Microbial Biofungicides as a Substitute for Chemical Fungicides in the Control of Phytopathogens: Current Perspectives and Research Directions

Scientifica, Journal Year: 2024, Volume and Issue: 2024, P. 1 - 12

Published: Feb. 28, 2024

These days, two important issues are causing concern in the global community: alarmingly growing trend of human population and issue food security. To this end, people around world have been searching for solutions that could feed needy a sustainable way. In response to urgent call, scientists from started working on increasing crop production productivity by controlling pathogens harm crops. Synthetic fungicides use diseases several decades, but later, due evidenced side effects fungicides, there attempts shift towards less cost-effective eco-friendly method diseases, so far, many remarkable results achieved. However, effective shorter shelf life microbial biofungicides, as well accessibility these biofungicides growers world, it became difficult remove totally market. minimize problem, researchers suggested an integrated approach: combination with reduced dose synthetic fungicides. Hence, review explored status merits demerits compared

Language: Английский

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Metschnikowia pulcherrima in Cold Clarification: Biocontrol Activity and Aroma Enhancement in Verdicchio Wine

Fermentation, Journal Year: 2023, Volume and Issue: 9(3), P. 302 - 302

Published: March 20, 2023

Non-Saccharomyces wine yeasts are not only proposed to improve the sensory profile of but also for several distinctive promising features. Among them, biocontrol action at different steps production chain could be a suitable strategy reduce use sulfur dioxide. In this work, activity selected strain Metschnikowia pulcherrima was evaluated as inoculum in cold clarification with aim SO2 and aromatic wine. Fermentation processes were carried out winery level two consecutive vintages using pied de cuve starter coming from indigenous Saccharomyces cerevisiae strains. M. revealed an effective bio-protectant during pre-fermentative stage even if timely appropriate years permitted control wild fermentation trials. general, main oenological characters did show differences compared un-inoculated trial, while pucherrima determined enhancement ethyl hexanoate, isobutanol, acetaldehyde, geraniol they considered amounts each year. Indeed, analytical profiles wines influenced by vintage variation population. Nonetheless, overall results indicated that led improvement

Language: Английский

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Antifungal activity of non-conventional yeasts against Botrytis cinerea and non-Botrytis grape bunch rot fungi

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 23, 2022

Grapes harbour a plethora of non-conventional yeast species. Over the past two decades, several species have been extensively characterised and their contribution to wine quality is better understood. Beyond fermentation, some investigated for potential as alternative biological tools reduce grape spoilage. However, such studies remain limited few genera. This work aimed evaluate antagonistic activity must-derived yeasts against Botrytis cinerea non-Botrytis bunch-rotting moulds further elucidate mechanisms conferring antifungal activity. A total 31 strains representing 21 were screened on different agar media using dual culture technique liquid mixed cultures, respectively. Pichia kudriavzevii was most potent with minimum inhibitory concentration 102 cells/mL B. but it had narrow spectrum. Twelve displayed broad activity, inhibiting three (B05. 10, IWBT FF1 FF2), strain Aspergillus niger Alternaria alternata. Production chitinases glucanases in presence common feature antagonists. Volatile non-volatile compounds produced by analysed identified gas chromatography mass spectrometry, The volatile belonged mainly higher alcohols, esters, organosulfur monoterpenes while cyclic peptides diketopiperazine. To our knowledge, this first report demonstrate effect various

Language: Английский

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Biocontrol of post-harvest tomato rot caused by Alternaria arborescens using Torulaspora indica

Biocontrol Science and Technology, Journal Year: 2023, Volume and Issue: 33(2), P. 115 - 132

Published: Jan. 9, 2023

An important concern in modern agriculture is the production of healthy foods. This has resulted an increasing number studies on alternative plant protection strategies such as biological control. Yeasts have received considerable attention control agents against postharvest fungal diseases fruits and vegetables. Here, we describe potential yeast strain Torulaspora indica a agent Alternaria arborescens, causal tomato decay. In vitro vivo assays were used to evaluate interaction (CCA5S55) arborescens (ABS2). was followed by evaluation putative mechanisms controlling development. Our results suggest that volatile compounds competition for nutrients are which T. inhibits A. growth vitro. The indicate reduction arborescens-induced lesions presence indica.

Language: Английский

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Understanding bud rot development, caused by Botrytis cinerea, on cannabis (Cannabis sativa L.) plants grown under greenhouse conditions

Botany, Journal Year: 2023, Volume and Issue: 101(7), P. 200 - 231

Published: May 24, 2023

Botrytis cinerea is a widespread necrotrophic plant pathogen that causes diseases on >1000 species, including vegetables and ornamental greenhouse crops. On cannabis ( Cannabis sativ L.), the responsible for causing “bud rot”, major disease affecting inflorescences (compound flowers), as well seedling damping-off leaf blight under certain conditions. During cultivation, can destroy rapidly optimal relative humidity conditions (>70%) moderate temperatures (17–24 °C). Little currently known about host–pathogen interactions of cannabis. Information gleaned from other hosts provide valuable insights comparative purposes to understand development, epidemiology, pathogenicity This review describes pathogenesis host responses infection assesses potential mechanisms involved in resistance. The effects microclimatic environmental strategies early detection using prediction models, application biological control agents prevent development are discussed. Other management approaches reduce impact bud rot also reviewed. Numerous opportunities conducting additional research better cannabis– interaction identified.

Language: Английский

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Grape berry native yeast microbiota: advancing trends in the development of sustainable vineyard pathogen biocontrol strategies

OENO One, Journal Year: 2024, Volume and Issue: 58(1)

Published: Jan. 9, 2024

Grape berry is an ecological niche for a myriad of microbes, whose interactions with one another modulate fruit health and can play role in fermentation, imparting character distinctiveness to wines. With the growing concerns about awareness risks associated overuse chemical pesticides viticulture, microbial-based pest control increasingly encouraged as more sustainable environmentally friendly strategy. The use yeasts from grape berries promising alternative vineyard diseases their increasing acceptance rapidly changing our perception fungicides. In this paper, we provide overview latest methodologies characterising dynamics context disease management, discuss prospects effective biocontrol agents viticulture. Most research has focused on rots produced by Botrytis, Aspergillus, Colletotrichum Penicillium spp. using formulations that comprise single strains specific yeast genera, including Aureobasidium, Metschnikowia, Saccharomyces, Pichia, Candida Rhodotorula. However, challenging disparity between successful outcomes obtained vitro low applicability field major limitation large-scale implementation these strategies. Novel approaches maximising stability efficiency yeast-derived bioactives are discussed review.

Language: Английский

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On the Way to the Technological Development of Newly Selected Non-Saccharomyces Yeasts Selected as Innovative Biocontrol Agents in Table Grapes

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 340 - 340

Published: Feb. 6, 2024

Post-harvest decay of fresh table grapes causes considerable annual production losses. The main fungal agents both in pre- and post-harvest are B. cinerea, Penicillium spp., Aspergillus Alternaria Cladosporium spp. To date, the use agrochemicals SO2 methods to control grape molds postharvest, respectively. Significant improvements, however, have already been made apply innovative more environmentally sustainable strategies, such as Biological Control Agents (BCAs), which can reduce disease severity post-harvest. In this study, 31 new non-Saccharomyces yeast strains, isolated from berries native Apulian genotypes, were tested for their vivo effectiveness against grey mold grapes, resulting two St. bacillaris (‘N22_I1’ ‘S13_I3’), one S. diversa (‘N22_I3’), A. pullulans (‘OLB_9.1_VL’) H. uvarum (‘OLB_9.1_BR’) strains that marked efficient good BCAs. Their mechanisms action characterized through vitro assays, additional characteristics evaluated assess economic feasibility viability future technological employment. was by reducing working concentration, antagonistic effect on a wide range pathogens, ability survive formulations with long shelf life, safety human health.

Language: Английский

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Identification of grape berry indigenous epiphytic yeasts with <i>in vitro</i> and <i>in vivo</i> antagonistic activity towards pathogenic fungi

OENO One, Journal Year: 2023, Volume and Issue: 57(1), P. 253 - 264

Published: March 6, 2023

During their lifespan, grapes are exposed to conditions that can endanger plant health, including fluctuating climate and infection by pests insects. In particular, attacks filamentous fungi represent huge annual losses for grape farmers. Present concerns about rising antimicrobial resistance, fungicide prohibitions shifting consumer tastes driving the search less harmful ways of preventing damage grapevine pathogenic fungi. this study, antagonist activity yeasts isolated from berry surface microbiota was assessed in vitro vivo assays on an assortment fungal pathogens, Botrytis cinerea Aspergillus niger. The results show W. anomalus, A. pullulans, C. intermedia, R. glutinis, Z. meyerae H. takashimae were able inhibit 75 100 % mycelia growth sporulation vitro. Strains M. pulcherrima S. bacillaris also showed 50 efficacy reducing mould growth. strains further incised leaves 2-month-old grapevines ability reduce disease incidence pulcherrima, intermedia glutinis significantly reduced symptoms leaves, indicating potential as effective biocontrol vineyard.

Language: Английский

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Biocontrol and Probiotic Function of Non-Saccharomyces Yeasts: New Insights in Agri-Food Industry

Microorganisms, Journal Year: 2023, Volume and Issue: 11(6), P. 1450 - 1450

Published: May 30, 2023

Fermented food matrices, including beverages, can be defined as the result of activity complex microbial ecosystems where different microorganisms interact according to biotic and abiotic factors. Certainly, in industrial production, technological processes aim control fermentation place safe foods on market. Therefore, if safety is essential prerogative, consumers are increasingly oriented towards a healthy conscious diet driving production consequently applied research natural processes. In this regard, guarantee safety, quality diversity products should reached limiting or avoiding addition antimicrobials synthetic additives using biological approach. paper, recent re-evaluation non-Saccharomyces yeasts (NSYs) has been reviewed terms bio-protectant biocontrol with particular focus their antimicrobial power application modalities biopackaging, probiotic features promoting functional aspects. review, authors underline contribution NSYs chain role fermentative for practical useful use agent preparations.

Language: Английский

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Indigenous Yeasts for the Biocontrol of Botrytis cinerea on Table Grapes in Chile

Journal of Fungi, Journal Year: 2023, Volume and Issue: 9(5), P. 557 - 557

Published: May 11, 2023

One hundred twenty-five yeast strains isolated from table grapes and apples were evaluated for the control Botrytis cinerea of in vitro vivo. Ten selected their ability to inhibit mycelial growth B. vitro. In vivo assays, these yeasts tested at 20 °C on ‘Thompson Seedless’ berries 7 days; only three (m11, me99 ca80) because they significantly reduced incidence gray mold. These then different concentrations (1 × 107, 1 108 109 cells mL−1) grape °C. The m11, ca80 11.9, 26.1 32.1%, respectively, when submerged a suspension concentration mL−1 24 h before inoculation with cinerea. most favorable pH antifungal activity was 4.6 isolates. secreted hydrolytic enzymes chitinase β-1-glucanase, two (me99 produced siderophores. exhibited low oxidative stress tolerance strain m11 had produce biofilms. identified using 5.8S-ITS rDNA PCR-RFLP correspond Meyerozyma guilliermondii (m11) Aureobasidium pullulans species.

Language: Английский

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