Experimental Cell Research, Journal Year: 2021, Volume and Issue: 402(1), P. 112511 - 112511
Published: Feb. 11, 2021
Language: Английский
Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)
Published: April 29, 2020
Abstract Single-cell RNA sequencing (scRNA-seq) provides details for individual cells; however, crucial spatial information is often lost. We present SpaOTsc, a method relying on structured optimal transport to recover properties of scRNA-seq data by utilizing measurements relatively small number genes. A metric cells in first established based map connecting it with the measurements. The cell–cell communications are then obtained “optimally transporting” signal senders target receivers space. Using partial decomposition, we next compute intercellular gene–gene flow estimate regulations between genes across cells. Four datasets employed cross-validation gene expression prediction and comparison known communications. SpaOTsc has broader applications, both integrating non-spatial single-cell data, directly transcriptomics reconstruct cellular dynamics tissues.
Language: Английский
Citations
296
Cell Reports, Journal Year: 2020, Volume and Issue: 32(10), P. 108121 - 108121
Published: Sept. 1, 2020
The frizzled (fz) and dishevelled (dsh) genes are highly conserved members of both the planar cell polarity (PCP) pathway Wnt signaling pathway. Given these dual functions, several studies have examined whether ligands provide a tissue-scale orientation cue for PCP establishment during development, reached differing conclusions. Here, we re-examine this issue in Drosophila melanogaster wing notum using split-Gal4 co-expression analysis, multiplex somatic CRISPR, double RNAi experiments. Pairwise loss-of-function experiments targeting wg together with other genes, via CRISPR or RNAi, do not produce defects notum. In addition, against evi (aka wntless), which is required secretion ligands, did detectable phenotypes. Altogether, our results support hypothesis that contribute to
Language: Английский
Citations
52
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9431 - 9431
Published: May 29, 2023
Disruption of endometrial regeneration, fibrosis formation, and intrauterine adhesions underlie the development "thin" endometrium and/or Asherman's syndrome (AS) are a common cause infertility high risk for adverse obstetric outcomes. The methods used (surgical adhesiolysis, anti-adhesive agents, hormonal therapy) do not allow restoration regenerative properties endometrium. experience gained today with cell therapy using multipotent mesenchymal stromal cells (MMSCs) proves their proliferative in tissue damage. Their contribution to processes is still poorly understood. One these mechanisms based on paracrine effects MMSCs associated stimulation microenvironment by secreting extracellular vesicles (EVs) into space. EVs, whose source MMSCs, able stimulate progenitor stem damaged tissues exert cytoprotective, antiapoptotic, angiogenic effects. This review described regulatory pathological conditions decrease it presented available data from studies effect EVs repair processes, involvement human reproductive at level implantation embryogenesis.
Language: Английский
Citations
18
Insect Molecular Biology, Journal Year: 2024, Volume and Issue: 33(5), P. 467 - 480
Published: Feb. 9, 2024
The function of DNA methylation in insects and the methyltransferase (Dnmt) genes that influence remains uncertain. We used RNA interference to reduce gene expression Dnmt1 within whitefly Bemisia tabaci (Hemiptera:Aleyrodidae; Gennadius), a hemipteran species relies on for proper gametogenesis. then RNA-seq test an priori hypothesis meiosis-related genetic pathways would be perturbed. generally did not find overall effect pathways. However, we found Wnt pathway, associated with entry into meiosis vertebrates, were differentially expressed. Our results are consistent knockdown influencing specific causing general transcriptional response. This is finding also seen other insect species. characterised methylome B. assessed cytosine methylation. has characteristics comparable hemipterans regarding level, enrichment bodies, bimodal distribution methylated/non-methylated genes. Very little differential was observed, difference differences expression. presents interesting new candidate pathway future studies.
Language: Английский
Citations
7
Current Opinion in Cell Biology, Journal Year: 2019, Volume and Issue: 60, P. 27 - 35
Published: April 20, 2019
Language: Английский
Citations
44
Molecular Reproduction and Development, Journal Year: 2021, Volume and Issue: 88(6), P. 379 - 394
Published: May 20, 2021
Endometrial damage is an important cause of female reproductive problems, manifested as menstrual abnormalities, infertility, recurrent pregnancy loss, and other complications. These conditions are collectively termed "Asherman syndrome" (AS) typically associated with induced terminations, repeated diagnostic curettage intrauterine infections. Cancer treatment also has unexpected detrimental side effects on endometrial function in survivors independently ovarian effects. stem cells act the regeneration endometrium repair through direct differentiation or paracrine Nonendometrial adult cells, such bone marrow-derived mesenchymal umbilical cord-derived autologous allogenic applications, can injured tissue animal models AS human studies. However, there remains a lack research damaged after reversal tumors, especially cancers. Here, we review biological mechanisms regeneration, progress challenges for cell therapy endometrium, discuss potential applications their use cancer remission, Successful application will improve parameters patients cancer. Significance: The fertile ground embryos, but to greatly impair fertility. Adult combined engineering scaffold materials not have made great repairing due benign lesions. caused by malignant tumors tumor therapies, safety effectiveness cell-based therapies need be further explored. This focuses molecular insights clinical discusses possible difficulties that overcome survivors. development cell-related new programs help safely effectively meet fertility needs
Language: Английский
Citations
40
Cells, Journal Year: 2021, Volume and Issue: 10(6), P. 1454 - 1454
Published: June 10, 2021
Throughout oogenesis, Drosophila egg chambers traverse the fine line between survival and death. After surviving ten early middle stages of drastically change their size structure to produce fully developed oocytes. The development an oocyte comes at a cost, price is lives oocyte’s 15 siblings, nurse cells. These cells do not die own accord. Their death dependent upon neighbors—the stretch follicle Stretch are nonprofessional phagocytes that spend final oogenesis surrounding subsequently forcing give up everything for sake oocyte. In this review, we provide overview cell in ovary, with focus on recent findings concerning phagocyte-dependent non-autonomous
Language: Английский
Citations
35
The Journal of Cell Biology, Journal Year: 2018, Volume and Issue: 217(11), P. 3965 - 3976
Published: Oct. 12, 2018
β-Catenin functions as an adherens junction protein for cell-cell adhesion and a signaling protein. β-catenin function is dependent on its stability, which regulated by protein-protein interactions that stabilize or target it proteasome-mediated degradation. In this study, we show stability intracellular pH (pHi) dynamics, with decreased at higher pHi in both mammalian cells Drosophila melanogaster degradation requires phosphorylation of N-terminal residues recognition the E3 ligase β-TrCP. While was independent, induced increased β-TrCP binding stability. An evolutionarily conserved histidine (found DSGIHS destruction motif) required pH-dependent to Expressing cancer-associated H36R-β-catenin mutant eye sufficient induce Wnt produced pronounced tumors not seen other oncogenic alleles. We identify dynamics previously unrecognized regulator functioning coincidence phosphorylation.
Language: Английский
Citations
46
Non-coding RNA Research, Journal Year: 2023, Volume and Issue: 8(3), P. 273 - 281
Published: March 9, 2023
Cancer stem cells (CSCs) identified in lung cancer exhibit resistance to chemotherapy, radiotherapy, and targeted therapy. Therefore, a technology for controlling CSCs is needed overcome such Various evidences about the association between epithelial-mesenchymal transition related transcriptomic alteration acquisition of CSC phenotype have been proposed recently. Down-regulated miR-26a-5p closely mesenchymal-like cell lines. These findings suggest that might be involved stemness. RNA polymerase III subunit G (POLR3G) was selected as candidate target It found directly regulates expression POLR3G.Overexpression induced marked reduction colony formation sphere formation. Co-treatment paclitaxel decreased growth, suggesting play role chemotherapy sensitizer. In genome atlas data, high low POLR3G were also higher survival rate patients with adenocarcinoma. results can suppress stemness make become sensitive chemotherapy. This finding provides novel insight into potential treatment by regulating
Language: Английский
Citations
13
Insects, Journal Year: 2024, Volume and Issue: 15(7), P. 491 - 491
Published: June 30, 2024
Drosophila is a well-established insect model system for studying various physiological phenomena and developmental processes, with focus on gene regulation. development controlled by programmed regulatory mechanisms specific to individual tissues. When key processes are shared among insects, the associated networks believed be conserved across insects. Thus, studies of regulation in have substantially contributed our understanding development. Over past two decades, microRNAs (miRNAs) revealed their crucial roles processes. This review focuses biological miRNAs tissues Additionally, as future direction, we discuss sequencing technologies that can analyze interactions between target genes, aim enhancing miRNA
Language: Английский
Citations
4