Exploring Endophytic Fungi from Humulus lupulus L. for Biocontrol of Phytopathogenic Fungi DOI Creative Commons
Claudia Riccioni, Beatrice Belfiori,

Maurizio Cenci

et al.

Diversity, Journal Year: 2025, Volume and Issue: 17(2), P. 94 - 94

Published: Jan. 28, 2025

Humulus lupulus L. (hop) is a crucial crop within the brewing industry and rich source of bioactive compounds. Traditionally concentrated in northeast regions Europe, hop cultivation has expanded towards southern territories such as Italy over recent decades. Managing phytosanitary threats Mediterranean climates poses challenges due to limited knowledge registered agrochemicals. In pursuit eco-friendly alternatives for disease management, we isolated 262 endophytic fungal strains from wild roots, stems, leaves, flowers. Through phylogenetic analyses, identified 51 operational taxonomic units. Dominant species Ilyonectria macrodidyma, Penicillium sp., Diaporthe columnaris, Plectosphaerella cucumerina, Fusarium oxysporum were exclusive roots. contrast, Alternaria spp. Epicoccum prevalent other tissues, Botrytis cinerea was exclusively detected female We tested seven isolates—Epicoccum Aureobasidium pullulans, Stemphylium vesicarium, Periconia byssoides, Talaromyces wortmannii, Nigrospora sphaerica—against four phytopathogenic fungi oxysporum, cinerea, Sclerotinia sclerotiorum. All endophytes exhibited antagonistic effects against at least one pathogen, with cucumerina showing strongest inhibition sp. This study marks first exploration various tissues. ex situ conserved future bioactivity assessments biotechnological applications. Original data key relevance environmentally friendly management plant diseases are provided.

Language: Английский

Chemistry and microbial sources of curdlan with potential application and safety regulations as prebiotic in food and health DOI
Deepak Kumar Verma, Alaa Kareem Niamah, Ami R. Patel

et al.

Food Research International, Journal Year: 2020, Volume and Issue: 133, P. 109136 - 109136

Published: March 2, 2020

Language: Английский

Citations

102
Therapeutic and Industrial Applications of Curdlan With Overview on Its Recent Patents DOI Creative Commons
Vinay Chaudhari, Harpal S. Buttar,

Siddhi Bagwe‐Parab

et al.

Frontiers in Nutrition, Journal Year: 2021, Volume and Issue: 8

Published: June 28, 2021

Curdlan is an exopolysaccharide, which composed of glucose linked with β-(1,3)-glycosidic bond and produced by bacteria, such as Alcaligenes spp., Agrobacterium Paenibacillus Rhizobium Saccharomyces cerevisiae, Candida fungal sources like Aureobasidium pullulan, Poria cocos , etc. has been utilized in the food pharmaceutical industries for its prebiotic, viscosifying, water-holding properties decades. Recently, usefulness curdlan further explored industry potential therapeutic applications. exhibited immunoregulatory antitumor activity preclinical settings. It was observed that can prevent proliferation malarial merozoites vivo ; therefore, it may be considered a promising therapy treatment end-stage malaria. In addition, demonstrated potent antiviral effects against human immunodeficiency virus (HIV) Aedes aegypti virus. suggested virucidal curdlans should extended other deadly viruses, severe acute respiratory syndrome (SARS), Middle East (MERS), current coronavirus-2 (SARS-CoV-2/COVID-19). The prebiotic property would confer beneficial on host promoting growth healthy microbiota gut consequently help to reduce gastrointestinal disorders. Therefore, employed manufacture prebiotics management various dysbiosis problems. Studies mechanism action curdlan-induced suppression microbial tumor cells at cellular molecular levels not only enhance our understanding regarding effectiveness but also discovery new drugs dietary supplements. primary focus this review highlight interventions anticancer, anti-malaria, antiviral, antibacterial agent humans. provides latest information about chemistry biosynthesis applications making novel dairy products, functional foods, nutraceuticals details recent patents derivatives.

Language: Английский

Citations

59
Why Do We Need Alternative Methods for Fungal Disease Management in Plants? DOI Creative Commons
Michael S. McLaughlin,

Maria Roy,

Pervaiz A. Abbasi

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(22), P. 3822 - 3822

Published: Nov. 10, 2023

Fungal pathogens pose a major threat to food production worldwide. Traditionally, chemical fungicides have been the primary means of controlling these pathogens, but many recently come under increased scrutiny due their negative effects on health humans, animals, and environment. Furthermore, use can result in development resistance populations phytopathogenic fungi. Therefore, new environmentally friendly alternatives that provide adequate levels disease control are needed replace fungicides—if not completely, then at least partially. A number conventional developed, including plant defence elicitors (PDEs); biological agents (fungi, bacteria, mycoviruses), either alone or as consortia; biochemical fungicides; natural products; RNA interference (RNAi) methods; breeding. This article reviews alternative methods available manage fungal discusses strengths weaknesses, identifies potential areas for future research.

Language: Английский

Citations

37
Recent innovations (2020–2023) in the approaches for the chemical functionalization of curdlan and pullulan: A mini-review DOI
Showkat Ali Ganie, Luqman Jameel Rather, Mohammed A. Assiri

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 260, P. 129412 - 129412

Published: Jan. 22, 2024

Language: Английский

Citations

11
Bioprotection strategies in winemaking DOI
Paola Di Gianvito, Vasileios Englezos, Kalliopi Rantsiou

et al.

International Journal of Food Microbiology, Journal Year: 2022, Volume and Issue: 364, P. 109532 - 109532

Published: Jan. 10, 2022

Language: Английский

Citations

37
Biocontrol of Non-Saccharomyces Yeasts in Vineyard against the Gray Mold Disease Agent Botrytis cinerea DOI Creative Commons

Alice Agarbati,

Laura Canonico,

Tania Pecci

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(2), P. 200 - 200

Published: Jan. 18, 2022

Botrytis cinerea (B. cinerea) is responsible for grape infection and damage to the winemaking table sectors. Although anti-Botrytis chemicals are available, they considered unsustainable resistance phenomenon adverse effects on environment human health. Research focused developing alternative approaches, such as exploiting biological control agents (BCAs). In this context, 19 yeasts of genera Cryptococcus, Aureobasidium, Metschnikowia, Kluyveromyces Wickerhamomyces were tested antimicrobial against B. development.A combination in vitro tests based dual-culture methods, volatile organic compound production assay, laboratory berries (punctured sprayed with yeasts) field experiments yeast treatments grapes vineyards allowed selection two potential BCAs.M. pulcherrima DiSVA 269 A. pullulans 211 exhibited best ability contain development cinerea, showing severity, decay McKinney index lower than a commercial formulation consisting mixture different strains, which used positive controls.The results indicated that selected strains effective BCA candidates counteract field, applying them partial or total replacement conventional treatments.

Language: Английский

Citations

35
Biological Control of Aspergillus flavus by the Yeast Aureobasidium pullulans In Vitro and on Tomato Fruit DOI Creative Commons
Izabela Podgórska-Kryszczuk

Plants, Journal Year: 2023, Volume and Issue: 12(2), P. 236 - 236

Published: Jan. 4, 2023

Aspergillus flavus is an important pathogenic fungus affecting many crops and one of the main sources their aflatoxin contamination. The primary method limiting this pathogen using chemical fungicides, but researchers focus on searching for other effective agents its control due to disadvantages limitations these agrochemicals. results obtained in present study indicate high potential two yeast strains, Aureobasidium pullulans PP4 A. ZD1, biological flavus. Under vitro conditions, mycelial growth was reduced by 53.61% 63.05%, spore germination inhibited 68.97% 79.66% ZD1 respectively. Both strains produced lytic enzymes chitinase β-1,3-glucanase after 5 days cultivation with cell wall preparations (CWP) medium as a carbon source. In addition, tested yeasts showed ability grow over wide range temperatures (4–30 °C), pH (4–11), salinity (0–12%) tolerance fungicides at concentrations corresponding field conditions. isolates were highly tolerant cupric oxychloride, showing biomass gains 85.84% (ZD1) 87.25% (PP4). Biomass presence azoxystrobin 78.71% 82.65% (PP4), while difenoconazole, it 70.09% 75.25% also antagonistic effects against directly tomato fruit. acted effectively reducing lesion diameter from 29.13 mm (control) 8.04 (PP4) 8.83 (ZD1).

Language: Английский

Citations

19
Aureobasidium spp.: Diversity, Versatility, and Agricultural Utility DOI Creative Commons
Alessandra Di Francesco, Janja Zajc, Johan A. Stenberg

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(1), P. 59 - 59

Published: Jan. 3, 2023

The black yeast-like fungi Aureobasidium spp. are ubiquitous microorganisms found in a wide variety of extreme and benign environments as saprophytes, endophytes, pathogens. Since this diverse genus includes species with potential uses agriculture the food industry, it is important that we explore their evolution spread context climate change. known to be capable producing plethora various metabolites, many which find applications field control plant present review aims explain how these can provide ecological safe strategies might adopted agricultural production systems processing. versatility lie perfectly within Sustainable Development Goals Agenda 2021–2030 by opening new horizons respectful environment human health.

Language: Английский

Citations

19
Combined omics expose microbial niches of fungi and bacteria correlating with wine volatile profiles in Douro wine region DOI Creative Commons
Viviana Martins, Ricardo López, António Teixeira

et al.

LWT, Journal Year: 2024, Volume and Issue: 193, P. 115769 - 115769

Published: Jan. 16, 2024

Wine microbial communities establish complex ecological ecosystems that modulate the formation of aroma compounds, but only a few studies sought for correlations between specific microorganisms and wine volatiles. The present study combined metabarcoding metabolomics identifying niches fungi bacteria correlating with volatile profiles wines 3 renowned cultivars emblematic Douro region. Three major were identified throughout spontaneous fermentation processes, Hanseniaspora-Saccharomyces succession timing was cultivar-dependent. largest niche included Hanseniaspora, Aureobasidium, Alternaria, Rhodotorula, Sporobolomyces, Massilia, Bacillus, Staphylococcus Cutibacterium, positively correlated 7 metabolites, namely, acetoin, isoamyl acetate, ethyl propanoate, c-3-hexenol, phenylethyl acetate 4-ethylphenol. fermentative yeasts S. cerevisiae, Torulaspora delbrueckii Meyerozyma caribbica strongly γ-butyrolactone, t-whiskylactone, alcohol, decanoate, isobutyrate, diethyl succinate, isovaleric acid, 4-ethylguaiacol 4-propylguaiacol. Lachancea quebecensis clustered several pathogenic (Penicillium citrinum, Erysiphe necator, Sclerotinia sclerotiorum, Aspergillus, Mycosphaerella tassiana) (Pseudomonas spp., Bacteroides acidifaciens, Pantoea, Stenotrophomonas Enhydrobacter), various monoterpenols norisoprenoids including linalool β-ionone, besides benzyl diacetyl, isobutyl ethyl-vanillate methyl vanillinate. Metabolite-microbiota denoted cultivar specificities likely underlying regional aromatic signatures.

Language: Английский

Citations

7
Fungal Endophytes as Biocontrol Agents against the Main Soil-Borne Diseases of Melon and Watermelon in Spain DOI Creative Commons
Vicente González García,

Eugenia Armijos,

Ana Garcés Claver

et al.

Agronomy, Journal Year: 2020, Volume and Issue: 10(6), P. 820 - 820

Published: June 9, 2020

Watermelon and melon crops are affected by some important soil-borne fungal diseases like carbonaceous rot (Macrophomina phaseolina), collapse (Monosporascus cannonballus), or the most pathology at an economic level, Fusarium wilt (Fusarium oxysporum f. sp. niveum, F. melonis, solani cucurbitae, Neocosmospora falciformis, N. keratoplastica). The methods commonly used for their control often ineffective, thus new approaches, as use of biological agents, constantly being sought. This work aimed to isolate, identify, test endophytic fungi antagonistic properties against three mentioned diseases. For this, about 350 strains were isolated from asymptomatic watermelon plants. Among these, 7 species selected evaluate potential 14 pathogens. Dual culture assays allowed select two Trichoderma according high inhibition rates observed (up 93%), that further employed in plants, showing pathogens controlled terms disease incidence, exhibiting a decrease up 67% T. lentiforme. In addition, concentrations Epicoccum purpurascens extract was germicide effect, obtaining significant differences growth depending on fermentation parameters.

Language: Английский

Citations

45