From Plants to Wound Dressing and Transdermal Delivery of Bioactive Compounds

Plants, Journal Year: 2023, Volume and Issue: 12(14), P. 2661 - 2661

Published: July 16, 2023

Transdermal delivery devices and wound dressing materials are constantly improved upgraded with the aim of enhancing their beneficial effects, biocompatibility, biodegradability, cost effectiveness. Therefore, researchers in field have shown an increasing interest using natural compounds as constituents for such systems. Plants, important source so-called “natural products” enormous variety structural diversity that still exceeds capacity present-day sciences to define or even discover them, been part medicine since ancient times. However, benefits just at beginning being fully exploited modern dermal transdermal Thus, plant-based primary compounds, without biological activity, contained gums mucilages, traditionally used gelling texturing agents food industry, now explored valuable cost-effective components biomedical field. Their non-toxicity compensate local availability compositional variations. Also, secondary metabolites, classified based on chemical structure, intensively investigated wide pharmacological toxicological effects. impact is highlighted detail through most recent reported studies. Innovative isolation purification techniques, new drug systems, advanced evaluation procedures presented.

Language: Английский

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In Situ Gelling Behavior and Biopharmaceutical Characterization of Nano-Silver-Loaded Poloxamer Matrices Designed for Nasal Drug Delivery

Gels, Journal Year: 2024, Volume and Issue: 10(6), P. 385 - 385

Published: June 5, 2024

A combination of Poloxamer 407 (P407) and hydroxypropyl methylcellulose (HPMC) hydrosols is proposed as an in situ thermo-gelling vehicle for the nasal drug delivery chlorhexidine–silver nanoparticles conjugates (SN-CX). Optimization formulation was carried out by applying varying ratios P407 HPMC presence absence SN-CX so that gelation would occur temperature range cavity (30–34 °C). Mechanisms observed phenomena were suggested based on viscosimetry, texture analysis, dynamic light scattering. Tests sprayability, washout time, vitro release, ex vivo permeation, antimicrobial activity. When applied separately, found to lower (Tg), whereas increased it. However, HPMC, interfered with micellar organization a principally contrasting way while leading even further decrease Tg. SN-CX-loaded formulations composed 16% 0.1% demonstrated desired at 31.9 °C, good sprayability (52.95% coverage anterior cavity), mucoadhesion 70 min under simulated clearance, expedient release preserved anti-infective activity against seasonal Influenza virus beta-coronavirus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus other pathogens. Our findings suggest current development could be considered potential protective spray respiratory infections.

Language: Английский

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Antimicrobial and Antiherpetic Properties of Nanoencapsulated Hypericum perforatum Extract

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 366 - 366

Published: March 4, 2025

Background/Objectives: This study aims to gain insights into the antimicrobial and antiherpetic activity of hyperforin-rich Hypericum perforatum L. (HP) extract using nanostructured lipid carriers (NLCs) as delivery platforms. Methods: Two established NLC specimens, comprising glyceryl behenate almond oil or borage oil, their extract-loaded counterparts (HP-NLCs) were utilized. Their minimal bactericidal/fungicidal concentrations (MBC; MFC) investigated against Escherichia coli ATCC 25922, Staphylococcus aureus 25923, Pseudomonas aeruginosa 10145, Klebsiella pneumoniae 10031, Candida albicans 10231. The anti-herpesvirus (HSV-1) potential was evaluated concerning antiviral virucidal impact on viral adsorption. Results: oil-based nanodispersion (HP-NLC2) exhibited pronounced microbicidal S. (MBC 6.3 mg/mL), K. 97.7 µg/mL), C. (MFC < 48.8 unlike oil-containing sample (HP-NLC1), which showed only weak inhibition fungal growth. HP-NLC2 found be less cytotoxic suppress HSV-1 replication slightly more than HP-NLC1, but generally, effects weak. Neither empty nanoparticles nor HP expressed E. coli, P. aeruginosa, extracellular virions, adhesion. Conclusions: It could concluded that both HP-NLC samples revealed minor extract, demonstrated significant antibacterial antimycotic activity. Therefore, latter featured a convenient HP-carrier system for nano-designed dermal pharmaceutical formulations. Such thorough investigation hyperforin-determined anti-HSV-1 properties, being first its kind, contributes fundamental knowledge reveals new perspectives utilization, limitations, therapeutic designation non-polar components.

Language: Английский

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Bigels: a comprehensive review of properties, preparation techniques, applications in pharmaceuticals, food, cosmetic products, and emerging patents

Journal of Dispersion Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 31

Published: March 26, 2025

In recent years, bigels (BGs) have garnered increasing attention as an innovative class of semisolid formulations, distinguished by their unique ability to combine two immiscible gels through high shear mixing, resulting in a uniform dispersion. This distinctive composition imparts BGs with wide range advantageous properties. They not only facilitate the delivery both hydrophilic and lipophilic drugs but also demonstrate rapid spreadability easy washability, which are crucial for patient comfort compliance. Furthermore, noted capacity enhance skin hydration, particularly within stratum corneum, thereby potentially improving therapeutic outcomes. review aims comprehensively explore classification, advantages, limitations, various preparation methods, characterization techniques employed BGs. Key analytical approaches discussed include microscopy detailed structural analysis, droplet size distribution assessments, Fourier transform infrared spectroscopy examine chemical properties, mechanical tests such viscosity, spreadability, texture evaluations. irritation determination zone inhibition assess antimicrobial activity ensure safety efficacy formulations. Studies on vitro drug release, ex vivo permeation experiments, stability assessments understanding performance pharmaceutical cosmetic applications. release studies help predicting kinetics from BGs, while experiments provide insights into deliver skin. Stability including physical, chemical, microbiological stability, essential long-term effectiveness bigel Despite yet being commercially available, research has shown be effective carriers controlled topical, transdermal, buccal, vaginal systems. Their incorporate makes them versatile promising candidates variety Moving forward, hold substantial promise platforms experience diverse properties multifunctional capabilities could revolutionize field delivery, paving way more patient-friendly treatments.

Language: Английский

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Characterization, Biocompatibility and Antioxidant Activity of Hydrogels Containing Propolis Extract as an Alternative Treatment in Wound Healing

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(5), P. 575 - 575

Published: April 30, 2024

Hydrogels consist of a network highly porous polymeric chains with the potential for use as wound dressing. Propolis is natural product several biological properties including anti-inflammatory, antibacterial and antioxidant activities. This study was aimed at synthesizing characterizing polyacrylamide/methylcellulose hydrogel containing propolis an active ingredient, to serve dressing alternative, treatment skin lesions. The hydrogels were prepared using free radical polymerization, characterized scanning electron microscopy, infrared spectroscopy, thermogravimetry, differential calorimetry, swelling capacity, mechanical rheological properties, UV-Vis activity by DPPH, ABTS FRAP assays biocompatibility determined in Vero cells J774 macrophages MTT assay. showed foliaceous structure well-defined network, good ability absorb water aqueous solutions simulating body fluids well desirable pseudoplastic behavior. In 1.0 2.5% propolis, contents total polyphenols 24.74 ± 1.71 mg GAE/g 32.10 1.01 those flavonoids 8.01 0.99 QE/g 13.81 0.71 QE/g, respectively, addition all three methods used. Therefore, extract, may promising alternative lesions, due their anti-oxidant low cost availability.

Language: Английский

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Tissue Regeneration and Remodeling in Rat Models after Application of Hypericum perforatum L. Extract-Loaded Bigels

Gels, Journal Year: 2024, Volume and Issue: 10(5), P. 341 - 341

Published: May 17, 2024

The wound-healing effect of St. John’s Wort (SJW) is mainly attributed to hyperforin (HP), but its low stability restricts topical administration. This study investigates how “free” HP-rich SJW extract (incorporated into a bigel; B/SJW) and “protected” by nanostructured lipid carriers (also included in biphasic semisolid; B/NLC-SJW) affect tissue regeneration rat skin excision wound model. Wound diameter, histological changes, gene expression levels fibronectin (Fn), matrix metalloproteinase 8 (MMP8), tumor necrosis factor-alpha (TNF-α) were employed quantify the healing progress. A significant size reduction was achieved after applying both extract-containing semisolids, 21-day application period, smallest observed B/NLC-SJW-treated animals. However, inflammatory response affected more favorably bigel containing extract, as evidenced studies. Moreover, B/SJW, Fn, MMP8, TNF-α significantly higher than positive control. In conclusion, formulations exhibited beneficial effects on skin, B/SJW restoration processes comprehensive efficient way.

Language: Английский

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3D Printed Bigel: A Novel Delivery System for Cannabidiol-Rich Hemp Extract

Gels, Journal Year: 2024, Volume and Issue: 10(12), P. 770 - 770

Published: Nov. 26, 2024

The therapeutic potential of Cannabis sativa L. extract has gained significant attention due to its diverse medical applications. Sublingual administration remains a common delivery method cannabinoids; however, challenges often arise the inconvenient form and taste. To address these issues, novel bigel formulation was developed, combining water oil phases enhance stability bioavailability. This incorporates cannabidiol-rich hemp extract, hyaluronic acid for moisturizing properties, taste-masking agent improve patient compliance comfort. Using standardized rich in cannabinoids well-characterized terpene profile, printability bigels evaluated through 3D printing technology. A printout with known cannabidiol (CBD) cannabidiolic (CBDA) content 11.613 mg ± 0.192 CBD 4.732 0.280 CBDA obtained. In addition, release profile determine efficiency active ingredient—dissolved ingredient levels ranged from 74.84% 0.50 80.87% 3.20 80.84 1.33 98.31 1.70 depending on formulation. Rheological studies were conducted evaluate viscosity under varying temperature conditions, ensuring their usability. Findings suggest that this 3D-printed could significantly cannabis extracts, offering more convenient effective option patients. research underscores importance innovation cannabinoid therapies paves way further advancements personalized medicine.

Language: Английский

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In silico study of the toxicity of hyperforin and its metabolites

Pharmacia, Journal Year: 2023, Volume and Issue: 70(3), P. 435 - 447

Published: July 6, 2023

St. John’s wort is a medicinal herb well-known for its antidepressant, anti-inflammatory, antimycotic, and wound-healing properties. Hyperforin, the major phloroglucinol derivative, has been implicated as one of main contributors to these therapeutic effects. Because high reactivity, this phytochemical can cause various adverse effects, such allergic reactions, dizziness, dry mouth, fatigue. To predict critical parameters hyperforin’s possible behavior after oral administration, in silico methods were applied. The pharmacokinetic profile, bioactivity, toxicity analyzed by applying Molinspiration cheminformatics, SwissADME, PreADME/Tox, OECD QSAR Toolbox software. results showed adequate absorption, affinity plasma proteins, prolonged renal excretion acylphloroglucinol. metabolic activity, reason potential cyto- genotoxicity, predicted carcinogenicity mutagenicity hyperforin, necessitate further vitro vivo tests.

Language: Английский

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Novel approaches in food grade bigels properties and applications: A review

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137424 - 137424

Published: Nov. 12, 2024

Language: Английский

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Bigel formulations of St. John’s wort extract in wound healing: toxicological aspects

Biotechnology & Biotechnological Equipment, Journal Year: 2023, Volume and Issue: 37(1)

Published: Oct. 6, 2023

This study aimed to investigate the toxicological profile of hyperforin (HP) in silico and assess it vivo after topical application an HP-rich St. John's wort (SJW) extract. The former analysis predicted low toxicity because HP's inability bind DNA or proteins, but structural alerts for skin irritation/corrosion, carcinogenicity, mutagenicity were found. Animal studies involved treatment excision wounds Wistar rats with poloxamer 407/borage oil formulations (bigels; Bs) containing SJW extract previously developed by us. effects semisolids comprising 'free' (B/SJW) loaded nanostructured lipid carriers (B/NLC-SJW) compared positive (commercial herbal product) negative (untreated) controls 2-, 7-, 14-, 21-day applications. Malondialdehyde (MDA) ABTS assays evaluated degree oxidative stress—treatment bigels did not affect MDA favorably led increased radical-cation scavenging capacity (compared controls). Gamma-glutamyl transferase (GGT), aspartate aminotransferase (ASAT), alanine (ALAT), lactate dehydrogenase (LDH) enzyme levels measured as indicators liver/tissue damage. Treatment both B/SJW B/NLC-SJW 21 days resulted lower GGT ASAT than those controls. Two-day biphasic contributed normalized ALAT (lower controls), same trends observed LDH a 7-day treatment. promising results obtained suggest that this drug delivery system may only preserve HP effectively also 'expose' its cyto-/hepatoprotective potential.

Language: Английский

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