High-resolution within-sewer SARS-CoV-2 surveillance facilitates informed intervention DOI Creative Commons
Katelyn Reeves, Jennifer Liebig,

Antonio Feula

et al.

Water Research, Journal Year: 2021, Volume and Issue: 204, P. 117613 - 117613

Published: Aug. 28, 2021

To assist in the COVID-19 public health guidance on a college campus, daily composite wastewater samples were withdrawn at 20 manhole locations across University of Colorado Boulder campus. Low-cost autosamplers fabricated in-house to enable an economical approach this distributed study. These sample stations operated from August 25th until November 23rd during fall 2020 semester, with 1512 collected. The concentration SARS-CoV-2 each was quantified through two comparative reverse transcription quantitative polymerase chain reactions (RT-qPCRs). methods distinct utilization technical replicates and normalization endogenous control. (1) Higher temporal resolution compensates for supply or other constraints that prevent biological replicates. (2) data normalized by control agreed raw data, minimizing utility normalization. values reflected prevalence campus as detected clinical services. Overall, combining low-cost sampler method quantifies signal within six hours enabled actionable time-responsive delivered key stakeholders. With reporting findings, surveillance assisted decision making critical phases pandemic detecting individual cases populations ranging 109 2048 individuals monitoring success on-campus interventions.

Language: Английский

Averting an Outbreak of SARS-CoV-2 in a University Residence Hall through Wastewater Surveillance DOI Creative Commons
Ryland Corchis-Scott, Qiudi Geng, Rajesh Seth

et al.

Microbiology Spectrum, Journal Year: 2021, Volume and Issue: 9(2)

Published: Oct. 7, 2021

A wastewater surveillance program targeting a university residence hall was implemented during the spring semester 2021 as proactive measure to avoid an outbreak of COVID-19 on campus. Over period 7 weeks from early February through late March 2021, originating collected grab samples 3 times per week. During this time, there no detection SARS-CoV-2 by reverse transcriptase quantitative PCR (RT-qPCR) in stream. Aiming obtain sample more representative community, decision made use passive samplers beginning onwards. Adopting Moore swab approach, detected just 2 days after were deployed. These also tested positive for B.1.1.7 (Alpha) variant concern (VOC) using RT-qPCR. The result triggered public health case-finding response, including mobile testing unit deployed following day, with nearly 200 students and staff, which identified two laboratory-confirmed cases Alpha COVID-19. individuals relocated separate quarantine facility, averting Aggregating clinical data, campus has yielded first estimates fecal shedding rates VOC nonclinical setting. IMPORTANCE Among adopters monitoring have been colleges universities throughout North America, many whom are approach monitor congregate living facilities evidence infection integral component screening programs. Yet, while numerous examples where among community members, few response that may averted actual outbreak. This report details wastewater-testing when mounting globally over emergence variants concern, reported be transmissible than wild-type Wuhan strain. In communication, we present clear example how resulted actionable responses administration health,

Language: Английский

Citations

75
Making waves: Wastewater surveillance of SARS-CoV-2 in an endemic future DOI
Fuqing Wu, Wei Lin Lee, Hongjie Chen

et al.

Water Research, Journal Year: 2022, Volume and Issue: 219, P. 118535 - 118535

Published: May 3, 2022

Language: Английский

Citations

65
Wastewater Surveillance during Mass COVID-19 Vaccination on a College Campus DOI Open Access
Aaron Bivins, Kyle Bibby

Environmental Science & Technology Letters, Journal Year: 2021, Volume and Issue: 8(9), P. 792 - 798

Published: Aug. 23, 2021

The suitability of wastewater monitoring following widespread vaccination against COVID-19 remains uncertain. SARS-CoV-2 RNA levels were monitored in solids during a university mass campaign which >90% the 12280 students fully vaccinated (Pfizer-BioNTech, BNT162b2). correlated with 7-day average cases when lagged by 1-3 days (ρ = 0.51-0.55; p 0.023-0.039). During and after second vaccine dose, was not detected on 19 21 (12 consecutive nondetection at end semester), significant decrease (p 0.027) positivity rate. A large influx outside visitors (move out commencement) led to an immediate increase (seven detections over seven days). Wastewater offer sensitive matrix for environmental surveillance subsewershed level (50% probability detection two cases) both vaccination. Mass coincident decreased shedding into wastewater. This suggests absence population infections, symptomatic or not, among campus population.

Language: Английский

Citations

61
Passive sampling to scale wastewater surveillance of infectious disease: Lessons learned from COVID-19 DOI Creative Commons
Aaron Bivins, Devrim Kaya, Warish Ahmed

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 835, P. 155347 - 155347

Published: April 21, 2022

Much of what is known and theorized concerning passive sampling techniques has been developed considering chemical analytes. Yet, historically, biological analytes, such as Salmonella typhi, have collected from wastewater via with Moore swabs. In response to the COVID-19 pandemic, re-emerging a promising technique monitor SARS-CoV-2 RNA in wastewater. Method comparisons disease surveillance using composite, grab, for detection found variety materials routinely produced qualitative results superior grab samples useful sub-sewershed COVID-19. Among individual studies, concentrations derived samplers demonstrated heterogeneous correlation paired composite ranging weak (R2 = 0.27, 0.31) moderate 0.59) strong 0.76). sampler materials, electronegative membranes shown great promise linear uptake observed exposure durations 24 48 h several cases positivity on par samples. Continuing development methods infectious diseases diverse forms fecal waste should focus optimizing efficient recovery kit-free extraction, resource-efficient testing capable rapidly producing or quantitative data. With refinements could prove be fundamental tool scaling disease, especially among 1.8 billion persons living low-resource settings served by non-traditional collection infrastructure.

Language: Английский

Citations

57
Sensitivity of wastewater-based epidemiology for detection of SARS-CoV-2 RNA in a low prevalence setting DOI
Joanne Hewitt, Sam Trowsdale, Bridget Armstrong

et al.

Water Research, Journal Year: 2022, Volume and Issue: 211, P. 118032 - 118032

Published: Jan. 2, 2022

Language: Английский

Citations

55
Use of sewage surveillance for COVID-19 to guide public health response: A case study in Hong Kong DOI Creative Commons
Yu Deng, Xiaoqing Xu,

Xiawan Zheng

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 821, P. 153250 - 153250

Published: Jan. 19, 2022

Sewage surveillance could help develop proactive response to the Coronavirus Disease 2019 (COVID-19) pandemic, but currently there are limited reports about examples in practical exercises. Here, we report a use case of intensified sewage initiate public health action thwart looming Delta variant outbreak Hong Kong. On 21 June 2021, albeit under basically contained COVID-19 situation Kong, routine identified high viral load severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sample from one site covering over 33,000 population, suggesting infected cases living respective sewershed. The newly developed method based on allele-specific real-time quantitative polymerase chain reaction (AS RT-qPCR) served alert first documentation local community three days before was confirmed be carrier. Intensified triggered. Targeted upstream sampling at sub-sewershed areas pinpointed source positive signal across spatial scales sewershed building level, and assisted determining specific area for issuing compulsory testing order individuals 23 2021. A person who lived with result 24 Viral genome sequences determined were compared those clinic specimens matched patient, that SARS-CoV-2 sample. This study build confidences agencies using their own communities.

Language: Английский

Citations

50
Comparison of high-frequency in-pipe SARS-CoV-2 wastewater-based surveillance to concurrent COVID-19 random clinical testing on a public U.S. university campus DOI
Jillian Wright, Erin M. Driver, Devin A. Bowes

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 820, P. 152877 - 152877

Published: Jan. 6, 2022

Language: Английский

Citations

50
SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings DOI Creative Commons
Mitchell D. Ramuta, Christina M. Newman,

Savannah F. Brakefield

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 11, 2022

Abstract Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess risk transmission in congregate spaces. We deployed active air samplers monitor presence SARS-CoV-2 real-world settings across communities Upper Midwestern states Wisconsin and Minnesota. Over 29 weeks, we collected 527 samples from 15 settings. detected 106 that were positive viral RNA, demonstrating can be continuous variety expanded utility surveillance test 40 other respiratory pathogens. Surveillance data revealed differences timing location influenza A virus detection. In addition, obtained genome sequences identify variant lineages. Collectively, this shows sampling scalable, high throughput tool could used conjunction with methods detecting pathogens

Language: Английский

Citations

50
Monitoring of SARS-CoV-2 in sewersheds with low COVID-19 cases using a passive sampling technique DOI Open Access
Jiaying Li, Warish Ahmed, Suzanne Metcalfe

et al.

Water Research, Journal Year: 2022, Volume and Issue: 218, P. 118481 - 118481

Published: April 20, 2022

Language: Английский

Citations

49
Evaluation of a Wastewater-Based Epidemiological Approach to Estimate the Prevalence of SARS-CoV-2 Infections and the Detection of Viral Variants in Disparate Oregon Communities at City and Neighborhood Scales DOI Creative Commons
Blythe A. Layton, Devrim Kaya, Christine Kelly

et al.

Environmental Health Perspectives, Journal Year: 2022, Volume and Issue: 130(6)

Published: June 1, 2022

Positive correlations have been reported between wastewater SARS-CoV-2 concentrations and a community's burden of infection, disease or both. However, previous studies mostly compared to clinical case counts nonrepresentative convenience samples, limiting their quantitative potential.

Language: Английский

Citations

49