Modern advances in spinal cord regeneration: hydrogel combined with neural stem cells DOI Creative Commons
Oksana Rybachuk,

Yuliia Nesterenko,

Valeriia Zhovannyk

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Severe spinal cord injuries (SCI) lead to loss of functional activity the body below injury site, affect a person’s ability self-care and have direct impact on performance. Due structural features role in body, consequences SCI cannot be completely overcome at expense endogenous regenerative potential and, developing over time, severe complications years after injury. Thus, primary task this type treatment is create artificial conditions for growth damaged nerve fibers through area SCI. Solving problem possible using tissue neuroengineering involving technology replacing natural environment with synthetic matrices (for example, hydrogels) combination stem cells, particular, neural/progenitor cells (NSPCs). This approach can provide maximum stimulation support axons neurons their myelination. In review, we consider currently available options improving condition (use NSC transplantation or/and replacement matrix, specifically hydrogel). We emphasise expediency effectiveness hydrogel matrix + NSCs complex system used reconstruction Since such (a engineering cell therapy), our opinion, allows not only creation supporting regeneration or mechanical cord, but also strengthen regeneration, prevent spread inflammatory process, promote restoration lost reflex, motor sensory functions injured cord.

Language: Английский

Whole-Heart Tissue Engineering and Cardiac Patches: Challenges and Promises DOI Creative Commons

Aram Akbarzadeh,

Soheila Sobhani,

Alireza Soltani Khaboushan

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(1), P. 106 - 106

Published: Jan. 12, 2023

Despite all the advances in preventing, diagnosing, and treating cardiovascular disorders, they still account for a significant part of mortality morbidity worldwide. The advent tissue engineering regenerative medicine has provided novel therapeutic approaches treatment various diseases. Tissue relies on three pillars: scaffolds, stem cells, growth factors. Gene cell therapy methods have been introduced as primary to cardiac engineering. Although application gene resulted improved regeneration damaged tissue, further studies are needed resolve their limitations, enhance effectiveness, translate them into clinical setting. Scaffolds from synthetic, natural, or decellularized sources desirable characteristics repair tissue. Decellularized scaffolds widely studied heart regeneration, either cell-free constructs cell-seeded platforms. human- animal-derived patches promoted through vivo vitro studies. Due complexity engineering, there is long way go before whole-heart can be routinely used practice. This paper aims review utilized Moreover, decellularization related these will discussed.

Language: Английский

Citations

16
Single-Cell Microgel Encapsulation Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in Treating Intervertebral Disc Degeneration via Inhibiting Pyroptosis DOI Creative Commons
Guanrui Huang, Haotian Shen,

Kaiwang Xu

et al.

Research, Journal Year: 2024, Volume and Issue: 7

Published: Jan. 1, 2024

While mesenchymal stem cell (MSC) shows great potentials in treating intervertebral disc degeneration, most MSC die soon after intradiscal transplantation, resulting inferior therapeutic efficacy. Currently, bulk hydrogels are the common solution to improve survival tissues, although hydrogel encapsulation impairs migration and disrupts extracellular microenvironment. Cell has been proposed overcome limitation of traditional hydrogels, yet this technique not used degeneration. Using a layer-by-layer self-assembly technique, we fabricated alginate gelatin microgel encapsulate individual for The small size allowed injection coated MSC. We demonstrated that pyroptosis was involved death under oxidative stress stimulation, coating suppressed activation by maintaining mitochondria homeostasis. Microgel protected harsh microenvironment, while retaining vital cellular functions such as migration, proliferation, differentiation. In rat model exhibits prolonged retention better efficacy attenuating compared with bare treatment alone. Further, microgel-coated exhibited improved effects degeneration via suppressing disc. For first time, microgel-encapsulated treat obtain encouraging outcomes. developed biocompatible single-cell is an effective strategy protect maintain may be efficacious approach improving therapy objective study using further understand related cytoprotective mechanisms.

Language: Английский

Citations

6
Macroporous Granular Hydrogels Functionalized with Aligned Architecture and Small Extracellular Vesicles Stimulate Osteoporotic Tendon‐To‐Bone Healing DOI Creative Commons
Wei Song, Zhijie Ma, Xin Wang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(34)

Published: Oct. 22, 2023

Osteoporotic tendon-to-bone healing (TBH) after rotator cuff repair (RCR) is a significant orthopedic challenge. Considering the aligned architecture of tendon, inflammatory microenvironment at injury site, and need for endogenous cell/tissue infiltration, there an imminent ideal scaffold to promote TBH that has architecture, ability modulate inflammation, macroporous structure. Herein, novel hydrogel comprising sodium alginate/hyaluronic acid/small extracellular vesicles from adipose-derived stem cells (sEVs) (MHA-sEVs) with immunomodulatory fabricated. When implanted subcutaneously, MHA-sEVs significantly improve cell infiltration tissue integration through its applied osteoporotic RCR model, by improving tendon while enhancing bone regeneration modulating inflammation. Notably, biomechanical strength approximately two times higher than control group, indicating great potential in reducing postoperative retear rates. Further cell-hydrogel interaction studies reveal alignment microfiber gels induces tenogenic differentiation tendon-derived cells, sEVs mitochondrial dysfunction M1 macrophages (Mφ) inhibit Mφ polarization toward via nuclear factor-kappaB (NF-κb) signaling pathway. Taken together, provide promising strategy future clinical application promoting TBH.

Language: Английский

Citations

15
Limbal stem cells carried by a four-dimensional -printed chitosan-based scaffold for corneal epithelium injury in diabetic rabbits DOI Creative Commons
Mengyuan Wang, Kaibin Liu, Xiaomin Wang

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: June 3, 2024

Methods: Herein, we obtained and characterized deltaN p63- adenosine triphosphate-binding cassette subfamily G member 2-expressing limbal stem cells (LSCs). Chitosan carboxymethyl chitosan (CTH) were cross-linked to be an in situ thermosensitive hydrogel (ACH), which was printed through four-dimensional (4D) printing obtain a porous carrier with uniform pore diameter (4D-CTH). Rabbits injected alloxan induce diabetes mellitus (DM). Following this, the LSC-carrying spread on surface of cornea diabetic rabbits cure corneal epithelium injury. Results: Compared control group (LSCs only), rapid wound healing observed treated 4D-CTH. Furthermore, test also showed better nerve repair ability. The results indicated potential 4D-CTH curing Conclusion: holds as useful tool for studying regenerative processes occurring during treatment various pathologies use cell-based technologies.

Language: Английский

Citations

4
Synergistic potential of gellan gum methacrylate and keratin hydrogel for visceral hemostasis and skin tissue regeneration DOI Creative Commons
Che-Wei Lin,

Tai-Hung Liu,

Chiun-Fan Chen

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101146 - 101146

Published: July 4, 2024

In recent years, the development of biodegradable hydrogels as an alternative over traditional wound dressing has become increasingly significant. These specific are able to offer suitable microenvironments further aid process tissue or organ regeneration. However, application in clinical medicine remains uncommon due most struggle with achieving satisfactory adhesiveness property, high mechanical support and cell compatibility simultaneously. order overcome these constraints enhance applicability hydrogels, methods have been employed this study. By reacting gellan gum methacrylic anhydride incorporating a protein, keratin, we endowed pliability via photo-polymerization chain extension, thereby obtaining hydrogel exceptional properties. Through series

Language: Английский

Citations

4
Hydrogels in cell and tissue engineering DOI
Sarah Rajabi, Sadaf Vahdat, Fatemeh Ganji

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 353 - 393

Published: Jan. 1, 2025

Citations

0
Multifunctional Bionanocomposite Hydrogels based on Gelatin Methacrylate and Polyphenolic 2D Nanoparticles Decorated with Antimicrobial Bis(imidazolium)-based Ionic Liquids DOI

Sevda Zaki-Germi,

Davood Afshar, Ali Akbari

et al.

Composites Science and Technology, Journal Year: 2025, Volume and Issue: 262, P. 111059 - 111059

Published: Jan. 20, 2025

Language: Английский

Citations

0
Porous Hydrogels from Nanoemulsion Templates With Different Structures DOI Creative Commons
Zahra Abbasian Chaleshtari, Babak Valipour Goodarzi, Reza Foudazi

et al.

Macromolecular Materials and Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract Porous hydrogels, including physical pores and spaces within the polymer network, exhibit rapid water uptake rates. Synthesizing porous hydrogels through nanoemulsion templating represents a promising avenue for development of advanced materials with tailored properties. Certain attributes, but not limited to ability tune droplet size, volume fraction, interdroplet interactions, provide tools precisely manipulate structure nanoemulsions. The main research question is how colloidal template changes characteristics network. Therefore, this study investigates preparation using concentrated nanoemulsions as templates where modulating interactions results in different pore structures properties Nanoemulsions are prepared by dispersing oil droplets continuous phase containing poly (ethylene glycol)‐diacrylate (PEGDA) sodium dodecyl sulfate (SDS). Subsequent photo‐polymerization PEGDA removal leaves behind structure. Then, Hydrogels analyzed relationship between netwrok capacities. discussion focuses on arrangement under influence altered concentrations SDS, provides confinement changing crosslink density

Language: Английский

Citations

0
Microfluidic 3D Bioprinting of Foamed Fibers with Controlled Micromorphology DOI Creative Commons
Federico Serpe, Francesco Nalin, Maria Celeste Tirelli

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: 17(9), P. 13632 - 13645

Published: Feb. 18, 2025

The synergistic integration of microfluidic technologies with additive manufacturing systems is advancing the development innovative platforms to 3D bioprint scaffolds for tissue engineering unparalleled biological relevance. Significant interest growing in realizing porous functionally graded materials (pFGMs) that can resemble hierarchical organization porosity found bone tissue. This study introduces a method fabricating based on real-time generation liquid foam, which gelled, forming fibers are organized into structured matrixes using bioprinting system. primary advantage this approach possibility adjust bubble size during printing dynamically, modifying characteristics deposited foamed filaments online and one step. As result, locally-defined tailor-made pores be distributed structures high spatial accuracy. Besides mechanical morphological characterization diverse microarchitectures, we also explored biocompatibility proposed by directly embedding osteosarcoma cells within biomaterial. Results demonstrated methodology revealed influence interior microporosity cell proliferation, highlighting potential creating tailored microenvironments. findings underscore versatility presented system its biomimetic gradients, representing substantial advancement pFGM synthesis, direct implications regenerative medicine engineering.

Language: Английский

Citations

0
3D bioprinted piezoelectric hydrogel synergized with LIPUS to promote bone regeneration DOI Creative Commons
Meng Li,

Xiantong Hu,

Xiaofei Liu

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101604 - 101604

Published: Feb. 22, 2025

Language: Английский

Citations

0