Bioprinting of Cells, Organoids and Organs-on-a-Chip Together with Hydrogels Improves Structural and Mechanical Cues

Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1638 - 1638

Published: Oct. 1, 2024

The 3D bioprinting technique has made enormous progress in tissue engineering, regenerative medicine and research into diseases such as cancer. Apart from individual cells, a collection of organoids, can be printed combination with various hydrogels. It hypothesized that will even become promising tool for mechanobiological analyses organoids their matrix environments highly defined precisely structured environments, which the mechanical properties cell environment individually adjusted. Mechanical obstacles or bead markers integrated bioprinted samples to analyze deformations forces within these constructs, perform biophysical analysis complex systems, are still not standard techniques. review highlights advances 4D printing technologies integrating cues so next step detailed key future directions organoid generation development disease model regeneration drug testing perspective. Finally, hydrogels, pure natural synthetic hydrogels mixtures, organoid–cell co-cultures, organ-on-a-chip systems organoid-organ-on-a chip combinations introduces use assembloids determine mutual interactions different types cell–matrix interferences specific biological environments.

Language: Английский

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Chitosan: modification and biodegradability of by-products

Polymer Bulletin, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Language: Английский

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Rheology and printability of biopolymeric oil‐in‐water high internal phase Pickering emulsions: a review

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 3, 2025

Abstract Biopolymeric oil‐in‐water (O/W) high internal phase Pickering emulsions (HIPPEs) due to their unique rheological behaviors of HIPPEs such as shear‐thinning property, viscoelasticity, and thixotropic recovery have emerged highly promising printing inks in the 3D process. O/W biopolymer‐based are categorized complex fluids, where parameters crucial for optimizing printability. However, existing reviews not fully elucidated interrelationship between rheology printability enhancing quality performance printed parts. This review delved into influence factors continuous (e.g., biopolymer type, concentration, pH, ionic strength) oil volume fraction, encapsulated components) on rheology, adjust order prepare more eligible inks. Moreover, a spectrum rheology–printability relationships, derived from empirical trends rigorous analytical models, is examined provide generalized guidelines achieving successful HIPPEs. Furthermore, challenges future perspectives preparing suitable additive manufacturing were presented. Leveraging these insights significantly reduces reliance trial‐and‐error methods printing, thereby fostering robust development novel overall products.

Language: Английский

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Hydrogel-Based Inks for Extrusion 3D Printing: A Rheological Viewpoint

Current Opinion in Colloid & Interface Science, Journal Year: 2025, Volume and Issue: unknown, P. 101918 - 101918

Published: March 1, 2025

Language: Английский

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Extrusion-Based 3D Printing of Pharmaceuticals—Evaluating Polymer (Sodium Alginate, HPC, HPMC)-Based Ink’s Suitability by Investigating Rheology

Micromachines, Journal Year: 2025, Volume and Issue: 16(2), P. 163 - 163

Published: Jan. 30, 2025

Three-dimensional printing is promising in the pharmaceutical industry for personalized medicine, on-demand production, tailored drug loading, etc. Pressure-assisted microsyringe (PAM) popular due to its low cost, simple operation, and compatibility with heat-sensitive drugs but limited by ink formulations lacking essential characteristics, impacting their performance. This study evaluates inks based on sodium alginate (SA), hydroxypropyl cellulose (HPC H), methylcellulose (HPMC K100 K4) PAM 3D analyzing rheology. The included model Fenofibrate, functional excipients (e.g., mannitol, polyethylene glycol, etc.), water or water–ethanol mixtures. Pills thin films as an oral dosage were printed using a 410 μm nozzle, 10 mm/s speed, 50% infill density, 60 kPa pressure. Among various formulated inks, only containing 0.8% SA achieved successful prints desired shape fidelity, linked rheological properties, which assessed flow, amplitude sweep, thixotropy tests. concludes that (i) ink’s properties—viscosity, shear thinning, viscoelasticity, modulus, flow point, recovery, etc.—have be considered determine whether it will print well; (ii) printability independent of form; (iii) optimal are viscoelastic solids specific traits. research provides insights developing polymer-based effective pharmaceuticals.

Language: Английский

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Influence of size and crystallinity of nanohydroxyapatite (nHA) particles on the properties of Polylactic Acid/nHA nanocomposite scaffolds produced by 3D printing

Journal of Materials Research and Technology, Journal Year: 2024, Volume and Issue: 30, P. 3101 - 3111

Published: April 9, 2024

Polylactic acid (PLA) and hydroxyapatite (HA) composite scaffolds have been widely studied for applications in bone tissue engineering (BTE) due to their bioactive biocompatible properties. However, there is a need more knowledge about the influence of size crystallinity HA nanoparticles (nHA) on properties PLA/nHA nanocomposite produced by 3D printing. In this study, printing was used produce PLA incorporated with nHA filler different particle sizes crystallinities. Initially, nanocomposites were prepared casting scaffolds, which characterized analysis thermal, morphological, physical-chemical, mechanical, biological vitro. The results showed that particles mainly influenced scaffolds' mechanical properties, degradation rate, bioactivity. Incorporating provided gain compressive strength compared pure PLA, superior natural cancellous bone, varies between 2 12 MPa. lower crystallinity, 39.46 %, promoted higher rate bioactivity vitro its solubility simulated fluid. All cell viability above 90 %. suitable BTE applications.

Language: Английский

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Materials and Methods for All-Cellulose 3D Printing in Sustainable Additive Manufacturing

Sustainable Chemistry, Journal Year: 2024, Volume and Issue: 5(2), P. 98 - 115

Published: May 10, 2024

Additive manufacturing, commonly referred to as 3D printing, is an exciting and versatile manufacturing technology that has gained traction interest in both academic industrial settings. Polymeric materials are essential components a majority of the feedstocks used across various printing technologies. As environmental ramifications sole or primary reliance on petrochemicals resource for polymers continue manifest themselves global scale, transition more sustainable bioderived alternatives could offer solutions. In particular, cellulose promising due its abundance, biodegradability, excellent thermal mechanical properties, ability be chemically modified suit applications. Traditionally, native was incorporated additive applications only substrate, filler, reinforcement other because it does not melt easily dissolve. Now, exploration all-cellulose printed invigorated by new liquid processing strategies involving liquid-like slurries, nanocolloids, advances direct solvents highlight versatility desirable properties this abundant biorenewable photosynthetic feedstock. This review discusses progress approaches associated challenges, with purpose promoting future research development important future.

Language: Английский

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Hydrogels based on galactomannan and κ-carrageenan containing immobilized biomolecules for in vivo thermal-burn wound treatment

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 270, P. 132379 - 132379

Published: May 14, 2024

Language: Английский

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Investigating How All‐Trans Retinoic Acid Polycaprolactone (atRA‐PCL) Microparticles Alter the Material Properties of 3D Printed Fibrin Constructs

Macromolecular Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Abstract The 3D printing of human tissue constructs requires carefully designed bioinks to support the growth and function cells. Here it is shown that an additional parameter how drug‐releasing microparticles affect material properties scaffold. A microfluidic platform used create all‐trans retinoic acid (atRA) polycaprolactone (PCL) with a high encapsulation efficiency (85.9 ± 5.0%), incorporate them into fibrin investigate their effect on properties. An around 25–35% higher than current state art batch methods achieved. It also found drug loading concentration affects microparticle size, which can be controlled using platform. release atRA slower in buffer, presence modulates both degradation rheological constructs. Finally, exhibits stronger solid‐like atRA‐PCL microparticles. These findings establish basis for understanding interplay between scaffold materials, paving way achieve tailored mechanical properties, together sustained delivery engineering applications.

Language: Английский

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Enhanced Bioprinting of 3D Corneal Stroma Patches with Reliability, Assessing Product Consistency and Quality through Optimized Electron Beam Sterilization

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

This study focuses on the optimization of sterilization methods for bioprinted three-dimensional (3D) corneal stroma patches prepared using cornea-derived decellularized extracellular matrix (Co-dECM) hydrogels and human keratocytes, with aim enhancing clinical applications in tissue engineering. An essential aspect this is to refine processes, particularly focusing electron beam (EB) sterilization, maintain structural functional integrity Co-dECM while ensuring sterility. The reveals that EB outperformed traditional like ethylene oxide (EtO) gas autoclaving, which tend degrade biochemical properties hydrogels. By optimizing EB-sterilization process, mechanical characteristics needed successful 3D bioprinting are retained, reducing batch variability patches. Consistency production vital meeting regulatory standards patient safety. Moreover, investigates immunomodulatory sterilized hydrogels, emphasizing their potential minimize inflammatory responses, crucial maintaining keratocyte phenotype. These findings significantly advance biomedical engineering by providing a method preserves material integrity, minimizes immunogenicity, supports translation patches, offering promising alternative donor transplants synthetic substitutes.

Language: Английский

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