A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging

Plant Science, Journal Year: 2020, Volume and Issue: 303, P. 110765 - 110765

Published: Nov. 20, 2020

Language: Английский

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PoWRKY69‐PoVQ11 module positively regulates drought tolerance by accumulating fructose in Paeonia ostii

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(4), P. 1782 - 1799

Published: July 8, 2024

SUMMARY Drought is a detrimental environmental factor that restricts plant growth and threatens food security throughout the world. WRKY transcription factors play vital roles in abiotic stress response. However, of IIe subgroup members from family soluble sugar mediated drought response are largely elusive. In this study, we identified drought‐responsive factor, PoWRKY69, Paeonia ostii . PoWRKY69 functioned as positive regulator to with nucleus expression transcriptional activation activity. Silencing increased plants sensitivity stress, whereas conversely, overexpression enhanced tolerance plants. As revealed by yeast one‐hybrid, electrophoretic mobility shift assay, luciferase reporter assays, could directly bind W‐box element fructose‐1,6‐bisphosphate aldolase 5 ( PoFBA5 ) promoter, contributing cascade regulatory network activate expression. Furthermore, virus‐induced gene silencing assays demonstrated positively accumulating fructose alleviate membrane lipid peroxidation antioxidant defense system, these changes resulted reactive oxygen species scavenging. According two‐hybrid, bimolecular fluorescence complementation, firefly complementation imaging valine‐glutamine 11 (PoVQ11) physically interacted led an on promoter This study broadens our understanding WRKY69‐VQ11 module regulated accumulation provides feasible molecular measures create novel drought‐tolerant germplasm P.

Language: Английский

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Combining Physiology and Transcriptome to Reveal Mechanisms of Hosta ‘Golden Cadet’ in Response to Alkali Stress

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 593 - 593

Published: Feb. 15, 2025

As an ornamentally and medicinally worthy plant, Hosta plantaginea (Lam.) Aschers. has the adapted capacity to survive cold temperate monsoon climates in Northeastern China. However, its use is limited by soil alkalization of urban gardens. Our pre-experiment found that ‘Golden Cadet’ potential be alkali-tolerant. Hence, tissue-cultured seedlings were used as experimental material. Its related growth, physiology, transcripts examined reveal molecular mechanism response alkali stress. The results show development was affected In comparison with control, malondialdehyde (MDA) content increased 4.28-fold at 24th hour, superoxide dismutase (SOD) activity 49% 6th peroxidase (POD) soluble sugar (SS) 67% 30% 12th respectively. RNA-seq analysis revealed gene expressions 0 h, 6 12 21 h 48 differed after 200 mmol/L NaHCO3 treatment. During under stress, 2366 differentially expressed genes found. transcription factors MYB, AP2/ERF, WRKY activated genes. KEGG phytohormone signaling pathways, starch sucrose metabolism, phenylpropane production summary, can reduce membrane damage improving osmoregulation antioxidant capacity, increase regulate biosynthesis activating inducing signaling, mitigating effects toxicity. These findings guide investigation into tolerance plants, screening genes, selecting breeding novel alkali-tolerant cultivars.

Language: Английский

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Physiological and transcriptomic analyses of yellow horn (Xanthoceras sorbifolia) provide important insights into salt and saline-alkali stress tolerance

PLoS ONE, Journal Year: 2020, Volume and Issue: 15(12), P. e0244365 - e0244365

Published: Dec. 22, 2020

Yellow horn ( Xanthoceras sorbifolia ) is an oil-rich woody plant cultivated for bio-energy production in China. Soil saline-alkalization a prominent agricultural-related environmental problem limiting growth and productivity. In this study, we performed comparative physiological transcriptomic analyses to examine the mechanisms of X . seedling responding salt alkaline-salt stress. With exception chlorophyll content, experiments revealed significant increases all assessed indices response saline-alkali treatments. Notably, compared with stress, observed more pronounced changes electrolyte leakage (EL) malondialdehyde (MDA) levels which may contribute greater toxicity soils. total, 3,087 2,715 genes were differentially expressed treatments, respectively, among carbon metabolism, biosynthesis amino acids, starch sucrose reactive oxygen species signaling networks extensively enriched, transcription factor families bHLH, C2H2, bZIP, NAC, ERF transcriptionally activated. Moreover, relative stress activated upregulation related H + transport, indicating that regulation intracellular pH play important role coping These findings provide new insights investigating molecular underlying responses

Language: Английский

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Graphene Oxide as an Effective Soil Water Retention Agent Can Confer Drought Stress Tolerance to Paeonia ostii without Toxicity

Environmental Science & Technology, Journal Year: 2020, Volume and Issue: 54(13), P. 8269 - 8279

Published: June 16, 2020

Graphene oxide (GO) is considered to be an emerging environmental pollutant with its inevitable release into the environment. Thus, potential risks and biosafety are receiving increased attention. In this study, Paeonia ostii was exposed GO under drought stress. The results demonstrated that prevented soil water from evaporating due hydrophilic oxygen-containing functional groups did not change pH. Moreover, treatment resulted in lower increases reactive oxygen species, relative electrical conductivity free proline content, greater antioxidant enzyme activities of P. stress compared those control. And stress, higher photosynthesis, more intact mesophyll cells organelles open stomata were found treatment. Furthermore, induced changes expression patterns genes required for lignin biosynthesis, photosynthesis-antenna proteins, carbon fixation photosynthetic organisms, glyoxylate dicarboxylate metabolism. Additionally, accumulate environment electrostatic repulsion between roots. have toxic effects on effective retention agent; therefore, it could economically beneficial production plants

Language: Английский

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Impact of Cold Stress on Leaf Structure, Photosynthesis, and Metabolites in Camellia weiningensis and C. oleifera Seedlings

Horticulturae, Journal Year: 2022, Volume and Issue: 8(6), P. 494 - 494

Published: June 2, 2022

Camellia weiningensis Y. K. Li. sp. nov. (CW) is an endemic oil-tea species in Guizhou province, distributed the alpine karst area, which exhibits cold resistance and better economic characters than C. oleifera (CO). The mechanism of response CW seedlings has not been studied depth. Herein, we performed anatomical, physiological, metabolic analyses to assess impact stress on leaf structure, photosynthesis, metabolites CO seedlings. Anatomical analysis leaves showed had greater palisade thicknesses, tissue structure tightness, palisade-spongy ratio enhance chilling (4 °C) tolerance, but freezing (−4 caused loosening both Photosynthetic a reduction chlorophyll (Chl) fluorescence (Fv/Fm) photosynthetic parameters under Cold increased abscisic acid (ABA) contents species, exhibited highest ABA content −4 °C treatment. Additionally, indole-3-acetic (IAA) was also stress. An obviously distinct metabolite composition observed for different temperatures, significantly changed (SCMs) were enriched Prenol lipids, organooxygen compounds, fatty acyls main two top key SCMs, such as medicoside G, cynarasaponin F, yuccoside C, methionyl-proline downregulated CO. some associated with sugar metabolism, UDP-glucose, UDP-D-apiose, fructose 6-phosphate, higher CO, may contribute enhancing CW. Our findings are helpful explaining how adapt environments, will provide reference tolerance improvement application stress-resistant breeding areas.

Language: Английский

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Biochemical, Physiological, and Molecular Aspects of Ornamental Plants Adaptation to Deficit Irrigation

Horticulturae, Journal Year: 2021, Volume and Issue: 7(5), P. 107 - 107

Published: May 10, 2021

There is increasing concern regarding global warming and its severe impact on the farming sector food security. Incidences of extreme weather conditions are becoming more frequent, posing plants to stressful conditions, such as flooding, drought, heat, or frost etc. Especially for arid lands, there a tug-of-war between keeping high crop yields water use efficiency limited resources. This difficult task can be achieved through selection tolerant stress species by tolerance sensitive species. In this scenario, it important understand response stress. So far, staple foods vegetable crops deficit irrigation well studied. However, lack literature responses ornamental conditions. Considering importance ever-growing agricultural sector, review aims reveal defense mechanisms involved morpho-physiological, biochemical, molecular changes in plant’s irrigation.

Language: Английский

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PoWRKY71 is involved in Paeonia ostii resistance to drought stress by directly regulating light-harvesting chlorophyll a/b-binding 151 gene

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(11)

Published: Sept. 25, 2023

Although the functions of WRKY transcription factors in drought resistance are well known, their regulatory mechanisms response to by stabilising photosynthesis remain unclear. Here, a differentially expressed PoWRKY71 gene that was highly drought-treated Paeonia ostii leaves identified through transcriptome analysis. positively responded stress with significantly enhanced expression patterns and overexpressing tobacco greatly improved plant tolerance stress, whereas silencing P. resulted drought-intolerant phenotype. Furthermore, lower chlorophyll contents, photosynthesis, inhibited photosynthesis-related light-harvesting a/b-binding 151 (CAB151) were found PoWRKY71-silenced ostii. Meanwhile, homologous system indicated treatment increased PoCAB151 promoter activity. Interactive assays revealed directly bound on W-box element activated its transcription. In addition, plants demonstrated tolerance, together higher contents these indices dramatically PoCAB151-silenced The above results PoCAB151, thus via regulating chloroplast homeostasis content under stress. This study reveals novel drought-resistance mechanism provides feasible strategy for improving photosynthesis.

Language: Английский

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Effects of fulvic acid on the photosynthetic and physiological characteristics of Paeonia ostii under drought stress

Plant Signaling & Behavior, Journal Year: 2020, Volume and Issue: 15(7), P. 1774714 - 1774714

Published: June 4, 2020

Paeonia ost ii has become an economically important oil crop in recent years, but its growth is seriously affected by drought stress dry areas. In this study, the alleviating effect of fulvic acid (FA) on potted P. ostii under natural was investigated. The adopted experiment mainly characterized low soil water content, and roots plants cannot absorb enough to compensate for consumption transpiration, which affects normal physiological activities causes damage. results showed that FA treatment significantly increased leaf content antioxidant enzyme decreased reactive oxygen species (ROS) accumulation, proline (Pro) relative electrical conductivity (REC). Moreover, improved photosynthetic parameters chlorophyll (Chl) fluorescence parameters, maintained integrity chloroplasts mesophyll cells, expression level drought-tolerant genes. These indicated could induce enzymes eliminate ROS, reduce membrane lipid peroxidation decrease damage photosynthesis stress, would provide a measure caused stress.

Language: Английский

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Transcriptomic and physiological analysis reveals interplay between salicylic acid and drought stress in citrus tree floral initiation

Planta, Journal Year: 2021, Volume and Issue: 255(1)

Published: Dec. 20, 2021

Language: Английский

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