Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: March 1, 2024
Aberrant
glycosylation
patterns
of
glycoproteins
and
glycolipids
have
long
been
recognized
as
one
the
major
hallmarks
cancer
cells
that
has
led
to
numerous
glycoconjugate
vaccine
attempts.
These
abnormal
profiles
mostly
originate
from
lack
key
glycosyltransferases
activities,
mutations,
over
expressions,
or
modifications
requisite
chaperone
for
functional
folding.
Due
their
relative
structural
simplicity,
O
-linked
glycans
altered
mucin
family
particularly
attractive
in
design
tumor
associated
carbohydrate-based
vaccines.
Several
such
formulations
generated
potent
monoclonal
anti-carbohydrate
antibodies
useful
diagnostic
immunotherapies
fight
against
cancer.
Paradoxically,
related
enveloped
viruses
also
express
analogous
N
-
patterns.
However,
due
fact
are
not
equipped
with
appropriate
glycosyl
enzyme
machinery,
they
need
hijack
infected
host
cells.
Although
resulting
very
similar
those
normal
cells,
some
glycan
often
share
common
simplicity
identified
on
Consequently,
given
both
viral
glycoepitopes,
vaccines
could
be
highly
generate
immune
responses
target
conditions.
Journal of Virological Methods,
Journal Year:
2020,
Volume and Issue:
287, P. 114008 - 114008
Published: Nov. 5, 2020
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
is
the
etiologic
agent
of
COVID-19
pandemic.
Although
other
diagnostic
methods
have
been
introduced,
detection
viral
genes
on
oro-
and
nasopharyngeal
swabs
by
reverse-transcription
real
time-PCR
(rRT-PCR)
assays
still
gold
standard.
Efficient
RNA
extraction
a
prerequisite
for
downstream
performance
rRT-PCR
assays.
Currently,
several
automatic
that
include
are
available.
However,
due
to
growing
demand,
shortage
in
kit
supplies
could
be
experienced
labs.
For
these
reasons,
use
different
commercial
or
in-house
protocols
may
increase
possibility
analyze
high
number
samples.
Herein,
we
compared
efficiency
three
kits
an
protocol
using
synthetic
ssRNA
standards
SARS-CoV-2
as
well
oro-nasopharyngeal
from
six
COVID-19-positive
patients.
It
was
concluded
tested
can
used
with
some
modifications
genome
approaches,
although
differences
yields.
Conversely,
EXTRAzol
reagent
less
efficient
phase
separation
principle
at
basis
extraction.
Overall,
this
study
offers
alternative
suitable
manually
extract
taken
into
account
detection.
Viruses,
Journal Year:
2023,
Volume and Issue:
15(3), P. 599 - 599
Published: Feb. 22, 2023
Emerging
infectious
diseases
of
zoonotic
origin
are
an
ever-increasing
public
health
risk
and
economic
burden.
The
factors
that
determine
if
when
animal
virus
is
able
to
spill
over
into
the
human
population
with
sufficient
success
achieve
ongoing
transmission
in
humans
complex
dynamic.
We
currently
unable
fully
predict
which
pathogens
may
appear
humans,
where
what
impact.
In
this
review,
we
highlight
current
knowledge
key
host–pathogen
interactions
known
influence
spillover
potential
a
particular
focus
on
two
important
viruses
origin,
Nipah
Ebola
virus.
Namely,
determining
include
cellular
tissue
tropism,
as
well
virulence
pathogenic
characteristics
pathogen
capacity
adapt
evolve
within
novel
host
environment.
also
detail
our
emerging
understanding
importance
steric
hindrance
cell
by
viral
proteins
using
“flytrap”-type
mechanism
protein
amyloidogenesis
could
be
crucial
developing
future
antiviral
therapies
against
pathogens.
Finally,
discuss
strategies
prepare
for
reduce
frequency
occurrences
order
minimize
new
outbreaks.
Materials,
Journal Year:
2021,
Volume and Issue:
14(7), P. 1577 - 1577
Published: March 24, 2021
Hand
hygiene
can
be
considered
a
strategic
key
useful
in
the
containment
of
infections
such
as
COVID-19
both
at
home
and
communities
because
it
dramatically
reduce
widespread
outbreak
infections.
In
case
unavailability
soap
water,
“instant”
hand
sanitizers
are
recommended
their
application
easy,
versatile,
quick
often
less
aggressive
for
skin.
For
these
reasons,
alcoholic
alcohol-free
rub
gels
best
performing
formulations
on
market.
Together
with
disinfectants
antiseptic
agents,
hydrogels
play
fundamental
role
obtaining
stable
easy
to
disperse,
pleasant
skin
feel
an
overall
good
performance.
Several
compounds
commonly
used
pharmaceutical,
cosmetic
food
industry
available
this
purpose,
particular,
cellulose
derivatives
synthetic
polymers
derivatives.
Each
them
is
several
grades,
presenting
different
thickening
behavior,
rheological
properties
compatibility
other
ingredients,
alcohols
particular.
all
important
explore
hydrogel
behaviors
contexts
(i.e.,
hydroalcoholic
aqueous
media)
order
develop
new
gels,
always
taking
into
account
international
legal
frameworks
regarding
disinfectant
sanitizing
formulations.
European Journal of Pain,
Journal Year:
2021,
Volume and Issue:
26(3), P. 680 - 694
Published: Dec. 6, 2021
Pain
is
a
clinical
feature
of
COVID-19,
however,
data
about
persistent
pain
after
hospital
discharge,
especially
among
ICU
survivors
scarce.
The
aim
this
study
was
to
explore
the
incidence
and
characteristics
new-onset
its
impact
on
Health-Related
Quality
Life
(HRQoL),
quantify
presence
mood
disorders
in
critically
ill
COVID-19
survivors.This
preliminary
report
PAIN-COVID
trial
(NCT04394169)
presenting
descriptive
analysis
survivors,
following
person
interview
1
month
discharge.
assessed
using
Brief
Inventory,
Douleur
Neuropathique
4
questionnaire
Catastrophizing
Scale.
HRQoL
evaluated
with
EQ
5D/5L,
Hospital
Anxiety
Depression
Scale
(HADS).From
27
May
19
July
2020,
203
patients
were
consecutively
screened
for
eligibility,
65
included
analysis.
Of
these,
50.8%
reported
pain;
38.5%
clinically
significant
(numerical
rating
score
≥3
average
intensity);
16.9%
neuropathic
4.6%
catastrophizing
thoughts,
44.6%
≥2
body
sites
7.7%
widespread
pain.
Patients
had
worse
EQ-VAS
index
value
(p
<
0.001).
intensity
negatively
correlated
both
former
(Spearman
ρ:
-0.546,
p
0.001)
latter
-0.387,
=
HADS
anxiety
depression
values
equal
or
above
eight
obtained
10.8%
patients,
respectively.New-onset
frequent,
it
associated
lower
HRQoL.
Trial
registration
No.:
NCT04394169.
Registered
2020.
https://clinicaltrials.gov/ct2/show/NCT04394169.A
substantial
proportion
severe
may
develop
pain,
post-intensive
care
syndrome
chronic
ICU-related
Given
number
infections
worldwide
unprecedented
size
population
critical
illness
providing
information
characteristics,
influence
patients'
quality
life
might
help
establish
improve
management
strategies.
International Journal of Environmental Research and Public Health,
Journal Year:
2020,
Volume and Issue:
17(21), P. 7730 - 7730
Published: Oct. 22, 2020
The
transmission
of
the
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
and
severity
related
disease
(COVID-19)
are
influenced
by
a
large
number
factors.
This
study
aimed
to
investigate
correlation
COVID-19
case
death
rates
with
possible
causal
climatological
sociodemographic
factors
for
March
May
2020
(first
wave)
period
in
worldwide
scale
statistically
processing
data
over
one
hundred
countries.
weather
parameters
considered
herein
were
air
temperature,
relative
humidity,
cumulative
precipitation,
cloud
cover,
while
included
population
density,
median
age,
government
measures
response
pandemic.
results
this
indicate
that
there
is
significant
between
average
atmospheric
temperature
rates,
chi-square
test
p-values
0.001–0.02
range.
Regarding
factors,
an
even
stronger
dependence
on
age
(p
=
0.0006–0.0012).
Multivariate
linear
regression
analysis
using
Lasso
forward
stepwise
approach
revealed
ranks
first
importance
among
examined
variables,
followed
delays
taking
governmental
or
issuing
stay-at-home
orders.
Immunobiology,
Journal Year:
2021,
Volume and Issue:
226(3), P. 152093 - 152093
Published: May 1, 2021
In
order
to
study
the
mechanisms
of
COVID-19
damage
following
complement
activation
phase
occurring
during
innate
immune
response
SARS-CoV-2,
CR1
(the
regulating
factor,
CD35,
C3b/C4b
receptor),
C4d
deposits
on
Erythrocytes
(E),
and
products
C3b/C3bi,
were
assessed
in
52
patients
undergoing
O
