Expression of Tissue Factor and Platelet/Leukocyte Markers on Extracellular Vesicles Reflect Platelet-Leukocyte Interaction in Severe COVID-19 DOI Open Access
Tanja Eichhorn, René Weiss, Silke Huber

et al.

Published: Oct. 16, 2023

Severe COVID-19 is frequently associated with thromboembolic complications. Increased platelet activation and platelet-leukocyte aggregate formation can amplify thrombotic responses by inducing tissue factor (TF) expression on leukocytes. Here, we characterized TF-positive extracellular vesicles (EVs) their cellular origin in 12 patients suffering from severe (time course, 134 samples overall) 25 healthy controls. EVs exposing phosphatidylserine (PS) were flow cytometry. Their was determined staining anti-CD41, anti-CD45, anti-CD235a, anti-CD105 as platelet, leukocyte, red blood cell, endothelial markers. We further investigated the association of TF, 4 (PF4), C-reactive protein (CRP) high mobility group box-1 (HMGB-1). showed higher levels PS-exposing compared to The majority these originated platelets. A amount patient CRP, HMGB-1, PF4, donors. In samples, 16.5% all CD41+ displayed leukocyte marker CD45, 55.5% EV aggregates (CD41+CD45+) co-expressed which reflects interaction platelets leukocytes an level.

Language: Английский

Circulating Nucleosomes as a Novel Biomarker for Sepsis: A Scoping Review DOI Creative Commons
Fuhong Su, Anthony Moreau, Marzia Savi

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1385 - 1385

Published: June 21, 2024

Circulating nucleosome levels are commonly elevated in physiological and pathological conditions. Their potential as biomarkers for diagnosing prognosticating sepsis remains uncertain due, part, to technical limitations existing detection methods. This scoping review explores the possible role of concentrations diagnosis, prognosis, therapeutic management sepsis. A comprehensive literature search Cochrane Medline libraries from 1996 1 February 2024 identified 110 potentially eligible studies, which 19 met inclusion criteria, encompassing a total 39 SIRS patients, 893 280 septic shock 117 other ICU control 345 healthy volunteers. The enzyme-linked immunosorbent assay [ELISA] was primary method measurement. Studies consistently reported significant correlations between NET biomarkers. Nucleosome were higher patients with than volunteers associated disease severity, indicated by SOFA APACHE II scores. Non-survivors had survivors. levels, therefore, show promise early markers NETosis sepsis, moderate diagnostic accuracy strong severity prognosis. However, available evidence is drawn mainly single-center, observational studies small sample sizes varied methods, warranting further investigation.

Language: Английский

Citations

6
Evaluation of bovine coronavirus in Korean native calves challenged through different inoculation routes DOI Creative Commons
Hyung-Chul Cho, Youngjun Kim, Yong-Il Cho

et al.

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: June 11, 2024

Abstract Bovine coronavirus (BCoV) is a pneumoenteric virus that can infect the digestive and respiratory tracts of cattle, resulting in economic losses. Despite its significance, information regarding BCoV pathogenesis limited. Hence, we investigated clinical signs, patterns viral shedding, changes antibody abundance, cytokine/chemokine production calves inoculated with via intranasal oral. Six clinically healthy Korean native (< 30 days old), initially negative for BCoV, were divided into oral groups monitored 15 post-infection (dpi). BCoV-infected exhibited signs such as nasal discharge diarrhea, starting at 3 dpi recovering by 12 dpi, being most common symptoms. Viral RNA was detected fecal samples from all infected calves. Nasal shedding occurred before regardless inoculation route; however, persisted longer. Although number partitions very few, identified blood two group 7 9 using digital RT-PCR analysis. The effectiveness maternal antibodies preventing replication appeared Our results showed interleukin (IL)-8 highly induced chemokine. During infection, levels IL-8, monocyte chemoattractant protein-1, macrophage inflammatory protein-1β significantly affected, suggesting these emerge potential reliable biomarkers predicting infection. This study underscores importance major pathogen causing diarrhea disease.

Language: Английский

Citations

5
Peripheral blood cytokine expression levels and their clinical significance in children with influenza DOI Open Access

Yanxia Zou,

Min Ding,

Yidan Zheng

et al.

Translational Pediatrics, Journal Year: 2025, Volume and Issue: 14(2), P. 286 - 297

Published: Feb. 1, 2025

Cytokine levels in the peripheral blood of children infected with influenza A virus are significantly associated disease diagnosis and severity. This study aimed to investigate correlation between expression six cytokines pediatric patients severity their condition. The were measured using a flow cytometric microsphere array. Statistical analysis was performed IBM SPSS Statistics 26.0 compare differences groups, P value less than 0.05 considered statistically significant. Univariate showed that interferon alpha-2 (IFN-α2), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-18 (IL-18) elevated group (P≤0.001). IL-18 higher H3N2 H1N1 (P=0.03), IFN-α2 Mycoplasma coinfection compared influenza-only (P=0.04). Notably, significant MCP-1 observed mild-moderate severe subgroups (P=0.008, P=0.01, respectively). Negative correlations found (r=-0.357, P=0.006 r=-0.329, Multifactorial logistic regression receiver operating characteristic (ROC) curve identified as independent risk factors for A. Inflammatory cytokine critical modulating immune inflammatory responses body. could serve early indicators group, this finding may aid differentiating subtypes, providing insights tailored interventions.

Language: Английский

Citations

0
The association between mortality due to COVID-19 and coagulative parameters: a systematic review and meta-analysis study DOI Creative Commons
Alireza Abdollahi,

Saeed Nateghi,

Zahra Panahi

et al.

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 2, 2024

This systematic review and meta-analysis study evaluated the association between mortality due to COVID-19 coagulative factors. A search was conducted on electronic databases including PubMed, Scopus, Web of Science from beginning pandemic until October 2024 identify relevant studies patients their laboratory findings related coagulation markers outcome. Eligibility criteria were defined based PICO framework, data extraction performed by two authors independently using a standardized sheet. Statistical analysis accomplished random effects model, heterogeneity among assessed I2 test. R RStudio used for statistical visualization. Our literature yielded 6969 studies, with 48 meeting inclusion our meta-analysis. The mean platelet count significantly lower in deceased compared survivors (20.58), while activated partial thromboplastin time (aPTT) fibrinogen levels did not show significant differences. pooled difference D-Dimer, International Normalized Ratio (INR), prothrombin (PT) survived (-2.45, -0.10, -0.84, respectively). These suggest that count, INR, PT may serve as potential indicators patients. results revealed reduction individuals when survivors. However, no distinctions observed aPTT survivor groups. On other hand, there noticeable variations estimated PT, D-Dimer levels, higher values group those who survived.

Language: Английский

Citations

2
Expression of Tissue Factor and Platelet/Leukocyte Markers on Extracellular Vesicles Reflect Platelet–Leukocyte Interaction in Severe COVID-19 DOI Open Access
Tanja Eichhorn, René Weiss, Silke Huber

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16886 - 16886

Published: Nov. 28, 2023

Severe COVID-19 is frequently associated with thromboembolic complications. Increased platelet activation and platelet-leukocyte aggregate formation can amplify thrombotic responses by inducing tissue factor (TF) expression on leukocytes. Here, we characterized TF-positive extracellular vesicles (EVs) their cellular origin in 12 patients suffering from severe (time course, 134 samples overall) 25 healthy controls. EVs exposing phosphatidylserine (PS) were flow cytometry. Their was determined staining anti-CD41, anti-CD45, anti-CD235a, anti-CD105 as platelet, leukocyte, red blood cell, endothelial markers. We further investigated the association of TF, 4 (PF4), C-reactive protein (CRP), high mobility group box-1 (HMGB-1). showed higher levels PS-exposing compared to The majority these originated platelets. A amount patient CRP, HMGB-1, PF4, TF donors. In samples, 16.5% all CD41+ displayed leukocyte marker CD45, 55.5% EV aggregates (CD41+CD45+) co-expressed which reflects interaction platelets leukocytes an level.

Language: Английский

Citations

4
Delineating sex-specific circulating host response signatures associated with COVID-19 severity and mortality. DOI Creative Commons
Nick Keur,

Antine Flikweert,

Isis Ricaño-Ponce

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(11), P. 111150 - 111150

Published: Oct. 11, 2024

Language: Английский

Citations

1
Fullerenol C60(OH)40 Nanoparticles and Ectoine Protect Human Nasal Epithelial Cells Against the Cytokine Storm After Addition of the Full-Length Spike Protein from SARS-CoV-2 DOI Creative Commons
Malwina Sosnowska, Mateusz Wierzbicki, Barbara Nasiłowska

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 12221 - 12255

Published: Nov. 1, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the nasal cavity, penetrates epithelial cells through interaction of its spike protein with host cell receptor angiotensin-converting enzyme (ACE2) and then triggers a cytokine storm. We aimed to assess biocompatibility fullerenol nanoparticles C

Language: Английский

Citations

0
Expression of Tissue Factor and Platelet/Leukocyte Markers on Extracellular Vesicles Reflect Platelet-Leukocyte Interaction in Severe COVID-19 DOI Open Access
Tanja Eichhorn, René Weiss, Silke Huber

et al.

Published: Oct. 16, 2023

Severe COVID-19 is frequently associated with thromboembolic complications. Increased platelet activation and platelet-leukocyte aggregate formation can amplify thrombotic responses by inducing tissue factor (TF) expression on leukocytes. Here, we characterized TF-positive extracellular vesicles (EVs) their cellular origin in 12 patients suffering from severe (time course, 134 samples overall) 25 healthy controls. EVs exposing phosphatidylserine (PS) were flow cytometry. Their was determined staining anti-CD41, anti-CD45, anti-CD235a, anti-CD105 as platelet, leukocyte, red blood cell, endothelial markers. We further investigated the association of TF, 4 (PF4), C-reactive protein (CRP) high mobility group box-1 (HMGB-1). showed higher levels PS-exposing compared to The majority these originated platelets. A amount patient CRP, HMGB-1, PF4, donors. In samples, 16.5% all CD41+ displayed leukocyte marker CD45, 55.5% EV aggregates (CD41+CD45+) co-expressed which reflects interaction platelets leukocytes an level.

Language: Английский

Citations

1