Whitening Efficiency of Papain and Bromelain Gels in Removing Dental Stains
Gels,
Journal Year:
2025,
Volume and Issue:
11(2), P. 100 - 100
Published: Feb. 1, 2025
This
study
aimed
to
evaluate
the
micro-nanostructure
and
color
changes
of
dental
enamel
after
treatment
with
new
gel
formulations
containing
papain
or
bromelain.
Eighty
caries-free,
extracted
human
teeth
were
randomly
divided
into
two
groups
(n
=
40)
stained
by
immersion
in
either
coffee
Tedi
juice
for
4
h
daily
over
five
consecutive
days.
After
staining,
samples
washed
stored
artificial
saliva
at
37
°C.
The
then
treated
four
different
whitening
gels
(GC,
G1,
G2,
Opalescence
15%)
daily.
Following
each
treatment,
rinsed
saliva.
Color
measured
using
a
digital
spectrophotometer
assess
CIEL*a*b*
ΔE*
Whiteness
index
(WI).
was
analyzed
SEM
AFM.
Data
statistically
one-way
ANOVA
followed
Tukey’s
HSD
test.
results
showed
that
bromelain
more
effective
than
commercial
removing
stains.
AFM
analysis
indicated
particularly
stains,
while
better
stains
from
natural
juices.
Healthy
has
Ra
value
approximately
10
nm,
which
increases
about
40
nm
staining.
Papain
restored
roughness
8
it
11
delivering
optimal
results,
ensures
15
stain
removal.
CIELAB
reveals
notable
ΔE
WI
bleaching,
revealing
is
removal
In
conclusion,
should
be
assessed
dentist
determine
most
suitable
achieving
results.
Language: Английский
Finite Element Simulation of Biomechanical Effects on Periodontal Ligaments During Maxillary Arch Expansion with Thermoformed Aligners
Journal of Functional Biomaterials,
Journal Year:
2025,
Volume and Issue:
16(4), P. 143 - 143
Published: April 17, 2025
Purpose:
This
paper
investigates
the
biomechanical
effect
of
thermoformed
aligners
equipped
with
complementary
attachments
(CBAs)
on
periodontal
ligaments
(PDLs)
during
expansion
process
maxillary
arch.
The
analysis
was
conducted
using
advanced
simulations
based
finite
element
method
(FEM).
Methods:
High-resolution
3D
CAD
models
were
created
for
four
tooth
types:
canine,
first
premolar,
second
and
molar.
Additional
developed
aligners,
CBAs,
PDLs.
These
integrated
into
a
comprehensive
FEM
model
to
simulate
clinical
rehabilitation
scenarios.
Validation
achieved
through
comparative
empirical
medical
data.
Results:
revealed
following:
displacement
0.134
mm
maximum
stress
4.822
KPa
in
amelocemental
junction.
For
0.132
at
3.273
premolar
had
0.129
1.358
1
from
junction;
molar
0.124
2.440
KPa.
Conclusions:
inclusion
CBAs
significantly
reduced
tipping
arch
expansion.
Among
tested,
vestibular
CBA
demonstrated
superior
performance,
delivering
optimal
movement
when
combined
aligners.
Significance:
techniques
provide
robust
cost-effective
alternative
vivo
experimentation,
offering
precise
reliable
insights
efficacy
approach
minimizes
experimental
variability
accelerates
evaluation
innovative
orthodontic
configurations.
Language: Английский
The DIAD Approach to Correlative Synchrotron X-ray Imaging and Diffraction Analysis of Human Enamel
Chemical & Biomedical Imaging,
Journal Year:
2024,
Volume and Issue:
2(3), P. 222 - 232
Published: March 8, 2024
The
Dual
Imaging
and
Diffraction
(DIAD)
beamline
at
Diamond
Light
Source
(Didcot,
U.K.)
implements
a
correlative
approach
to
the
dynamic
study
of
materials
based
on
concurrent
analysis
identical
sample
locations
using
complementary
X-ray
modalities
reveal
structural
detail
various
length
scales.
Namely,
underlying
principle
its
practical
implementation
allow
collocation
chosen
regions
within
their
interrogation
real-space
imaging
(radiography
tomography)
reciprocal
space
scattering
(diffraction).
switching
between
two
principal
modes
is
made
smooth
rapid
by
design,
so
that
data
collected
interlaced
obtain
near-simultaneous
multimodal
characterization.
Different
specific
photon
energies
are
used
for
each
mode,
interlacing
acquisition
steps
allows
conducting
static
experiments.
Building
demonstrated
realization
this
state-of-the-art
requires
further
refining
experimental
practice,
namely,
methods
gauge
volume
under
different
beam–sample
interaction.
To
address
challenge,
experiments
were
conducted
DIAD
devoted
human
dental
enamel,
hierarchical
structure
composed
hydroxyapatite
mineral
nanocrystals,
as
previously
affected
caries
(tooth
decay)
well
conditions
simulating
process
acid
demineralization.
Collocation
correlation
achieved
WAXS
(wide-angle
scattering),
2D
(radiographic),
3D
(tomographic)
imaging.
While
in
or
reveals
details
microstructure,
provided
statistical
nanoscale
ultrastructural
polycrystal
reciprocal-space
information
such
phase
preferred
orientation
(texture).
Careful
registration
positions
recorded
during
scans
allowed
direct
covisualization
from
modalities.
volumes
identified
visualized
tomographic
sets,
revealing
local
support
interpretation
diffraction
patterns.
present
4D
microscopy
paradigm
following
progression
demineralization
with
time-dependent
pattern
evolution
an
transferable
other
material
systems.
Language: Английский
Multi-resolution Correlative Ultrastructural and Chemical Analysis of Carious Enamel by Scanning Microscopy and Tomographic Imaging
ACS Applied Materials & Interfaces,
Journal Year:
2023,
Volume and Issue:
15(31), P. 37259 - 37273
Published: July 31, 2023
Caries,
a
major
global
disease
associated
with
dental
enamel
demineralization,
remains
insufficiently
understood
to
devise
effective
prevention
or
minimally
invasive
treatment.
Understanding
the
ultrastructural
changes
in
is
hampered
by
lack
of
nanoscale
characterization
chemical
spatial
distributions
within
tissue.
This
leads
requirement
develop
techniques
based
on
various
methods.
The
purpose
present
study
demonstrate
strength
analytic
methods
using
correlative
technique
single
sample
human
as
specific
case
test
accuracy
compare
regions
enamel.
science
different
integrated
genuinely
hierarchical
structures
carious
tissue
were
mapped
combination
focused
ion
beam
scanning
electron
microscopy
synchrotron
X-ray
tomography.
studied
fluorescence
(XRF)
and
wide-angle
small-angle
scattering
size
below
80
nm
for
ångström
nanometer
length
scales.
analysis
XRF
intensity
gradients
revealed
subtle
variations
Ca
samples
comparison
those
normal
mature
In
addition,
pathways
rod
demineralization
ptychography.
results
show
structural
modification
differing
locations.
These
reinforce
need
multi-modal
approaches
complex
hierarchically
structured
materials
interpret
materials.
approach
establishes
meticulous
platform
biomineralized
tissues
at
nanoscale,
which
adds
confidence
interpretation
time-saving
imaging
techniques.
protocol
demonstrated
here
can
be
applied
other
statistical
investigation
changes.
information
gathered
from
could
not
obtained
traditional
individual
Language: Английский
Optimization of Functional Toothpaste Formulation Containing Nano-Hydroxyapatite and Birch Extract for Daily Oral Care
Materials,
Journal Year:
2023,
Volume and Issue:
16(22), P. 7143 - 7143
Published: Nov. 13, 2023
This
research
work
aims
to
develop
functional
toothpastes
with
combined
enamel
remineralization
and
antibacterial
effects
using
nano-hydroxyapatites
(nHAPs)
birch
extract.
Eleven
(notated
as
P1-P11)
were
designed
featuring
different
concentrations
of
extract
a
constant
concentration
pure
nHAPs
or
substituted
(HAP-5%Zn,
HAP-0.23%Mg-3.9%Zn-2%Si-10%Sr,
HAP-2.5%Mg-2.9%Si-1.34%Zn).
In
vitro
assessments
involved
treating
artificially
demineralized
slices
analyzing
surface
repair
Atomic
Force
Microscopy
(AFM).
The
Agar
Disk
Diffusion
method
was
used
measure
activity
against
Enterococcus
faecalis,
Escherichia
coli,
Porphyromonas
gingivalis,
Streptococcus
mutans,
Staphylococcus
aureus.
Topographic
images
structure
roughness,
well
the
ability
nHAP
nanoparticles
form
self-assembled
layers,
revealed
excellent
restorative
properties
tested
toothpastes,
nanostructure
normalization
occurring
soon
10
days
after
treatment.
outcomes
highlighted
morphology
improvements
due
toothpaste
treatment
also
having
various
efficacious
effects.
Promising
results
obtained
P5
toothpaste,
containing
HAP-5%Zn
(3.4%)
(1.3%),
indicating
notable
good
properties.
study
represents
significant
advancement
in
oral
care
by
introducing
formulations
that
simultaneously
promote
health
through
effective
bacterial
inhibition.
Language: Английский
Advanced Time-Stepping Interpretation of Fly-Scan Continuous Rotation Synchrotron Tomography of Dental Enamel Demineralization
Chemical & Biomedical Imaging,
Journal Year:
2024,
Volume and Issue:
2(3), P. 213 - 221
Published: Feb. 8, 2024
High-resolution
spatial
and
temporal
analysis
3D
visualization
of
time-dependent
processes,
such
as
human
dental
enamel
acid
demineralization,
often
present
a
challenging
task.
Overcoming
this
challenge
requires
the
development
special
methods.
Dental
caries
remains
one
most
important
oral
diseases
that
involves
demineralization
hard
tissues
consequence
production
by
bacteria.
Enamel
has
hierarchically
organized
architecture
extends
down
to
nanostructural
level
high
resolution
study
its
evolution
in
detail.
is
dynamic
process
best
investigated
with
help
situ
experiments.
In
previous
studies,
synchrotron
tomography
was
applied
structure
at
certain
time
points
(time-lapse
tomography).
Here,
another
distinct
approach
time-evolving
studies
presented,
whereby
sample
image
reconstructed
it
undergoes
continuous
rotation
over
virtually
unlimited
angular
range.
The
resulting
(single)
data
set
contains
for
multiple
(potentially
overlapping)
intermediate
tomograms
can
be
extracted
analyzed
desired
using
time-stepping
selection
subsets
from
fly-scan
recording.
One
advantages
reduces
amount
required
collect
an
equivalent
number
single
tomograms.
Another
advantage
nominal
step
between
successive
reconstructions
significantly
reduced.
We
acidic
observed
progression
steps
smaller
than
total
acquisition
tomogram,
voxel
size
0.5
μm.
It
expected
presented
paper
useful
high-resolution
other
processes
assessing
small
structural
modifications
evolving
hierarchical
materials.
Language: Английский
Teeth Microcracks Research: Towards Multi-Modal Imaging
Bioengineering,
Journal Year:
2023,
Volume and Issue:
10(12), P. 1354 - 1354
Published: Nov. 25, 2023
This
perspective
is
an
overview
of
the
recent
advances
in
teeth
microcrack
(MC)
research,
where
there
a
clear
tendency
towards
shift
from
two-dimensional
(2D)
to
three-dimensional
(3D)
examination
techniques,
enhanced
with
artificial
intelligence
models
for
data
processing
and
image
acquisition.
X-ray
micro-computed
tomography
combined
machine
learning
allows
3D
characterization
all
spatially
resolved
cracks,
despite
locations
within
tooth
which
they
begin
extend,
arrangement
MCs
their
structural
properties.
With
photoluminescence
micro-/nano-Raman
spectroscopy,
optical
properties
chemical
elemental
composition
material
can
be
evaluated,
thus
helping
assess
integrity
at
MC
site.
Approaching
samples
having
cracks
different
perspectives
using
complementary
laboratory
natural
progression
multi-modal
imaging,
volumetric
(passive:
dimensions)
information
sample
supplemented
by
dynamic
(active:
composition,
interaction)
data.
Revelation
clearly
shows
need
re-assess
role
these
effect
on
longevity
tooth.
provides
insight
into
nature
hard
materials
contributes
better
understanding
how
bio-inspired
structures
could
designed
foresee
crack
propagation
biosolids.
Language: Английский
Multi-modal imaging of tooth microcrack
Published: Jan. 26, 2024
The
study
aimed
to
combine
an
X-ray
micro-computed
tomography
(μCT)
with
photoluminescence
(PL)
and
convolutional
neural
network
(CNN)
assisted
voxel
classification
volume
segmentation
for
tooth
structural
integrity
assessment
at
the
microcrack
site
verify
this
approach
extracted
human
teeth.
samples
were
first
examined
using
μCT
segmented
CNN
identify
enamel,
dentin,
cracks.
A
new
image
model
was
trained
based
on
"Multiclass
semantic
DeepLabV3+"
example
implemented
"TensorFlow".
Secondly,
buccal
palatal
teeth
surfaces
microcracks
sound
areas
selected
obtain
fluorescence
spectra
illuminated
wavelengths
of
325
nm
(cw)
266
(0.5
ns
pulsed).
proposed
–
in
combination
PL
reveals
possibilities
crack
area
distinct
precision
versatility
can
be
applied
all
microstructure
surface
mapping
analysis.
Language: Английский
In Vitro Investigation of Novel Peptide Hydrogels for Enamel Remineralization
Gels,
Journal Year:
2024,
Volume and Issue:
11(1), P. 11 - 11
Published: Dec. 27, 2024
This
study
investigates
the
microstructure
of
dental
enamel
following
demineralization
and
re-mineralization
processes,
using
DIAGNOdent
scores
images
obtained
via
scanning
electron
microscopy
(SEM),
atomic
force
(AFM),
microhardness
(Vickers).
The
research
evaluates
effects
two
experimental
hydrogels,
Anti-Amelogenin
isoform
X
(ABT260,
S1)
Anti-Kallikrein
L1
(K3014,
S2),
applied
to
demineralized
surfaces
over
periods
14
21
days.
involved
60
extracted
teeth,
free
from
cavities
or
other
lesions,
divided
into
four
groups:
a
positive
group
(+),
negative
(-)
groups
S1
S2.
last
three
underwent
with
37%
phosphoric
acid
for
20
min.
was
without
remineralization
treatment.
indicate
that
treated
is
more
effective
in
remineralizing
surface
compared
S2
Anti-Kallikrein.
These
findings
were
corroborated
by
SEM
AFM
images,
which
revealed
elongated
hydroxyapatite
(HAP)
nanoparticles
integrated
structures.
Demineralization
reduced
about
1/3
healthy
one.
Both
tested
hydrogels
restored
hardness,
being
than
peptides
facilitated
interaction
between
newly
added
minerals
residual
protein
binders
on
surface,
thereby
contributing
restoration.
Language: Английский