemic. In particular, development rapid accurate diagnostic methods for paramount importance because both significant public economic impacts. To address this, we developed a multi-loop-mediated isothermal amplification (LAMP) kit capable simultaneously detecting A/B SARS-CoV-2. The was optimized by testing various ratios primer sets (FluA/FluB) SARS-CoV-2 internal (IC). FluA/FluB/SARS-CoV-2 multiplex LAMP assay showed 100% specificity uninfected clinical samples sensitivities 90.6%, 86.89%, 98.96% kits against A, B, samples, respectively. Finally, attribute agreement analysis tests indicated substantial between FluA/FluB/SARS-CoV-2/IC commercial AllplexTM SARS-CoV-2/FluA/FluB/RSV assays.

Language: Английский

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Development of a rapid, sensitive detection method for SARS‐CoV‐2 and influenza virus based on recombinase polymerase amplification combined with CRISPR‐Cas12a assay

Journal of Medical Virology, Journal Year: 2023, Volume and Issue: 95(11)

Published: Nov. 1, 2023

Abstract Respiratory tract infections are associated with the most common diseases transmitted among people and remain a huge threat to global public health. Rapid sensitive diagnosis of causative agents is critical for timely treatment disease control. Here, we developed novel method based on recombinase polymerase amplification (RPA) combined CRISPR‐Cas12a detect three viral pathogens, including SARS‐CoV‐2, influenza A, B, which cause similar symptom complexes flu cold in respiratory tract. The detection can be completed within 1 h, faster than other standard methods, limit approximately 10 2 copies/μL. Additionally, this system highly specific there no cross‐reactivity pathogens. Based assay, further more simplified RPA/CRISPR‐Cas12a lateral flow assay manual microfluidic chip, simultaneously these viruses. This low‐cost rapid sensitive, could applied field resource‐limited areas without bulky expensive instruments, providing powerful tools point‐of‐care diagnostic.

Language: Английский

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Performance evaluation of the Panbio COVID-19/Flu A&B Panel for detection of SARS-CoV-2, influenza A, and influenza B antigens using mid-turbinate nasal swabs

Journal of Clinical Microbiology, Journal Year: 2024, Volume and Issue: 62(7)

Published: June 18, 2024

The Panbio COVID-19/Flu A&B Panel (Abbott) is an

Language: Английский

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The convergent evolution of influenza A virus: Implications, therapeutic strategies and what we need to know

Current Research in Microbial Sciences, Journal Year: 2023, Volume and Issue: 5, P. 100202 - 100202

Published: Jan. 1, 2023

Influenza virus infection, more commonly known as the 'cold flu', is an etiological agent that gives rise to recurrent annual flu and many pandemics. Dated back 1918- Spanish Flu, influenza infection has caused loss of human lives significantly impacted economy daily lives. can be classified into four different genera: A-D, with former two, A B, relevant humans. The capacity antigenic drift shift in given novel variants, rendering vaccines antiviral therapies useless. In light emergence a betacoronavirus, SARS-CoV-2, unravelling underpinning mechanisms support epidemics pandemics essential. Given symptom similarities between covid it crucial reiterate what we know about infection. This review aims describe origin evolution Apart from that, risk factors entail implication co-infections, especially regarding COVID-19 pandemic further discussed. addition, strategies, including potential drug repositioning, are discussed this context. diagnostic approach also critically effort understand better prepare for upcoming variants future. Lastly, encapsulates challenges curbing spread provides insights future directions management.

Language: Английский

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Evaluation of Multiplex Rapid Antigen Tests for the Simultaneous Detection of SARS-CoV-2 and Influenza A/B Viruses

Biomedicines, Journal Year: 2023, Volume and Issue: 11(12), P. 3267 - 3267

Published: Dec. 9, 2023

Single-target rapid antigen tests (RATs) are commonly used to detect highly transmissible respiratory viruses (RVs), such as severe acute syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses. The simultaneous detection of RVs presenting overlapping symptoms is vital in making appropriate decisions about treatment, isolation, resource utilization; however, few studies have evaluated multiplex RATs for SARS-CoV-2 other RVs. We assessed the diagnostic performance targeting both A/B with GenBody Influenza/COVID-19 Ag Triple, InstaView COVID-19/Flu Combo (InstaView), STANDARDTM Q COVID-19 Test, Influenza Test kits using 974 nasopharyngeal swab samples. cycle threshold values obtained from real-time reverse transcription polymerase chain reaction results showed higher sensitivity (72.7-100%) when were below, rather than above, cut-off values. kit exhibited significantly positivity rates (80.21% SARS-CoV-2, 61.75% A, 46.15% B) (25.57 21.19 22.35 two kits, was able Omicron subvariants. Therefore, best choice routine screening local communities.

Language: Английский

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Simultaneous detection of influenza A, B and respiratory syncytial virus in wastewater samples by one-step multiplex RT-ddPCR assay

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: May 20, 2024

Abstract Background After the occurrence of COVID-19 pandemic, detection other disseminated respiratory viruses using highly sensitive molecular methods was declared essential for monitoring spread health-threatening in communities. The development multiplex assays are simultaneous such even at low concentrations. In present study, a and specific one-step droplet digital PCR (RT-ddPCR) assay developed absolute quantification influenza A (IAV), B (IBV), syncytial virus (RSV), beta-2-microglobulin transcript as an endogenous internal control (IC B2M). Results first evaluated analytical sensitivity specificity, linearity, reproducibility, recovery rates with excellent performance characteristics then applied to 37 wastewater samples previously commercially available in-house quantitative real-time reverse transcription (RT-qPCR) assays. IAV detected 16/37 (43%), IBV 19/37 (51%), RSV 10/37 (27%) samples. Direct comparison RT-qPCR showed statistically significant high agreement (kappa Cohen’s correlation coefficient: 0.834, p = 0.001) (kappa: 0.773, between two assays, while results 0.355, 0.27) good without statistical significance. Conclusions Overall, ddPCR is cost-effective, specific, can simultaneously detect three common complex matrix Due its resistance inhibitors, could be further used early warning system monitoring.

Language: Английский

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Evaluation of CLINITEST® Rapid Covid-19 + Influenza antigen test in a cohort of symptomatic patients in an emergency department

European Journal of Clinical Microbiology & Infectious Diseases, Journal Year: 2024, Volume and Issue: 43(5), P. 853 - 861

Published: Feb. 29, 2024

Language: Английский

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Clinical Evaluation of the Accuracy of the Panbio™ COVID-19/Flu A&B Rapid Panel: A Combination Antigen Rapid Diagnostic Test for the Omicron Variant and Influenza A Virus

Viral Immunology, Journal Year: 2024, Volume and Issue: 37(6), P. 317 - 321

Published: July 13, 2024

It is difficult to differentiate between coronavirus disease 2019 (COVID-19) and influenza based on the symptoms. In present study, a newly developed antigen rapid diagnostic test (Ag-RDT) called Panbio™ COVID-19/Flu A&B that can simultaneously detect severe acute respiratory syndrome 2 (SARS-CoV-2) A/B virus was evaluated. Its accuracy evaluated using 235 pairs of nasopharyngeal samples collected from patients with symptoms fever (>37.5°C). Reverse transcription polymerase chain reaction used as reference method evaluate SARS-CoV-2 detection. We confirmed Ag-RDT against Omicron variant where sensitivity specificity were 94.8% 100%, respectively. addition, identify A virus, noninferiority conducted commercial Ag-RDT, which has in comparison viral culture 98.4%, The positive negative predictive values for 98.5% 98.1%, respectively, Panbio test. evaluation this clinical suggests it high efficacy settings.

Language: Английский

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